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Effects of Soy-isoflavonoid on Molecular Markers Related to Apoptosis in Mature and Ovariectomized Female Rats, and Mammalian Tumor Cell Lines  

Shin, Jang-In (Department of Food and Nutrition, Hannam University)
Lee, Mee-Sook (Department of Food and Nutrition, Hannam University)
Park, Ock-Jin (Department of Food and Nutrition, Hannam University)
Publication Information
Food Science and Biotechnology / v.14, no.6, 2005 , pp. 709-714 More about this Journal
Abstract
Alteration of molecular markers related to apoptosis of in vivo normal system and in vitro cancerous system by soy-isoflavonoid with estrogen was investigated. Down-regulation of Bcl-2 was accompanied by decreased expression of COX-2 (cyclooxygenase-2) in mature female rats treated with soy-isoflavonoid and estrogen. In ovariectomized rat system, Bax was regulated by higher concentration of soy treatment. Bax up-regulation by soy-isoflavonoid genistein treatment was observed in MCF-7 mammary cancer cell system. Estrogen without soy induced similar pattern of Bax expression as soy-isoflavonoid in vivo, but exhibited opposite trend in vitro. These findings suggest soy-isoflavonoid may have potential to induce apoptosis at higher concentrations through up-regulation of Bax or down-regulation of Bcl-2 expressions depending on normal or cancerous state, and physiological status of rats.
Keywords
soy-isoflavonoids; molecular markers related to apoptosis; mature estrogen-supplemented rats; ovariectomized rats; MCF-7 cells;
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1 The two phyto-oestrogens genistein and quercetin exert different effects on oestrogen receptor function /
[ Miodini, P.;Fioravanti, L.;Di Fronzo, G.;Cappelletti, V. ] / Br. J. Cancer
2 A review of the clinical effects of phytoestrogens /
[ Knight, D.C.;Eden, J.A. ] / Obstet. Gynecol
3 Cyclooxygenase-2 modulates cellular growth and promotes tumorigenesis /
[ Trifan, O.C.;Hla, T. ] / J. Cell. Mol. Med.
4 Estrogenic activity of flavonoids in mice. The importance of estrogen receptor distribution, metabolism and bioavailability /
[ Breinholt, V.;Hossaini, A.;Svendsen, G.;Brouwer, C.;Nielsen, S.E. ] / Food Chem. Toxicol.
5 The prognostic significance of altered cyclin-dependent kinase inhibitors in human cancer /
[ Tshilias, J.;Kapusta, L.;Slingerland, J. ] / Ann. Rev. Med.
6 NS398, a selective cyclooxygensae-2 inhibitor, induces apoptosis and downregulates bcl-2 expression in LNCaP cells /
[ Liu, X.H.;Yao, S.;Kirschenbaum, A.;Levine, A.C. ] / Cancer Res.
7 Urinary excretion of lignins and isotlavonoid phytoestrogens in Japanese men and women consuming a traditional diet /
[ Adlercreutz, H.;Honjo, H.;Higashi, A.;Fotsis, T.;Hamalainen, E.;Hasegawa, T.;Okada, H. ] / Am. J. Clin. Nutr.
8 Dietary effects on breast cancer risk in Singapore /
[ Lee, H.P.;Gourley, L.;Duffy. S.W.;Esteve, J.;Lee, J.;Day, N.E. ] / Lancet
9 Migration patterns and breast cancer risk in Asian-American women /
[ Ziegler, R.G.;Hoover, R.N.;Pike, M.C. ] / J. Nat. Cancer Inst.
10 Case-control study of phyto-oestrogens and breast cancer /
[ Ingram, D.;Sanders, K.;Kolybaba, M.;Lopez, D. ] / Lancet
11 Phytoestrogens: the biochemistry, physiology and implications for human health of soy soy-isotlavones /
[ Setchell, K.D.R. ] / Am. J. Clin. Nutr.
12 Soy soy-isotlavones-benefits and risks from nature's selective estrogen receptor modulators (SERMs) /
[ Setchell, K.D.R. ] / J. Am. Coll Nutr.
13 High dietary phytoestrogen intake is associated with higher bone mineral density in postmeno？pausal but not premenopausal women /
[ Mei, J.;Yeung, S.S.;Kung, A.W. ] / J. Clin. Endocrinol
14 Therapeutic approaches to bone diseases /
[ Rodan, G.A.;Martin, T.J. ] / Science
15 Nutritional and socioeconomic factors in relation to prostate cancer mortality: a cross-national study /
[ Herbert, J.;Hurley, T.;Olendzki, B.;Teas, J.;Yunsheng, M. ] / J. Natl. Cancer Inst.
16 The estrogenic activity and metabolism of certain soy-isotlavones in sheep /
[ Braden, A.;Hart, N.;Lamberton, J. ] / Aust. J. Agric. Res.
17 Bcl-2 is not reduced in the death of MCF7 cells at low genistein concentration /
[ Leung, L.K.;Wang, T.T. ] / J. Nutr.
18 Different effects of genistein on molecular markers related to apoptosis in two phenotypically dissimilar breast cancer cell lines /
[ Xu, J.;Loo, G. ] / J. Cell Biochem.
19 Effects of onion extracts on both normal and neoplastic cell proliferation and the inhibition of tumor growth in mouse /
[ Han, D.;Han, J.;Oh, S.;Rho, S.N. ] / Food Sci. Biotechnol.
20 The role of prostaglandin synthesis in prostate cancer /
[ Badawi, A.F. ] / Brrt. J. Urol. Int.
21 Phyto-estrogens and western diseases /
[ Adlercreutz, H.;Mazur, W. ] / Ann. Med.
