• Proceedings of the Korean Institute of Building Construction Conference
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• 2018.05a
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• pp.255-256
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• 2018

In this study, to confirm the performance of gel type urethane (hereinafter referred to as urethane gel) developed for the purpose of securing waterproof stability after application by increasing compatibility with asphalt mastic film material, general urethane coating material and adhesion. As a result, gel type urethane showed "all right" in fatigue resistance test.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.10
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• pp.1500-1509
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• 2014

The object of this study was to investigate the inhibitory effects of chios mastic gum (MG) on gastric acid secretion in an ethanol-induced SD rat model and primary parietal cells. Rats were randomly divided into four groups: Vehicle (normal group), Control (treated with ethanol), MG50 (treated with ethanol and mastic gum at 50 mg/kg b.w), MG100 (treated with ethanol and mastic gum at 100 mg/kg b.w). Groups treated with both MG50 and MG100 showed attenuation of gastric mucosal injury, sub-epithelial loss, hemorrhaging, and gastric juice secretion. We also examined the acidity of gastric juice during gastric injury. Oral administration of both MG50 and MG100 significantly decreased acidity of gastric juice by % and %, respectively. To examine the stimulatory factors related to gastric acid secretion, mRNA expression levels of H2r, M3r, CCK2r, and $H^+/K^+$ ATPase were measured by real-time PCR. Compared with a vehicle group, mRNA expression levels of H2r, CCK2r, and $H^+/K^+$ ATPase clearly increased in the control group. However, levels of H2r, CCK2r, and $H^+/K^+$ ATPase slightly but significantly decreased in MG-treated groups compared with control. Blood level of histamine significantly decreased in MG-treated groups, which indicates the involvement of MG on in histamine-related acid secretion. To identify the mode of action of MG in regulating histamine-related pathways, intracellular level of cAMP and mRNA levels of H2r, M3r, CCK2r, and $H^+/K^+$ ATPase were measured in primary parietal cells. While mRNA levels of M3r and CCK2r remained unchanged, levels of H2r and $H^+/K^+$ ATPase significantly decreased upon MG treatment. Subsequently, intracellular levels of cAMP decreased. These results suggest that mastic gum has the ability to inhibit gastric acid secretion by regulating a histamine-related pathway.

• International Journal of Oral Biology
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• v.36 no.1
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• pp.13-21
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• 2011

Chios gum mastic (CGM) is produced from Pistiacia lentiscus L var chia, which grows only on Chios Island in Greece. CGM is a kind of resin extracted from the stem and leaves, has been used for many centuries in many Mediterranean countries as a dietary supplement and folk medicine for stomach and duodenal ulcers. CGM is known to induce cell cycle arrest and apoptosis in some cancer cells. This study was undertaken to investigate the alteration of the cell cycle and induction of apoptosis following CGM treatment of HL-60 cells. The viability of the HL-60 cells was assessed using the MTT assay. Hoechst staining and DNA electrophoresis were employed to detect HL-60 cells undergoing apoptosis. Western blotting, immunocytochemistry, confocal microscopy, FACScan flow cytometry, MMP activity and proteasome activity analyses were also employed. CGM treatment of HL-60 cells was found to result in a dose- and time-dependent decrease in cell viability and apoptotic cell death. Tested HL-60 cells showed a variety of apoptotic manifestations and induced the downregulation of G1 cell cycle-related proteins. Taken collectively, our present findings demonstrate that CGM strongly induces G1 cell cycle arrest via the modulation of cell cycle-related proteins, and also apoptosis via proteasome, mitochondrial and caspase cascades in HL-60 cells. Hence, we provide evidence that a natural product, CGM could be considered as a novel therapeutic for human leukemia.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.35 no.2
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• pp.125-130
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• 2009

For many years, intermaxillary fixation using arch bar has been operated in treatment of mandibular fracture patients. But it has many complications including injury of operators and assistants cause by wire, inflammation of periodontium. For that reasons alternatives are required; osteosynthesis technique using mini plate, intermaxillary fixation using IMF screws have been available. Treatment by arch bar fixation, however, is still valuable to treat craniomaxillary fracture patients. The purpose of this study is to know effect arch bar on periodontium and influence gingival gel on periodontium applied by arch bar. 40 mandibular fracture patients are monitored. 30 patients were applied by arch bar, 10 patients were not. And the former were classified by 3 categories; Nano vitamin and Mastic gel were applied to 10 patients respectively and any gingival gel was not used to 10 patients. Clinical attachment level, bleeding on probing and periodontal depth of each group were measured and compared before operation and on 2 weeks and 6 weeks after operation. Mann-Whitney U test was used to analyze result which leads to this conclusion. 1. Whether arch bar is applied or not, treatment of mandlbular fracture gave rise to gingivitis, but 6 weeks after operation, gingivitis is restored to the same level as the state before operation. 2. More severe gingivitis appeared when arch bar is applied to mandibular fracture than when it is not. 3. Both gingival gel used in this study can reduce gingivitis which can be caused by arch bar. 4. In this study, Mastic gel is more effective for prevent gingival inflammation cause by arch bar than nano vitamin. In regard to this result, gingivitis is considered to be available because it is reversible and does not induce periodontal disease. Gingival gel is regarded to be helpful for patients applied by arch bar to feel less discomfort.

