• Fisheries and Aquatic Sciences
• /
• 제8권4호
• /
• pp.207-212
• /
• 2005

Directional (sinistral) asymmetry (DA) occurs when the traits of one side of a supposedly bilaterally symmetrical organism differ in a random way from those of the other side. We examined asymmetries in gonadal growth traits within both sexes of hatchery reared ayu, Plecoglossus altivelis. The pattern of the gonadal growth from hatching to 320 days after hatching was a type of DA except for the growth in gonad weight from 140 to 180 days after hatching, although the ovary of the right side tended to exhibit more pronounced DA phenomenon.

• 한국환경과학회지
• /
• 제25권10호
• /
• pp.1397-1405
• /
• 2016

Mussels are stubborn organism attached to solid substrate by byssus threads and caused operational problems in utility of power generating stations. Sole and combined usage of ultrasonic (28 kHz- and 42 kHz- frequencies) and hydrogen peroxide ($H_2O_2$) has studied for control of blue mussel larvae and adult stage in seawater condition. A theoretical wo rking model using disinfection (Chick and Watson type) approaches is presented based on helpful results of experiments. This study also demonstrate that the combined treatment (ultra-sonication with $H_2O_2$) is overall highly efficient than individual treatment would, but on the basis of exposure time, the ultra-sonication was the most efficient among them. Therefore the development of sole and combined technique might be effective practical mitigation strategy against mussel attachment for water handling facilities.

• 해양환경안전학회:학술대회논문집
• /
• 해양환경안전학회 2017년도 추계학술발표회
• /
• pp.209-209
• /
• 2017

• Ocean and Polar Research
• /
• 제29권3호
• /
• pp.273-282
• /
• 2007

For an efficient management and utilization of marine biodiversity information, we made an attempt to develop the Korea Marine Biodiversity Information System (KoMBIS), building a species name inventory of Korea marine organisms. The inventory includes 17 organism groups: phytoplankton, zooplankton, algae and halophyte, sponges, cnidarians, rotifers, nematodes, bryozoans, brachiopods, molluscs, echiurans, annelids, arthropods, echinoderms, urochordates and fish. The species names were collected from 37 different references and reviewed for validity by taxonomists, which resulted in 9,798 valid names in addition to 1,845 synonyms. The Korea marine species inventory is the first one of this kind, for previous Korean species name inventories were mostly composed of terrestrial and freshwater organisms. KoMBIS, the information system developed, contains not only the species name but also information on morphological and ecological characteristics such as distribution, DNA barcode, and references. This system is convenient for the inputting of new data and servicing users through the internet, so that management and utilization of the biodiversity information is more efficient. Linking the DNA barcode data with species information provides an objective measure for identification of a species, which accommodates the recommendation of Consortium for the Barcode of Life, and makes the Korea marine biodiversity information compatible with international databases. Considering the frequent exchange of marine organisms internationally via ballast water and such issues as climate change, this information system will be useful in many areas of marine biodiversity.

