Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Effects of Cadmium, Copper, Chromium, Nickel, Silver, and Zinc on the Embryonic Development of the Sea Urchin, Strongylocentrotus intermedius

북쪽말똥성게 (Strongylocentrotus intermedius) 배아 (embryo)를 이용한 중금속에 대한 민감도 비교

  • 류태권 (국립환경과학원 위해성평가과) ;
  • 황인영 (인제대학교 자연과학대학 환경공학부) ;
  • 이택견 (한국해양연구원 남해연구소) ;
  • 윤준헌 (국립환경과학원 위해성평가과) ;
  • 이창훈 ((주)네오엔비즈 부설 환경안전연구소)
  • Published : 2010.03.31
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Abstract

Discharged materials from the point or non-point source are released into the sea, and as the results, marine environment is directly affected. We must estimate the impacts of contaminants to marine pollution rapidly and accurately. Therefore, it is needed on early warning system for appreciating marine environmental impacts, and required a bioassay to evaluate abnormal changes. A bioassay test was developed to examine the effects of heavy metal contaminants on the early life stages of the marine annimals. We have studied the effects of metals on early development of a sea urchin species, Strongylocentrotus intermedius. S. intermedius embryos were tested with six metals (Cu, Ag, Zn, Cd, Cr, Ni) and showed the highest sensitivity to Cu as well as the lowest sensitivity to Cd. The order of biological impact for metals was Cu>Ag>Ni>Zn>Cr>Cd. In accordance with the results, sea urchins embryos can provide biological criteria for seawater quality assessment. The sensitivity of developmental bioassay whith S. intermedius is at intermediate level among marine organisms commonly used in aquatic bioassays. And this sea urchin can be routinely employed as a test organism for ecotoxicity assays.

