• Herbal Formula Science
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• v.25 no.4
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• pp.499-508
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• 2017

Objectives : Skeletal muscle atrophy occurs in response to a variety of conditions. The unloading to muscle occurs clinically in limb immobilization, bed rest, spinal cord injury and peripheral nerve damage, resulting in significant loss of muscle mass and force production. Muscle disuse is accompanied by an increase in apoptotic signaling, which mediates some of the responses to unloading in the muscle. In this study we tested the hypothesis that Daeyeoung-jeon extract would improve muscle recovery after reloading following disuse. Method : Twenty young male Sprague-Dawley rats were used for the studies. The hindlimb immobilization was performed with casting tape to keep the left ankle joint in a fully extended position. No intervention was performed on the right leg and used as intact region. The Rats in Daeyeoung-jeon treated group (DYJ) were orally administrated Daeyeoung-jeon water extract, and rats of Control group were given with saline only. After 2 weeks of immobilization, all animals were sacrificed, and the whole gastrocnemius muscles were dissected from both legs. The morphology of right and left gastrocnemius muscles in both DYJ and Control groups were assessed by hematoxylin and eosin staining. Moreover, to investigate the immobilization-induced muscular apoptosis, the immunohistochemical analysis of Bax and Bcl-2 was carried out. Results : Daeyeoung-jeon represented the significant protective effects against the reductions of the left gastrocnemius muscles weight and average cross section area to compared with Control group. The treatment with Daeyeoung-jeon extract significantly reduced the immunoreactivity of BAX and increased the immunoreactivity of Bcl-2 in gastrocnemius muscle compared with Control group. Conclusion : Daeyeoung-jeon has protective effects against immobilization-induced muscle atrophy by regulating the activities of apoptosis-associated BAX/Bcl-2 proteins in gastrocnemius muscle.

• Herbal Formula Science
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• v.17 no.1
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• pp.29-43
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• 2009

This report carried out to study 59 prescriptions related to the use of Pueraria thunbergiana bentham in Dongueibogam This study is based on Dongueibogam which has been widely used for herbal clinic in Korea. And this study is investigated to make sure of the range of treatment, nature of disease, the pathology and the dosage of Pueraria thunbergiana bentham in Dongueibogam. The following conclusions were reached through investigations on the prescriptions that used in Dongueibogam as a various role. 1. The prescriptions that Pueraria thunbergiana bentham was taken as a 15 for monarchy, 15 for attendant, 11 for assistance and valet herb are utilized for therapeutic treatments such as cough, fever etc, mainly Sanghan syndromes in the chapter of Wind. 2. The Pueraria thunbergiana bentham was also utilized as a key ingradients in the chapter of Eye and Onyeok epidemic. 3. The most frequent mass of the Pueraria thunbergiana bentham when used as a monarchy herb is 2jeon, and the standard limited range of adding Pueraria thunbergiana bentham to medical prescription is 7pun$\sim$5jeon per dose. But, when used as a attendant herb, an typical and regular adding dose is the range of 3pun5ri$\sim$1yang2.5jeon,and 3pun$\sim$1.5jun in the case of assistant. 4. The phathogenic factors and phathology of Pueraria thunbergiana bentham in the prescription are based on the chapter of Cold when used as a monarchy herb and on the chapter of Wind in the case of attendant herb. 5. The Seungmagalgeontang is the most useful base prescription which use the Pueraria thunbergiana bentham as the main ingredient.

• The Journal of the Korea Contents Association
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• v.15 no.12
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• pp.141-152
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• 2015

If we look into genre movie, we can see that almost similar forms are repeated in a movie. Such similar elements are largely divided into three units from a mass of story to a very small camera angle. Those can be explained as Formula, Convention, and Iconography. Among those three, convention means custom and it is a structure or an incident in which one story can be divided into second one. Convention is an incident visualized in individual genre, and a movie director tunes audiences through the incident. The director leads a familiar story but all of a sudden, he transforms the familiar scene to a new story. As a product established from the beginning of movie history, movie convention helps communication between audiences and a director. Audiences familiarize themselves with movie convention through repeated activities of watching movies, and the director utilizes it to provide audiences with familiarity. Director Bong Joon-ho not only tunes audiences through traditional convention but also creates a new art work through transformation of convention. A study is conducted on how he used traditional convention and transformation to get a new idea and to engage in his work through his work .