• 한국수산과학회지
• /
• 제34권6호
• /
• pp.697-704
• /
• 2001

단순 구성된 동물 먹이생물 급이 계열 (로티퍼-알테미아)을 이용하는 해산어의 종묘 생산 과정에서는 알테미아 수급 불균형 및 가격 파동 발생으로 알테미아를 대체할 수 있는 새로운 먹이생물의 개발을 요구하고 있다. 이 연구에서는 알테미아 대체 먹이생물로서 담수산 물벼룩 M. macrocopa과 기수산 물벼룩 D. celebensis의 이용 가능성을 검토하기 위한 일환으로 해수에서의 반응 결과 (생존과 증식)를 알아보았다. 담수산 물벼룩 M. macrocopa과 기수산 물벼룩 D. celebensis을 이용하여 배양 환경으로서 다양한 염분 조건으로의 점프 이동 후 두 종 물벼룩의 생존과 증식 반응을 관찰하기 위하여 16일간의 실험 기간 중 매일의 증식 상태와 단시간 생존율을 관찰하였다. 이 연구 결과, 기수산 물벼룩 D. celebensis는 0 ppt의 염분 배양 환경을 제외한 전 실험구에서 비교적 양호한 생존과 증식 반응이 관찰된 반면, 담수산 물벼룩 M. macrocopa은 염분 농도 4 ppt 이상의 점프 이동 환경하에서는 섭이 행동과 유영 행동이 급속히 둔화되면서 증식은 물론 생존조차도 불가능한 것을 알 수 있었다. 특히, 담수산 물벼룩 M. macrocopa의 단시간 생존율을 관찰한 결과에서는 3 ppt 이상의 염분 농도로 점프 이동한 경우에는 점프이동 후 5분 이내에 일부 사망 개체가 발생하는 것이 관찰되었다. 이 연구의 결과, 기수산 물벼룩 D. celebensis은 담수산 물벼룩 M. macrocopa과는 달리 현재 많은 연구가 수행되고 있는 코페포 다와 함께 해산어의 종묘 생산 과정에서 알테미아의 대체 먹이생물로 연구를 진행할 가치가 있는 것으로 판단되었다.

• Environmental Analysis Health and Toxicology
• /
• 제25권1호
• /
• pp.19-26
• /
• 2010

Discharged materials from the point or non-point source are released into the sea, and as the results, marine environment is directly affected. We must estimate the impacts of contaminants to marine pollution rapidly and accurately. Therefore, it is needed on early warning system for appreciating marine environmental impacts, and required a bioassay to evaluate abnormal changes. A bioassay test was developed to examine the effects of heavy metal contaminants on the early life stages of the marine annimals. We have studied the effects of metals on early development of a sea urchin species, Strongylocentrotus intermedius. S. intermedius embryos were tested with six metals (Cu, Ag, Zn, Cd, Cr, Ni) and showed the highest sensitivity to Cu as well as the lowest sensitivity to Cd. The order of biological impact for metals was Cu>Ag>Ni>Zn>Cr>Cd. In accordance with the results, sea urchins embryos can provide biological criteria for seawater quality assessment. The sensitivity of developmental bioassay whith S. intermedius is at intermediate level among marine organisms commonly used in aquatic bioassays. And this sea urchin can be routinely employed as a test organism for ecotoxicity assays.

• Journal of Microbiology
• /
• 제45권3호
• /
• pp.241-245
• /
• 2007

This study was conducted to determine the optimal conditions for introduction of exogenous DNA into Synechocystis sp. PCC 6803. Of the three transformation techniques studied, electroporation, ultrasonic transformation and natural transformation, natural transformation showed the highest efficiency. Additionally, this study demonstrated that the higher plasmid concentration and longer homologous recombining fragments resulted in a greater number of transformants. For successful transformation, the lowest concentration of plasmid was $0.02\;{\mu}g/ml$, and the shortest homologous recombining fragment was 0.2 kb. Use of Synechocystis sp. PCC 6803 in the logarithmic growth phase resulted in two-fold higher transformation rate than that of the same organism when cells in the latent phase or the plateau phase were used for transformation. Pretreatment of the host strain, Synechocystis sp. PCC 6803, with EDTA (2 mM) for two days prior to transformation increased the transformation efficiency by 23%. Additionally, incubation of the cells and DNA for 5 h under light conditions increased the transformation efficiency by two orders of magnitude. Moreover, recovery treatment of the cells before they were plated onto antibiotic medium also increased the transformation efficiency.

