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http://dx.doi.org/10.1007/s10059-009-0150-8

Twist2 Regulates CD7 Expression and Galectin-1-Induced Apoptosis in Mature T-Cells  

Koh, Han Seok (Department of Life Science and Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University)
Lee, Changjin (Department of Biological Sciences, Research Center for Functional Cellulomics, Seoul National University)
Lee, Kwang Soo (Department of Life Science and Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University)
Park, Eun Jung (Immune and Cell Therapy Branch, National Cancer Center)
Seong, Rho H. (Department of Biological Sciences, Research Center for Functional Cellulomics, Seoul National University)
Hong, Seokmann (Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University)
Jeon, Sung Ho (Department of Life Science and Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University)
Publication Information
Molecules and Cells / v.28, no.6, 2009 , pp. 553-558 More about this Journal
Abstract
In the periphery, a galectin-1 receptor, CD7, plays crucial roles in galectin-1-mediated apoptosis of activated T-cells as well as progression of T-lymphoma. Previously, we demonstrated that $NF-{\kappa}B$ downregulated CD7 gene expression through the p38 MAPK pathway in developing immature thymocytes. However, its regulatory pathway is not well understood in functional mature T-cells. Here, we show that CD7 expression was downregulated by Twist2 in Jurkat cells, a human acute T-cell lymphoma cell line, and in EL4 cells, a mature murine T-cell lymphoma cell line. Furthermore, ectopic expression of Twist2 in Jurkat cells reduced galectin-1-induced apoptosis. While full-length Twist2 decreased CD7 promoter activity, a C-terminal deletion form of Twist2 reversed its inhibition, suggesting an important role of the C-terminus in CD7 regulation. In addition, CD7 expression was enhanced by histone deacetylase inhibitors such as trichostatin A and sodium butyrate, which indicates that Twist2 might be one of candidate factors involved in histone deacetylation. Based on these results, we conclude that upregulation of Twist2 increases the resistance to galectin-1-mediated-apoptosis, which may have significant implications for the progression of some T-cells into tumors such as Sezary cells.
Keywords
CD7; galectin-1-mediated apoptosis; histone deacetylation; Twist2;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
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