• Food Science and Biotechnology
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• 제17권3호
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• pp.587-593
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• 2008

Microbial exopolysaccharide (EPS) is a biothickener that can be added to a wide variety of food products, where it serves as a viscosifying, stabilizing, emulsifying, and gelling agent. The objective of this study was to investigate the optimum conditions of pH, incubation temperature, and whey protein concentration (WPC) for EPS production by Lactobacillus rhamnosus ATCC 9595. We found that maximal EPS production was achieved at a pH of 5.5 and temperature of $37^{\circ}C$. At the same fermentation conditions, EPS production was affected by the addition of L. rhamnosus GG (a weak-EPS producer). After growth for 24 hr, total EPS production was $583{\pm}15.4mg/L$ in the single culture system, and $865{\pm}22.6\;mg/L$ in the co-culture system with L. rhamnosus GG. Based on the presence of WPC, EPS production dramatically increased from $583{\pm}15.4$ (under no WPC supplementation) to $1,011{\pm}14.7\;mg/L$ (under supplementation with 1.0% WPC). These results suggest that WPC supplementation and the co-culture systems coupled with small portions of weak-EPS producing strain can play an important role in the enhancement of EPS production.

• 한국전문물리치료학회지
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• 제27권2호
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• pp.118-125
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• 2020

Background: Lateral epicondylitis (LE) is the most common chronic musculoskeletal pain condition of the upper extremities. LE is often related to forceful grip activities that require isometric contraction of the wrist extensors. A previous study evaluated the effect of the diamond taping technique on grip strength and pain; however, there has been no report on the change in the electromyography (EMG) findings of wrist extensors. Objects: The aim of this study was to investigate the effect of diamond taping technique, using a rigid tape, on the EMG activities of the extensor carpi radialis (ECR) during grip activities. Methods: Twenty-four healthy subjects (mean age = 21.50 ± 2.76 years) volunteered to participate in this study. The subjects were instructed to perform forceful grip activities with and without diamond-type taping on the origin area of the ECR. Grip strength tests were performed at 100%, 75%, 50%, and 25% for maximal isometric contraction force. EMG data were collected from the ECR. Repeated measure analysis of variance was used to analyze the effect of grip force and taping (with and without). Statistical significance levels were set at α = 0.05. Comparison of the results with and without taping at different grip force were analyzed using independent t-test. Statistical significance levels were set at α = 0.01. Results: Statistically significant association was observed between the taping application and forceful grip activity as revealed by the EMG data of the ECR (p < 0.05). EMG of the ECR significantly reduced for all muscle strength levels (p < 0.01) after taping. Conclusion: This study shows an impressive effect of the diamond taping technique, using rigid tape, on wrist extensors during grip activities. Decreasing muscle activity via this taping approach could be utilized to enhance pain-free grip force and reduce pain in patients with LE. Our study suggested that this taping technique could be considered as an effective management strategy of LE.

• Advances in Traditional Medicine
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• 제10권1호
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• pp.7-12
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• 2010

Typha orientalis' stem (TOS) is traditionally used as an herbal medicine for difficulty in urination, galactophoritis purulenta, whooping cough, and allergic dermatitis. However, its effect in experimental models remains unknown. Here, we report the effect of TOS on the phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187-induced inflammatory cytokine production and extracellular signal-regulated kinase (ERK) activation in the human mast cell line, HMC-1. TOS inhibited PMA plus A23187-induced cytokines such as tumor necrosis factor-alpha (TNF-$\alpha$) and interleukin (IL)-6. Maximal inhibition rate of TNF-$\alpha$ and IL-6 production by TOS (1 mg/ml) was about 44.02%, and 45.20%, respectively (P < 0.05). In addition, TOS inhibited the expression of TNF-$\alpha$ and IL-6 mRNA under the same condition. Moreover, TOS partially blocked PMA plus A23187-induced ERK phosphorylation. These results suggested TOS could inhibit the cytokine production through blocking of ERK activity.

• 한국식품영양과학회지
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• 제31권5호
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• pp.754-759
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• 2002

공업적으로 광범위하게 이용되고 있는 protease 중 중성 영역에서 최적의 활성을 갖는 중성 protease에 대한 연구의 일환으로, 토양으로부터 protease의 생성능이 우수한 10여 균주를 순수 분리하였으며, 이들 중에서 protease의 활성 이 가장 우수한 PANH765를 최종 선별하였다. 이 균주의 형태학적, 배양학적, 생리학적 및 생화학적 특성을 조사하여 Bergey의 세균 분류서의 색인에 따라 체계적으로 분류한 결과, Bacilius subtilis 또는 그 유연균으로 동정되어 분리균을 Bacilius .Subtilis PA-NH765로 동정하였다. B. subtilis PANH-765 균주에 의한 protease의 최적 배지 조성은 2.0% glucose, 1.0% yeast extract, 0.2% ammonium nitrate, 0.02% ferrous sulfate 및 0.02% di-potassium hydrogen phosphate이었으며, 최적 생산 조건은 배양온도 ,3$0^{\circ}C$, pH 7.0, 진탕 속도 150 rpm이었다. 이 조건에서 초기 균체량을5%(w/v)접종하여 배양하였을 때 시간에 따른 효소의 최대 활성은 배양 ,36시간일 때 6.34 U이었다.

