• Journal of the Korean Society for Advanced Composite Structures
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• v.3 no.1
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• pp.21-28
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• 2012

In this paper, we present the analytical study results pertaining to the buckling of the orthotropic plates and local buckling of structural compression members composed of orthotropic plate components. Fiber reinforced polymeric plastic (FRP) materials, have many advantages over conventional structural materials such as steel and concrete. The advantages of the FRP materials are high specific strength and stiffness, high corrosion resistance, right weight, etc. Among the various manufacturing methods, pultrusion process is one of the best choices for the mass production of structural plastic members. Since the major reinforcing fibers are placed along the axial direction of the member, this material is usually considered as an orthotropic (tranversely isotropic, more specifically) material. However, pultruded fiber reinforced plastic structural members have low modulus of elasticity and are composed of orthotropic thin plate components the members are prone to buckle. Therefore, stability is an important issue in the design of the pultruded FRP structural members. In this paper, the buckling of orthotropic plates and the local buckling of pultruded FRP structural members are investigated by following the previous research results and the local buckling strength of the member produced in the domestic manufacturer is found.

• Journal of Environmental Health Sciences
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• v.36 no.2
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• pp.155-162
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• 2010

Aflatoxin $B_1$, a known human carcinogen, is the member of aflatoxin subfamily that is most frequently found in contaminated foods. Epidemiological studies have suggested that aflatoxins may be associated with human liver cancer and acute hepatitis. Recently it was reported that the traditional medical herbs sold in domestic markets are contaminated with aflatoxins. Long-term administration of these contaminated medicines could result in adverse health effects. Therefore, it is important to evaluate the levels of exposure to aflatoxin in people who ingest traditional herbal medicines. Blood samples were collected, before and after the herbal medicine intake, from 151 subjects who visited the hospital. The metabolite of aflatoxin $B_1$ in blood, aflatoxin $B_1$-albumin (aflatoxin $B_1$-lysine), is reportedly an appropriate internal exposure indicator, and its levels in the collected bloods were therefore analyzed using a liquid chromatography-mass spectrometry. The analytical method of aflatoxin $B_1$-lysine in blood was firstly optimized in Korea and the levels were detected below quantification limits (2 pg/mg albumin) in this study population. Consequently, the exposure levels of aflatoxin $B_1$ by ingestion of herbal medicines were low but it is important to monitor routinely due to the possibility of risk on the aflatoxin exposure.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.4 no.4
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• pp.305-311
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• 1999

This study aims to decipher surface water mass interaction in summer in the South China Sea and East China Sea by radium isotope distribution pattern. Salinity and activity ratio of radium ($^{228}Ra/^{226}Ra$) showed gradual changes, which were adequate to apply simple two end-member mixing between Kuroshio surface water and Changjiang Dilute Water for the East China Sea and the former and Nearshore Diluted Watermass (NDW) for the South China Sea. Two tracer methods, salinity and Ra isotope ratio, were compared for East China Sea. Results showed remarkable consistency for waters near Kuroshio, however, discrepancy were noticeable after Tsushima Warm Current branching. Mixing with subsurface waters may cause the discrepancy. When mixed with subsurface waters, salts and radium isotope ratio are expected to be biased in opposite direction, i. e. prone to underestimate the fraction of less saline water in the case of salts and vice versa for Ra isotope ratio. Taking the mean values of two different results seems more realistic to estimate fraction of end-members.

