• Title/Summary/Keyword: meat-type

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Comparative analysis on genome-wide DNA methylation in longissimus dorsi muscle between Small Tailed Han and Dorper×Small Tailed Han crossbred sheep

  • Cao, Yang;Jin, Hai-Guo;Ma, Hui-Hai;Zhao, Zhi-Hui
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.11
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    • pp.1529-1539
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    • 2017
  • Objective: The objective of this study was to compare the DNA methylation profile in the longissimus dorsi muscle between Small Tailed Han and Dorper${\times}$Small Tailed Han crossbred sheep which were known to exhibit significant difference in meat-production. Methods: Six samples (three in each group) were subjected to the methylated DNA immunoprecipitation sequencing (MeDIP-seq) and subsequent bioinformatics analyses to detect differentially methylated regions (DMRs) between the two groups. Results: 23.08 Gb clean data from six samples were generated and 808 DMRs were identified in gene body or their neighboring up/downstream regions. Compared with Small Tailed Han sheep, we observed a tendency toward a global loss of DNA methylation in these DMRs in the crossbred group. Gene ontology enrichment analysis found several gene sets which were hypomethylated in gene-body region, including nucleoside binding, motor activity, phospholipid binding and cell junction. Numerous genes were found to be differentially methylated between the two groups with several genes significantly differentially methylated, including transforming growth factor beta 3 (TGFB3), acyl-CoA synthetase long chain family member 1 (ACSL1), ryanodine receptor 1 (RYR1), acyl-CoA oxidase 2 (ACOX2), peroxisome proliferator activated receptor-gamma2 (PPARG2), netrin 1 (NTN1), ras and rab interactor 2 (RIN2), microtubule associated protein RP/EB family member 1 (MAPRE1), ADAM metallopeptidase with thrombospondin type 1 motif 2 (ADAMTS2), myomesin 1 (MYOM1), zinc finger, DHHC type containing 13 (ZDHHC13), and SH3 and PX domains 2B (SH3PXD2B). The real-time quantitative polymerase chain reaction validation showed that the 12 genes are differentially expressed between the two groups. Conclusion: In the current study, a tendency to a global loss of DNA methylation in these DMRs in the crossbred group was found. Twelve genes, TGFB3, ACSL1, RYR1, ACOX2, PPARG2, NTN1, RIN2, MAPRE1, ADAMTS2, MYOM1, ZDHHC13, and SH3PXD2B, were found to be differentially methylated between the two groups by gene ontology enrichment analysis. There are differences in the expression of 12 genes, of which ACSL1, RIN2, and ADAMTS2 have a negative correlation with methylation levels and the data suggest that DNA methylation levels in DMRs of the 3 genes may have an influence on the expression. These results will serve as a valuable resource for DNA methylation investigations on screening candidate genes which might be related to meat production in sheep.

Supercritical Carbon Dioxide Extraction of Beef and Pork for Low Lipid Sausage Manufacturing (저지방 소시지 제조를 위한 쇠고기와 돼지고기의 초임계 이산화탄소 추출)

  • Kwon, Young-An
    • Korean Journal of Food Science and Technology
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    • v.32 no.2
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    • pp.306-311
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    • 2000
  • Lipid and cholesterol were extracted from beef and pork by the supercritical carbon dioxide $(SC-CO_2)$ for the manufacturing of low-lipid and low-cholesterol sausage. The ranges of extraction temperature and pressure were from 35 to $55^{\circ}C$ and from 103 to 375 bar, respectively. $SC-CO_2$ extraction yield of beef lipid increased as extraction pressure increased and/or extraction temperature decreased, while extraction temperature was more influential on the cholesterol extraction than pressure condition. When lipid and cholesterol of freeze-dried beef with varied moisture contents were extracted, their solubilities increased as the moisture content reduced. The extraction of lipid and cholesterol from pork was shown the same tendency as the beef. The chunk type of beef shape was more suitable for the lipid and cholesterol extraction than the powder type of beef. The color of meat after $SC-CO_2$ extraction was lighter than the raw freeze dried meat because of the extraction of color pigments. After $SC-CO_2$ extraction, beef and pork were rehydrated and mixed with raw beef and pork containing lipid and cholesterol. Their mixing ratio up to 50 : 50 did not affect physical properties of the sausage compared with the control sausage.

