• Title/Summary/Keyword: mass-propagation

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Change of Rooting Potential as Affected by Cutting Time in Corylopsis coreana (히어리(Corylopsis coreana)에 있어서 삽목시기에 따른 발근력의 변화)

  • Kim, Jae Chang;Jeong, Jeong Hag
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.4
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    • pp.262-265
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    • 2009
  • This study was conducted to find out a proper cutting time as related with a growth pattern for the mass propagation of Corylopsis coreana, which is one of the Korean endemic plant. New shoots began to grow in early April soon after end of blooming time and grew rapidly until the end of June. Shoot growth became dull after June and stopped in early September, showing a typical primary growth curve of temperate deciduous trees which show only one flush of growth throughout an annual growth cycle. The moisture content of shoot was the highest in an early growing season of May, and decreased with shoot maturity. Moisture content of cuttings taken on June 20 was 71.2%. Rooting was better in softwood cuttings than in hardwood cuttings. The highest value of rooting percentage was obtained in cuttings taken on June 20.

Model test on slope deformation and failure caused by transition from open-pit to underground mining

  • Zhang, Bin;Wang, Hanxun;Huang, Jie;Xu, Nengxiong
    • Geomechanics and Engineering
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    • v.19 no.2
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    • pp.167-178
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    • 2019
  • Open-pit (OP) and underground (UG) mining are usually used to exploit shallow and deep ore deposits, respectively. When mine deposit starts from shallow subsurface and extends to a great depth, sequential use of OP and UG mining is an efficient and economical way to maintain mining productivity. However, a transition from OP to UG mining could induce significant rock movements that cause the slope instability of the open pit. Based on Yanqianshan Iron Mine, which was in the transition from OP to UG mining, a large-scale two-dimensional (2D) model test was built according to the similar theory. Thereafter, the UG mining was carried out to mimic the process of transition from OP to UG mining to disclose the triggered rock movement as well as to assess the associated slope instability. By jointly using three-dimensional (3D) laser scanning, distributed fiber optics, and digital photogrammetry measurement, the deformations, movements and strains of the rock slope during mining were monitored. The obtained data showed that the transition from OP to UG mining led to significant slope movements and deformations that can trigger catastrophic slope failure. The progressive movement of the slope could be divided into three stages: onset of micro-fracture, propagation of tensile cracks, and the overturning and/or sliding of slopes. The failure mode depended on the orientation of structural joints of the rock mass as well as the formation of tension cracks. This study also proved that these non-contact monitoring technologies were valid methods to acquire the interior strain and external deformation with high precision.

Ignition Characteristics of Combustion Chamber with $LO_X$ Lead Cyclogram for Liquid Rocket Engine (액체로켓엔진 연소기 산화제 선공급 Cyclogram에 의한 점화특성)

  • Han, Yeoung-Min;Kim, Jong-Gyu;Lee, Kwang-Jin;Lim, Byoung-Jik;Ahn, Kyu-Bok;Kim, Mun-Ki;Seo, Seong-Hhyeon;Choi, Hwan-Seok
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2008.11a
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    • pp.137-142
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    • 2008
  • Ignition characteristics of combustion chamber with LOx lead cyclogram for liquid rocket engine were described. The combustion chamber has chamber pressure of 60 bar, propellant mass flow rate of 89 kg/s, and nozzle expansion of 12. Cold flow test to determine the filling time of propellant for cyclogram with LOx lead supply, ignition test to check the ability to ignite starting fuel from the ignitor, low pressure combustion test to check the propagation of flame into main fuel-oxidizer mixture from starting fuel and the main combustion stage, and design point combustion test to check the combustion performance were performed. Ignition and combustion tests with LOx lead supply were successfully performed and the stable cyclogram of start sequence for combustion chamber was developed.

