• Journal of the Korean Wood Science and Technology
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• 제25권2호
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• pp.117-126
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• 1997

In fast grown softwood, there are very large changes in material properties going outward from the pith to bark such as anatomical, physical and mechanical characteristics. Some of variations in anatomical properties with annual ring were then examined from Pinus koraiensis, Larix leptolepis, and Chamaecyparis obtusa, which are major softwoods of plantation in Korea. The large variations of annual ring width during young age of tree tended to stabilize after 25year through the transitional period in 17~23year. The ring density was 1.5~2.4 in 1~10year period, and 3.5~6.3 in 30~35year period, in which juvenile and mature wood were certainly assumed to be formed, respectively. Variations of tracheid length showed functional relationships with annual rings as logarithm. Demarcation between juvenile wood and mature wood could be 16~19year, which was determined from increase rate of tracheid length of 0.2%. Cell wall thickness increased with increase of annual ring even though large variations were observed as well. Variations of cell wall thickness within species were pronounced in latewood than earlywood. The increase of cell wall thickness from juvenile wood to mature wood was predominant in Larix leptolepis as 2.0times, and least in Chamaecyparis obtusa as 1.1 times. Cell diameters showed trends of increase during young age of 1~15year, and consistent afterward. The variations of cell diameter between radial and tangential direction were greater in latewood, and most pronounced in Chamaecyparis obtusa.

• 한국수산과학회지
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• 제24권5호
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• pp.327-339
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• 1991

줄새우아재비, Palaemon serrifer의 뇌와 흉부신경절에 분포하는 신경분비세포와 생식소발달과의 관련 기능을 알아보고자 생식소발달의 조직학적 변화를 조사하여 생식년주기를 밝히고, 뇌와 흉부신경절에 분포하는 신경분비세포를 분류하여 분비활성변화를 조사하였으며, 생식소발달에 따른 이들 분비세포들의 활성변화를 연구하였다. 1. 줄새우아재비, Palaemon serrifer는 수컷의 경우 1월부터, 암컷은 3월부터 생식소가 성장하기 시작하는 성장기를 거쳐, 성숙기, 완숙 및 산란기, 퇴화 및 휴지기의 연속된 생식연주기를 가지며, 주산란기 는 7-8월이었다. 2. 신경분비세포로서 뇌에서는 A-, B-그리고 E-cell이 흉부신경절에서는 A-, A'- 그리고 B-cell이 구분되었으며 A-와 A'-cell은 크기가 $80-90{\mu}m$로 가장 큰 세포였고 B-cell은 $30-40{\mu}m$의 크기였으며, E-cell은 $10-15{\mu}m$ 크기로 미세한 세포였다. 3. 활성중인 A-와 B-cell은 CHP와 AF에, 그리고 B-cell은 AF에만 양성반응을 나타냈었고, A-cell은 분비과립이 축색으로 이동하는 것 외에 주변방출(peripheral discharge)을 나타냈다. 4. 신경분비세포의 활성변화를 생식소발달상태와 연관하여 볼 때 난소의 성장과 성숙시기에는 E-cell, 배란시기에는 A-cell의 분비활성이 강했고, 정소의 성장시기에는 E-cell, 정자의 변태 및 방정시기에는 A-cell의 강한 분비활성이 관찰되었다.

• Applied Biological Chemistry
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• 제34권1호
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• pp.32-37
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• 1991

감과실이 성숙함에 따라 세포가 비대성장하고 세포간극이 발달하였으며 연시에서는 세포가 분리되어 독립적으로 존재하였다. 또 성숙중에는 원형질내에 small vesicle이 생기고, 완숙감과실에서 middle lamella의 분해현상을 관찰하였다. 연시에서는 세포벽과 middle lamella가 분리 용해되었다.

• Food Science and Biotechnology
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• 제14권6호
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• pp.709-714
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• 2005

Alteration of molecular markers related to apoptosis of in vivo normal system and in vitro cancerous system by soy-isoflavonoid with estrogen was investigated. Down-regulation of Bcl-2 was accompanied by decreased expression of COX-2 (cyclooxygenase-2) in mature female rats treated with soy-isoflavonoid and estrogen. In ovariectomized rat system, Bax was regulated by higher concentration of soy treatment. Bax up-regulation by soy-isoflavonoid genistein treatment was observed in MCF-7 mammary cancer cell system. Estrogen without soy induced similar pattern of Bax expression as soy-isoflavonoid in vivo, but exhibited opposite trend in vitro. These findings suggest soy-isoflavonoid may have potential to induce apoptosis at higher concentrations through up-regulation of Bax or down-regulation of Bcl-2 expressions depending on normal or cancerous state, and physiological status of rats.

