• Fisheries and Aquatic Sciences
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• 제25권7호
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• pp.390-402
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• 2022

In this study we investigate physical and chemical characteristics of immature and mature skipjack tuna (Katsuwonus pelamis) roes in fresh and dried forms. Fresh roes were studied for histological structure and also dried by three methods: hot air drying (HD), vacuum drying (VD) and freeze drying (FD). The obtained roe powders were analysed for proximate composition, color value, fatty acid composition, amino acid profile, equivalent umami concentration (EUC) and protein pattern. Unyolked oocytes were more common in immature roes, while fully yolked oocytes were more common in mature roes. All dried tuna roes contained high content of protein and lipid (69.31%-70.55% and 11.14%-16.02%, respectively). The powders obtained by FD provided the highest lightness value (L^*). The main fatty acid found in all roe powders was docosahexaenoic acid (DHA) (23.49%-27.02%). Glutamic acid, leucine, and aspartic acid were the three most abundant amino acids found in the powders (13.58-14.61, 8.06-8.42, and 7.81-8.39 g/100 g of protein, respectively). The mature roe powder obtained from HD provided the highest EUC value (73.09 g monosodium glutamate/100 g of samples). The protein band at molecular weight of 97 kDa (vitelline) represented the major protein. Therefore, dried tuna roe could be a functional ingredient source of protein and lipid rich in DHA and it also has potential to be used as taste enhancer with umami compound.

• 대한핵의학회지
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• 제39권1호
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• pp.26-33
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• 2005

목적 : 이 연구는 $^{99m}Tc$-HMPAO에 표지된 미성숙 또는 성숙 수지상 세포의 마우스 생체 내 분포와 이동 양상에 대해 알아보고자 하였다. 대상 및 방법: 마우스의 대퇴골과 경골의 골수로부터 수지상 세포를 배양하고 미성숙, 성숙 수지상세포를 $^{99m}Tc$-HMPAO로 표지하였다. 방사성 표지 전후에 수지상 세포의 기능 및 표현형의 변화 유무를 알기 위해 동종 혼합 림프구 반응 (allogeneic mixed lymphocyte reaction)과 형광 활성 세포 선별 (fluorescence-activated cell sorting)을 시행하였다. 정맥 주사된 수지상 세포의 생체 내 이동은 감마 카메라 영상과 생체 분포 실험을 통하여 평가하였고, 피하 종양 마우스 모델과 대조군에서 비교하였다. 폐, 간, 비장, 신장, 종양 등 조직에서 그램 당 주사량의 백분율(%ID/g)을 계산하였다. 결과: 미성숙, 성숙 수지상 세포의 표지 효율은 각각 $60.4{\pm}5.4%$와 $61.8{\pm}6.7%$ 였다. 수지상 세포의 정맥주사 후 방사능은 폐에서 가장 먼저 관찰되었고, 이후 간과 비장에 분포되었다. 성숙 수지상 세포가 미성숙 수지상 세포에 비해 비장으로 더 많이 이동하였다(대조군; $38.3{\pm}4.0%\;vs.\;32.2{\pm}4.1%$, 종양이식 군: $40.4{\pm}4.1%\;vs.\;35.9{\pm}3.8%$, p<0.05). 종양으로의 이동 역시 성숙 수지상 세포가 미성숙 수지상 세포에 비해 더 많은 비율을 보였다($2.4{\pm}0.3%\;vs\;1.7{\pm}0.2%$; p=0.034). 결론: $^{99m}Tc$-HMPAO 에 표지된 수지상 세포를 이용하여 마우스 생체 내 이동을 실시간 영상화 할 수 있었다. 마우스 정맥에 주사되었을 때, 더 많은 비율의 성숙 수지상 세포가 미성숙 수지상 세포에 비해서 비장이나 종양으로 이동함을 알 수 있었다.

