• 제목/요약/키워드: material control

검색결과 6,054건 처리시간 0.034초

Large Increase in Leuconostoc citreum KM20 Dextransucrase Activity Achieved by Changing the Strain/Inducer Combination in an E. coli Expression System

  • Ko, Jin-A;Jeong, Hyung-Jae;Ryu, Young-Bae;Park, Su-Jin;Wee, Young-Jung;Kim, Do-Man;Kim, Young-Min;Lee, Woo-Song
    • Journal of Microbiology and Biotechnology
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    • 제22권4호
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    • pp.510-515
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    • 2012
  • A recombinant putative dextransucrase (DexT) was produced from Leuconostoc citreum KM20 as a 160 kDa protein, but its productivity was very low (264 U/l). For optimization, we examined enzyme activity in 7 Escherichia coli strains with inducer molecules such as lactose or IPTG. E. coli BL21-CodonPlus(DE3)-RIL exhibited the highest enzyme activity with lactose. Finally, DexT activity was remarkably increased by 12-fold under the optimized culture conditions of a cell density to start induction ($OD_{600}$) of 0.95, a lactose concentration of 7.5 mM, and an induction temperature of $17^{\circ}C$. These results may effectively apply to the heterologous expression of other large DexT genes.

저온저장고의 뉴로-퍼지 제어시스템 개발 (Development of Neuro-Fuzzy System for Cold Storage Facility)

  • 양길모;고학균;홍지향
    • Journal of Biosystems Engineering
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    • 제28권2호
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    • pp.117-126
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    • 2003
  • This study was conducted to develop precision control system fur cold storage facility that could offer safe storage environment for green grocery. For that reason of neuro-fuzzy control system with learning ability algorithm and single chip neuro-fuzzy micro controller was developed for cold storage facility. Dynamic characteristics and hunting of neuro-fuzzy control system were far superior to on-off and fuzzy control system. Dynamic characteristics of temperature were faster than on-off control system by 1,555 seconds(123% faster) and fuzzy control system by 460 seconds(36.4% faster). When system was arrived at steady state. hunting was ${\pm}$0.5$^{\circ}C$ in on-off control system, ${\pm}$0.4$^{\circ}C$ in fuzzy control system, and ${\pm}$0.3$^{\circ}C$ in neuro-fuzzy control system. Hunting of humidity and wind velocity was also controlled precisely by 70 to 72.5% and 1m/s For storage experiment with onion, characteristics of neuro-fuzzy control system were tested. Dynamic characteristics of neuro-fuzzy control system made cold storage facility conducted precooling ability and minimized hunting.

The history of IFMFC - The accumulated knowledge and experience of the magnetic force control with IFMFC

  • Watanabe, Tsuneo
    • 한국초전도ㆍ저온공학회논문지
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    • 제18권1호
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    • pp.6-9
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    • 2016
  • The history of IFMFC (International Forum on Magnetic Force Control) shows the usefulness of the magnetic force control in the fields of the environment and material resource in Japan, Korea and China. The IFMFC started in 2010 and has been organized in every year. This paper shows the application of the magnetic force control in each countries with the accumulated knowledge and experience of the magnetic force control with IFMFC.

자기 변형 작동기를 이용한 진동 절연 시스템 (The vibration isolating system using a magnetostrictive actuator)

  • 정학근;박기환
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 1997년도 한국자동제어학술회의논문집; 한국전력공사 서울연수원; 17-18 Oct. 1997
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    • pp.276-279
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    • 1997
  • When a magnetostrictive material is exposed to a magnetic field, its geometry changes due to a magnetostrictive effect. The magnetostriction is analogous to the piezoeletricity. The displacement of the magnetostrictive material is proportional to the applied current while that of the piezoelectric material is proportional to the voltage. A magnetostrictive material generates large displacement and higher compressive force compared with a piezoeletric material. These advantages provide a good performance of a vibration isolation of a platform. In this work, it is applied to a driving actuator for vibration isolation of a platform. The properties of a magnetostrictive material are investigated in terms of hysteresis and displacement vs. applied current for a various preload. Modeling of the displacement of the vibration isolating actuator is performed as it behaves as a flow source. A sliding mode controller is designed to demonstrate the ability of the magnetostrictive actuator to reduce the vibration at the platform. The effectiveness of the proposed scheme is demonstrated through experimental works. The experimental results of the vibration of the platform axe presented in terms of time response and frequency response.