• 한국수정란이식학회지
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• 제11권3호
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• pp.233-240
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• 1996

This study was carried out to determine the effect of cumulus cell attachment and various factors on in vitro maturation of pig foflicular oocytes. Oocytes with various configuration of cumulus cell mass were collected ftom ovaries of mature gilts by asperating with syringe equipped with needles of different gauges, follicle size and with or without cumulus cells. They were cultured in TCM-199 mediun containing FGS(fetal calf serum) for 30~48 hours in incubator with air containing 5% $CO_2$ at 38.5$^{\circ}C$. Mter orcein staining at in vitro maturation condition, GV, GVBD, anaphase, telophase and M II were observed. Results are surumarized as follows: 1. Recovery rates were 55.8, 55.5 and 34.4% when the cumulus-compacted oocytes were collected with 18, 21, 26 gauge needles of syringes, respectively. 2. 79% of oocytes with compacted cumulus cells were at GV stage and most of the oocytes with partially denuded and denuded cumulus cells were from GVBD to M- II stages. 3. Percentage of mature oocytes among those which are follicular diameter of 1~2, 3~6 and over 6 mm was 42.6, 53.2 and 60.8%, respectively. 4. Percentage of mature oocytes among those which are compacted, partially denuded and denuded was 60.5, 46.2 and 35.4% respectively. 5. Percentage of mature oocytes in co-cultured with monolayers of cumulus cells was higher (57.1%) than that found with oocytes cultured alone (53.4%).

• KSBB Journal
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• 제27권5호
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• pp.295-300
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• 2012

Matured dendritic cells (DCs) begin migration with their release from the bone marrow (BM) into the blood and subsequent traffic into peripheral lymphoid and non-lymphoid tissues. Throughout this long movement, migrating DCs must apply specialized skills to reach their target destination. Non-invasive in vivo cell-tracking techniques are necessary to advance immune cell-based therapies. In this study, we used a DiD cell-tracking solution for in vivo dendritic cell tracking in naive mice. We tracked DiD (non-invasive fluorescence dye)-labeled mature dendritic cells using the Near Infrared (NIR) imaging system in normal mice. We examined the immunophenotype of DiD-labeled cells compared with non-labelled mature DCs, and obtained time-serial images of NIR-DC trafficking after mouse footpad injection. In conclusion, we confirmed that DiD-labeled DCs migrated into the popliteal lymph node 24 h after the footpad injection. Here, these data suggested that the cell tracking system with the stable fluorescence dye DiD was useful as a cell tracking tool to advance dendritic cell-based immunotherapy.

• 대한바이러스학회지
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• 제28권4호
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• pp.295-302
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• 1998

The RNA genome of poliovirus encodes a long polyprotein precursor and this polyprotein is cleaved proteolytically by viral protease to yield mature proteins. The mature proteins derived from the P1 polyprotein precursor are the component of capsids. To further delineate the process of capsid assembly and encapsidation, in a first attempt, a cell line which expresses the authentic P1 polyprotein was established. CV-1 cells were transfected with the pRCRSVS1P1 plasmid DNA which contains 5'ncr sequences, whole authentic capsid gene of poliovirus and neomycin resistance gene. These cells were treated with G418 for 3 months, and eventually G418 resistant cells were selected and formed colonies. Each colony was picked and grown in the media containing G418. DNA analysis indicated that 1 of 13 neomycin resistant cell lines (R2-18) contains whole poliovirus P1 capsid gene segment which was incorporated into the genome. Immuneprecipitation of cell lysates with sera from rabbit immunized with inactivateded Sabin type 1 particles demonstrated the constitutive expression of the poliovirus P1 capsid protein from R2-18.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.193-196
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• 2001