• Journal of the Korean Society for Advanced Composite Structures
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• v.5 no.2
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• pp.1-8
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• 2014

Fiber reinforced polymeric plastic (FRP) materials have many advantages over conventional structural materials, i.e., high specific strength and stiffness, high corrosion resistance, right weight, etc. Among the various manufacturing methods, pultrusion process is one of the best choices for the mass production of structural plastic members. Since the major reinforcing fibers are placed along the axial direction of the member, this material is usually considered as an orthotropic material. However, pultruded FRP (PFRP) structural members have low modulus of elasticity and are composed of orthotropic thin plate components the members are prone to buckle. Therefore, stability is an important issue in the design of the pultruded FRP structural members. Many researchers have conducted related studies to publish the design method of FRP structures and recently, referred to the previous researches, pre-standard for LRFD of pultruded FRP structures is presented. In this paper, the accuracy and suitability of design equation for the local buckling strength of pultruded FRP I-shape compression members presented by ASCE are estimated. In the estimation, we compared the results obtained by design equation, closed-form solution, and experiments conducted by previous researches.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.3
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• pp.439-452
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• 1994

A method to optimize the cost of R/C frames and an algorithm of the optimal limit state design for R/C frames subjected to dynamic loads are presented. The modal superposition method was used to find the dynamic responses of the frames. Each member of R/C frame is made up of more than two elements and the stiffness matrix and consistent mass matrix of three d.o.f in the node of each element was used to include axial, shear and flexural effects. The objective function to be minimized formulated the cost of materials, steel and concrete, and optimised to satisfy the behaviors of R/C frame and each constraint imposed by the limit state requirements. Both objective function and each constraint are derived in terms of design variables which include the effective depth, beam width, compression and tension steel area, and column shear steel area. A few applications are presented which demonstrate the feasibility, the validity and efficiency of the algorithm for automated optimum design of R/C frames where dynamic behavior is to be considered.

• Reproductive and Developmental Biology
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• v.31 no.4
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• pp.253-260
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• 2007

To examine the differential protein expression pattern in the 11.5 day post-coitus (dpc) and 18.5 dpc placenta of mouse, we have used the global proteomics approach by 2-D gel electrophoresis (2-DE) and MALDI-TOF-MS. The differential protein patterns of 3 placentae at the 11.5 dpc and 18.5 dpc from nature mating mice were analyzed. Proteins within isoelectric point range of $3.0{\sim}10.0$, separately were analyzed in 2DE with 3 replications of each sample. A total of approximately 1,600 spots were detected in placental 2-D gel stained with Coomassie-blue. In the comparison of 11.5 dpc and 18.5 dpc placentae, a total of 108 spots were identified as differentially expressed proteins, of which 51 spots were up-regulated proteins such as alpha-fetoprotein, mKIAA0635 protein and transferrin, annexin A5, while 48 spots were down-regulated proteins such as Pre-B-cell colony-enhancing factor l(PBEF), aldolase 1, A isoform, while 4 spots were 11.5 dpc specific proteins such as chaperonin and Acidic ribosomal phosphoprotein P0, while 3 spots were 18.5 dpc specific proteins such as aldo-keto reductase family 1, member B7 and CAST1/ERC2 splicing variant-1. Most identified proteins in this analysis appeared to be related with catabolism, cell growth, metabolism and regulation. Our results revealed composite profiles of key proteins involved in mouse placenta during pregnancy.

• Structural Monitoring and Maintenance
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• v.4 no.2
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• pp.153-173
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• 2017

An improved method is presented to estimate the axial force of a bar member with vibrational measurements based on modified Timoshenko beam theory. Bending stiffness effects, rotational inertia, shear deformation, rotational inertia caused by shear deformation are all taken into account. Axial forces are estimated with certain natural frequency and corresponding mode shape, which are acquired from dynamic tests with five accelerometers. In the paper, modified Timoshenko beam theory is first presented with the inclusion of axial force and rotational inertia effects. Consistent mass and stiffness matrices for the modified Timoshenko beam theory are derived and then used in finite element simulations to investigate force identification accuracy under different boundary conditions and the influence of critical axial force ratio. The deformation coefficient which accounts for rotational inertia effects of the shearing deformation is discussed, and the relationship between the changing wave speed and the frequency is comprehensively examined to improve accuracy of the deformation coefficient. Finally, dynamic tests are conducted in our laboratory to identify progressive axial forces of a steel plate and a truss structure respectively. And the axial forces identified by the proposed method are in good agreement with the forces measured by FBG sensors and strain gauges. A significant advantage of this axial force identification method is that no assumption on boundary conditions is needed and excellent force identification accuracy can be achieved.

• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.1011-1017
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• 2001

Streptomyces sp. Y-110 cytochrome P-450, induced by the addition of compactin -Na into the culture medium, was purified from the cell extract to apparent homogeniety, mainly by DEAE-Sepharose, hydroxyapatite, and Mono Q column chromatyography. The sepcific activity of purified enzyme on its substrate, compactin-Na, was determined to be 15 nmol of pravastatin per mg protein. The molecular mass of this enzyme on SDS-PAGE was $37{\pm}0.5$ kDa, pI was 4.5, and its CO difference spectrum showed maximum absorption peaks at 452 and 550nm, respectively. The N-terminal amino acid sequence was determined to be Met>Thr>Cys>Thr>Pro>Val>Thr>Val>The>Gly>Ala>Ala>Gly>Gln>Ile>Gly>Tyr>Ala>Leu. Its apparent $K_m$ on compactin-Na was $1.294{\mu}M{\cdot}min^-1,\;and\;V_{max}\;was\;1.028{\mu}M{\cdot}min^-1$. The maximum substrate concentration ($K_s$) for reaction was $270 {\mu}M$and thus $1/[K_s]$ was $3.7{\mu}M$. These physicochemical characteristics and kinetic behavior of this enzyme were compared and shown to be different from those of Streptomyces cytochrome P-450 enzymes reported, suggesting that this enzyme may be an additional member of the Streptomyces cytochrome P-450 family.

• Journal of Microbiology and Biotechnology
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• v.24 no.9
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• pp.1260-1268
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• 2014

Screening of a gene library from Paenibacillus sp. PBS-2 generated in Escherichia coli led to the identification of a clone with lipolytic activity. Sequence analysis showed an open reading frame encoding a polypeptide of 378 amino acid residues with a predicted molecular mass of 42 kDa. The esterase displayed 69% and 42% identity with the putative ${\beta}$-lactamases from Paenibacillus sp. JDR-2 and Clostridium sp. BNL1100, respectively. The esterase contained a Ser-x-x-Lys motif that is conserved among all ${\beta}$-lactamases found to date. The protein PBS-2 was produced in both soluble and insoluble forms when E. coli cells harboring the gene were cultured at $18^{\circ}C$. The enzyme is a serine protein and was active against p-nitrophenyl esters of $C_2$, $C_4$, $C_8$, and $C_{10}$. The optimum pH and temperature for enzyme activity were pH 9.0 and $30^{\circ}C$, respectively. Relative activity of 55% remained at up to $5^{\circ}C$ with an activation energy of 5.84 kcal/mol, which indicates that the enzyme is cold-adapted. Enzyme activity was inhibited by $Cd^{2+}$, $Cu^{2+}$, and $Hg^{2+}$ ions. As expected for a serine esterase, activity was inhibited by phenylmethylsulfonyl fluoride. The enzyme was remarkably active and stable in the presence of commercial detergents and organic solvents. This cold-adapted esterase has potential as a biocatalyst and detergent additive for use at low temperatures.

• Journal of the Korean association of regional geographers
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• v.15 no.4
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• pp.464-477
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• 2009

This research analyzed reality of multicultural education of Korea and suggested policy implication through a questionnaire survey of teachers, students, and parents in elementary schools, Daegu City. Multicultural policies in Korea mainly helped a member of multicultural family who immigrated into Korea to learn Korean language and culture. Thus, the common people have a few chances to have multicultural education; however they mainly experienced multiculture indirectly through mass media. Therefore they had low level of understanding and satisfaction and their consciousness was narrow. Most of people completely come out in sympathy with that multicultural education should be taught at school. To overcome the current issues of multicultural education, its goals, contents and tasks should be clearly established. Multicultural education is taught from elementary school in holistic approach; however, methods of learning and teaching are different according to a curriculum.