• 제목/요약/키워드: mass of the suspension

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Effect of Light, Temperature, and Shaking Speed on Production of Capsaicin in Suspension-Cultured Jalapeno Pepper (Capsicum annuum L.)

  • Lee, Kwon-Bok;Engler, Cady;Yang, Jae E.;Lee, Shin-Woo;Park, Yong-Ha
    • Journal of Applied Biological Chemistry
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    • v.44 no.2
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    • pp.84-86
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    • 2001
  • Capsaicin synthesis by suspension cultured cells of Jalapeno pepper (Capcicum annuum L.) was assessed in vitro under various conditions including temperature (23 and $30^{\circ}C$), light intensity (with light and without light), and shaking speed (110 and 200 rpm). Capsaicin production increased, while the cell biomass growth decreased possibly due to the production of a secondary metabolite. Capsaicin synthesis was primarily affected by light condition. Cells cultivated at 110 rpm and $23^{\circ}C$ under light condition yielded the highest fresh weight, while those cultivated under the same condition, but without light resulted in the lowest cell mass. Capsaicin content in cells of 18-day-old pepper grown at 110 rpm and $23^{\circ}C$ under light was 0.125% of the cell mass. However, without light treatment, the capsaicin content in cells at the same shaking speed and temperature increased up to 169%, indicating no light is favored in the capsaicin synthesis by Jalapeno pepper. Increasing the shaking speed from 110 to 200 rpm without light enhanced the capsaicin synthesis. Results of this study demonstrate that light condition is the limiting factor in the synthesis of capsaicin in tissue-cultured Jalapeno pepper cells.

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Comparison of Muscle Atrophy Induced by Cast Fixation, Denervation and Suspension of Rat Hindlimb (흰쥐의 후지 석고 고정, 탈신경 및 부유에 의한 근위축의 비교)

  • Yoon, Bum-Chul;Lee, Myoung-Hwa;Kim, Nan-Soo;Hong, Hye-Jung;Yu, Byong-Kyu
    • The Journal of Korean Physical Therapy
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    • v.13 no.3
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    • pp.665-675
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    • 2001
  • The aim of this study was to compare features of muscle atrophy induced by cast fixation. denervation and suspension of rat hindimb. Muscle mass and glycogen of the soleus and plantaris muscles were studied after 3, 7, or 14 days of cast fixation, denervation and suspension. The results as follows: 1. Body weight of rats decreased significantly after 3 days and showed gradually increase after 7 and 14 days of hindlimb cast fixation, denervation and suspension. Particularly hindlimb suspended rats showed a rapid decrease after 3 days in body weight. 2. Relative weight of soleus and plantaris musclcs decreased significantly by hindlimb cast fixation, denervation and suspension, particularly after 7 days. The decrease rate was the lowest in suspended rats. 3. Glycogen content of soleus muscle decreased significantly after 14 days of hindlimb cast fixation, denervation and suspension. Also glycogen content of plantaris muscle decreased significantly after 14 days of hindlimb cast fixation and denervation, but not significantly after hindlimb suspension. These results indicate that suspension of hindlimb muscles causes less atropy than cast fixation or denervation, likely due to maintainment a few activities during hindlimb suspension. We concluded that the decrease in mechanical strains imposed on the muscle during inactivity was the main factor for the development of atrophy. These basic data suggest that some experimental conditions such as electrostimulation or stretching, participate in countermeasure programmes.

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Low Frequency Vibration of the Sprung Mass on Front Outer Wheel in Cornering (선회 시 차량의 외측전륜 스프링 상질량의 저주파 진동)

  • Lee, Byung-Rim;Lee, Jae-Eung
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2000.06a
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    • pp.1889-1893
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    • 2000
  • During the test drive of developing vehicle, a low frequency vibration of sprung mass on front outer wheel has been frequently observed in cornering with some speed. The purpose of this paper is to investigate the low frequency vibration of the sprung mass. The analysis result shows that the low frequency vibration is caused by sudden migration of the center of gravity of vihicle and it is determined by geometric points of suspension.

