The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Investigation of the Condition of Fruiting Body Formation by Cordyceps scarabaeicola

풍뎅이동충하초(Cordyceps scarabaeicola)의 자실체 형성 조건

  • Lee, Jae-Keun (Department of Environmental Biology, Kangwon National University) ;
  • Sung, Jae-Mo (Department of Environmental Biology, Kangwon National University) ;
  • Park, Young-Joon (Chiaksan National Park Office)
  • 이재근 (강원대학교 자원생물환경학부) ;
  • 성재모 (강원대학교 자원생물환경학부) ;
  • 박영준 (국립공원관리공단 치악산관리사무소)
  • Published : 2002.04.30
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Abstract

This experiment was carried out to study formation of fruitbody with Cordyceps scarabaeicola (EFCC C-252) isolate. This isolate was the one of best fruitbody formation on brown rice 60 g plus 30 g silkworm pupa media among EFCC C-251, EFCC C-252, EFCC C-1092 from EFCC (Entomopathogenic Fungal Culture Collection) of Kangwon National University. Fruiting body was formed only isolate EFCC C-252 among tested isolates on the medium of brown rice (60 g) and silkworm pupae (30 g). The optimal temperature and light for fruiting body formation were $25^{\circ}C$ and fluorescent light (300 lux). The maximal fruiting body formation was observed at 70 g of brown rice and 80 g of silkworm pupa medium which was treated separately. Fruiting body was formed maximally by 2 days interval of irrigation.

공시균주인 EFCC C-251, EFCC C-252, EFCC C-1092 균주 중 현미(60 g)와 누에 번데기(30 g)를 혼합한 배지에서 EFCC C-252 균주가 자실체가 잘 형성되어 이 균주를 가지고 본 연구를 수행하였다. 자실체 형성 최적온도는 $25^{\circ}C$이었으며 광은 형광등으로 조명도는 300 lux이었다. 기본배지에 lactose, ammonium sulfate와 $KH_2PO_4$를 첨가한 배지에서 균사의 생육은 좋았으나 자실체 형성은 기본배지에 현미(20 g)를 첨가한 배지에서 좋았다. 현미만으로는 70 g를 넣은 배지와 누에 번데기만은 80 g를 넣은 배지에서 에서 자실체의 형성이 좋았다. 현미와 누에번데기를 같이 넣었을 때는 현미 60 g에 누에번데기 40 g에서 다른 비율배지에 비해 자실체 형성이 좋았다. 관수는 2일 간격으로 한 처리구가 좋았으며 액체종균의 접종량은 $5{\sim}25\;ml$까지는 자실체 생육이 비슷함을 보이다가 접종량이 증가할수록 자실체 생육이 저조하였다.

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