• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.2
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• pp.139-145
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• 2009

We cloned and sequenced the open reading frame (ORF) cDNA encoding myostatin from the muscle of abalone (Haliotis discus hannai). The ORF cDNA of the abalone myostatin is 1134 bp and encoded 377 amino acid residues that were 60-96% homologous with the amino acids of other organism myostatins. In addition, the ORF contained a conserved proteolytic cleavage site (RXRR) and nine conserved cysteine residues in the C-terminus. Semi-quantitative RT-PCR revealed the presence of myostatin mRNA in various tissues. The strongest expression was observed in the mantle of female abalone, and the gills and heart of male abalone.

• Journal of Aquaculture
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• v.21 no.3
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• pp.167-171
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• 2008

Common co-existing organism Euplotes (Ciliate, Protozoa) well often observed in the rotifer mass culture tank of marine fish larval rearing stations. Ciliate protozoa (Euplotes) competed to condensed food (micoralgae, Nannochloropsis oculata) with rotifer (Brachionus rotundiformis). As results, rotifer density was rapidly decrease, and what is more, the rotifer was wiped out by dominant bacteria species at that time.

• ALGAE
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• v.21 no.2
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• pp.253-259
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• 2006

Organic substances are released from phytoplankton cells during all phases of growth. The type and amounts of organic substance excreted and the effects of nutrient limitation are often highly species-specific. Dinoflagellate, Cochlodinium polykrikoides grown in batch culture produced an exopolysaccharide. Exopolysaccharide and intracellular polysaccharide concentrations increased as C. polykrikoides cultures progressed from exponential phase, through stationary phase, to declining phase. In the exponential phase, the concentration of exopolysaccharide was relatively low, but in the stationary phase, it showed a rapid increase which seemed to coincide with the depletion of nitrate from the medium. Of the 20 amino acids analyzed, proline dominated in the organic matter of all cultures ranging from 48.2 to 79.9 nmol L–1, and constituting the 20-90% of total amino acids, and followed by histamine varying from 0.7 to 47.5 nmol L–1. Leucine and cysteine were also abundant in the stationary phase. The release rates of exopolysaccharide and intracellualr polysaccharide were higher the end of stationary phase than in the exponential phase. Exopolysaccharide concentration per cell was more than two times higher during the end of stationary phase than that in exponential phase. C. polykrikoides produced extracellular polysaccharide at a rate of 47.04 pg cell–1 day–1.

• Fisheries and Aquatic Sciences
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• v.17 no.4
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• pp.479-486
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• 2014

To evaluate their potential utility as an ornamental organism, novel transgenic marine medaka Oryzias dancena strains with a highly vivid fluorescent phenotype were established through transgenesis of a cyan fluorescent protein gene (cfp) driven by the endogenous fast skeletal myosin light chain 2 gene (mlc2f) promoter. The transgenic marine medaka strains possessed multiple copies of transgene integrants and passed their fluorescent transgenes successfully to subsequent generations. Transgenic expression in skeletal muscles at both the mRNA and phenotypic levels was, overall, dependent upon transgene copy numbers. In the external phenotype, an authentic fluorescent color was dominant in the skeletal muscles of the transgenic fish and clearly visible to the unaided eye. The phenotypic fluorescent color presented differentially in response to different light-irradiation sources; the transgenics displayed a yellow-green color under normal daylight or white room light conditions, a strong green-glowing fluorescence under ultraviolet light, and a cyan-like fluorescence under blue light from a light-emitting diode.

• Journal of Marine Bioscience and Biotechnology
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• v.5 no.4
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• pp.8-14
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• 2011

Although marine living organism contains a numerious number of compounds, it is difficult to collect these compounds in a large scale for medicinal application. However, in recent years, several bioactive compounds isolated from marine macroalgae have been proved to be able to provide potential sources for development of medicinal products because they can be obtained in large amount from marine. A number of studies have reported a variety of effects of marine macroalgae but a few anti-inflammatory activity of marine macroalgae have recently been published. Herein, we reviewed novel anti-inflammatory compounds recently isolated from marine brown algae, green algae and red algae. From this survey, in particular, some compounds contained in edible macroalgae exert anti-inflammatory effects with inhibition on cyclooxygenase-2 (COX-2), inducible nitric oxide synthase(iNOS) and matrix metalloproteinases (MMPs) activity regulated by nuclear factor-kappa B transcription factor that play a key role in cancer as well as inflammation, demonstrating to be able to potentially apply to development of anti-inflammatory agent for chemoprevention of cancer. Furthermore, some macroalgae and their compounds with both excellent anti-inflammatory activity and very low toxicity can select a potential candidates capable of preventing or treating several chronic inflammation such as colitis, hepatitis and gastritis, leading to cancer.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.364-367
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• 2005

This study investigated the production of antioxidant from Actinomyces culture supernatant. For the research of the natural marine antioxidant, several bacteria were isolated from the coast of Je-ju in Korea. An actinomycetes strains, S-1 was identified to a genus level 16S ribosomal DNA sequence and fatty acid analysis. From these results and other characteristics described in the Bergey's Manual, this strain was identificated as a Nocardiopsis dassonvillei. Strain S-1 showed high activity of 1,1-diphenyl-2-prcrylhydrazyl radical scavenging. The hydroxyl radical scavenging ability of Nocardiopsis sp. S-1 supernatant was 53%. Nutritional and cultural conditions for the production of antioxidant by this organism under shake-flask conditions have optimized. Similary initial medium pH 7.6, incubation temperature of $25^{\cicr}C$, sodium chloride concentration 2.5 and incubation time of 8 day were found to be optimal.