• 한국표면공학회지
• /
• 제55권6호
• /
• pp.425-432
• /
• 2022

Antifouling paints that inhibit the attachment and contamination of marine organisms mainly use TBT compounds, but because of their toxic components, they cause ecosystem disturbance and environmental destruction problems, so It is necessary to research eco-friendly antifouling paints that are easy to maintain and effective antifouling technologies. In this study, physical surface treatment of silane coating and chemical antifouling technology were applied to the metal surface to secure the stability of the surface of the marine structure and inhibit the attachment and growth of marine organisms. Adhesion of marine organisms was evaluated according to the coating conditions through surface evaluation of the charged material for 15 months in the waters of the west coast of Korea. In accordance with ASTM D6990-05, antifouling properties fouling rates (FR) and physical degradation rates(PDR) were evaluated through visual inspection of the evaluation specimens. As a result of evaluating the antifouling performance of the coated surface, it was confirmed that the antifouling performance was maintained at the 50% level even after 15 months in the sample subjected to physical processing and silane coating.

• 한국양식학회지
• /
• 제13권1호
• /
• pp.63-67
• /
• 2000

연안역에서 쉽게 관찰되는 해산 코페포다 Tigriopus Japonicus는 해산어의 종묘 생산 과정에서 동물 먹이 생물로서 이용되는 플랑크톤의 한 종이다. 그러나, 아직 T. japonicus의 대량 배양 및 안정 배양에 관한 기술은 미확립된 상태이며, 로티퍼와 함께 혼합 배양하는 방법이 비교적 다량의 T. japonicus의 수확이 가능한 것으로 알려져 있을 뿐이다. 이 연구에서는 로티퍼와이 혼합 배양조내에서 T. japonicus가 어떤 먹이원을 이용하여 성공적으로 증식 가능하였는지를 검토하였다. T. japonicus의 먹이로 급이한 Nannochloropsis oculata는 이용하지 않고 로티퍼 배양조의 부산물을 적극적으로 이용하지 않고. 로티퍼 배양조의 부산물을 적극적으로 이용하였다. 로티퍼 배양조의 부산물 먹이로 급이한 조건하에서 로티퍼는 그 증식이 크게 억제된 반면, 부산물만을 먹이로 급이한 T. japonicus의 어린 노플리우스 I 단계의 개체는 물론 코페포디드 I 단계의 개체도 정상적인 증식이 가능하였다. 난낭으로부터 갓 부화된 20개체의 어린노플리우스는 38일간의 로티퍼 배양조 부산물만의 단독 급이 조건하에서 배양 개시 후 30일째에 226.3${\pm}$24.1개체/40ml의 초고 밀도가 관찰된 것을 전후로 높은 밀도의 개체 유지가 가능하였다. 특히, 각 발생 단계별로 계수한 결과에서는 코페포디드 I단계에 로티퍼 배양조의 부산물만을 먹이로서 첨가한 결과에서도 유사한 결과가 관찰되었다. 결국 T. japonicus의 배양 밀도를 높이기 위해서는 노플리우스의 정상적인 성장을 도모하여 주어야 하며, 특히 노플리우스가 먹을 수 있는 상태의 먹이인 로티퍼 배양조의 부산물과 같은 먹이원이 코페포다의 배양 과정에 첨가되어야 할 것이다.

• 환경영향평가
• /
• 제21권1호
• /
• pp.1-13
• /
• 2012

We diagnosed on status and problems of environmental assessment regarding development of offshore wind power, and also on reasonable core assessment items. Most of the coastal wind power are located on the western coastline of Korea and Jeju Island. In the selections of the site for the offshore wind farms, a previous investigations should be conducted with regard to distances from the land, stabilities from external forces (tide, wave, etc.) and topographical changes, and characteristics of the surroundings (distributions of protected area, fishing ground, artificial seagrasses, and shipping traffic). It is needed to assess dispersion of suspended solids, changes of the sea bottom, and impacts on fisheries resources and fishing activities under construction of offshore wind power. Furthermore, the responses of marine organisms to noise and vibration, impacts by electromagnetic fields, impacts on sea birds, hindrances to sea lane routes, and damaged scenery and marine protection areas are thoroughly assessed during operation processes. The consultation criteria in case of development of offshore wind farm is adjusted by focusing marine environmental impact assessment.