• KSBB Journal
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• 제14권2호
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• pp.241-247
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• 1999

디프테리아는 Corynebacterium diphtheriae에 의해 발생되는 호흡기 질병으로 C. diphtheriae의 exo-toxin을 불활성화 시킨 toxoid 백신을 사용하여 예방해 왔다. 현재까지 국내에서는 정치배양 방법으로 디프테리아 toxin을 생산해 왔기 때문에 생산성과 품질에 한계가 있었으며, 이를 극복하기 위해 발효조를 이용한 발효조건 최적화에 대한 연구를 수행하였다. 디프테리아 toxin을 보다 효율적을 얻기 위한 배지로서 beef antigen이 함유되어 있지 않은 casein 유래의 NZ-Case를 선택하였다. 발효조에 의한 toxin 생성은 세포성장과 함께 증가하는 growh-associated form으로 나타났다. 최대의 세포성장과 toxin 생성은 초기 pH가 7.0인 배지에서 0.22vvm의 통기와 400rpm의 교반조건에서 얻을 수 있었다. 또한 최대의 toxin 생성을 위한 최적의 iron 이온의 농도는 0.3mg/L 이었으며, morgamcphospale의 첨가에 의한 calcium-phosphate 침전이 배지 내에 iron 이온의 농도조절을 위하여 요구되었다. 면역원성을 확인하기 위한 역가시험 결과 발효조 배양에서 얻은 toxoid는 4단위에서 모든 guinea-pigs가 생존하여 2단위인 정치배양 toxin에비해 월등히 우수한 것으로 판단되었다. 결국 발효조 배양으로 toxin을 생산할 경우 부작용이 적고 수율과 생산성 및 면역원성 이 우수한 toxid의 생산이 가능하였다.

• ALGAE
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• 제19권2호
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• pp.145-148
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• 2004

As a result of the 2-year monthly monitoring of the phytoplankton community at 3 stations in Mankyeong Estuary, Korea, we learned that cyan bacterial species of the genus Anabaena occurred at most sampling points with huge salinity differences (0.1-32.5 psu). We isolated several clones of Anabaena spp. from the monitoring stations, and screen out two euryhaline and nitrogen-fixing Anabaena clones, CB-MAL21 and CB-MAL22. The two clones were grown under various environmental gradients such as temperature (20, 30, 35 and 40$^{\circ}C$), salinity (0, 2, 5, 15 and 30psu), and $PO_4^{3-}$-P concentration (0, 1.6, 8.0, 40 and 200 ${\mu}M$M). Growth of CB-MAL21 and CB-MAL22 was measured by daily monitoring of chlorophyll fluorescence from each experimental culture for more than three serial transfers. Both the two experimental clones did not grow at 0psu. Maximal growth rates of the two clones were markedly reduced at lower $PO_4^{3-}$-P concentrations showing negligible growth at 0 and 1.6 ${\mu}M$M. However, growth of CB-MAL21 was not affected by low $NO_3^--$ concentration in culture media, showing the nitrogen-fixing ability. Maximum biomass yields of the two clones decreased dramatically at 35 and 40$^{\circ}C$. Optimal growth conditions for the two experimental clones were determined to be 20-30$^{\circ}C$, 40 ${\mu}M$M $PO_4^{3-}$-P, and wide salinity range from 5.0 to over 30psu. Best growth of CB-MAL21 was shown at (20$^{\circ}C$-15psu), which is less saline and cooler condition than those (i.e., 30$^{\circ}C$-30psu) for the best growth of CB-MAL22. The euryhaline and nitrogen-fixing CB-MAL21 strain thus can be a candidate laboratory culture for the future cyan bacterial marine biotechnology in temperate coastal waters.

