• The Korean Journal of Mycology
• /
• v.27 no.4 s.91
• /
• pp.304-311
• /
• 1999

Batch kinetics during the exo-polysaccharide (EPS) fermentation of Ganoderma lucidum was investigated as a function of different substrates (glucose and starch), substrate concentration $(1{\sim}7%,\;w/v)$ and subculture (3 times). Logistic model for mycelial growth fitted the experimental data better than Monod and two thirds power model. The Luedeking-Pirt equation was adequate to fit the kinetic data of product formation and substrate consumption. The EPS production was strongly non-growth associated, although it was mixed type. The product formation and sustrate consumption by growth associated mechanism decreased as the concentration of glucose increased, while those of the non-growth associated mechanism increased. However, starch medium increased the growth associated and non-growth associated substrate consumption indicating higher availability of substrate. Also, batch culture in starch medium showed the higher specific growth rate and stability during subculture than those in glucose medium. In conclusion, the enhanced EPS production and stability in the subculture was found to be remarkably improved by use of starch as sole carbon source in medium. The maximum mycelium dry weight and EPS production of 9.463 and 10.410 g/l, respectively, were obtained after shake culture of 7 days at $30^{\circ}C$ from the media containing 7% starch.

• The Korean Journal of Mycology
• /
• v.39 no.2
• /
• pp.99-104
• /
• 2011

Shiitake breeding requires the procedures of mating of two different parental strains and selection of hybrid strains that have good traits for the mushroom production. In this study, we tested the possibility of the use of chromogenic plate-based assay for extracellular enzyme production in order to assess and find good biochemical properties-possessed hybrid strains that were generated from genetic cross of the monokaryotic strains derived from two different parental strains of Lentinula edodes Sanjo-101ho and Sanjo-108ho. We observed that there was difference in the ability of producing ${\beta}$-glucosidase, avicelase, CM-cellulase, amylase, pectinase, xylanase, and protease among the monokaryotic strains. We could also comparatively assess that the ability of the seven extracellular enzymes production in the hybrid strains depended on the mating combination of the monokaryotic strains. Our results demonstrate that the assessment method for extracellular enzyme production using chromogenic plate assay could be usefully applied to the assessment of the hybrid strains derived from the breeding procedure of L. edodes.

• Journal of Sasang Constitutional Medicine
• /
• v.18 no.3
• /
• pp.64-74
• /
• 2006

1. Objectives and Methods A Study on the Characteristics of the Korean Adult Male Sound according to Sasang Constitution using PSSC with a Sentence. Sasang Constitutional Medicine(SCM) is the one of the traditional Korean Medicine. It classifies people into four categories like Taeyangin, Soyangin, Taeumin and Soeumin. The rule to classify is Appearance and Body Shape, Facial Appearance and Speech, Character and Talents and Diseases and Medications. This study was done to investigate the relationships between Voice and Sound parameters using PSSC(Phonetic System of Sasang Constitution) in a sentence. Experimental Participants were 195 Korean adult males including 1 Taeyangin, 37 Soyangin, 105 Taeumin and 52 Soeumin. Sasang Constitutional specialist used PSSC and Korean Medical Diagnosis to classify participants into four constitution. 2. Results In Pitch segment, Soyangin's Center freq.(4) was significantly high compared with Taeyangin and Taeumin groups. Soyangin's and Soeumin's Center freq.(6) was significantly high compared with Taeyangin and Taeumin groups. In APQ segment and Octave segment, there were no significant differences among four groups. In Shimmer segment, Taeumin's F Shimmer(1) and F Shimmer(2) were significantly high compared with Taeyangin and Soyangin groups. In Energy segment, Taeyangin's 2k-4k total sum, 2k-4k dev., C dev., C# dev. and D S.D. were significantly high compared with other groups. In Recording time segment, there was no significant difference among four groups. More Taeyangin cases and the other parameters are needed to determine constitution using PSSC and to make PSSC effective. 3. Conclusions From above result, there is the possibility of efficiency standard guide for constitution diagnosis by analyzation og voice.