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Heat Transfer Characteristics of Liquid-Solid Suspension Flow in a Horizontal Pipe

  • Ku, Jae-Hyun;Cho, Hyun-Ho;Koo, Jeong-Hwan;Yoon, Suk-Goo;Lee, Jae-Keun
    • Journal of Mechanical Science and Technology
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    • v.14 no.10
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    • pp.1159-1167
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    • 2000
  • Particles in liquid-solid suspension flow might enhance or suppress the rate of heat transfer and turbulence depending on their size and concentration. The heat transfer characteristics of liquid-solid suspension in turbulent flow are not well understood due to the complexibility of interaction between solid particles and turbulence of the carrier fluid. In this study, the heat transfer coefficients of liquid-solid mixtures are investigated using a double pipe heat exchanger with suspension flows in the inner pipe. Experiments are carried out using spherical fly ash particles with mass median diameter ranging from 4 to $78{\mu}m$. The volume concentration of solids in the slurry ranged from 0 to 50% and Reynolds number ranged from 4,000 to 11,000. The heat transfer coefficient of liquid-solid suspension to water flow is found to increase with decreasing particle diameter. The heat transfer coefficient increases with particle volume concentration exhibiting the highest heat transfer enhancement at the 3% solid volume concentration and then gradually decreases. A correlation for heat transfer to liquid-solid flows in a horizontal pipe is presented.

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Performance Evaluation of a Semi-active Vehicle Suspension Using Piezostack Actuator Valve (압전작동기 밸브를 이용한 반능동 차량현가장치의 성능 고찰)

  • Han, Chulhee;Yoon, Gun-Ha;Park, Young-Dai;Choi, Seung-Bok
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.26 no.1
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    • pp.82-88
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    • 2016
  • This paper proposes a new type of semi-active direct-drive valve(DDV) car suspension system using piezoelectric actuator associated with displacement amplifier. As a first step, controllable piezoelectric DDV damper is designed and governing equation of a quarter-vehicle suspension system consisting of sprung mass, spring, tire and the piezostack DDV damper is constructed. After deriving the equations of the motion, in order to control spool displacement and damping force the skyhook controller is designed and applied. The performance evaluation of the proposed semi-active suspension system is conducted with different displacement of spool. Then, the ride comfort analysis is undertaken in time domain with bump road profile.

Control of Semi-active Suspensions for Passenger Cars(I) (승용차용 반능동 현가시스템의 제어)

  • Jo, Yeong-Wan;Lee, Gyeong-Su
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.21 no.12
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    • pp.2179-2186
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    • 1997
  • In this paper, the performance of a semi-active suspension system for a passenger car has been investigated. Alternative semi-active suspensions control laws has been compared via simulations. The control laws investigated in this study are : sprung mass velocity feedback control law, sky-hook damping control law, and state feedback control law. Simulation results show that a semi-active suspension has potential to improve ride quality of automobiles.

Analytical Study on the Gas-Solid Suspension Flows through Sonic and Supersonic Nozzles (음속 및 초음속 노즐을 통한 Gas-Solid Suspension 유동에 대한 해석적 연구)

  • Sun, JianGuo;Rajesh, G.;Kim, Heuydong
    • Journal of the Korean Society of Propulsion Engineers
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    • v.17 no.1
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    • pp.9-17
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    • 2013
  • A considerable deal of work has been carried out to get an insight into the gas-solid suspension flows and to specify the particle motion and its influence on the gas flow field. In this paper an attempt is made to develop an analytical model to study the effect of nozzle inlet/exit pressure ratio, particle/gas loading and the particle diameter effect on gas-solid suspension flow. The effect of the particle/gas loading on the mass flow, Mach number, thrust coefficient and static pressure variation through the nozzle is analyzed. The results obtained show that the presence of particles seems to reduce the strength of the shock wave. It is also found that smaller the particle diameter is, bigger will be the velocity as bigger particle will have larger slip velocity. The suspension flow of smaller diameter particles has almost same trend as that of single phase flow with ideal gas as working fluid. Depending on the ambient pressure, the thrust coefficient is found to be higher for larger particle/gas loading or back pressure ratio.