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객체지향 설계 및 시뮬레이션을 이용한 자동 물류 핸들링 시스템의 제어 로직 검증 (Validation of the Control Logic for Automated Material Handling System Using an Object-Oriented Design and Simulation Method)

  • 한관희
    • 제어로봇시스템학회논문지
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    • 제12권8호
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    • pp.834-841
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    • 2006
  • Recently, many enterprises are installing AMSs(Automated Manufacturing Systems) for their competitive advantages. As the level of automation increases, proper design and validation of control logic is a imperative task for the successful operation of AMSs. However, current discrete event simulation methods mainly focus on the performance evaluation. As a result, they lack the modeling capabilities for the detail logic of automated manufacturing system controller. Proposed in this paper is a method of validation of the controller logic for automated material handling system using an object-oriented design and simulation. Using this method, FA engineers can validate the controller logic easily in earlier stage of system design, so they can reduce the time for correcting the logic errors and enhance the productivity of control program development Generated simulation model can also be used as a communication tool among FA engineers who have different experiences and disciplines.

압전 변압기의 제어 방식에 따른 모델링 및 안정화분석 (Stabilization Analysis of Piezo-electric Converter for PFM and PWM Control)

  • 윤석택;박성우;원영진;이진호;김진희
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2009년도 하계학술대회 논문집
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    • pp.401-401
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    • 2009
  • Recently, demands for the development of compact, lightweight power supplies with higher power density and higher efficiency have been increased. Since Piezoelectric Transformer (PT) was emerged in device and material industry, it has been suggested as a viable alternative to the magnetic transformer in some applications. PT has some advantages such as low profile and mechanical energy transfer with little electromagnetic interface (EMI). Also, PT can provide high voltage stepping ratio with good isolation and requires no copper windings saving copper usage especially for large voltage conversion differences. Conventional control of PT converter has mainly two-way. One is the pulse frequency modulation (PFM) control method and the other is the pulse width modulation (PWM) control with frequency fixed method. It is known that the maximum PT efficiency can be obtained when it operates near the resonant frequency of the PT. And, also PT's resonant frequency moves according to the load condition. Therefore, selection of PT converter control method is very difficult. This paper analyzes general piezo-electric converter modeling and proposes a guide-line to selection of control method and stabilization control.

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소형렌즈 성형시스템 개발 및 힘제어에 관한 연구 (Development of Molding System for Manufacturing a Small Lens and Its Force Control)

  • 국금환;정동연;김갑순
    • 한국정밀공학회지
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    • 제25권2호
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    • pp.57-64
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    • 2008
  • This paper describes the development of a small lens molding system for manufacturing the small lens like lens of a cellular phone, a small digital camera and so on. In order to manufacture a small lens, firstly, the raw material for lens with spherical shape should be manufactured by processing a glass material, secondly, the mold inserted the raw material for lens should be heated till its molding temperature in the electric furnace, finally, the small lens is manufactured by applying the force using pressuring control system. In this paper, the small lens molding system with the function of force control and velocity control was developed. It is composed of an electric furnace and its temperature control system, a pressuring control system, a body, and so on. The temperature characteristic test of the electric furnace, the force and velocity characteristic test of the pressuring control system were carried out. It was confirmed that the developed system had good functions for manufacturing a small lens.

전사방식 마이크로 광 조형에서 복합 재료의 미세구조물 제작을 위한 수지 교한 시스템 개발 (Development of Material Switching System for Microstructure with Multiple Material in Projection Microstereolithography)

  • 조광호;박인백;하영명;김민섭;이석희
    • 한국정밀공학회지
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    • 제28권8호
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    • pp.1000-1007
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    • 2011
  • For enlarging the applications of microstereolithography, the use of diverse materials is required. In this study, the material switching system (MSS) for projection microstereolithography apparatus is proposed. The MSS consists of three part; resin level control, resin dispensing control, and vat level control. Curing characteristic of materials used in fabrication has been identified. Through repeated fabrication of test models, the critical fabrication error is investigated and a possible solution to this error is suggested. The developed system can be applied to improve the strength of microstructure and extended to fabricate an array of microstructures with multiple materials.