• International Journal of Oral Biology
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• v.38 no.3
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• pp.101-110
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• 2013

We investigated the synergistic apoptotic effects of co-treatments with Chios gum mastic (CGM) and eugenol on G361 human melanoma cells. An MTT assay was conducted to investigate whether this co-treatment efficiently reduces the viability of G361 cells compared with each single treatment. The induction and augmentation of apoptosis were confirmed by DNA electrophoresis, Hoechst staining, and analyses of DNA hypoploidy. Western blot analysis and immunofluorescent staining were also performed to evaluate expression and translocation of apoptosis-related proteins following CGM and eugenol co-treatment. Proteasome activity and mitochondrial membrane potential (MMP) changes were also assayed.The results indicated that the co-treatment of CGM and eugenol induces multiple pathways and processes associated with an apoptotic response in G361 cells. These include nuclear condensation, DNA fragmentation, a reduction in MMP and proteasome activity, an increase of Bax and decrease of Bcl-2, a decreased DNA content, cytochrome c release into the cytosol, the translocation of AIF and DFF40 (CAD) into the nucleus, and the activation of caspase-9, caspase-7, caspase-3, PARP and DFF45 (ICAD). In contrast, separate treatments of $40{\mu}g/ml$ CGM or $300{\mu}M$ eugenol for 24 hours did not induce apoptosis. Our present data thus suggest that a combination therapy of CGM and eugenol is a potential treatment strategy for human melanoma.

• International Journal of Oral Biology
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• v.39 no.2
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• pp.65-73
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• 2014

Chios Gum Mastic (CGM) is a natural resin extracted from the leaves of Pistacia lentiscus, a plant endemic to the Greek island of Chios. It has been used by traditional healers, and it has antibacterial, antifungal properties, and therapeutic benefits for the skin. The CGM reduces the formation of dental plaque and bacterial growth in oral saliva, and recent studies have demonstrated the role of antioxidant activity of CGM. Although CGM has been widely investigated, its protective effect against oxidative-damage to keratinocytes, as well as the relationship between CGM and autophagy, has not been investigated. The aim of this study was to assess the protective effect of CGM against $H_2O_2$-induced oxidative stress and to evaluate the autophagic features induced by CGM in human keratinocytes. The pretreatment with CGM significantly reduced apoptosis in $H_2O_2$-exposed HaCaT cells. It promoted the degradation of caspase-3, caspase-8, and caspase-9; and it induced the formation of the processed PARP. The treatment with CGM caused an increase in vesicle formation compared to control group. The level of p62 was reduced and the conversion of LC3-I to LC3-II was increased in CGM treated HaCaT cells. Also, the treatment with CGM increased cleavage of ATG5-ATG12 complex. In summary, CGM helps the cells to survive under stressful conditions by preventing apoptosis and enhancing autophagy. Besides, the present investigation provides evidence to support the antioxidant potential of CGM in vitro and opens up a new horizon for future experiments.

• Journal of the Korea Institute of Building Construction
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• v.18 no.4
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• pp.337-345
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• 2018

In this study, observations of oil leakage samples taken from the actual site were performed to identify the causes of the oil leakage phenomenon. As a result, the separation of the material components was determined as the main cause of the oil leakage phenomenon based on the changes in the surface conditions, and verification of this was conducted. The evaluation results confirmed that the filler component of the asphalt mastic subsided with the lapse of the settling time, and that the difference ratio of the filler contents of the upper and lower specimens was up to 23.8% after day 28. Based on these results, a hypothesis on the oil leakage mechanism of asphalt mastic was established, and then modeling of the entire process of oil leakage was performed.

• The Journal of the Korean dental association
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• v.17 no.10 s.125
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• pp.769-772
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• 1979

Child female 11 years old fractures of mandible in symphysis and left condyle head, conservative treated intermaxillary wiring (Rubber bands elastic) 8 days after mandible movement fractures healed. Patient has practically normal mastic atory function and no complaints.

• International Journal of Highway Engineering
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• v.15 no.1
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• pp.103-110
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• 2013

PURPOSES: The objective of this study is to analyze and evaluate the behavior of orthotropic steel bridge deck pavement using three-dimensional finite element analysis and full-scale wheel load testing. METHODS: Since the layer thickness and material properties used in the bridge deck pavement are different from its condition, it is very difficult to measure and access the behavior of bridge deck pavement in the field. To solve this problem, the full-scale wheel load testing was conducted on the PSMA/Mastic bridge deck pavement and the deflection of bridge deck and horizontal tensile strain on top of pavement were measured under the loading condition. Three-dimensional finite element analysis was conducted to predict the behavior of bridge deck pavement and the predicted deflection and tensile strain values are compared with measured values from the wheel loading testing. RESULTS: Test results showed that the predicted deflections are 10% lower than measured ones and the error between predicted and measured horizontal tensile strain values is less than 2% in the critical location. CONCLUSIONS: The fact indicates that the proposed the analysis is found to be accurate for estimating the behavior of bridge deck pavements.

• International Journal of Highway Engineering
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• v.18 no.1
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• pp.91-97
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• 2016

PURPOSES : The objective of this paper is to develop a pavement performance model based on the Bayesian algorithm, and compare the measured and predicted performance data. METHODS : In this paper, several pavement types such as SMA (stone mastic asphalt), PSMA (polymer-modified stone mastic asphalt), PMA (polymer-modified asphalt), SBS (styrene-butadiene-styrene) modified asphalt, and DGA (dense-graded asphalt) are modeled in terms of the performance evaluation of pavement structures, using the Bayesian algorithm. RESULTS : From case studies related to the performance model development, the statistical parameters of the mean value and standard deviation can be obtained through the Bayesian algorithm, using the initial performance data of two different pavement cases. Furthermore, an accurate performance model can be developed, based on the comparison between the measured and predicted performance data. CONCLUSIONS : Based on the results of the case studies, it is concluded that the determined coefficients of the nonlinear performance models can be used to accurately predict the long-term performance behaviors of DGA and modified asphalt concrete pavements. In addition, the developed models were evaluated through comparison studies between the initial measurement and prediction data, as well as between the final measurement and prediction data. In the model development, the initial measured data were used.