Vibration characteristics between levitation air-gap and switching system girders (상전도 흡인식 자기부상열차 분기기 주행시의 부상공극변동과 분기기 거더의 진동 특성)

  • Shin, Hyeon-Jae;Lee, Jong-Min;Choi, Jang-Young
    • Proceedings of the KSR Conference
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    • 2011.05a
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    • pp.824-829
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    • 2011
  • EMS-type Maglev vehicle maintains constant levitation air-gap between electromagnet and guideway by using gap sensor. The other words, when Maglev vehicles levitating over the guideway, mass of the vehicle effects through 1st (electromagnetic air-gap control) and 2nd (air-spring) suspension to grider. Resonace between electromagnetic suspension and grider could be occurred, which causes instability and poor ridecomfort. This paper is to test the dymanic interaction between levitation air-gap and switching system grider that has less mass and high natural frequencies than other type of general girders.

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Use of Dactylaria brochopaga, a Predacious Fungus, for Managing Root-Knot Disease of Wheat (Triticum aestivum) Caused by Meloidogyne graminicola

  • Kumar, Niranjan;Singh, K.P.
    • Mycobiology
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    • v.39 no.2
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    • pp.113-117
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    • 2011
  • A laboratory experiment was conducted to study the induction of constricting rings and test predation of Dactylaria brochopaga isolates against second stage juveniles (J2s) of Meloidogyne graminicola. Among the five fungal isolates, isolate D showed the greatest number of predatory rings and, consequently, trapped the maximum number of M. graminicola J2s in dual cultures. Another pot experiment was conducted to study the effect of D. brochopaga (isolate D) on the management of wheat root-knot disease. Applying a mass culture (10 g/pot) and a spore suspension of the fungus with and without cow dung manure to soil infested with 2,000 M. graminicola juveniles significantly improved plant height, root length, weights of shoots, roots, panicles and grains per hill compared to those in the control. Moreover, the fungus significantly reduced the number of root-knots, the number of egg masses, juveniles, and females per hill compared to those in the control. Bio-efficacy of the fungus was heightened when the mass culture and a spore suspensions were used in combination with cow dung manure to improve the plant growth parameters and reduce the number of root-knot and reproductive factors. Further investigations should be conducted to identify the impact of this fungus in the field.

Mass Propagation of Dicentra spectabilis L. Lemaire Through In vitro Suspension Culture (현탁배양을 통한 금낭화(Dicentra spectabilis L. Lemaire)의 대량증식)

  • Lee, Kang-Seop;Sim, Ock-Kyeong;Shin, Jeong-Sun;Choi, Yong-Eui;Kim, Ee-Yup
    • Journal of Plant Biotechnology
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    • v.31 no.2
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    • pp.121-126
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    • 2004
  • Bleeding heart (Dicentra spectabilis L. Lemaire) is one of the most valuable wild flower in Korea. This work was conducted for the mass production of somatic embryos through suspension culture and more effective plant regeneration system in Dicentra spectabilis. High-frequency embryogenic callus proliferation was achieved in SH liquid medium supplemented with 1 mg/L 2,4-D. Half-strength SH medium was suitable concentration for somatic embryo induction and germination. About 5,000 embryos were produced per 250$m\ell$ flask after 4 weeks of culture. Germination rate of somatic embryos was decreased when GA$_3$ was added in medium. The plantlets showed a 58% survival rate when transferred to pots after 1 month of culture. The results indicate that micropropagation procedure via somatic embryogenesis can be applied for an efficient mass propagation of Dicentra spectabilis.