• 제목/요약/키워드: mass-propagation

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Identification of Retinol-binding Protein Produced by Caprine Endometrium during Periattachment Period of Early Pregnancy

  • Liu, K.H.;Huang, J.C.;Lin, J.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.12
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    • pp.1708-1713
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    • 2002
  • Endometrial explants obtained from does between days 13 and 21 of pregnancy were cultured in a modified minimum essential medium in the presence of [$^35S$]methionine and [$^3H$]-leucine. Proteins synthesized and secreted into medium were analyzed by fluorography of two-dimensional polyacrylamide gel electrophoresis and fluorography. No marked qualitative changes in patterns of protein production by caprine endometrium between days 13-21 of pregnancy. At least 11 proteins showed consistently a clear spot or a grouping of spots with characteristic location on two-dimensional gels. A major low molecular weight protein consisted of two major isoforms (pI 5.3-6.0) of similar molecular mass (21 kDa). Limited N-terminal sequence analysis of these two isoforms showed that the protein had complete homology with bovine placental and plasma retinol-binding protein (RBP) over the first 20 amino acids. Through use of the antiserum raised against bovine placental RBP, immunoreactive RBP was detected in cultures conditioned by uterine explants prepared at days 13, 15 and 21 of pregnancy. In the present study, proteins synthesized and secreted by caprine endometrium during periattachment period of early pregnancy were characterized. The pregnant endometrium secreted a number of neutral-to-acidic proteins which constituted, in part, the histotroph. A vitamin A-transport protein, RBP, was identified in cultures conditioned by endometrium of days 13-21 of pregnancy. The uterine endometrium is the only source of retinol for embryonic tissues. The uterine RBP appears to transport retinol locally toward embryonic tissues. Secretion of RBP by caprine endometrium of days 13, 15 and 21 of pregnancy suggested that retinol played an important role in conceptus development during periattachment period of early pregnancy.

A Numerical Study on the Fire Behavior Phenomena in a Special Fire Protection Compartment (특수 방호공간에서 가상화재의 발생으로 인한 화재거동에 관한 수치적 연구)

  • Kim, Tae-Kuk;Son, Bong-Sei
    • Journal of the Korean Society of Hazard Mitigation
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    • v.1 no.1 s.1
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    • pp.157-163
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    • 2001
  • The objective of the present study is to predict the characteristics of the fire and smoke propagations in a clean room. Numerical calculations have been performed by using the finite volume method to obtain temperature and velocity distributions in the clean room. In odor to account for the turbulent flow characteristics, the standard $k-{\varepsilon}$ model is used. From this study, it was found that the fire propagation could be fully developed only after 150 seconds when the ventilation system in the clean room was off. And the smoke mass fraction showed a similar distribution as the gas temperature. Since the simulated fire was proceeded up to $20{\sim}30%$ of the room within 60 seconds. it could be recommended that the occupants should be evacuated from the room within 30 seconds.

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Shoot multiplication kinetics and hyperhydric status of regenerated shoots of gladiolus in agar-solidified and matrix-supported liquid cultures

  • Gupta, S. Dutta;Prasad, V.S.S.
    • Plant Biotechnology Reports
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    • v.4 no.1
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    • pp.85-94
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    • 2010
  • In vitro shoot regeneration of gladiolus in three different culture systems, viz., semi-solid agar (AS), membrane raft (MR), and duroplast foam liquid (DF) cultures was evaluated following the kinetics of shoot multiplication and hyperhydricity at optimized growth regulator combinations. Compared to the AS system, matrixsupported liquid cultures enhanced shoot multiplication. The peak of shoot multiplication rate was attained at 18 days of incubation in the MR and DF systems, whereas the maximum rate in the AS system was attained at 21 days. An early decline in acceleration trend was observed in liquid cultures than the AS culture. The hyperhydric status of the regenerated shoots in the different culture systems was assessed in terms of stomatal attributes and antioxidative status. Stomatal behavior appeared to be normal in the AS and MR systems. However, structural anomaly of stomata such as large, round shaped guard cells with damage in bordering regions of stomatal pores was pronounced in the DF system along with a relatively higher $K^+$ ion concentration than in the AS and MR systems. Antioxidative status of regenerated shoots was comparable in the AS and MR systems, while a higher incidence of oxidative damages of lipid membrane as evidenced from malondialdehyde and ascorbate content was observed in the DF system. Higher oxidative stress in the DF system was also apparent by elevated activities of superoxide dismutase, ascorbate peroxidase, and catalase. Among the three culture systems, liquid culture with MR resulted in maximum shoot multiplication with little or no symptoms of hyperhydricity. Shoots in the DF system were more prone to hyperhydricity than those in the AS and MR systems. The use of matrix support such as membrane raft as an interface between liquid medium and propagating tissue could be an effective means for rapid and efficient mass propagation with little or no symptoms of hyperhydricity.