22 Steroid and phytoestrogen binding to sheep uterine receptors in vitro /
[ Shutt, D.A.;Cox, I. ] / J. Endocrinol
23 Phytoestrogen interaction with estrogen receptors in human breast cancer cells /
[ Martin, P.M.;Horwitz, K.B.;Ryan, D.S.;McGuire, W.L. ] / Endocrinol
24 Molecular effects of genistein on estrogen receptor mediated pathways /
[ Wang, T.T.Y.;Sathyamoorthy, N.;Phang, J.M. ] / Carcinogenesis
25 Soy diets containing varying amounts of genistein stimulate growth of estrogen-dependent (MCF-7) tumors in a dose-dependent manner /
[ Allred, C.D.;Allred, K.F.;Ju, Y.H.;Virant, S.M.;Helferich, W.G. ] / Cancer Res.
26 Differential response of estrogen receptor and estrogen receptor <TEX>${\beta}$</TEX> to partial estrogen agonists/antagonists /
[ Barkhem, T.;Carlsson, B.;Nilsson, Y.;Enmark, E.;Fustafsson, J.;Nilsson, S. ] / Pharm.
27 Potentail health benefits of dietary phytoestrogens: A review of the clinical, epidemiological, and mechanistic evidence /
[ Thom, D.M.;Gardner, C.D.;Haskell, W.L. ] / J. Clin. Endocrinol. Metab.
28 Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor /
[ Kuiper, G.G.J.M.;Lemmen, J.G.;Carlsson, B.;Corton, J.C.;Safe, S.H.;van der Saag, P.T.;van der Burg, B.;Gustafsson. J. ] / Endocrinol.
29 Differential effects of xenoestrogens on coactivator recruitment by estrogen receptor(ER) <TEX>${\alpha}$/TEX> and ER <TEX>${\beta}$<TEX> /
[ Routledge, E.J.;White, R.;Parker, M.G.;Sumpter, J.P. ] / J. Biol. Chem.
30 Geinstein inhibits proliferation similarly in estrogen receptor-positive and negative human breast carcinoma cell lines characterized by p21WAFIICIPI induction, G2/M arrest, and apoptosis /
[ Shao, A.;Alpaugh, M.L.;Fontana, J.A.;Barsky, S.H. ] / J. Cell Biochem.
31 Estrogen regulation of cell cycle progression in breast cancer cells /
[ Prall, O.W.;Rogan, E.M.;Sutherland, R.L. ] / J. Steroid Biochem. Mol. Biol.
32 Genistein inactivates bcl-2, delays the G2/M phase of the cell cycle, and induces apoptosis of human breast adenocarcinoma MCF-7 cells /
[ Constatinou, A.I.;Kamath, N.;Murley, J.S. ] / Eur. J. Cancer
33 The proto-oncogene BcI-2 and its role in regulation apoptosis /
[ Kroemer, G. ] / Nature Med.
34 Induction of apoptosis in breast cancer cells MDA-MB-231 by genistein /
[ Li, Y.;Upadhyay, S.;Bhuiyan, M.;Sarkar, F.H. ] / Oncogene
35 Genistein-induced upregulation of p21WAFl, downregulation of cyclin B, and induction of apoptosis in prostate cancer cells /
[ Davis, I.N.;Singh, B.;Bhuiyan, M.;Sarkar, F.H. ] / Nutr. Cancer
36 Apoptosis in the pathogenesis and treatment of disease /
[ Thompson, C.B. ] / Science
37 Bcl-2: Prevention of apoptosis as a mechanism of drug resistance. Hematol Oncol /
[ Reed, J.C. ] / Clin. North Am.
38 Overexpression of Bax sensitizes human pancreatic cancer cells to apoptosis induced by chemotherapeutic agents /
[ Xu, Z.W.;Friess, H.;Buchler, M.W.;Solioz, M. ] / Cancer Chemother. Pharmacol
39 bcl-2/bax expression and p53 gene status in human bladder cancer. Relationship to early recurrence with intravesical chemotherapy after resection /
[ Ye, D.;Li, H.;Qian, S.;Sun, Y.;Zheng, J.;Ma, Y. ] / J. Urol.
40 Induction of apoptosis in breast cancer cells MDA-MB-231 by genistein /
[ Li, Y.;Upadhyay, S.;Bhuiyan, M.;Sarkar, F.H. ] / Oncogene
41 Effects of estrogen on apoptotic pathways in human breast cancer cell line MCF-7 /
[ Wang, T.T.Y.;Phang, J.M. ] / Cancer Res.
42 Many actions of Cyclooxygenase-2 in cellular dynamics and in cancer /
[ Cao, Y.;Prescott, S.M. ] / J. Cell Physiol
43 The role of cyclooxygenases in inflammation, cancer and development /
[ Williams, C.S.;Mann, M.;DuBois, R.N. ] / Oncogene
44 Urinary excretion of isotlavonoids and the risk of breast cancer /
[ Zheng, W.;Dai, Q.;Custer, L.;Shu, X.O.;Wen, W.Q.;Jin, F.;Franke, A.A. ] / Cancer Epidemiol. Biomark. Prev.
45 Soy diets containing varying amount of genistein stimulated growth of estrogen dependent (MCF -7) tumors in a dose-dependent manner /
[ Allred, C.D.;Allred, K.F.;Ju, Y.H.;Virant, S.M.;Helferich, W.G. ] / Cancer Res.
46 Estrogenic and antiproliferative properties of genistein and other tlavonoids in human breast cancer cells in vitro /
[ Zava, D.T.;Duwe, G. ] / Nutr. Cancer
47 Phyto-estrogens and western diseases /
[ Adlercreutz, H.;Mazur, W. ] / Ann. Med.