• East Asian mathematical journal
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• 제29권3호
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• pp.315-326
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• 2013

In this paper, a new monotonicity, $M({\cdot},{\cdot})$-monotonicity, is introduced in Banach spaces, and the resolvent operator of an $M({\cdot},{\cdot})$-monotone operator is proved to be single valued and Lipschitz continuous. By using the resolvent operator technique associated with $M({\cdot},{\cdot})$-monotone operators, we construct a proximal point algorithm for solving a class of variational inclusions. And we prove the convergence of the sequences generated by the proximal point algorithms in Banach spaces. The results in this paper extend and improve some known results in the literature.

• 한국CDE학회논문집
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• 제4권1호
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• pp.43-51
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• 1999

Producing the relevant information (features) from the CAD models of CAM, called feature recognition or extraction, is the essential stage for the integration of CAD and CAM. Most feature recognition methods, however, have problems in the recognition of intersecting features because they do not handle the intersection geometry properly. In this paper, we propose a machining feature recognition algorithm, which has a solid model consisting of orthogonal primitives as input. The algorithm calculates candidate features and constitutes the Intersection Geometry Matrix which is necessary to represent the spatial relation of candidate features. Finally, it recognizes machining features from the proposed candidate features dividing and growing systems using half space and Boolean operation. The algorithm has the following characteristics: Though the geometry of part is complex due to the intersections of design primitives, it can recognize the necessary machining features. In addition, it creates the Maximal Feature Volumes independent of the machining sequences at the feature recognition stage so that it can easily accommodate the change of decision criteria of machining orders.

• 한국통신학회논문지
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• 제22권7호
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• pp.1590-1598
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• 1997

The paper first introduces the new concept of potential diversity and signal decomposability, which establish a foundaton to generalize the existing concepts of path and frequency diversities. Then it presents a new DS/CDMA system called chip-spreading OCDM system, which is an embodiment of the petential diversity concept that combines the path diversity of the DS/CDMA system and the frequency diversity of the OFDM/CDMA system. In the chip-spreading OCDM system the chip sequences in each symbol interval are first converted into aralled streams, which then simultaneously modulate different orthogonal Walsh basis functions. In the receiver, the received signal is matched to each extended basis-function which is the union of the transmitter basis-functions and their delayed replicas, and the matched-filtered chip samples are combined together after individual channel compensation. The conventional DS/CDMA system using the maximal ratio combining. In addition, it effectively resolves the high PAR and high sensitivity to frequency offset problems which are critical in multi-carrier systems.

• East Asian mathematical journal
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• 제29권5호
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• pp.529-536
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• 2013

In this paper, we find the values and the number of occurrences of each value of the cross-correlation function $C_d({\tau})$ when $d=\frac{2^{k-1}}{2^s-1}(2^{k(i+1)}-2^{ki}+2^{s+1}-2^k-1)$, where n = 2k, s is an integer such that 2s divides k, and i is odd.

• 한국양식학회지
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• 제10권4호
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• pp.409-415
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• 1997

The carp $\beta$-actin regulatory sequences and RSV/LTR promoter were tested whether they are functinal to express linked structure gene (chloramphenicol acetyltransferas, CAT) in olive flounder (Paralichthys olivaceus) by determining the patterns of gene expression following intramuscular in vivo direct injection. The injection experiments with various concentrations of both pRSVCAT and pFV4CAT clearly revealed the effectiveness of DNA dosage on expression of CAT. The increase of CAT activity was linear in both plasmids, and maximal CAT activity was obtained with 100 ug of pFV4CAT injection. The amounts of CAT expression with pFV4CAT-injected fist were higher than those with pRSVCAT-injected fish. CAT activity was readily detectable as early as one day after injection, slightly increased at day 2, and declined over time. Most amount of DNA intramuscularly injected into olive flounder muscles persisted extrachromosomally without showing any integrated or replicated form in vivo.

• 한국운동역학회지
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• 제18권2호
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• pp.95-104
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• 2008

본 연구는 남자 중학교 초보피험자들을 대상으로 태권도 돌려차기 동작의 숙련정도에 따른 운동학적 협응과 제어과정을 살펴보는 데 목적을 두었다. 이용된 변인은 최대합성직선속도와 각도 대 각도 도면이었다. 분석결과, 연습후기로 갈수록 인접한 분절간의 운동량 전이가 잘 이루어져 각 분절의 최대합성직선속도가 증가하였으며 무릎관절 최대굴곡 시까지는 엉덩관절과 무릎관절이 동형동조 협응형태로 변해갔으며, 최대굴곡 후 타격 시까지는 모든 숙련 단계에 있어서 이형동조 협응패턴을 나타내었다. 발목관절은 무릎관절 최대굴곡 시까지 배측굴곡 상태에서 저측굴곡으로 변했으며, 최대굴곡 후 타격시점까지는 발목관절은 고정시키고, 무릎관절은 신전시키는 자유도 고정 제어기전을 나타내었다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제9권1호
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• pp.169-189
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• 2015

Detection of anomalous events from video streams is a challenging problem in many video surveillance applications. One such application that has received significant attention from the computer vision community is traffic video surveillance. In this paper, a Lossy Count based Sequential Temporal Pattern mining approach (LC-STP) is proposed for detecting spatio-temporal abnormal events (such as a traffic violation at junction) from sequences of video streams. The proposed approach relies mainly on spatial abstractions of each object, mining frequent temporal patterns in a sequence of video frames to form a regular temporal pattern. In order to detect each object in every frame, the input video is first pre-processed by applying Gaussian Mixture Models. After the detection of foreground objects, the tracking is carried out using block motion estimation by the three-step search method. The primitive events of the object are represented by assigning spatial and temporal symbols corresponding to their location and time information. These primitive events are analyzed to form a temporal pattern in a sequence of video frames, representing temporal relation between various object's primitive events. This is repeated for each window of sequences, and the support for temporal sequence is obtained based on LC-STP to discover regular patterns of normal events. Events deviating from these patterns are identified as anomalies. Unlike the traditional frequent item set mining methods, the proposed method generates maximal frequent patterns without candidate generation. Furthermore, experimental results show that the proposed method performs well and can detect video anomalies in real traffic video data.

