• Asian-Australasian Journal of Animal Sciences
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• 제19권10호
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• pp.1484-1489
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• 2006

This study was carried out to investigate the effect of high pressure and the addition of non-meat proteins on the physico-chemical and binding properties of restructured pork. Pressurizations were carried out at up to 200 MPa and non-meat proteins used as a binder were isolated soy protein (ISP), sodium caseinate (SC), whey protein concentrate (WPC) and egg white powder (EWP). The pH values of all treatments were affected by the level of pressure. $L^*$-value of all treatments increased significantly (p<0.05), while both $a^*$-value and $b^*$-value of all treatments showed a significant decrease (p<0.05) with increasing pressure level. Binders could contribute only additive effects on both pH and color of the treatments. It was found that high pressure improved the water binding capacities and binding strength of the treatments. Binders also improved the binding strength of restructured pork. However, SC and WPC had no effect on water binding properties under high pressure. These results indicate that the application of high pressure had more significant effect on restructuring meat than binders.

• 한국식품과학회지
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• 제52권2호
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• pp.167-171
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• 2020

본 논문에서는 식물성 육류 유사식품에 대한 개념과 소비자 인식, 발달과정, 그리고 시장전망에 대해 조사하였다. 전 세계적으로 육류 소비량 및 요구량이 증가함에 따라 지구온난화와 토지부족과 같은 환경적인 문제가 발생하고 있다. 또한, 소비자들의 종교적 또는 개인적 신념에 따른 식생활에 대응해야 하는 상황에서, 기존 육류를 대체할 수 있는 육류 유사식품의 필요성은 꾸준히 증가하고 있다. 육류 유사식품의 원료 중 대표적으로 식물성 단백질원이 있으며 대두단백, 콩, 밀, 목화씨 등이 이용된다, 동양권에서는 전통적으로 섭취되어온 두부, 세이탄, 템페 등이 식물성 육류 유사식품에 해당한다. 서양에서는 1930년대부터 식물성 육류 유사식품을 본격적으로 생산하기 시작하였으며, 최근 다양한 제품들을 출시하고 있다. 육류 유사식품의 경우 식물성 원료의 압출성형 공정에 대한 연구와 제품의 관능적 특성을 향상시키기 위한 연구가 지속적으로 이루어지고 있다. 전통적인 식육생산 시스템의 한계로 인해 식육 유사식품 시장은 지속적으로 성장할 것이라 예측된다. 특히 최근 출시 중인 식물성 육류 유사식품의 경우, 관능적으로 우수하여 소비자들의 관심이 더욱 증가할 것으로 전망한다.

• 한국축산식품학회지
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• 제18권2호
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• pp.149-156
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• 1998

Chicken beef pork meats and isolated soy protein (ISP) were heated at 10$0^{\circ}C$ for 30min and then heat-resistant proteins were fractionated to examine cross-resistant protein from chicken meat but not with beef pork or ISP. Dot blotting using the polyclonal antibody showed that the sen-sitivity for detecting chicken meat was 1$\mu$m and antibody-antigen reaction was dose-dependant. Results of dot blotting analysis to compare the amount of chicken meat present in arket meat products(Kentucky Frank sausage;chicken meat 46.52% and pork 24.92% vs Bulgogi Ham;chicken meat 28.89% and turkey 31.44%)showed that the significant differences between two meat products in terms of chicken meat concentrations. Dose-dependant dot-blotting reaction was also observed in chicken meat samples with various dilution.

• Asian-Australasian Journal of Animal Sciences
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• 제20권8호
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• pp.1285-1291
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• 2007

This research investigated variation in the improvement of the texture of chicken and pork sausages induced by microbial transglutaminase (MTG). The extractability of myofibrillar proteins from these sausages as well as the ${\varepsilon}-({\gamma}-glutamyl)$lysine (G-L)　content were also investigated. MTG treatment of sausages significantly increased the breaking strength values in both meat types, especially for samples incubated at $40^{\circ}C$. However, values of the breaking strength in both meat types were increased differently. The variation in protein extractability of samples incubated at $40^{\circ}C$ for both meat types could lead to some consideration of the mechanisms and the high accessions of myosin heavy chain (MHC) to MTG. SDS-PAGE analysis showed significant changes in the density of the bands after adding MTG, especially for the pork samples in which the bands disappeared totally. The G-L content in the presence of MTG was double that in control samples of both meat types. This study suggests that the binding ability of myofibrillar proteins with MTG is strong. This leads us to suggest that MTG functions positively with different improvements in the texture of chicken and pork products that are treated mechanically, such as sausages. Variability in gel improvement level between chicken and pork sausages was observed; this resulted from the variation in meat proteins in response to MTG, as well as to the original glutamyl and lysine content.