• Herbal Formula Science
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• v.24 no.3
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• pp.153-161
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• 2016

Chronic alcoholic myopathy is one of the most common skeletal muscle disorders. It is characterized by a reduction in the entire skeletal musculature, skeletal muscle weakness, and difficulties in gait. Patients with alcoholic hepatitis and cirrhosis have severe muscle loss that contributes to worsening outcome. Although the myopathy selectively affects Type II (fast twitch, glycolytic, anaerobic) skeletal muscle fibers, total skeletal musculature is reduced. The severity of the muscle atrophy is proportional to the duration and amount of alcohol consumed and leads to decreased muscle strength. The mechanisms for the myopathy are generally unknown but it is not due to overt nutritional deficiency, nor due to either neuropathy or severe liver disease. Skeletal muscle mass and protein content are maintained by a balance between protein synthesis and breakdown and in vivo animal models studies have shown that ethanol inhibits skeletal muscle protein synthesis. Daekumeumja is a traditional Korean medicine that is widely employed to treat various alcohol-induced diseases. Muscle diseases are often related to liver diseases and conditions. The main objective of this study was to assess that Daekumeumja extract could have protective effect against alcoholic myopathy in a Sprague-Dawley rat model. Rats were orally given 25% ethanol (5ml/kg, body weight) for 8 weeks. After 30 minutes, rats were administrated with Daekumeumja extract. Controls were similarly administrated with the vehicle alone. The weights of gastrocnemius, soleus and plantaris muscles were assessed and the morphologic changes of gastrocnemius and plantaris muscles were also assessed by hematoxylin and eosin staining. In results, The muscles from ethanol treated rats displayed a significant reduction in muscle weight and average cross section area compared to Normal group. Daekumeumja extract treated group showed increased muscle weight and muscle fiber compared to the ethanol treated group. It was concluded that Daekumeumja extract showed ameliorating effects on chronic alcohol myopathy in skeletal muscle.

• Korean Journal of Community Nutrition
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• v.16 no.1
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• pp.37-50
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• 2011

Nutrients intake status of 73 lactating women, that is 45 breast feeding (BF), 13 formula feeding (FF) and 15 mixed feeding (MF), living in Daejeon was investigated. Self-recorded food intakes for two weekdays and eating behaviors using questionnaires were surveyed from May to August 2008. Subjects aged $29.2{\pm}3.4$ years and their infants aged $8.2{\pm}3.2$ months. Body mass index of the subjects was $21.0{\pm}3.2$. Of the subjects 23.3% were employed. Daily energy intake was $1953{\pm}391$ kcal. Mean adequacy ratio (MAR) was $0.77{\pm}0.14$ and was higher in FF ($0.86{\pm}0.13$) than in BF ($0.76{\pm}0.11$) and MF ($0.72{\pm}0.18$). Nutrients that over 50% of the subjects took less than estimated average requirement were vitamin A, riboflavin, folate, vitamin C and calcium. And index of nutritional qualities of those 5 nutrients were below one Forty six and sixths percent (46.6%) of the subjects showed GMFVD = 11111 pattern of five food group intakes and 57.5% took meals three times daily, 56.2% skipped often breakfast, and 64.4% dined out two times and more per week. Subjects having lower MAR (< 0.72, n = 24), compared with those having higher MAR (${\geq}$ 0.83, n = 24), showed more skipping daily meals and less frequency of eating-out, and took less legumes, vegetables, fishes, and milk. As the results, intakes of calcium, vitamin A, riboflavin, folate, and vitamin C were insufficient in lactating women, especially in BF and MF mothers. Meal skipping and low intakes of dairy foods, legumes, vegetables, and fishes might have adverse influences on nutritional status of lactating women. Accordingly, nutrition care program for lactating women should be focused on intake of three meals daily and a variety of food.