• Journal of Life Science
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• 제9권1호
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• pp.26-34
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• 1999

A thermostable pullulanase has been isolated and purified from Thermus caldophilus GK-24 to a homogeneity by gel-filtration and ion-exchange chromatography. The specific activity of the purified enzyme was 431-fold increase from the crude culture broth with a recovery of 11.4%. The purified enzyme showed $M_{r}$ of 65 kDa on denaturated and natural conditions. The pI of the enzyme was 6.1 and Schiff staining was negative, suggesting that the enzyme is not a glycoprotein. The enzyme was most active at pH 5.5. The activity was maximal at $75^{\cire}C$ and stable up to $95^{\cire}C$ for 30 min at pH 5.5. The enzyme was stable to incubation from pH 3.5 to pH 8.0 at $4^{\cire}C$ for 24hr. The presence of pullulan protected the enzyme from heat inactivation, the extent depending upon the substrate concentration. The activity of the enzyme was simulated by $Mn^{2+}$ ion, }$Ni^{2+}$, $Ca^{2+}$, $Co^{2+}$ ions. The enzyme hydrolyzed the ${\alpha}$-1,6-linkages of amylopectin, glycogens, ${\alpha}$, ${\beta}$-limited dextrin, and pullulan. The enzyme caused the complete hydrolysis of pullulan to maltotriose and the activity was inhibited by $\alpha$, $\beta$, or $\gamma$-cyclodextrins. The $NH_{2}$-terminal amino acid sequence [(Ala-Pro-Gln-(Asp of Tyr)-Asn-Leu-Leu-Xaa-ILe-Gly-Ala(Ser)] was compared with known sequences of various sources and that was compared with known sequences of various sources and that was different from those of bacterial and plant enzymes, suggesting that the enzymes are structurally different.

• Journal of Microbiology and Biotechnology
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• 제20권10호
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• pp.1403-1414
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• 2010

Aspergillus awamori BTMFW032, isolated from sea water, produced tannase as an extracellular enzyme under submerged culture conditions. Enzymes with a specific activity of 2,761.89 IU/mg protein, a final yield of 0.51%, and a purification fold of 6.32 were obtained after purification through to homogeneity, by ultrafiltration and gel filtration. SDS-PAGE analyses, under nonreducing and reducing conditions, yielded a single band of 230 kDa and 37.8 kDa, respectively, indicating the presence of six identical monomers. A pI of 4.4 and a carbohydrate content of 8.02% were observed in the enzyme. The optimal temperature was found to be $30^{\circ}C$, although the enzyme was active in the range of $5-80^{\circ}C$. Two pH optima, pH 2 and pH 8, were recorded, although the enzyme was instable at a pH of 8, but stable at a pH of 2.0 for 24 h. Methylgallate recorded maximal affinity, and $K_m$ and $V_{max}$ were recorded at $1.9{\times}10^{-3}$M and 830 ${\mu}Mol$/min, respectively. The impacts of a number of metal salts, solvents, surfactants, and other typical enzyme inhibitors on tannase activity were determined in order to establish the novel characteristics of the enzyme. The gene encoding tannase, isolated from A. awamori, was found to be 1.232 kb, and nucleic acid sequence analysis revealed an open reading frame consisting of 1,122 bp (374 amino acids) of one stretch in the -1 strand. In silico analyses of gene sequences, and a comparison with reported sequences of other species of Aspergillus, indicate that the acidophilic tannase from marine A. awamori differs from that of other reported species.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.420-422
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• 2005

TMC (Tautomycetin) is a liner polyketide immunosuppressive antifungal compound produced by Streptomyces spp. Inhibition of T cell proliferation with TMC was observed highly efficient at 100-fold lower than those needed to achieve maximal inhibition with cyclosporin A. To elucidate the biosynthetic pathway of TMC, a genomic DNA library was constructed using a E. coil-Streptomyces shuttle cosmid vector, pOJ446. The DNA libraries were screened by colony blot hybridization using several polyketide ${\beta}-ketosynthase$ (KS) probes amplified from TMC-producing Streptomyces genomic DNA using polymerase chain reaction (PCR), of which the degenerate primers were designed based on the highly conserved sequences present in KS domains of various type I polyketide synthase genes in Streptomyces species. This library construction and screening approach led to the isolation of several positive cosmid clones representing type I polyketide biosynthetic gene clusters. In addition, a Streptomyces regulatory gene called afsR2 (a global regulatory gene stimulating antibiotic production in both S. coelicolor and S. lividans) was successfully integrated into the TMC-producing Streptomyces chromosome via E. coil-Streptomyces heterologous conjugation mehtod. The more detailed results of production improvement and genetic characterization of TMC-producing Streptomyces spp. will be discussed.