• Journal of Life Science
• /
• v.25 no.2
• /
• pp.189-196
• /
• 2015

Bacterial diversity was studied in the rhizosphere of Suaeda japonica Makino, which is native to Suncheon Bay in South Korea. Soil samples from several sites were diluted serially, and pure isolation was performed by subculture using marine agar and tryptic soy agar media. Genomic DNA was extracted from 29 pure, isolated bacterial strains, after which their 16S rDNA sequences were amplified and analyzed. Phylogenetic analysis was performed to confirm their genetic relationship. The 29 bacterial strains were classified into five groups: phylum Firmicutes (44.8%), Gamma proteobacteria group (27.6%), Alpha proteobacteria group (10.3%), phylum Bacteriodetes (10.3%), and phylum Actinobacteria (6.8%). The most widely distributed genera were Bacillus (phylum Firmicutes), and Marinobacterium, Halomonas, and Vibrio (Gamma proteobacteria group). To confirm the bacterial diversity in rhizospheres of S. japonica, the diversity index was used at the genus level. The results show that bacterial diversity differed at each of the sampling sites. These 29 bacterial strains are thought to play a major role in material cycling at Suncheon Bay, in overcoming the sea/mud flat-specific environmental stress. Furthermore, some strains are assumed to be involved in a positive interaction with the halophyte S. japonica, as rhizospheric flora, with induction of growth promotion and plant defense mechanism.

• Journal of Life Science
• /
• v.19 no.8
• /
• pp.1033-1038
• /
• 2009

Maximal productions of gellan by Sphingomnas paucibilis NK2000 from 20 g/l glucose and 10 g/l soybean pomace were 7.46 g/l in a flask and 7.35 g/l in a 7 l bioreactor, when the initial pH of media was 6.8. Maximal production of gellan in a 7 l bioreactor under pH control by sodium hydroxide was 8.42 g/l, whereas that under control by potassium phosphate was 8.50 g/l. The optimal concentration of potassium phosphate in a medium for production of gellan by S. paucibilis NK2000 was found to be 5.0 g/l. Maximal production of gellan in a medium containing 5.0 g/l potassium phosphate without pH control was 8.93 g/l in a 7 l bioreactor. In this study, a simple process without pH control was developed to enhance the production of gellan, with optimized concentration of potassium phosphate in the medium.

• Korean Journal of Environment and Ecology
• /
• v.18 no.2
• /
• pp.249-262
• /
• 2004

This study was conducted to suggest alternatives for the effective use of national park visitor centers, by evaluating their functionality. Visitor centers in Naejangsan National Park and Pukhansan National Park, which are deliberately composed and managed, were investigated for this purpose. First, I clarified the concept, function and type of a visitor center through literature review, and then, analyzed visitors' post-visiting evaluation based on McManus's Communication Theory. The results of the analysis shows that visitors have not enough time to understand the contents of visitor centers, and facilities and guide sign system to support visitors are insufficient, which seem to disturb the proper communication between visitors and exhibits. Visitors' short visiting time caused by their little interest in exhibits and their low degree of understanding show the difficulties in the communication between visitors and exhibits. The following alternatives are suggested to improve these problems: first, the improvement of the guide sign system; second, the provision of exhibition rooms specialized for different visitor groups; third, the display of exhibits connected with visitors' experiences; Forth, the utilization of various media proper for each subject.

• Korean Journal of Occupational Health Nursing
• /
• v.17 no.1
• /
• pp.23-33
• /
• 2008

Purpose: The purpose of the study was to identify attitudes of middle managers toward employees with psychological problems and to determine factors affecting their attitudes. Methods: A questionnaire with Community Attitudes Toward Mentally Ill (CAMI) scales was administered to 161 middle managers working in small and medium-sized enterprises based in Seoul and Gyeonggi Province. Results: There are four separate subscales on the CAMI. Mean score for authoritarianism was $35.0{\pm}4.4$, benevolence $23.0{\pm}4.8$, social restrictiveness $32.3{\pm}4.9$ and community mental health ideology $27.2{\pm}5.1$ According to multiple regression analysis, middle managers with no experience of learning mental illness through mass media or higher levels of depression symptom were more authoritative and less benevolent towards employees with psychological problems. The experience of meeting a patient with mental problem contribute to positive attitudes toward people with mental illnesses in social restrictiveness subscale and community mental health ideology subscale among CAMI. Conclusion: This study suggests that experience of having patients with mental problems and information on psychological problems will have great influence on attitudes of middle managers toward employees with psychological problems. It might be important to help middle manager manage their depression because their depression also affects their attitudes toward employees with psychological problems.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.18 no.9
• /
• pp.2109-2116
• /
• 2014