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A Study on the Food Habit and Health Status of the Rural Housewives in Gyonggi Province (경기도 농촌지역 주부의 식습관 및 건강상태 실태조사)

  • 박영주;박양자;이연숙
    • Korean Journal of Rural Living Science
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    • v.6 no.2
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    • pp.99-108
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    • 1995
  • This study was carried out to investigate the food habit and health status of housewives in rural area of Gyonggi Province. The subjects of this study were 121 rural housewives and interviewed with a questionnaire. The results were as follows 1. In food habit items, the mean of Kimchi intake ($4.89\pm0.38$) was the highest, while that of meat processed food intake ($2.12\pm1.07$) was the lowest. The average of food habit score was $53.1\pm7.7$(out of 80) and was not significantly different with educational level, income, family type, size and agricultural type. In age, older subjects had low score. In regular mealtime, 74.4% of subjects had high score. The subjects that had good appetite were 49.6%. 2. The average of height and weight were 156.99cm and 55.5kg respectively. The appeal ratio in THI items was high in the order of musculoskeletal pain (1.62), tired (1.88), eye-fatigued (1.91), mental instability (2.03) and depression (2.07). The average of THI score was $32.7\pm5.5$(out of 45) and was not significantly different with educational level, income, family type and size, but the subjects of younger and nonagricultural belonged to high score level. The percentage of subjects in normal blood pressure, obesity and retained disease at present were 66.9%, 6.1% and 23.1% respectively. There was no significant difference except for good appetite in the relation among food habit, obesity and blood pressure. Food habit score was not significantly different with THI score, but health status seemed to be good in the better food habit.

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Development of composite catalyst for hazardrous gas treatment using the heat of aviary heating equipment (계사용 온풍기를 이용한 유해가스처리 복합촉매시스템 개발)

  • Jang, Hyun-Tae;Cha, Wang-Seog
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.10
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    • pp.2779-2785
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    • 2009
  • Among the livestock, chickens are raised because of the merit ingested protein in low-priced cost of production and are primary livestock increased the consumption of meat. The factors of influencing condition, odor is the most important factor. Odor substances are ammonia, amines, hydrogen sulfide and mercaptan which come from night soil. Livestock are prevented from rearing by means of these odor substances. Though the henhouse is heated using hot air type heater in the winter season, it is ventilated for the control of odor because of the increase of odor concentration. In the present work, composite catalytic system combined the existing facilities(hot air type heater) with catalytic system was developed, it could controled odor and hazardous gas using the oxidation/reduction reaction without extra operating cost. Moreover, the purpose of this work is to develop the catalysts which are cost competitive and can maximize energy efficiency. The catalysts are noble metal(Pt-Rh) and composite transition metal(Mn) type.

A Survey on Food Culture of College Student in Seoul Area (서울지역(地域) 대학생(大學生)들의 식문화(食文化)에 대한 의식(意識) 및 실태(實態) 조사연구(調査硏究))

  • Lee, Young-Soon;Lim, Na-Young;Park, Kwang-Hee
    • Journal of the Korean Society of Food Culture
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    • v.9 no.4
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    • pp.369-378
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    • 1994
  • This study was surveyed by 319 male and 336 female college students in Seoul for food behavior and effect of Western food on food life. The results were summarized as follows; 1. The recognition of Korean folk food was higher in female than male students. 2. Eating type of college students appeared that they prefered to cooked rice at breakfast, lunch, and dinner because of a general food type of their family. 3. The favorite food items in college students showed fruits in female and meat in male. 4. Eating out type with family appeared mainly Korean food, because of their preference. 5. The 29% of college students visited fast food restaurants more than $1{\sim}2$ times per week. 6. In compared to Western food, the Korean food were favored by college students but cooking process of food were complicated. 7. The preference of Korean food against Western food was mostly higher in male than female. 8. The standard of food choice were taste and convenience of food. 9. Main factor of food habit change appeared convenience of buying easily. 10. The influence of the Western food in our food behavior were easy to buying but not fit our preference. 11. Opinion of students in order to keep Korean food were follows; Various food development, enlargement of consumption market, correct education about Korean food and change of people consciousness through consumer campaign.

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EFFECTS OF COBALT AND NICKEL ON ZINC AVAILABILITY IN CHICKS AND PIGS FED PRACTICAL-TYPE DIETS HIGH IN CALCIUM