습도조절용 세라믹패널 및 도료의 흡·방습성능 평가 (Performance Evaluation of Water Vapour Adsorption & Desorption Properties of Ceramic Panel and Painting Materials for Humidity Control)

  • 장건영;류동우
    • 대한건축학회논문집:구조계
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    • 제34권3호
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    • pp.43-52
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    • 2018
  • This study is conducted to evaluate the performance of ceramic panels and painting materials for humidity control which are developed in non-plastic room temperature hardening structure as part of a project to improve a residential environment for the low-income class, rather than the performance of high-priced humidity control materials that are produced with the existing plasticity processing. The testing methods included the measurements of absorption & desoprtion of humidity per material; Mock-up Testing; an evaluation method of comparing the absorption & desoprtion performances of Ecocarat, ceramic panels and painting materials through Living Lab. According to the measurements of absorption & desoprtion per material, ceramic panels, E panel, and ceramic painting material showed 73.3g/m2, 96.6g/m2, and 111.1g/m2, respectively. That is, the performance of humidity control of each material was found to be good in the order of: Ceramic Paint > E panel > Ceramic Panel. According to performance evaluation testing with Mock-up test and Living Lab, Ceramic Paint, Ecocarat, and Ceramic Panels showed better absorption & desoprtion performances in the order.

Westgard Multi-Rules의 효율적 적용과 조치사항의 개선 (Efficient Application of Westgard Multi-Rules and Quality Control Implementation Improvement)

  • 정흥수;오윤정;배진수;백진영;황보라;신용환
    • 핵의학기술
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    • 제21권1호
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    • pp.60-64
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    • 2017
  • 검사의 질 향상과 국제표준화의 상용화 정도관리물질을 이용한 Westgard multi-rules 적용의 유용성은 이미 알려져 있다. 그러나 핵의학 체외검사의 특성상 정도관리물질과 환자검체의 동시 계측으로 인한 측정횟수의 증가에 따라 Westgard multi-rules법을 적용함에 있어 어려움이 있다. 이에 본 연구는 핵의학 체외검사에서 상용화 정도관리물질을 이용한 Westgard multi-rules 적용의 유용성과 보완, 개선을 통해 내부정도관리의 효율성 향상을 조사하였다. 2013년 01월부터 2016년 06월까지 삼성서울병원 핵의학과 체외검사실 통합의료시스템에 기록된 총 282건의 적용된 계통오차 multi-rules (22s, 101s)과 117건의 조치사항 기록을 분석하였다. 조치사항은 multi-rules 중 계통오차의 규칙이 적용 되었을 때 기록하는 원인분석으로 정도관리물질 오류, 실험과정 오류, 검사키트 로트번호 관리 오류, 기타 등 총 4개의 대분류로 구성하였다. Westgard multi-rules 적용을 통해 조치사항을 분석한 결과 정도관리물질 오류가 62건, 실험과정 오류가 24건, 검사키트 로트번호 관리오류가 18건, 기타 13건으로 분류되었다. 정도관리물질 오류를 방지하고자 개선사항으로 기존에 각 검사자마다 사용하던 방식을 담당자 지정 방식으로 변경하여 모든 검사의 하루 소비량을 분주하여 공동사용을 하였고, 나머지 오류를 방지하고자 검사 전후 모든 과정을 표준화 하여 검사실내 어느 검사자가 시행 하더라도 일원화할 수 있게 하였다. 정도관리물질 오류를 개선한 결과 해동 후 2일 이내 신선한 물질을 사용 가능하였고 같은 물질을 사용하는 검사끼리 비교가 가능해져 물질에 의한 오류인지 명확해짐으로 계통오차 발생원인이 정도관리물질 오류로 기록하는 건수가 줄어들었다. 또한 정도관리물질의 로트번호 변경 시 교체시기가 같아 관리가 용이해졌고, 물질 사용량의 감소로 경제적 효과를 얻을 수 있었다. 그리고 검사표준화 적용 후, 계통오차의 규칙인 22s와 101s의 발생건수가 개선 전 보다 월 평균 2건 이상 줄어드는 결과를 보였다. Multi-rules의 적용을 통한 계통오차의 빠른 확인을 위해 정도관리물질의 체계적인 관리와 목표값과 표준편자의 설정 및 관리가 바탕이 되어야하며, 계통오차 발생 시 검사의 원인분석을 통한 조치사항을 기록하는 것이 중요함을 확인하였다. 본 실험의 결과로 Westgard multi-rules 적용 분석을 통해 발생 오류의 기재와 원인을 효율적으로 분석함으로써 핵의학 검사 내부정도관리의 질적 향상과 정확하고 신속한 결과보고에 기여할 것으로 사료된다.

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