• Journal of the Korean Society of Marine Environment & Safety
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• v.29 no.5
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• pp.456-461
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• 2023

The Korea Maritime Environment Corporation is conducting a comprehensive survey of the national marine ecosystem under the commission of the Ministry of Oceans and Fisheries (MOF) to ensure continuous use of the ocean, preserve and manage the marine ecosystem. The survey has set major peaks to investigate changes in the marine ecosystem around the Korean Peninsula. However as the peak has been set around the coast, it is necessary to expand the scope of investigation to encompass offshore areas. Meanwhile, the Aids to Navigation Division of the MOF supports a comprehensive national marine ecosystem survey providing photographs of fouling organisms during the Aids to Navigation lifting inspection, however, the photographs are provided only in consultation with the Korea Maritime Environment Corporation. Therefore, a study was conducted to generate information on fouling organisms using deep learning-based image processing algorithms by the lifting Aids to Navigation and dorsal buoys so that Aids to Navigation could be used as the major component of a comprehensive national marine ecosystem. If the Aids to Navigation are used as the peak of the survey, they could serve as fundamental data to enhance their own value as well as analyze abnormal marine conditions and ecosystem changes in Korea.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.17 no.4
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• pp.262-269
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• 2012

We investigated the concentration levels and evaluated the distributions of 17 polychlorinated dibenzo-p-dioxins and furans(PCDD/Fs), 12 dioxin-like polychlorinated biphenyls(DLPCBs) and 24 polybrominated diphenyl ethers (PBDEs) in sediment and organism from various culturing grounds in Korean coast. The levels of PCDD/Fs, DLPCBs, and PBDEs in sediment samples ranged from 0.03 to 2.9(Mean${\pm}$SD, $1.2{\pm}0.89$) pg $WHO_{2005}$-TEQ $g^{-1}$ dry weight(dw), ND(not detected) to 1.1($0.09{\pm}0.19$) pg $WHO_{2005}$-TEQ $g^{-1}$ dw, and ND to 16.6($2.96{\pm}3.53$) ng $g^{-1}$ dw, respectively. Also, the levels of PCDD/Fs, DLPCBs, and PBDEs in culturing organisms ranged from ND to 0.24($0.07{\pm}0.06$) pg $WHO_{2005}$-TEQ $g^{-1}$ wet weight(ww), ND to 0.11($0.04{\pm}0.04$) pg $WHO_{2005}$-TEQ $g^{-1}$ ww, and 0.05 to 0.29($0.13{\pm}0.06$) ng $g^{-1}$ ww, respectively. The levels of total dioxins(PCDD/Fs+DLPCBs) and PBDEs in sediments from East and South sea were four to seven times higher than those from West sea. PCDD/Fs had dominant contribution(mean, 94%) for TEQ concentration in sediment, whereas relatively higher contribution of DLPCBs(33%) were shown in culturing organism than sediment. BDE209 was a dominant congener in both matrix. The concentrations of PCDD/Fs, DLPCBs and PBDEs were in decreasing trends in Korea coasts compared to previous results.

• Environmental Analysis Health and Toxicology
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• v.20 no.4 s.51
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• pp.333-341
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• 2005

In order to know whether polychaetes could be used as an appropriate organism for the detection of genotoxicity, DNA strand breaks were evaluated in blood cells of a nereidae worm (Perinereis aibuhitensis) exposed to various aquatic chemical pollutants (e.g. Cd, Pb, Pyrene, Benaor[a]pyrene). Hydrogen peroxide increased DNA strand breaks up to the highest concentration (10 $\mu$M). Higher concentration than 0.1 $\mu$M showed a significantly more DNA damage than control. Cadmium and lead also showed higher DNA damage than control, over 1.0 and 1 $\mu$g/L, respectively. In case of pyrene, DNA damage was detected even at 0.001 $\mu$g/L. However, DNA damage decreased due to apoptosis at the highest concentration of pyrene and Pb. This study suggested that the polythaetous blood cells could be used effectively for screening genotoxic contaminants in the environment.

• Fisheries and Aquatic Sciences
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• v.20 no.11
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• pp.28.1-28.17
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• 2017

Members of the phylum Echinodermata, commonly known as echinoderms, are exclusively marine invertebrates. Among the Echinodermata, sea cucumber belongs to the family Holothuroidea. The sea cucumber Stichopus (Apostichous) japonicus (Selenka) is an invertebrate animal inhabiting the coastal sea around Korean, Japan, China, and Russia. Sea cucumber has a significant commercial value, because it contains valuable nutrients such as vitamins and minerals. They possess a number of distinctive biologically and pharmacologically important compounds. In particular, the body wall of sea cucumber is a major edible part. It consists of peptide, collagen, gelatin, polysaccharide, and saponin, which possess several biological activities such as anti-cancer, anti-coagulation, anti-oxidation, and anti-osteoclastogenesis. Furthermore, the regenerative capacity of sea cucumber makes it a medically important organism. This review presents the various biological activities and biomedical potential of sea cucumber S. japonicus.