• The Journal of Korean Physical Therapy
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• 제13권2호
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• pp.381-397
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• 2001

In the patients with hemiplegia caused by stroke and TBI. postural sway is increased and open displaced laterally over the non-affected leg, reflecting asymmetry in weight bearing on lower extremities during standing balance. Recovery of symmetric weight bearing and postural stability is an important aim in physical therapy. Plastic AFO has been used for hemiplegic patients in order to help their abnormal walking patterns. Past studies have mainly focused on the AFO influences on hemiplegic walking patterns without balance function approaches. The purpose of this study was to identify the immediate effects of plastic AFO and shoes on the static balance in hemiplegic patients. The scale for static balance were weight bearing on affected leg(%), sway area(mm2), sway path(mm), maximal sway velocity(mm/s), anteroposterior sway angle($^{\circ}$ ), and lateral angle($^{\circ}$ ). Seventeen hemiplegic patients participated in this study: 13 men and 4 women, with an average age of 50.18 years. Static balance was measured using BPM(balance performance monitor; dataprint software version 5.3) under four standing condition namely bare-foot standing. standing in shoes, standing with AFO, and standing in shoes with AFO. In order to assure the statistical significance of the results, an one-way ANOVA, the independent t-test. and a pearson's correlation were applied at the .05 level of significant. The results of this study were as follows: 1) There were statistically significant differences in weight bearing(%) on the static balance between affected leg and non-affected leg(p<.01). 2) There were statistically significant differences in sway reverse frequence(Hz) in standing with AFO between affected leg and non-affected leg(p<.05). 3) Sway area(mm2) on standing in shoes with AFO was lower than bare-foot standing(p<.05), Lateral sway angle($^{\circ}$ ) on standing in shoes with AFO was lower than bare-foot standing and standing in shoes(p<.05). 4) Weight bearing in affected leg was not significantly correlated with postural sway.

• 한국균학회지
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• 제30권1호
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• pp.11-17
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• 2002

공시균주인 EFCC C-251, EFCC C-252, EFCC C-1092 균주 중 현미(60 g)와 누에 번데기(30 g)를 혼합한 배지에서 EFCC C-252 균주가 자실체가 잘 형성되어 이 균주를 가지고 본 연구를 수행하였다. 자실체 형성 최적온도는 $25^{\circ}C$이었으며 광은 형광등으로 조명도는 300 lux이었다. 기본배지에 lactose, ammonium sulfate와 $KH_2PO_4$를 첨가한 배지에서 균사의 생육은 좋았으나 자실체 형성은 기본배지에 현미(20 g)를 첨가한 배지에서 좋았다. 현미만으로는 70 g를 넣은 배지와 누에 번데기만은 80 g를 넣은 배지에서 에서 자실체의 형성이 좋았다. 현미와 누에번데기를 같이 넣었을 때는 현미 60 g에 누에번데기 40 g에서 다른 비율배지에 비해 자실체 형성이 좋았다. 관수는 2일 간격으로 한 처리구가 좋았으며 액체종균의 접종량은 $5{\sim}25\;ml$까지는 자실체 생육이 비슷함을 보이다가 접종량이 증가할수록 자실체 생육이 저조하였다.

• 대한기계학회논문집B
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• 제31권9호
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• pp.790-798
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• 2007

An experimental study was performed to investigate the effects of mixing quality, inlet pressure, nozzle diameter on CO, NO emission and radiation characteristics in porous ceramic fiber radiant burners. Observations of combustion characteristics occurring inside the burner system which was insulated fiber mat, were investigated by measuring emission and radiation characteristics. Combustion was achieved at the firing rate of $88{\sim}99\;kcal/hr$, inlet pressure of $100{\sim}250mmH_2O$. The fiber burner exhibit significant both spectral intensity peaks in the bands at $2.5{\mu}m\;and\;4.0{\mu}m$ relatively. There is a small difference in the variable mixing tube. However spectral intensity increased with the firing rate. CO emissions were found to be strongly dependent on the operating conditions. There was a tendency that CO concentration increased as the firing rate increases. the reason for rise of CO concentration is that is becomes it the relatively rich condition. Relatively low NO emission was observed for the whole operating range. The NO concentration is maximal at the firing rate of approximately 2850 kcal/hr and an air ratio of about 1.

• Applied Biological Chemistry
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• 제37권6호
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• pp.427-432
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• 1994

광합성세균인 Rhodocyclus gelatinosus KUP-74의 휴지균체에 의한 균체외 ${\delta}-aminolevulinate$(ALA) 생산 효율성에 관하여 검토하였다. 균체외 ALA의 최대 생산치를 위하여 1 ml ALA 생산계는 1.05 mg의 휴지균체로 6시간의 배양을 필요로 하였다. 또한, 이 조건에서 ALA 생산의 유도물질인 levulinate나 L-glutamate 등은 균체외 ALA 생산에 있어서 비교적 낮은 효과를 나타내었다. 반면에, ALA 생산계의 부피와 이에 적합한 균체밀도가 효과적인 ALA 생산을 위하여 중요한 요인으로 나타났다. 휴지균체의 안정한 ALA 생산유지를 위한 수명은 휴지균체를 고정화 시키므로써 크게 연장되었다.