Keywords

References

  1. Anne Bottger S and James B. McClintock. The effects of organic inorganic phosphates on fertilization and early development in the sea urchin Lytechinus variegatus (Echinodermata: Echinoidea). Compara. Biochem. and Physio. Part C: Toxicol and Pharm 2001; 129(4): 307-315. https://doi.org/10.1016/S1532-0456(01)00190-9
  2. ASTM. Standard guide for conducting static acute toxicity tests with echinoid embryos. E1563-95, American Society for Testing and Materials, Philadelphia, PA, 1995; 19 pp.
  3. Beiras R and Albentosa M. Inhibition of embryo development of the commercial bivalves Ruditapes decussatus and Mytilus galloprovincialis by trace metals; Implication for the implementation of seawater quality criteria. Aquacul 2004; 230: 205-213. https://doi.org/10.1016/S0044-8486(03)00432-0
  4. Bellas J, Beiras R and Vazquez E. A standardisation of Ciona intestinalis (Chordata, Ascidiacea) embryo-larval bioassay for ecotoxicological studies. Water Res 2003; 37(19): 4613-4622. https://doi.org/10.1016/S0043-1354(03)00396-8
  5. Calevro F, Campani S, Ragghianti M, Bucci S and Mancino G. Test of toxicity and teratogenicity in biphasic vertebrates treated with heavy metals $(Cr^{3+}, Al^{3+}, Cd^{2+})$. Chemosphe 1998; 37: 3011-3017. https://doi.org/10.1016/S0045-6535(98)00342-7
  6. Chapman GA. Sea urchin fertilization test method, Newport, OR; U.S. EPA, ERL-Narragansett, Pacific Ecosystem Branch 1992; 1-35.
  7. Cherr GN, Shenker JM, Lundmark C and Turner KO. Toxic effects of selected bleached kraft mill effluent constituents on the sea urchin sperm cell. Environ Toxicol Chem 1987; 6: 561-569. https://doi.org/10.1002/etc.5620060708
  8. Damasio J, Tauler R, Teixido E, Rieradevall M, Prat N, Riva MC, Amadeu Soares MVM and Barata C. Combined use of Daphnia magna in situ bioassays, biomarkers and biological indices to diagnose and identify environmental pressures on invertebrate communities in two Mediterranean urbanized and industrialized rivers (NE Spain). Aqua Toxico 2008; 87: 310-320. https://doi.org/10.1016/j.aquatox.2008.02.016
  9. Dinnel PA, Link JM, Stober QJ, Letourneau MW and Roberts WE. Comparative sensitivity of sea urchin sperm bioassays to metals and pesticides. Arch Environ Contam Toxicol 1989; 18: 748-755. https://doi.org/10.1007/BF01225012
  10. Dinnel PA, Stober QJ, Link JM, Letourneau MW, Roberts WE, Felton SP and Nakatani RE. Methodology and validation of a sperm cell toxicity test for testing toxic substances in marine waters. Final report Grant R/Tox-1, University of Washington, Seattle, WA. 1983; 208 pp.
  11. Geffard O, His E, Budzinski H, Seaman M and Garrigues P. Qualite biologique de l'eau de mer evaluee in situ par le test embryo-larvaire de Crassostrea gigas et Mytilus galloprovincialis: Comptes Rendus de l'Academie des Sciences, Serie III. Sciences de la Vie 2001; 324(12): 1149-1155.
  12. Girotti S, Elida NF, Maria GF and Elisabetta M. Monitoring of environmental pollutants by bioluminescent bacteria. Analytica Chimica Acta 2008; 608(1): 2-29. https://doi.org/10.1016/j.aca.2007.12.008
  13. Gulley DD and WEST, Inc. TOXSTAT 3.5. WEST Inc., Cheyenne, WY. 1996.
  14. Hamilton MA, Russo RC and Thurston RV. Trimmed Spearman-Karber method for estimating median lethal concentration in toxicity bioassays. Environ Sci and Tech 1977; 11: 714-719. https://doi.org/10.1021/es60130a004
  15. His E, Heyvang I, Geffard O and Montaudouin X. A comparison between oyster (Crassostrea gigas) and sea urchin (Paracentrotus lividus) larval bioassays for toxicological studies. Water Res 1999; 33(7): 1706-1718. https://doi.org/10.1016/S0043-1354(98)00381-9
  16. Kobayashi N and Okamura H. Effects of heavy metals on sea urchin embryo development. 1. Tracing the cause by the effects. Chemosphe 2004; 55(10): 1403-1412. https://doi.org/10.1016/j.chemosphere.2003.11.052
  17. Landis WG, Hughes JS and Lewis MA. Status and applications of echinoid (Phylum Echinodermata) toxicity test methods. Environmental Toxicology and Risk Assessment, ASTM STP 1179, Philadelphia 1993; 281-302.
  18. Manzo S. Sea urchin embryo toxicity test; proposal for a simplified bioassay. Ecotoxic Environ Saf 2004; 57(2): 123-128. https://doi.org/10.1016/j.ecoenv.2003.10.007
  19. Martin M, Osborn KE, Bilig P and Glicksten N. Toxicities of ten metals to Crassostrea gigas and Mytilus edulis embryos and Cancer magister larvae. Mar Pollu Bull 1981; 12(9): 305-308. https://doi.org/10.1016/0025-326X(81)90081-3
  20. Nacci D, Jackim E and Walsh R. Comparative evaluation of three rapid marine toxicity test: Sea urchin early embryo growth test, sea urchin sperm cell toxicity test and Microtox. Environ Toxicol Chem 1986; 5: 521-526. https://doi.org/10.1002/etc.5620050603
  21. OECD. Detailed review paper on aquatic testing methods for pesticides and industrial chemicals. OECD series on testing and assessment, number 11. ENV/MC/CHEM (98) 1998; 19/PART1, 260 pp.
  22. Ramachandran S, Patel TR and Colbo MH. Effect of copper and cadmium on three Malaysian tropical estuarine invertebrate larvae. Ecotoxic Environ Saf 1997; 36: 183-188. https://doi.org/10.1006/eesa.1996.1508
  23. Ringwood HA. Comparative sensitivity of gametes and early developmental stages of a sea urchin species (Echinometra mathei) and a bivalve species (Isognomon californicum) during metal exposures. Arch Environ Contam Toxicol 1992; 22: 265-288.
  24. Rumbold DG and Snedaker SC. Evaluation of bioassays to monitor surface microlayer toxicity in tropical marine waters. Arch Environ Contam Toxicol 1997; 32(2): 135-140. https://doi.org/10.1007/s002449900165
  25. Ryu TK, Sung CG, Han GM, Hwang IY, Lee TK and Lee CH. Optimal condition for the embryonic development of sea urchin, Strongylocentrotus intermedius for using the bioassay. J Environ Toxicol 2007; 22(3): 211-218.
  26. Soupilas A, Papadimitriou CA, Samaras P, Gudulas K and Petridis D. Monitoring of industrial effluent ecotoxicity in the greater Thessaloniki area. Desalina 2008; 224(1-3): 261-270. https://doi.org/10.1016/j.desal.2007.07.003
  27. Sung CG, Kim GB, Seo JY, Lee CH, Ryu TK, Han GM, Choi JW and Kim YH. Optimal conditions for the embryonic development of mussel, Mytilus galloprovincialis. Korean J of Malaco 2006; 21(1): 25-31.
  28. Thatheyus AI and Selvanayagam M. Toxic effects of heavy metal nickel in the kidney of common carp, Cyprinus carpio var communis (L.). Converg 1999; 3: 99-102.
  29. USEPA. Short-term methods for estimating the chronic toxicity of effluents and receiving waters to west coast marine and estuarine organisms. EPA/600/R-95/136 1995; pp. 389-465.
  30. Waterman AJ. Effect of salts of heavy metals on development of the sea urchin, arbacia punctulata. Biol Bull 1937; 73(3): 401-420. https://doi.org/10.2307/1537604