• 한국연초학회지
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• 제18권1호
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• pp.5-11
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• 1996

Field experiment was conducted to get the basic information about the difference in color and plastid pigments at the different stage of harvest of flue-cured tobacco cultivated in paddy field, and compared to upland ones. All tested crops were primed in lots of 3 to 4 at each priming, beginning at the bottom of the plant. Leaf pigments and chromatic values of tobacco leaves were determined in samples collected at weekly intervals, and according to analyses obtained from 4 stalk positions. By the degree of maturity, leaves harvested were separated with visual characters into four classes of immature, mature, ripe and mellow. regardless of stalk position, chlorophyll contents was higher in the order of immature > mature > ripe > mellow, respectively. In fresh leaves, chlorophyll levels was higher with ascending stalk position. chlorophyll a and b in cured leaves were less than 10% of the amount present at harvest, and it was estimated that maturity condition also influenced the rates of degradation of plastid pigments. Fully mature leaves of upland diverted from paddy field showed slightly higher in chlorophyll contents than in those of upland tobacco ones but somewhat lowered in $L^*$ and $b^*$ values, and there was no difference in $a^*$ value.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2003년도 추계학술발표대회 논문요약집
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• pp.52-52
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• 2003

For the evaluation of hormonal control of spermiation in fish, a method to quanify the spermiation response or mature Rhynchocypris oxycephalus (Sauvage and Dabry) to hormonal therapy is described. Spermatocrit was determined after 7 min centrifugation at 18,000 $\times$ｇ and sperm density was estimated by a standard hemocytormer method. Sperm density can be predicted from spermatocrit since their relationship is linear as described by the regression equation, Y=3.68X-27,18 ($R^2$=0.82, N=50), where Y is spermatocrit and X is sperm density. Milt production by mature R. oxycephalus was highest at 24 h after injection of 1,000 IU human chorionic gonadotropin (HCG) and 50 $\mu\textrm{g}$ luteinizing hormone-releasing hormone analogue (LHRHa) per kg body weight. Increased milt production coincided with low spermotocrit and sperm density levels. These results demonstrate that spermition in mature R. oxycephalus can be reliably evaluated by a spermatocrit method and that HCG and LHRHa are effecove in stimulating of spermiation in this species.

• 한국수정란이식학회지
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• 제30권2호
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• pp.83-89
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• 2015

The sexual maturation occurred by the changes of steroid hormones was known to sex-dependently and/or age-dependently regulate the lipid metabolism in various animal species. Our current study demonstrates that lipid and its functional fatty acids can be changed depending on the status of sexual maturation. Of the functional fatty acids, ${\gamma}$-linolenic acid (GLA; 18:3n-6) is an important factor for maintaining human health. The purpose of our study was to investigate the level of GLA in mice with different stages of sexual maturation. To this end, the longissimus muscle (LM) of immature (3-week-old) and mature (7-week-old) female mice was analysed for the fatty acid composition by gas chromatography. Furthermore, both gene and protein level of ${\Delta}6$ desaturase (FADS2) which is involved in GLA metabolism by real time PCR and Western blotting, respectively. Mature females showed greater (P<0.05) serum $17{\beta}$-estradiol (E2) level and LM GLA contents than immature group. The mRNA and protein levels of FADS2, which converts precursor linoleic acid into GLA, were higher (P<0.05) in mature female mice than in immature mice. In conclusion, these results show that sexual maturation of female mice induces GLA and FADS2 contents in LM.

• 대한치과교정학회지
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• 제13권2호
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• pp.155-163
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• 1983

Many different appliances have been used to move teeth or to attempt to inhibit or stimulate growth of the jaws. Especially, Orthopedic force was used to apply a constant force on the mandible. Various orthopedic forces bring the changes of surrounding tissue, growth and development on the mandibular joint. Author carried out this study to find the histological changes of the mandibular joint in rats when the mandible applied posterior displacement. For the purpose of this study, $009\times036inch$ closed coil spring and 030inch orthopedic wire were used between the post occipital region and the chin region in order to render the orthopedic forces, posteriorly. The experimental animals vu. used 1 month old (GroupI), 3 months old (Group II), and 12 months old (Group III) rats. Following results were obtained; 1. At the beginning of the experimental Group I, there were predominant increment of Cartilage layer in the posterior parts of condylar head. Especially, the mature chondrocyte zone was increased. 2. In the experimental Group II, the chondroblastic zone was decreased while it showed slightly increased mature cartilage zone. 3. In the experimental Group III, the mature chondrocyte zone was disappeared gradually and there was a tendency to reduce the endochondral bone formation in condylar head. 4. After 30 days of experimental Group I and in experimental Group II, there was a tendency to decrease the immature cellular zone in the glenoid fossa by pressure. 5. There were no histological changes in the articular disc by pressure.

