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http://dx.doi.org/10.3746/jkfn.2002.31.5.754

Isolation and Characterization of Pretense Producing Bacteria from Soil  

김관필 (경북대학교 식품공학과)
이창호 (경북대학교 식품공학과)
우철주 (경북대학교 식품공학과)
김남형 (건국대학교 응용생물화학과)
배동호 (건국대학교 응용생물화학과)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.31, no.5, 2002 , pp. 754-759 More about this Journal
Abstract
In order to develope a new pretense applicable to industries, a bacterium which produces a remarkable amount of extracellular pretense were isolated from soil. About 10 bacterial strains producing pretense were isolated from samples of soil, and strain PANH765 showed the highest activity of pretense production among them. The strain was identified as Bacillus subtilis according to the Bergey's Manual of Systematic Bacteriology based on its morphological, cultural and physiological characteristics. B. subtilis PANH765 showed the maximal production of pro-tease in the medium containing 2.0% glucose, 1.0% yeast extract, 0.2% ammonium nitrate, 0.02% ferrous sulfate and 0.02% dipotassium hydrogen phosphate. Under the optimal condition with temperature of 3$0^{\circ}C$, initial pH of 7.0 and shaking speed of 150 rpm, the pretense production reached a maximum level with 36 hr cultivation (6.34 U).
Keywords
extracellular protease; Bacillus subtilis PANH765; optimal conditions;
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