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Large-Scale Production of Cronobacter sakazakii Bacteriophage Φ CS01 in Bioreactors via a Two-Stage Self-Cycling Process

  • Lee, Jin-Sun;Kim, Gyeong-Hwuii;Kim, Jaegon;Lim, Tae-Hyun;Yoon, Yong Won;Yoon, Sung-Sik
    • Journal of Microbiology and Biotechnology
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    • v.31 no.10
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    • pp.1430-1437
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    • 2021
  • Cronobacter sakazakii is an opportunistic pathogenic bacterium found in powdered infant formula and is fatal to neonates. Antibiotic resistance has emerged owing to overuse of antibiotics. Therefore, demand for high-yield bacteriophages as an alternative to antibiotics has increased. Accordingly, we developed a modified mass-production method for bacteriophages by introducing a two-stage self-cycling (TSSC) process, which yielded high-concentration bacteriophage solutions by replenishing the nutritional medium at the beginning of each process, without additional challenge. pH of the culture medium was monitored in real-time during C. sakazakii growth and bacteriophage CS01 propagation, and the changes in various parameters were assessed. The pH of the culture medium dropped to 5.8 when the host bacteria reached the early log phase (OD540 = 0.3). After challenge, it decreased to 4.65 and then recovered to 4.94; therefore, we set the optimum pH to challenge the phage at 5.8 and that to harvest the phage at 4.94. We then compared phage production during the TSSC process in jar-type bioreactors and the batch culture process in shaker flasks. In the same volume of LB medium, the concentration of the phage titer solution obtained with the TSSC process was 24 times higher than that obtained with the batch culture process. Moreover, we stably obtained high concentrations of bacteriophage solutions for three cycles with the TSSC process. Overall, this modified TSSC process could simplify large-scale production of bacteriophage CS01 and reduce the unit cost of phage titer solution. These results could contribute to curing infants infected with antibiotic-resistant C. sakazakii.

Stochastic analysis of the rocking vulnerability of irregular anchored rigid bodies: application to soils of Mexico City

  • Ramos, Salvador;Arredondo, Cesar;Reinoso, Eduardo;Leonardo-Suarez, Miguel;Torres, Marco A.
    • Earthquakes and Structures
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    • v.20 no.1
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    • pp.71-86
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    • 2021
  • This paper focuses on the development and assessment of the expected damage for the rocking response of rigid anchored blocks, with irregular geometry and non-uniform mass distribution, considering the site conditions and the seismicity of Mexico City. The non-linear behavior of the restrainers is incorporated to evaluate the pure tension and tension-shear failure mechanisms. A probabilistic framework is performed covering a wide range of block sizes, slenderness ratios and eccentricities using physics-based ground motion simulation. In order to incorporate the uncertainties related to the propagation of far-field earthquakes with a significant contribution to the seismic hazard at study sites, it was simulated a set of scenarios using a stochastic summation methods of small-earthquakes records, considered as Empirical Green's Function (EGFs). As Engineering Demand Parameter (EDP), the absolute value of the maximum block rotation normalized by the body slenderness, as a function of the peak ground acceleration (PGA) is adopted. The results show that anchorages are more efficient for blocks with slenderness ratio between two and three, while slenderness above four provide a better stability when they are not restrained. Besides, there is a range of peak intensities where anchored blocks located in soft soils are less vulnerable with respect to those located in firm soils. The procedure used in here allows to take decisions about risk, reliability and resilience assessment of different types of contents, and it is easily adaptable to other seismic environments.

Production performances and antioxidant activities of laying hens fed Aspergillus oryzae and phytase co-fermented wheat bran

