• 제목/요약/키워드: matK

검색결과 1,055건 처리시간 0.039초

matK와 rbcL DNA 바코드 분석을 통한 반하(半夏) 및 반하(半夏) 유사 한약재 유전자 감별 (Molecular Authentication of Pinelliae Tuber from its adulterants by the analysis of DNA barcodes, matK and rbcL genes)

  • 이영미;문병철;지윤의;김욱진;김호경
    • 대한본초학회지
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    • 제28권6호
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    • pp.53-58
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    • 2013
  • Objectives : Pinelliae Tuber has been used as a typical unauthentic herbal medicines. Due to the morphological similarity between Pinelliae Tuber and adulterants, the correct authentication is very difficult. Therefore, we introduced DNA barcode to establish a powerful tool for the authentication of Pinelliae Tuner from adulterants. Methods : To obtain DNA barcode regions, genomic DNA was extracted from nineteen specimens of Pinellia ternata, Pinellia pedatisecta, Pinellia tripartita, and Typhonium flagelliforme, and matK and rbcL genes were amplified. For identification of species specific sequences and analysis phylogenetic relationship, a comparative analysis were performed by the ClastalW and UPGMA based on entire sequences of matK and rbcL genes, respectively. Results : In comparison of two DNA barcode sequences, we elucidated the phylogenetic relationship showing distinct four groups depending on species and identified 40 and 20 species specific nucleotides enough to distinguish each species from matK and rbcL gene, respectively. The sequence differences at the corresponding positions were avaliable genetic marker nulceotides to discriminate the correct species among analyzed four species. These results indicated that phylogentic and comparative analysis of matK and rbcL genes are useful genetic markers to authenticate Pinelliae Tubers. Conclusions : The marker nucleotides enough to distinguish P. ternata, P. tripatrita, P. peditisecta, and T. flagelliform, were observed at 40 positions in matK gene and 20 positions in rbcL gene sequence, respectively. These differences can be used to authenticate Pinelliae Tuber from adulterants as well as discriminate each four species.

Deposition and Characterization of Graphene Materials Deposited through Thermal Chemical Vapor Deposition

  • Kwon, Kyoung-Woo;Bae, Seung-Muk;Yeop, Moon-Soo;Kim, Ji-Soo;Ko, Myong-Hee;Jung, Min-Wook;An, Ki-Seok;Hwang, Jin-Ha
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2012년도 제43회 하계 정기 학술대회 초록집
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    • pp.362-362
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    • 2012
  • Graphene-based materials have been gaining the unprecedented academic and industrial applications, due to the unique charge transport as a new kind of 2-dimensional materials. The applications incorporate electronic devices, nonvolatile memories, batteries, chemical sensors, etc. based on the electrical, mechanical, structural, optical, and chemical features newly reported. The current work employs thermal chemical vapor deposition involving H2 and CH4, in order to synthesize the 2-dimensional graphene materials. The qualitative/quantitative characterizations of the synthesized graphene materials are evaluated using Raman spectroscopy and Hall Measurements, In particular, the effect of processing variables is systematically investigated on the formation of graphene materials through statistical design of experiments. The optimized graphene materials will be attempted towards the potential applications to flat-panel displays.

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Robust High Gain Adaptive Output Feedback Tracking Control for Nonlinear Systems

  • Kohara, Koshiro;Mizumoto, Ikuro;Iwai, Zenta;Michino, Ryuji;Kumon, Makoto
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 2000년도 제15차 학술회의논문집
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    • pp.444-444
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    • 2000
  • For a class of nonlinear systems which satisfy a certain condition so called output feedback exponential passivity (OFEP), it is well known that one can easily design a high-gain output feedback control system. The designed high-gain controller has simple structure and high robustness. However, from the viewpoint of practical application, it is important to consider a robust control scheme for controlled systems for which some of the assumptions of output feedback stabilization are not valid. In this paper. we deal with a design problem of the robust high-gain adaptive output feedback control for the above-mentioned class of nonlinear systems with uncertain nonlinearities and/or disturbances.