• Membrane and Water Treatment
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• v.10 no.6
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• pp.471-484
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• 2019

The complex combination of organic contaminants in the wastewater made water treatment challenging; hence, organic matter in water bodies is usually measured in terms of organic carbon. Since it is important to identify the types of compounds when deciding suitable treatment methods, this study implemented a quantitative and qualitative analysis of the organic matter content in an actual graywater sample from Ulsan, Republic of Korea using mass spectroscopy (MS). The graywater was treated using adsorption to remove the organic contaminants. Using orbitrap MS, the organic matter content between an untreated graywater and the treated effluent were compared which yielded a significant formula count difference for the samples. It was revealed that CHON formula has the highest removal count. Isotherm studies found that the Freundlich equation was the best fit with a coefficient of determination ($R^2$) of 0.9705 indicating a heterogenous GAC surface with a multilayer characteristic. Kinetics experiments fit the pseudo-second order equation with an $R^2$ of 0.9998 implying that chemisorption is the rate-determining step between the organic compounds and GAC at rate constant of $52.53g/mg{\cdot}h$. At low temperatures, the reaction between GAC and organic compounds were found to be spontaneous and exothermic. The conditions for optimization were set to achieve a maximum DOC and TN removal which yielded removal percentages of 94.59% and 80.75% for the DOC and TN, respectively. The optimum parameter values are the following: pH 6.3, 2.46 g of GAC for every 30 mL of graywater sample, 23.39 hrs contact time and $38.6^{\circ}C$.

• Journal of the Korean GEO-environmental Society
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• v.12 no.1
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• pp.5-12
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• 2011

In this study, correlation between measured deformation modulus using pressuremeter and RMR value conducted in 10 sites is analyzed, and applicability of the conventional empirical formulas to the rock masses in Korea are analyzed, It is found that if RMR is below 40, the correlation between deformation modulus and RMR accords Kim Gyo-won's formula and Aydan, Serafim and Pereira's one well, but if the RMR exceeds 40, the correlation was lower than those from the formula. Such results may be attribute to the fact that during classification of RMR, scores are weighed relatively more in joint conditions and apertures than such highly correlational items as uniaxial compression strength or RQD, and RMR would not be evaluated qualitatively due to different weathering degrees and rock mass types as well as engineers' personal errors. Sandstone among sedimentary rocks are quite well accord with suggested equation, but correlation of other rocks are due to large variance. In this study, correlation expressions of various rocks are proposed as the function of exponential based on the field test data.

• Herbal Formula Science
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• v.16 no.1
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• pp.15-27
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• 2008

This report describes 46 studies related to the use of Fructus Ponciri Seu Aurantii main blended prescriptions from Dongeuibogam. The following conclusions were reached through investigations on the prescriptions that use Fructus Ponciri Seu Aurantii as a key ingredient. 1. 19.6% of feces recorded the largest number of clinical frequency of the prescriptions in therapeutic use when Fructus Ponciri Seu Aurantii was taken as a monarch drug in prescriptions. In addition, 13.0% of each of a cough and an abdominal mass with distention and pain ranked second. 2. Prescriptions that utilize Fructus Ponciri Seu Aurantii as the main ingredient are used in the treatmeant of 5 diseases related to each of feces and an abdominal mass with distention and pain, and they are also used for treating different types of diseases related to the following ; a cough, a chest, ribs, eyes, the fullness in the chest, Qi, skin areas. 3. In the view of the causative agent of a disease, the prescriptions which are compounded with Fructus Ponciri Seu Aurantii as a monarch drug are related to endogenous agents such as seven emotion, food, deficiency, exogenous agents such as wind-cold pathogen, heat and non-endo-exopathogcnic factors like diseases due to external factors, poison. And in the view of the pathology of a disease, they are applied to the viscera pathology related to the lung, the spleen and stomach, the pathology of Qi and blood related to the reversed flow of Qi, the congestion of Qi, the deficiency of blood, the obstruction of Qi and blood, and the pathology about the retention of phlegm and fluid related to phlegm stagnation. 4. The dosage of Fructus Ponciri Seu Aurantii is 1.25pun(about 0,47g) to 2jeon(about 7.5g), however 1jeon(about 3.75g) has been taken the most for clinical application. 5. We can find out that according to herbs or prescriptions blended with itself, Fructus Ponciri Seu Aurantii makes a variety of functions to penetrate and remove stagnation, regulate Qi flow, relieve stagnation, expell wind and get rid of pain.