This paper presents the implementation and design of the advanced WI-FI systems with beam-forming antenna that radiate their power to the direction of user equipment to improve the overall throughput, contrast to the general WI-FI systems equipped with omni-antenna. The system consists of patch array antenna, DSP, FPGA, and Qualcomm's commercial chip. The beam-forming system on the FPGA utilizes the packet information from Qualcomm's commercial chip to control the phase shifters and attenuators of the patch array antenna. The PCI express interface has been used to maximize the communication speed between DSP and FPGA. The directions of arrival of users are managed using the database, and each user is distinguished by the MAC address given from the packet information. When the system wants to transmit a packet to one user, it forms beams to the direction of arrival of the corresponding user stored in the database to maximize the throughput. Directions of arrival of users are estimated using the received preamble in the packet to make its SINR as high as possible. The proposed beam-forming system was implemented using an FPGA and Qualcommm's commercial chip together. The implemented system showed considerable throughput improvement over the existing general AP system with omni-directional antenna in the multi-user communication environment.

• Journal of Broadcast Engineering
• /
• v.13 no.2
• /
• pp.179-187
• /
• 2008

In this paper, we proposed the qualify metric based on SVC and the subjective quality. The proposed quality metric is for a general purpose. It means we can use it for any video sequences regardless of its temporal and spatial characteristics. The Quality of Service(QoS) is one of the important issues in heterogeneous environment which has diverse restrictions such as limited network bandwidth and limited display resolution. Scalable Video Coding(SVC) is the efficient video coding skill in heterogeneous environment. Because SVC can be adapted to various quality bitstreams using three scalabilities(spatial, temporal, and SNR) from one bitstream which has full scalability. To maximize the QoS in this environment, we should consider the subjective quality which is the viewer response. And also we should consider temporal and spatial characteristics of video sequence because the subjective quality is affected by temporal and spatial characteristics of video sequence. To verify the efficiency of the proposed method, we perform subjective assessments. The experimental results show that the proposed method has high correlation with subjective quality. The proposed method can be a decision tool of SVC birstream extraction.

• Journal of Korean Society of Occupational and Environmental Hygiene
• /
• v.6 no.2
• /
• pp.209-221
• /
• 1996

The purpose of this study was to find a suitable cosolvent to $CS_2$ so that desorption efficiency can be improved for both polar and non-polar organic solvent mixtures collected on an activated charcoal tube. Cosolvents added to $CS_2$ include: DMF(N,N-dimethylformamide): $CS_2$ (v/v 1:99), DMF:$CS_2$(v/v 3:97), BC (butyl carbitol, 2-(2-butoxy ethoxy) ethanol):$CS_2$(v/v 1:99), and BC:$CS_2$(v/v 3:97)). The results obtained were as follows : 1. Comparing the desorption efficiency of $CS_2$ with those of $CS_2$ with 1, 3, 5 % DMF and 1, 3 % BC cosolvents for two different groups of charcoal tubes each containing 8 different polar and non-polar organic solvents with 3 different concentration levels, the desorption efficiencies of the cosolvent-added $CS_2$ increased significantly for all polar organic solvents regardless of concentration levels tested. For non-polar organic solvents, no noticeable improvement was detected except xylene and trichloroethylene. The desorption efficiency of xylene increased significantly while that of trichloroethylene increased significantly at the lowest concentration level tested. 2. Either 5 % DMF or 3 % BC was the most suitable cosolvent because the desorption efficiency for non-polar organic solvent mixtures was similar or slightly improved compared with that of $CS_2$, while those of for polar organic solvent mixtures were above 75 % except for cyclohexanone. 3. The smallest variations in desorption efficiency represented by the ratio calculated from the maximum to minimum desorption efficiency for all concentration levels tested were found when 3 % BC was used as a cosolvent. The above results indicate that the desorption efficiency of $CS_2$ particularly for polar organic solvent mixtures collected on a charcoal tube can be significantly improved by the use of cosolvents such as 5 % DMF or 3 % BC. A caution, however, is in order for selecting a cosolvent whenever the cosolvent itself is being used in the workplace or the impurities contained in the cosolvent may interfere with the analytical results. In addition, to improve desorption efficiencies for such organic solvents as cyclohexanone or ketones, it is recommended to use suitable collection and desorption media other than the traditional method of charcoal tube collection/$CS_2$ desorption.