  • Huang, Chung Ming;Chuang, Wen Yang;Lin, Wei Chih;Lin, Li Jen;Chang, Sheng Chang;Lee, Tzu Tai
    • Animal Bioscience
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    • v.34 no.3_spc
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    • pp.371-384
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    • 2021
  • Objective: Wheat bran (WB) was co-fermented with Aspergillus oryzae and phytase (Phy) to determine whether co-fermentation improve WB phosphorus and fiber utilization in Isa-brown layers. Methods: A total of 112 Isa brown layer were randomly divided into 7 treatments with 8 replicates per a treatment and 2 hens per a replicate. The treatments included basal diet (control), basal diet supplemented with 250 unit/kg Phy (control+Phy), diet with 10% WB (10% WB), diet with 5% WB and 250 unit/kg Phy (5% WB+Phy) diet with 10% WB and 250 unit/kg Phy (10% WB+Phy), diet with 5% fermented WB supplemented with molasses and phy (PCFWH) and 125 unit/kg Phy (5% PCFWH), and diet with 10% PCFWH (10% PCFWH). The intestinal microbial population, intestinal morphology, serum antioxidant enzyme activities, and excreta phosphorus content were assessed. Results: In PCFWH, spore counts, protease activity, xylanase activity, and ferulic acid were 8.50 log/g dry matter (DM), 190 unit/g DM, 120 unit/g DM, and 127 ㎍/g, respectively. Xylobiose and xylotriose were released in PCFWH, while they were not detectable in WB. Antioxidant capacity was also enhanced in PCFWH compared to WB. The 10% WB+Phy and 10% PCFWH groups produced higher egg mass, but hens fed 5% WB+Phy had the lowest amount of feed intake. Eggs from 10% PCFWH had better eggshell weight, eggshell strength, and eggshell thickness. Birds fed with 10% PCFWH also had higher serum superoxide dismutase and catalase activities. Compare to control, 10% PCFWH significantly reduced excreta phosphorus content. Conclusion: Diet inclusion of 10% PCFWH improved egg quality, antioxidant status, and excreta phosphorus content of laying hens.

Evaluation of Internal Blast Overpressures in Test Rooms of Elcetric Vehicles Battery with Pressure Relief Vents (압력배출구를 설치한 전동화 차량 배터리 시험실의 내부 폭압 평가)

  • Pang, Seungki;Shin, Jinwon;Jeong, Hyunjin
    • Journal of the Korean Society for Geothermal and Hydrothermal Energy
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    • v.18 no.3
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    • pp.7-18
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    • 2022
  • Secondary batteries used in electric vehicles have a potential risk of ignition and explosion. Various safety measures are being taken to prevent these risks. A numerical study was performed using a computational fluid dynamics code on the cases where pressure relief vents that can reduce the blast overpressures of batteries were installed in the through-compression test room, short-circuit drop test room, combustion test room, and immersion test room in facilities rleated to battery used in electric vehicles. This study was conducted using the weight of TNT equivalent to the energy release from the battery, where the the thermal runaway energy was set to 324,000 kJ for the capacity of the lithium-ion battery was 90 kWh and the state of charge (SOC) of the battery of 100%. The explosion energy of TNT (△HTNT) generally has a range of 4,437 to 4,765 kJ/kg, and a value of 4,500 kJ/kg was thus used in this study. The dimensionless explosion efficiency coefficient was defined as 15% assuming the most unfavorable condition, and the TNT equivalent mass was calculated to be 11 kg. The internal explosion generated in a test room shows the very complex propagation behavior of blast waves. The shock wave generated after the explosion creates reflected shock waves on all inner surfaces. If the internally reflected shock waves are not effectively released to the outside, the overpressures inside are increased or maintained due to the continuous reflection and superposition from the inside for a long time. Blast simulations for internal explosion targeting four test rooms with pressure relief vents installed were herein conducted. It was found that that the maximum blast overpressure of 34.69 bar occurred on the rear wall of the immersion test room, and the smallest blast overpressure was calculated to be 3.58 bar on the side wall of the short-circuit drop test room.

Inhibitory Effects of Pepper Mild Mottle Virus Infection by Supernatants of Five Bacterial Cultures in Capsicum annuum L.

  • Venkata Subba Reddy, Gangireddygari;In-Sook, Cho;Sena, Choi;Ju-Yeon, Yoon
    • The Plant Pathology Journal
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    • v.38 no.6
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    • pp.646-655
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    • 2022
  • Pepper mild mottle virus (PMMoV), one of the most prevalent viruses in chili pepper (Capsicum annuum L.) is a non-enveloped, rod-shaped, single-stranded positive-sense RNA virus classified in the genus Tobamovirus. The supernatants of five bacterial cultures (Pseudomonas putida [PP], Bacillus licheniformis [BLI], P. fluorescens [PF], Serratia marcescens [SER], and B. amyloliquifaciens [BA]) were analyzed to find novel antiviral agents to PMMoV in chili pepper. Foliar spraying with supernatants (1:1, v/v) obtained from Luria-Bertani broth cultures of PP, BLI, PF, SER, and BA inhibited PMMoV infection of chili pepper if applied before the PMMoV inoculation. Double-antibody sandwich enzyme-linked immunosorbent assay showed that treatments of five supernatants resulted in 51-66% reductions in PMMoV accumulation in the treated chili pepper. To identify key compounds in supernatants of PP, BLI, PF, SER, and BA, the supernatants were subjected to gas chromatography-mass spectrometry. The 24 different types of compounds were identified from the supernatants of PP, BLI, PF, SER, and BA. The compounds vary from supernatants of one bacterial culture to another which includes simple compounds-alkanes, ketones, alcohols, and an aromatic ring containing compounds. The compounds triggered the inhibitory effect on PMMoV propagation in chili pepper plants. In conclusion, the cultures could be used to further conduct tissue culture and field trial experiments as potential bio-control agents.