  • Chung, A.S.;Lee, S.T.;Grummer, R.H.;Hoekstra, W.G.
    • Asian-Australasian Journal of Animal Sciences
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    • v.1 no.2
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    • pp.81-88
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    • 1988
  • Experiments were conducted with chicks and pigs to determine whether the sparing effects of cobalt (Co) or nickel (Ni) on zinc (Zn) nutrition were due to an improved Zn availability. They included a Zn balance study with New Hampshire X Leghorn cross chicks, a $^{65}Zn$ absorption study with Ancona chicks and a $^{65}Zn$ balance study with pigs. The basal diet was a corn-soybean type diet high in calcium. In the Zn balance study with New Hampshire X Leghorn cross chicks, Zn retention percentages for basal, + 54 ppm Ni or + 54 ppm Co were 39.4, 40.4 and 48.3, respectively. In the $^{65}Zn$ absorption study with chicks, adding these levels of Ni or Co to the basal diet increased $^{65}Zn$ absorption from 12.8% to 14.0% and 15.1%, respectively. Supplemental Ni and Co increased the proportion of body $^{65}Zn$ found in liver and bone. With the pig experiment, $^{65}Zn$ retention percentages for basal, +54 ppm Co and + 60 ppm Zn groups were 20.0, 26.7 and 12.2, respectively; while Zn retention values (mg) in the body were 29.5, 45.1 and 60.5, respectively. In addition, supplemental Co increased $^{65}Zn$ concentration in the blood, liver, kidney and duodenum. These studies showed that supplementation of the basal diet with Co resulted in increased absorption of dietary Zn in chicks and pigs. The effect of Ni in chicks was less than that of Co. The ability of supplemental Co and to a lesser extent of Ni to improve weight gain as well as reduce other Zn-deficient signs in both species fed a practical corn-soybean type diet high in in calcium can be explained, at least in part by an associated increase in Zn absorption. The possible mechanism involved in these effects are discussed.

Hemeroby Mapping and its Application in Open Space Planning -The Case of Daegu Metropolitan City- (Hemeroby 도면작성과 녹지계획에서의 활용 -대구시를 사례로-)

  • 나정화
    • Journal of the Korean Institute of Landscape Architecture
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    • v.29 no.2
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    • pp.32-41
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    • 2001
  • The purpose of this study is to assess naturalness of each and use type for Daegu and provide the basic data for urban green space planning. The results are as follows: 1) As a result of periodic analysis, It is known that in the 1400´s that the rate of a-/oligo-/meso-hemeroby was over 90% of that area, in which naturalness was very high. By the 2020´s, the population will increase to about 3,500,000 and the occupation rae of meat-and poly-hemeroby, where naturalness is very low, is expected to increase rapidly. 2) The recent land uses of Daegu are divided ito 17 types. In the survey of a occupation rate of each land use type, a forest are occupies 48.8% of the entire area. The military installation zone is lowest with 0.07%. 3) Investigation of a dominant hemeroby grade in each land use type showed that the urban complex area in characterized by meta-hemeroby : and the space for pavement and building construction where naturalness is lowest. On the other hand, oligo-hemeroby that is worth being preserved was surveyed to be dominant in woodlands and natural forests. 4) In the analysis of hemeroby mapping in the process of a green space planning, it is clear that hemeroby will be used a s a tool that assesses the suitability for recreation activity of each sample case. For example, it enables a poly-hemeroby region to be utilized as a near-natural recreation area including $\beta$-and $\alpha$-euhemeroby region in Daegu. Besides, hemeroby can provide basic data to make a value judgement of each sample case with a view to preservation of nature.

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Physicochemical properties of emulsion-type sausage added red yeast rice powder (홍국 분말을 첨가한 유화형 소시지의 이화학 특성 연구)

  • Kim, Cheol-Seon;Kim, Hack-Youn
    • Korean Journal of Food Science and Technology
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    • v.49 no.4
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    • pp.396-400
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    • 2017
  • The quality of emulsion-type sausage manufactured using various amounts of red yeast rice powder (0, 1, 2, 3%) was investigated. Moisture and ash content increased, whereas the protein content decreased, upon increasing the red yeast rice powder content. The pH, lightness, and yellowness values of the uncooked and cooked samples decreased upon increasing the red yeast rice powder content. The redness of the cooked and uncooked samples containing 3% red yeast rice powder was higher than those of others. Cooking loss and emulsion stability of samples decreased upon increasing red yeast rice powder contents. Samples containing 2 and 3% red yeast rice powder showed lower viscosity than others. Sensory evaluation of samples increased with increas in red yeast rice powder content. Thus, red yeast rice powder improved the physicochemical and sensory properties of emulsion-type sausage.