• Herbal Formula Science
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• v.24 no.2
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• pp.80-99
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• 2016

Objectives : Instrumental chemical analysis was utilized to investigate the effect of Add-Omit-Saenghyeoryunbu-eum(AO-SHU) on diabetic treatment. One of the most exciting, yet also controversial, arguments is the safety and biological mechanisms of the natural medicine on human body. Therefore, the aim of this study is to provide a better understanding on bioactive chemical components, hazards of heavy metal contamination and biological mechanism of the diabetic medicine composed of 12 different natural herbs. Methods : To study bioactive compound and metallic component in the diabetic medicine in detail, LC-MS/MS (Liquid Chromatography-Mass/Mass), GC (Gas Chromatography) and ICP (Inductively Coupled Plasma) were utilized to characterize the extract of the diabetic medicine and the result was compared with 18 marker substances selected from literature survey. In addition, in vitro assay experiments including GPR 119 activity and human DGAT-1 inhibition, and OGTT (Oral Glucose Tolerance Test) were performed to verify the effectiveness of this medicine on diabetic treatment. Results : Out of 18 marker substances, 9 bioactive compounds were identified from LC-MS/MS analysis which include Citruline, Catalpol, Berberine, Ginsenoside Rb1, Ginsenoside Rg1, Oleanolic acid, β-Sitosterol, Mangiferin, and Schizandrin. ICP study on 245 residual pesticides revealed that 239 species were not detected but 6 species, Dimethomorph, Trifloxystrobin, Pyraclostrobin, Isoprocarb, Carbaryl and Flubendiamide, while the amounts are trace levels, below permitted concentrations. The biological activity was observed in vitro assay and Oral Glucose Tolerance Test(OGTT), which are consistent with a preliminary clinical test result, a drop in blood sugar level after taking this herbal medicine. Conclusions : Instrumental chemical analysis using LC-MS/MS, GC, and ICP was conducted successfully to identify bioactive compounds in AO-SHU for the treatment of diabetes, finding 9 bioactive compounds. Furthermore, in vitro assay experiments and OGTT show that AO-SHU has its biological activities, which imply that it can be a candidate for the future diabetes remedy.

• Herbal Formula Science
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• v.19 no.2
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• pp.161-178
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• 2011

Objectives : The aim of this study is to find the therapeutic meaning of Rhizoma Cyperi in herbal medication. Methodes : In this study, we investigate 85 prescriptions related to the use of Rhizoma Cyperi main blended from Dongeuibogam. we research about field of therapy, symtoms of a disease, the chief virtue of a medicine, the cause of a disease, the pathology of a disease, dosage, contents of composition. Results : The following conclusions were reached through investigations on the prescriptions that use Rhizoma Cyperi as the main ingredient. Prescriptions that Rhizoma Cyperi was taken as a monarch drug are utilized for 24 therapeutic purpose, for example, woman disease, uterine disease, vitality and abdominal mass. In particular, 16.4% of prescriptions appear in the chapter of woman, and 15.2% of those appear in the chapter of uterus. Prescriptions that utilize Rhizoma Cyperi as the main ingredient are used in the treatment of menstrual disorder, pain due to disorder of Gi, infertility, irregular menstruation and abdominal mass. Rhizoma Cyperi is used in pathogenic factors such as seven modes of emotions and used in pathology related to the stagnation of blood system. The dosage of Rhizoma Cyperi is 6li(about 0.23g) to 6don(about 22.5g), however 1don(about 3.75g) has been taken the most for clinical application. Gyogamdan and Yukmisamleunghwan are the most useful base prescriptions which use the Rhizoma Cyperi as the main component. Conclusions : This information provide the rationale for establishing Basic evidance in the oriental medical prescription.