Simulation of the fracture of heterogeneous rock masses based on the enriched numerical manifold method

  • Yuan Wang;Xinyu Liu;Lingfeng Zhou;Qi Dong
    • Geomechanics and Engineering
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    • v.34 no.6
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    • pp.683-696
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    • 2023
  • The destruction and fracture of rock masses are crucial components in engineering and there is an increasing demand for the study of the influence of rock mass heterogeneity on the safety of engineering projects. The numerical manifold method (NMM) has a unified solution format for continuous and discontinuous problems. In most NMM studies, material homogeneity has been assumed and despite this simplification, fracture mechanics remain complex and simulations are inefficient because of the complicated topology updating operations that are needed after crack propagation. These operations become computationally expensive especially in the cases of heterogeneous materials. In this study, a heterogeneous model algorithm based on stochastic theory was developed and introduced into the NMM. A new fracture algorithm was developed to simulate the rupture zone. The algorithm was validated for the examples of the four-point shear beam and semi-circular bend. Results show that the algorithm can efficiently simulate the rupture zone of heterogeneous rock masses. Heterogeneity has a powerful effect on the macroscopic failure characteristics and uniaxial compressive strength of rock masses. The peak strength of homogeneous material (with heterogeneity or standard deviation of 0) is 2.4 times that of heterogeneous material (with heterogeneity of 11.0). Moreover, the local distribution of parameter values can affect the configuration of rupture zones in rock masses. The local distribution also influences the peak value on the stress-strain curve and the residual strength. The post-peak stress-strain curve envelope from 60 random calculations can be used as an estimate of the strength of engineering rock masses.

TBM disc cutter ring type adaptability and rock-breaking efficiency: Numerical modeling and case study

  • Xiaokang Shao;Yusheng Jiang;Zongyuan Zhu;Zhiyong Yang;Zhenyong Wang;Jinguo Cheng;Quanwei Liu
    • Geomechanics and Engineering
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    • v.34 no.1
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    • pp.103-113
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    • 2023
  • This study focused on understanding the relationship between the design of a tunnel boring machine disc cutter ring and its rock-breaking efficiency, as well as the applicable conditions of different cutter ring types. The discrete element method was used to establish a numerical model of the rock-breaking process using disc cutters with different ring types to reveal the development of rock damage cracks and variation in cutter penetration load. The calculation results indicate that a sharp-edged (V-shaped) disc cutter penetrates a rock mass to a given depth with the lowest load, resulting in more intermediate cracks and few lateral cracks, which leads to difficulty in crack combination. Furthermore, the poor wear resistance of a conventional V-shaped cutter can lead to an exponential increase in the penetration load after cutter ring wear. In contrast, constant-cross-section (CCS) disc cutters have the highest quantity of crack extensions after penetrating rock, but also require the highest penetration loads. An arch-edged (U-shaped) disc cutter is more moderate than the aforementioned types with sufficient intermediate and lateral crack propagation after cutting into rock under a suitable penetration load. Additionally, we found that the cutter ring wedge angle and edge width heavily influence cutter rock-breaking efficiency and that a disc cutter with a 16 to 22 mm edge width and 20° to 30° wedge angle exhibits high performance. Compared to V-shaped and U-shaped cutters, the CCS cutter is more suitable for soft or medium-strength rocks, where the penetration load is relatively small. Additionally, two typical case studies were selected to verify that replacing a CCS cutter with a U-shaped or optimized V-shaped disc cutter can increase cutting efficiency when encountering hard rocks.