The Literary Investigation On Types and Cooking Method of Bap (Boiled Rice) During Joseon Dynasty($1400's{\sim}1900's$) (조선시대 밥류의 종류와 조리방법에 대한 문헌적 고찰(1400년대${\sim}1900$년대까지))

  • Bok, Hye-Ja
    • Journal of the Korean Society of Food Culture
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    • v.22 no.6
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    • pp.721-741
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    • 2007
  • 1. For the types of boiled rice, there were 1 type of bap, 1 type of jebap cooked with glutinous rice, 13 types of boiled rice cooked by mixing grains and nuts such as daemakban, somakban, jobap, cheongryangmiban, jobap, gijangbap, yulmubap, hyeonmibap, boribap and patmulbap as well as patbap, congbap, byeolbap and bambap etc as ogokbap. Also, there were 12 types of bap cooked by mixing herb medicinal ingredients such as cheongjeong, oban, boksungabap, gumeunsaekbap, hwanggukgamchobap, yeongeunbap, okjeongbap, gogumabap, dububap, samssibap, dorajibap, gamjabap, songibap and jukshilbap. There were 7 types bap cooked as unique one bowl dish at the present as bapby mixing fish, meat, shellfish and milk as ingredients are hwangtang, gyejanggukbap, janggukbap, gulbap, kimchibap, chusaban and bibimbap, etc and the types of bap that have been analyzed are 34 total. 2. For the food ingredients used in bap types 23 types of miscellaneous grains, 5 types of nuts and 11 types of meat, 6 types of fish, 35 types of vegetables, 2 types of fruit including pears or peaches were used. Garlic wasn't used perhaps because of it being boiled rice 3. Types of Sap by Cooking Methods. (1) The ssalbap was cooked by first boiling water, putting in rice grains and boiling hard to be cooked as overcooked bap (rice). (2) The japgokbap (boiled cereals) has used buckwheat, barley job's tear, etc to be boiled down by soaking the ones with large grains (beans) first in advance to be boiled down or cooked by crushing into fine pieces. The red bean, etc was boiled down in advance or placed at the bottom of pot by cutting into two pieces while jujube or nut was cut into three pieces to cook the bap by pouring a lot of water and mixing other ingredients. (3) The gukbap (soup boiled rice), etc were cooked by squeezing out the yellow chrysanthemum that has dried chrysanthemum to cook the boiled rice by putting in rice and gukbap, meat or bones, etc were boiled down for a long time and decorated with meat or wild greens by mixing the bap in the meat juice. For gulbap (oyster boiled rice), etc, it was cooked as ingredients were stir fried in advance or washed and put in when the bap was about half cooked. (4) For bibimbap (mixed boiled rice), after the bap was overcooked first with rice, the wild greens were mixed lightly with bap beforehand, then the wild greens, decorations and garnishings were laid above rice and red pepper powder was sprinkled. (5) Namchok leaves, etc were boiled to cook the boiled rice with rice after being cooled while namchok stem and leaves were pounded to make juice and cooked the bap with rice. The peach, lotus root and yams were cut into fine pieces to be put in together when rice was about half done. The bellflower was soaked in water to be boiled down for a long time while potatoes and pine mushrooms, etc were cut into fine pieces to cook the bap (boiled rice) with rice.

Growth and Predictive Model of Wild-type Salmonella spp. on Temperature and Time during Cut and Package Processing in Cold Pork Meats (냉장돈육 가공공정 온도와 시간에서의 Wild-type Salmonella spp.의 성장특성 및 예측모델)

  • Song, Ju Yeon;Kim, Yong Soo;Hong, Chong Hae;Bahk, Gyung Jin
    • Journal of Food Hygiene and Safety
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    • v.28 no.1
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    • pp.7-12
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    • 2013
  • This study presents the influence on growth properties determined using a novel predictive growth model of wild-type Salmonella spp. KSC 101 by variations in the temperature and time during cut packaging in cold, uncooked pork meat. The experiment performed for model development included an arrangement of different temperatures ($0^{\circ}C$, $5^{\circ}C$, $10^{\circ}C$, $15^{\circ}C$, and $20^{\circ}C$) and time durations (0, 1, 2, and 3 hours) that reflect actual pork-cut and packaging processes. No growth was observed at $0^{\circ}C$ and $5^{\circ}C$, whereas some growth was observed at $10^{\circ}C$, $15^{\circ}C$, and $20^{\circ}C$, with a mean increase of only 0.34 log CFU/g. The growth observed at $20^{\circ}C$ was more robust than that observed at $15^{\circ}C$, but the difference was not statistically significant (p > 0.05). However, compared with PMP (Pathogen Modeling Program), the wild-type Salmonella spp. KSC 101 showed a more rapid growth. We used the Gompertz 4 parameter equation as the primary model, and the exponential decay formula as the secondary model. The estimated $R^2$ values were 0.99 or higher. The developed model was evaluated by comparison of the experimental and predictive values, and the values were in agreement with the ${\pm}0.5$ log CFU/g, although the RMSE (Root mean square error) value was 0.103, which indicates a slight overestimation. Therefore, we suggest that the developed predictive growth model would be useful as a tool for evaluating sanitation criteria in pork cut-packaging processes.