IL-18. formerly known as IGIF(interferon -gamma inducing factor), is structurally IL-l related but functionally IL-12 related pro-inflammatory cytokine. The human IL -18(hIL-lS), like IL-$1{\beta}$, is synthesized as a biologically inactive precursor of 24kDa lacking a signal peptide, and then cleaved into an active mature form by cystein protease IL-$1{\beta}$ converting enzyme (ICE: caspase- 1), We tested if the mature hIL -18 can be expressed and secreted into culture medium by transforming the forming gene construct consisting of a mature hIL-18 gene fused to signal peptide of rice amylase lA. Secondly, we were tested if the pro- IL-18 could be processed into a biologically active form by caspase-l like protease in plant. Cell suspension culture was established from the leaf-derived calli of transgenic tobacco plant. Southern and Northern blot analysis indicated the expression of both pro-hIL-18 and mature hIL-18 plant cells. Western blot analysis introduced the protein products of pro- hIL -18 and mhIL -18 were observed in transigenic cell lines. In addition, the molecular size of recombinant pro-hILl-18 and mhIL-18 were estimated to be 24kDa and 18kDa, respectively. ELISA revealed that the amount of pro- hIL -18 was 1.3ug per gram of fresh weight calli. Moreover, the presence of mhIL-18 was detected in the culture medium and it appeared to be 25ug/L.

• 한국패류학회지
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• 제28권1호
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• pp.55-64
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• 2012

Ultrastructural studies of germ cell development during spermatogenesis and taxonomic values in mature sperm morphology of Argopecten irradians irradians were investigated by transmission electron microscopic observations. In the early stage of spermatid during spermiogenesis, a few granules and proacrosomal granules are formed by the Golgi complex. In the late stage of spermatid during spermiogenesis, a proacrosomal vesicle becomes an acrosomal vesicle in the acrosome through spermiogenesis. The sperm is approximately $ 45-48{\mu}m$ in length including a jar-shaped sperm nucleus (about $1.45{\mu}m$ long), an acrosome (about $0.34{\mu}m$ long) and tail flagellum. The axoneme of the sperm tail shows a 9+2 structure. As one of common characteristics of mature sperm morphologies in Pectinidae species in subclass Pteriomorphia, mature spermatozoon consists of the cone-shaped acrosomal vesicle and subacrosomal material on the invaginated jar-shaped nucleus. The acrosomal vesicle of this species is composed of electron high dense opaque part (material) from the base to the tip, as have seen in the species in the subclass Pteriomorphia. Exceptionally, five mitochondria are found in the sperm midpiece of this species, unlike four in most species of Pectinidae in subclass Pteriomorphia. However, the acrosomal vesicle of spermatozoa of A. irradians irradians resemble to those of other investigated Pectinidae species in subclass Pteriomorphia. Therefore, we can use sperm morphology as a tool in the resolution of taxonomic relationships within the Pectinidae species. These morphological charateristics of acrosomal vesicle belong to the family Pectinidae in the subclass Pteriomorphia.

• Molecules and Cells
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• 제28권6호
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• pp.553-558
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• 2009

In the periphery, a galectin-1 receptor, CD7, plays crucial roles in galectin-1-mediated apoptosis of activated T-cells as well as progression of T-lymphoma. Previously, we demonstrated that $NF-{\kappa}B$ downregulated CD7 gene expression through the p38 MAPK pathway in developing immature thymocytes. However, its regulatory pathway is not well understood in functional mature T-cells. Here, we show that CD7 expression was downregulated by Twist2 in Jurkat cells, a human acute T-cell lymphoma cell line, and in EL4 cells, a mature murine T-cell lymphoma cell line. Furthermore, ectopic expression of Twist2 in Jurkat cells reduced galectin-1-induced apoptosis. While full-length Twist2 decreased CD7 promoter activity, a C-terminal deletion form of Twist2 reversed its inhibition, suggesting an important role of the C-terminus in CD7 regulation. In addition, CD7 expression was enhanced by histone deacetylase inhibitors such as trichostatin A and sodium butyrate, which indicates that Twist2 might be one of candidate factors involved in histone deacetylation. Based on these results, we conclude that upregulation of Twist2 increases the resistance to galectin-1-mediated-apoptosis, which may have significant implications for the progression of some T-cells into tumors such as Sezary cells.