• International Journal of Costume and Fashion
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• 제12권1호
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• pp.49-63
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• 2012

The objectives of this study were to investigate appearance management behaviors and hairstyle preferences in accordance with personality types and self-image, and to examine the characteristics that influence simultaneously the appearance management behaviors. A survey was used for as for this research. The personality properties of introversion and extroversion were further divided, based on the aspects of emotions, thoughts, behaviors, and interpersonal psychological functions. The subjects were 383 girls in their first year of high school in Seoul. The extrovert type showed a higher level of management for clothing, hair and face than the introvert type. The emotional extrovert type and the interpersonal extrovert type showed the highest level of clothing management, the active extrovert type showed the highest level of face management, and the interpersonal introvert type showed a very low level of hail management. The high school girls who had a higher social and mature self-image showed a higher level of clothing, hair and face management. Introverted girls preferred a mid-length straight hairstyle more than extroverted girls. The higher the social and mature self-images girls had, the more they preferred a long wave hairstyle. The higher the cute image they had, the more they preferred wave hairstyle. The entire appearance management behavior was affected significantly by extroversion, mature image, and allowance, simultaneously.

• 한국작물학회지
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• 제44권4호
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• pp.309-315
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• 1999

Rice seedling test was conducted to check the loging tolerance at ripening stage through evaluating the root characters. Thirteen Korean and foreign rice cultivars with direct seeding adaptable or high quality characteristics were grown in a cell pot and under submerged paddy. The root characters and pushing resistance of rice hill were determined at seedling and ripening stage, respectively. The diameter of crown root at the 7th and 8th leaf stages was thicker in lodging tolerance cultivars than those of others and showed significant-positive correlation with both pushing resistance and crown root diameter of mature plants. Also, the tensile strength of crown root at the 7th and 8th leaf stage showed highly positive correlation with the tensile strength of crown root of mature plants. The number of crown root at 7th leaf stage was significant-positively correlated with that of mature plant. The diameter of seminal root was not significantly correlated with the diameter of crown root throughout the whole growth stage. These results indicate that the diameter, tensile strength and number of crown root associated with root lodging tolerance can be detected with the seedling at about 7th or 8th leaf stage, and the seedling test using the cell pot is an useful and practical method to select lodging tolerant cultivars or lines of rice based on root characters, especially diameter of crown root.

• 한국식품영양과학회지
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• 제14권1호
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• pp.47-50
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• 1985

한국인 중류층 수유부들로부터 분만 후 70일까지 채취한 유즙의 인 지방질의 자방산 조성이 수유기간의 경과에 어떻게 변화하는지 살펴보았다. 8 : 0 은 이행유 단계에서, 그리고 10 : 0, 12 : 0, 14 : 0 등은 성숙유 I단계에서 각각 유의하게 증가하였다. w6계 대사산물들(20 :2w6, 20 : 4w6, 22 : 4w6)은 수유기간의 경과에 따라 점진적인 감소의 경향을 나타내었다. 24 : 1w9는 이행유 단계에서 유의한 증가의 수준을 유지하다가 그 이후는 초유 수준 이하로 유의하게 감소하였다.

• Journal of Biosystems Engineering
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• 제24권4호
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• pp.365-372
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• 1999

Physical characteristics of stem (ile., length and diameter) and branch, and detachment force of mature and immature fruits of 12 species of the Lycium chinese Mill at a cultivar were determined. The number of stems and branches were counted after the first and second pruning of each plant. Length and diameter of the stem were average of 113.5 cm and 9.5 mm for 12 species of the Lycium chinese Mill, respectively. Average number of stem and number of branch after the first and second pruning in each plant were 5, 30, and 61, respectively. Diameter of major and minor axis of the fruit was average of 13.8mm and 8.3mm, respectively, and the sphericity of fruits was average of 0.7 for 12 species of Lycium chinese Mill. Detachment force of mature and immature fruits was average of 1 N and 2.7 N, respectively, for 12 species of the Lycium chinese Mill. The maximum and minimum detachment force of the mature fruits was 2.06 N and 0.39 N, respectively, and that of the immature fruits was 3.72 N and 1.27 N, respectively. The force-weight ratio showed a decreasing trend as the weight of fruit increasing for all samples.