Assessment of Blast-induced Vibration Using Dynamic Distinct Element Analysis (불연속체 동해석 기법을 이용한 발파진동 영향평가)

  • Park, Byung-Ki;Jeon, Seokwon;Park, Gwang-Jun;Do, Deog-Soo;Kim, Tae-Hoon;Jung, Du-Seop
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.15 no.12 s.105
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    • pp.1389-1397
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    • 2005
  • Since blast-induced vibration may cause serious problem to the rock mass as well as the nearby structures, the prediction of blast-induced nitration and the stability evaluation must be performed before blasting activities. Dynamic analysis has been increased recently in order to analyze the effect of the blast-Induced vibration. Most of the previous studies, however, were based on the continuum analysis unable to consider rock joints which significantly affect the wave propagation and attenuation characteristics. They also adopted pressure corves estimated tv theoretical or empirical equations as input detonation load, thus there were very difficult to reflect the characteristics of propagating media. In this study, therefore, we suggested a dynamic distinct element analysis technique which uses velocity waveform obtained from a test blast as an input detonation load. A distinct element program, UDEC was used to consider the effect of rock joints. In order to verify the validity of proposed method, the test blast was simulated. The predicted results from the proposed method showed a good agreement with the measured vibration data from the test blast. Through the dynamic numerical modelling on the planned road tunnel and slope, we evaluated the effect of blast-induced nitration and the stability of rock slope.

Selection of the high yield capacity of Hwangchil lacquer and identification of aromatic components in essential oil of Dendropanax morbifera Lev. (황칠수액 분비 우수개체 선발 및 방향성 정유성분 조사)

  • Ahn, Jun-Cheul;Kim, Min-Young;Kim, Ok-Tae;Kim, Kwang-Soo;Kim, Sung-Ho;Kim, Sea-Hyun;Hwang, Baik
    • Korean Journal of Medicinal Crop Science
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    • v.10 no.2
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    • pp.126-131
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    • 2002
  • We investigated the high yield capacity of Hwangchil lacquer for selection and identification of major aromatic components of the selected trees as well. The Hwangchil lacquer showed the difference yields by different habitat, tree ages and individual character. The selected trees showed high yield capacity of Hwangchil lacquer. We also investigated the essential oil contents and its the main components to analysis the potent of mass propagation. The major compounds were 1,6-atadiene-3-ol., ${\alpha}-terpinene,\;{\alpha}-cubebene,\;{\alpha}-ylangene,\;{\alpha}-copane,\;{\beta}-elemene,\;germacrene-D,\;{\beta}-selinene,\;{\alpha}-selinene,\;{\delta}-cadinene,{\gamma}-cardinene$, germacrene B, germacrene D-4-ol. The most principle component was germacrene D among them respectively. However, the difference of relative content ratio of each major compound was showed by individuals, population and native areas in the selected trees.

An Experimental Study on Crack Propagation in KURT Granite using Acoustic Emission (음향방출기법을 이용한 KURT 화강암의 균열 발생 특성에 관한 실험적 연구)

  • Lee, Kyung-Soo;Kim, Jin-Seop;Choi, Jong-Won;Lee, Chang-Soo
    • The Journal of Engineering Geology
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    • v.21 no.4
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    • pp.295-304
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    • 2011
  • The first step in improving our understanding of uncertainties suclt as rock mass strength parameters and deformation modulus in rock masses around high-level radioactive waste disposal repositories, for improved safety, is to study the process of crack development in intact rock. Therefore, in this study, the fracture process and crack development were examined in samples of KURT granite taken from the KAERI Underground Research Tunnel (KURT), based on acoustic emission (AE) and moment tensor analysis. The results show that crack initiation, coalescence, and unstable crack occurred at rock uniaxial compressive strengths of 0.45, 0.73, and 0.84, respectively. In addition, moment tensor analysis indicated that during the early stage of loading, tensile cracks were predominant. With increasing applied stress, the number of shear cracks gradually increased. When the applied stress exceeded the stress level required for crack damage, unstable shear cracks which directly result in failure of the rock were generated along the failure plane.

Development of Culture System for Masspropagation and Acclimatization of Tissue Cultured Plantlets (유식물체 증식.순화용 배양시스템 개발)

