The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지

Batch Kinetics of Exo-polysaccharide Production by Submerged Cultivation of Ganoderma lucidum

영지의 액체배양에 의한 세포외 다당 생산의 동력학적 특성

  • Lee, Shin-Young (Division of Environmental and Biological Engineering, Kangwon National University, Bioproducts Research Center of Yousei University) ;
  • Lee, Hak-Su (Division of Environmental and Biological Engineering, Kangwon National University, Bioproducts Research Center of Yousei University) ;
  • Park, Heung-Cho (Department of Polymer Engineering, Chungju National University)
  • 이신영 (강원대학교 환경생물공학부 및 연세대학교 생물산업소재연구센터) ;
  • 이학수 (강원대학교 환경생물공학부 및 연세대학교 생물산업소재연구센터) ;
  • 박흥조 (충주산업대학교 고분자공학과)
  • Published : 1999.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

Batch kinetics during the exo-polysaccharide (EPS) fermentation of Ganoderma lucidum was investigated as a function of different substrates (glucose and starch), substrate concentration $(1{\sim}7%,\;w/v)$ and subculture (3 times). Logistic model for mycelial growth fitted the experimental data better than Monod and two thirds power model. The Luedeking-Pirt equation was adequate to fit the kinetic data of product formation and substrate consumption. The EPS production was strongly non-growth associated, although it was mixed type. The product formation and sustrate consumption by growth associated mechanism decreased as the concentration of glucose increased, while those of the non-growth associated mechanism increased. However, starch medium increased the growth associated and non-growth associated substrate consumption indicating higher availability of substrate. Also, batch culture in starch medium showed the higher specific growth rate and stability during subculture than those in glucose medium. In conclusion, the enhanced EPS production and stability in the subculture was found to be remarkably improved by use of starch as sole carbon source in medium. The maximum mycelium dry weight and EPS production of 9.463 and 10.410 g/l, respectively, were obtained after shake culture of 7 days at $30^{\circ}C$ from the media containing 7% starch.

영지버섯의 세포외 다당발효중 동력학적 특성을 기질(포도당, 전분), 기질농도$(1{\sim}7%)$ 및 계대배양(3회)의 함수로서 조사하였다. 영지버섯 균사체 증식은 logistic 모델이 Monod 모델 및 two- thirds power 모델과 비교하여 실험값에 잘 일치하였고, 기질 및 생성물은 Luedeking-Pirt 식에 의하여 잘 설명되었다. 또 다당 생성의 발효 기작은 증식연동형과 비증식 연동형이 함께 존재하는 혼합형이었으나 기질에 상관없이 비증식연동형 기작이 더 중요하였다. Glucose는 농도 증가에 따라 다당생성 및 기질소비의 기작이 증식연동형이 감소하고 비증식연동형이 증가하는 경향을 보였다. 그러나 starch를 사용하였을 경우는 glucose와는 달리, 기질소비의 증식연동형과 비증식연동형 기작이 모두 증가하여 높은 기질 이용성을 보였다. 아울러 starch배지에서는 glucose배지에서 보다 비증식속도의 증가와 계대배양시의 안정성을 보였다. 따라서 영지버섯의 배양시 starch배지는 비증식연동형에 의한 균체 생육 및 다당생성의 생합성 촉진에 의해 이들 생산성 및 계대배양시의 안정성을 증가시키는 것으로 생각되었다 최대의 균사체 생육 및 다당 생산은 각각 9.463 및 10.410 g/l로, 7% starch을 함유한 배지에서 $30^{\circ}C$로 7일간 진탕배양하였을 때 얻어졌다.

Keywords

References

  1. Biochemical Engineering Aiba, S.;Humphrey, A.E.;Millis, N.F.
  2. Biochemical Engineering Fundamentals Bailey, J.E.;Ollis, D.F.
  3. Biotechnol. Bioeng. v.30 Pullulan from peat hydrolyzate fermentation kinetics Boa, J.M.;LeDuy, A.
  4. Appl. Microbiol. Biotechnol. v.25 The effect of ammonium ions and pH on the elaboration of the fungal extracellular polysaccharide, pullulan, by Aureobasidium pullulans Bulmer, M.C.;Catley, B.J.;Kelly, P.J.
  5. Appl. Environm. Microbiol. v.33 no.4 Exponential growth kinetics for Polyporus versicolor and Pleurotus ostreatus in submerged culture Carroad, P.A.;Wilke, C.R.
  6. Mushroom Mycelium Grown in Submerged Culture - Potential Food Applications;Biotechnology and Food Ingredients Eyal, J.;Goldberg, I.(ed.);Williams, R.(ed.)
  7. Adv. Appl. Microbiol. v.23 Mathematical models for fermentation processes Fredrickson, A.C.;Megee, R.D.;Tsuchiya, H.M.
  8. Adv. Appl. Microbiol. v.37 Medicinal benefits of the mushroom Ganoderma Jong, S.C.;Birmingham, J.M.
  9. J. lmmunol. lmmunopharmacol. v.11 Immuno-modulatory substances of fungal origin Jong, S.C.;Birmingham, J.M.;Pai, S.H.
  10. Biotech. Bioeng. v.22 Extracellular microbial polysaccharides: Kinetics of Pseudomonas sp., Azotobacter vinelandii, and Aureobasidium pullulans batch fermentaion Klimek, J.;Ollis, D.F.
  11. Kor. J. Appl. Microbiol. Biotechnol. v.24 no.1 Production conditions and characterization of exo-biopolymer produced by submerged cultivation of Ganoderma lucidum mycelium Lee, S.Y.;Kang, T.S.
  12. Kor. J. Biotech. Bioeng. v.12 no.2 Optimization of antitumor active exo-polysaccharide production through the submerged cultivation of Ganoderma lucidum mycelium Lee, S.Y.;Kang, T.S.
  13. Kor. J. Biotech. Bioeng. v.13 no.5 Condition of exopolysaccharide production from submerged mycelial culture of Ganoderma lucidum by using air-lift fermenter system Lee, S.Y.;Kang, T.S.;Lee, M.C.
  14. J. Biochem. Microbiol. Tech. & Eng. v.1 A kinetic study of the lactic acid fermentation. Batch processes at controlled pH Luedeking, R.;Piret, E.L.
  15. Analytical Chem. v.31 Use of dinitrosalicylic acid reagent for determination of reducing sugar Miller, G.L.
  16. Nippon Nogeikagaku Kaishi v.58 Fractionation, structural feastures and antitumor activity of water-soluble polysaccharides from Reishi, the fruitbody of Ganoderma lucidum Mizuno, T.;Kato, M.;Totsuka, K.;Takenaka, K.;Shinkai, K.;Shimizu, M.
  17. Biotech. Bioeng. v.32 Batch kinetics of microbial polysaccharide biosynthesis Mulchandani, A.;Luong, J.H.T.
  18. Starch v.27 A modified analysis method of starch determination by iodine spectrophotometry Nedeltscheva, M.;Stoilkov, G.;Popova, S.
  19. Ann. N.Y. Acad. Sci. v.413 Simple batch fermentation model: Theme and variations Ollis, D.F.
  20. Agric. Biol. Chem. v.52 no.2 Effect of carbon and nitrogen sources on submerged culture of edible fungi Sakamoto, R.;Niimi, T.;Takahashi, S.
  21. Biotechnol. Bioeng. v.12 Introductory comments Tsuchiya, H.M.
  22. Biotech. Bioeng. v.22 Extracellular microbial polysaccharides. I. Substrate, biomass, and product kinetic equations for natch xanthan gum fermentaion Weiss, R.M.;Ollis, D.F.