• 한국축산식품학회지
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• 제39권6호
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• pp.1008-1014
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• 2019

This study investigated the effects of pressure-mediated protein changes on the ice nucleation temperature of pork loins. To variate chemical state of meat proteins, pork loin was pressurized at varying pressure levels (100-500 MPa) for 3 min, and moisture content, expressible moisture (EM) and differential scanning calorimetry (DSC) were analyzed. Although, all treatments showed similar moisture content, EM and degree of protein unfolding of pork loin showed different features as of 300 MPa. At moderate pressure treatments (100-200 MPa), all protein fractions were detected in DSC experiments, and pork loin had lower EM than control (p<0.05). Meanwhile, myosin and actin of pork loin treated at greater than 300 MPa were completely unfolded, and the treatments showed high EM compared to control (p<0.05). Unfolding of meat proteins was a factor suppressing ice nucleation, and the ice nucleation temperature tended to decrease with increasing applied pressure level. The ice nucleation characteristics of pressurized pork loin exhibited a potential application in freezing storage of pressurized meat with less tissue damage comparing to freeze fresh meat, and further exploration regarding the quality change after freezing of fresh and pressurized meat was warranted.

• 한국축산식품학회지
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• 제44권1호
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• pp.132-145
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• 2024

Sarcopenia, the age-related muscle atrophy, is a serious concern as it is associated with frailty, reduced physical functions, and increased mortality risk. Protein supplementation is essential for preserving muscle mass, and horse meat can be an excellent source of proteins. Since sarcopenia occurs under conditions of oxidative stress, this study aimed to investigate the potential anti-muscle atrophy effect of horse meat hydrolysate using C2C12 cells. A horse meat hydrolysate less than 3 kDa (A4<3kDa) significantly increased the viability of C2C12 myoblasts against H₂O₂-induced cytotoxicity. Exposure of C2C12 myoblasts to lipopolysaccharide led to an elevation of cellular reactive oxygen species levels and mRNA expression of proinflammatory cytokines, including tumor necrosis factor-α and interleukin 6, and these effects were attenuated by A4<3kDa treatment. Additionally, A4<3kDa activated protein synthesis-related proteins through the protein kinase B/mechanistic target of rapamycin pathway, while decreasing the expression of activity and degradation-related proteins, such as Forkhead box O3, muscle RING finger protein-1, and Atrogin-1 in dexamethasone-treated C2C12 myotubes. Therefore, the natural material A4<3kDa has the potential of protecting against muscle atrophy, while further in vivo study is needed.

• 한국식품영양학회지
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• 제11권5호
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• pp.576-579
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• 1998

Water extract of mulberry tree barks(Morus alba Linne) was studied for its proteolytic activity. Protein content of the extract was 1.12mg/ml and its specific activity was 5.14U/ml. The enzyme was active on various proteins : the relative acitities were 100 for casein, 63 for albumin, 58 for collagen, 45 for hemoglobin and 36 gelatin, respectively. There suggested that the ability of the enzyme to hydrolyze meat was relatively high since those are major meat proteins. Optimum pH and temperature for proteolytic activity were : pH 6.0 and 6$0^{\circ}C$. And the enzyme was stable at the pH range of 6.0 to 7.0 and temperature between 50 and 8$0^{\circ}C$. Apparent proteolytic activities could support some scientific grounds of traditional application of mulberry tree barks to home cooking for meat tenderization.

• Journal of Animal Science and Technology
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• 제62권2호
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• pp.111-120
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• 2020

The definition of meat analog refers to the replacement of the main ingredient with other than meat. It also called a meat substitute, meat alternatives, fake or mock meat, and imitation meat. The increased importance of meat analog in the current trend is due to the health awareness among consumers in their diet and for a better future environment. The factors that lead to this shift is due to low fat and calorie foods intake, flexitarians, animal disease, natural resources depletion, and to reduce greenhouse gas emission. Currently, available marketed meat analog products are plant-based meat in which the quality (i.e., texture and taste) are similar to the conventional meat. The ingredients used are mainly soy proteins with novel ingredients added, such as mycoprotein and soy leghemoglobin. However, plant-based meat is sold primarily in Western countries. Asian countries also will become a potential market in the near future due to growing interest in this product. With the current advance technology, lab-grown meat with no livestock raising or known as cultured meat will be expected to boost the food market in the future. Also, insect-based products will be promising to be the next protein resource for human food. Nevertheless, other than acceptability, cost-effective, reliable production, and consistent quality towards those products, product safety is the top priority. Therefore, the regulatory frameworks need to be developed alongside.

• 한국식품위생안전성학회지
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• 제6권1호
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• pp.67-72
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• 1991

어묵에 grapefruit 종자 추출물의 농도별로, 침지 처리한 후 저장기간에 따른 물리, 화학적 변화를 검토하여 다음과 같은 결과를 얻었다. 1. 저장 기간 중 어묵의 조단백함량 변화는 대조구에 비하여 GFSE 의 처리 농도가 높을수록 더 작았다. 2. Texture 는 저장기간이 경과함에 따라 감소하는 경향을 나타내었으나 GFSE 용액 처리구에서는 감소율이 낮았다. 3 어묵 단백질의 SDS-PAGE pattern 변화는 저장기간이 경과함에 따라 분자량, 30,000-32,000의 protein은 점차 가수분해되어 소멸되는 현상을 보였으며 특히 대조군의 경우 2일 경과 후부터 급격한 분해라 이루어져 단백질 major band의 손실율이 크게 증가한 것으로 나타난 반면, GFSE처리 시험구의 경우 4일 경과시에야 비로소 뚜렷한 손실반응을 보여 GFSE처리에 의하여 어묵단백질의 변패 발생 시기를 상당 기간 연장함을 확인할 수 있다.

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2002년도 제30차 추계 학술대회
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• pp.166-166
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• 2002