  • Han, K.S.;Heo, J.W.;Kim, S.C.;Lee, Y.B.;Kim, S.C.;Im, D.H.;Choi, H.G.
    • Journal of Biosystems Engineering
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    • v.32 no.2 s.121
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    • pp.109-114
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    • 2007
  • In mass production of seed-potato plantlets, the processes for in vitro propagation and ex vitro acclimatization with a high cost should be improved by a culture system with environmental control using scaled-up culture vessels. The experiment was conducted to design a hydroponic culture system for enhancement of growth and development of seed-potato (Solanum tuberosum) plantlets cultured under photoautotrophic (without sugar in culture medium) conditions with controlled light intensity and ventilation rate. The culture system was consisted of scaled-up culture vessels, ventilation pipes, a multi-cell tray and an environmental control system (ECS) for optimum controlling in temperature, light intensity, ventilation rate, and culture-medium supply. Growth and development of the plantlets was significantly increased under the ECS compared with a conventional culture system (CCS) of photomixotrophic culture (with sugar in culture medium) using small scale vessels. For 21 days, leaf area of the plantlets was expanded more than 2 times, and number of internodes also approximately 4 times greate. under the ECS. In addition, the photoautotrophic growth in sweetpotato (Ipomoea batatas) and chrysanthemum (Chrysanthemum morifolium) plantlets was greater more than 2 times compared with the CCS.

Medium Composition Affecting In Vitro Plant Regeneration and Acclimation of Pteris cretica 'Wilsonii' (Pteris cretica 'Wilsonii'의 기내 식물체 재생에 미치는 배지구성물질의 영향 및 기외순화)

  • Shin, So-Lim;Lee, Cheol-Hee
    • Korean Journal of Plant Resources
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    • v.22 no.5
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    • pp.394-402
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    • 2009
  • Adventitious shoots were induced from pinnae, petiole and rhizome in Pteris cretica 'Wilsonii' in order to develop the efficient mass propagation method, using in vitro culture. Only homogenized rhizome segments could regenerate young sporophytes. Efficient regeneration of multiple shoots was obtained on the one-eighth strength MS medium containing 1% sucrose, and $50mg{\cdot}L^{-1}$ $NaH_2PO_4$. To achieve higher rate of regeneration from rhizome segments, rhizome segments were exposed to growth regulators for 2 months and then subcultured on hormone-free medium. The greatest shoot regeneration was obtained by $1{\mu}M$ kinetin with $5{\mu}M$ NAA. BA was effective in formation of GGB (kind of meristems), but they showed low shoot regeneration rate. Plants obtained from present experiments were transplanted to examine good environmental conditions for acclimation. Juvenile plants obtained by the one-eighth strength MS medium showed highest survival rate and vigorous growth at the seedling stage.

A Study on the Burning Characteristics of Interior Boards and Louvers (내장용 판재 및 루버의 연소발열특성 연구)

  • Nam, Dong-Gun;Kim, Sung-Chan
    • Fire Science and Engineering
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    • v.28 no.2
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    • pp.34-39
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    • 2014
  • It is necessary to enhance its availability and reliability of performance based fire design of building with various type of database such as experiments, survey and fire properties and so on. In order to utilize to the performance based fire design, the present study has been performed a series of experiments to investigate the burning characteristics of building materials for two types of interior board and three types of interior louver. The burning test has been also conducted for different thickness because it may show different characteristics of burning behavior such as flame spread rate and flame propagation time. The result shows that the effective heat release per unit mass of interior materials were almost constant with 15.3~16.9 MJ/kg regardless of its thickness while the peak heat release rate and maximum $CO_2$ concentration was varied with thickness.

Auto-detection of Halo CME Parameters as the Initial Condition of Solar Wind Propagation

  • Choi, Kyu-Cheol;Park, Mi-Young;Kim, Jae-Hun
    • Journal of Astronomy and Space Sciences
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    • v.34 no.4
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    • pp.315-330
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    • 2017
  • Halo coronal mass ejections (CMEs) originating from solar activities give rise to geomagnetic storms when they reach the Earth. Variations in the geomagnetic field during a geomagnetic storm can damage satellites, communication systems, electrical power grids, and power systems, and induce currents. Therefore, automated techniques for detecting and analyzing halo CMEs have been eliciting increasing attention for the monitoring and prediction of the space weather environment. In this study, we developed an algorithm to sense and detect halo CMEs using large angle and spectrometric coronagraph (LASCO) C3 coronagraph images from the solar and heliospheric observatory (SOHO) satellite. In addition, we developed an image processing technique to derive the morphological and dynamical characteristics of halo CMEs, namely, the source location, width, actual CME speed, and arrival time at a 21.5 solar radius. The proposed halo CME automatic analysis model was validated using a model of the past three halo CME events. As a result, a solar event that occurred at 03:38 UT on Mar. 23, 2014 was predicted to arrive at Earth at 23:00 UT on Mar. 25, whereas the actual arrival time was at 04:30 UT on Mar. 26, which is a difference of 5 hr and 30 min. In addition, a solar event that occurred at 12:55 UT on Apr. 18, 2014 was estimated to arrive at Earth at 16:00 UT on Apr. 20, which is 4 hr ahead of the actual arrival time of 20:00 UT on the same day. However, the estimation error was reduced significantly compared to the ENLIL model. As a further study, the model will be applied to many more events for validation and testing, and after such tests are completed, on-line service will be provided at the Korean Space Weather Center to detect halo CMEs and derive the model parameters.