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실리콘웨이퍼 공정용 알루미나 정전척의 제작과 특성에 관한 연구 (A study on the Fabrication and Characterization of Alumina Electrostatic Chuck for Silicon Wafer Processing)

  • 정광진;박용균;이영섭;조동율;천희곤
    • 센서학회지
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    • 제8권6호
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    • pp.481-486
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    • 1999
  • Alumina electrostatic chucks for silicon wafer process with wide range of electrical resistivity were fabricated by controlling the amount of $TiO_2$ addition(0, 1.3, 2.0, 2.8 wt%). The dependence of electrostatic force on applied voltage, temperature and humidity was investigated. In addition, response characteristics on applied voltage and relationship between electrical resistivity and electrostatic force characteristics such as Coulomb force and Johnsen-Rahbeck force were discussed.

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토양유실 저감을 위한 지표피복 저감효과 변수 결정 및 SWAT 모형 유역단위 효과 분석 (Determination of Model Parameters of Surface Cover Materials in Evaluation of Sediment Reduction and Its Effects at Watershed Scale using SWAT)

  • 금동혁;장춘화;신민환;최중대;김범철;정교철;원철희;임경재
    • 한국물환경학회지
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    • 제28권6호
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    • pp.923-932
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    • 2012
  • The purpose of this study was to determine parameters of surface cover materials and evaluation the effects on runoff and sediment reductions with rice straw mat with PAM at watershed scale using the SWAT model. In this study, 1) regression equation of CN for rice straw mat + PAM using SCS curve number method was developed, 2) the USLE P factor, being able to reflect simulation of rice straw mat + PAM in the agricultural field, was estimated for various slope scenarios with VFSMOD-w. Then regression equation for CN and USLE P factor were used as input data in the SWAT model. Assuming rice straw mat + PAM is applied to radish and potato fields, occupying 24% of agricultural fields at the study watershed. Result of direct runoff without rice straw mat + PAM was $65,964,368\;m^3,$ with rice straw mat + PAM, direct runoff was $65,637,336\;m^3$, $327,031.8\;m^3$ reductions compared without it. Also, result of sediment without rice straw mat + PAM was 163,531 ton, with rice straw mat + PAM, sediment was 84,779 ton, 78,752 ton reduction compared without it. This analysis showed that about 48% sediment reductions would be expected with rice straw mat + PAM. As shown in this study, rice straw mat + PAM would be used as an efficient site-specific BMPs to reduce runoff and sediment discharge from field.

지반의 상호작용을 고려한 전면기초의 해석기법 개발 (Development of Analysis Method for Mat Foundations Considering Coupled Soil Springs)

  • 이재환;정상섬;이준규
    • 한국지반공학회논문집
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    • 제30권12호
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    • pp.41-49
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    • 2014
  • 본 연구에서는 기존 근사해석 프로그램의 간편함을 유지하면서 보다 정밀성이 확보된 3차원 근사해석기법(YS-MAT)을 개발하였다. 전면기초는 6개의 자유도를 가진 평면쉘 요소로 모델링하여 기초의 연성거동을 고려할 수 있도록 하였으며, 지반스프링의 상호작용을 고려할 수 있도록 하였다. 기존의 해석기법, 유한요소해석 및 현장계측값과의 비교 분석 결과, 본 해석기법이 대단면 전면기초의 침하 거동을 비교적 정확히 산정하는 것으로 판단되며, 이러한 검증을 토대로 실제 전면기초 예비설계에 적용 가능함을 확인할 수 있었다.

쇄석Mat를 이용한 수평배수공법 설계사례 (Design Example of Gravel Mat for Horizontal Drains)

  • 정경한;이영근;이시우;김재성;김병탁
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2005년도 지반공학 공동 학술발표회
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    • pp.174-187
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    • 2005
  • Recently, because of environment, cost, supply and demand factors, though applying sea-sand as horizontal drains is getting difficult that usage of Gravel has been growing in large size of construction sites, Study on engineering properties and behavior characteristics of Gravel stratum is not thoroughgoing enough. We have applied Gravel Mat as the horizontal drains in O O construction site. We also conducted several field tests such as Material property test, Geosynthetics damage test with Repeated load, Discharge capacity test performed by inflow of upper soil and In-situ PBD Penetration test to review the application of Gravel Mat. Test results show that Gravel Mat is not only advantageous in Trafficability and Water drainage by Consolidation due to its great Internal friction angle and Permeability, but also easy to penetrate with Mandrel and has great discharge capacity and guarantee of the stability against geosynthetics damage at the same time. With these benefits Gravel Mat shows great application in fields.

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