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Evaluation of Genotoxicity in Blood Cells of a Polychaetous Worm (Perinereis aibuhitensis), Using Comet Assay  

Seo Jin Young (Department of Marine Environmental Engineering, Marine Industry Institute, Gyeongsang National University)
Sung Chan Gyoung (Department of Marine Environmental Engineering, Marine Industry Institute, Gyeongsang National University)
Choi Jin Woo (South Sea Institute, KORDI)
Lee Chang Hoon (South Sea Institute, KORDI)
Ryul Tae Kwon (South Sea Institute, KORDI)
Han Gi Myung (South Sea Institute, KORDI)
Kim Gi Beum (Department of Marine Environmental Engineering, Marine Industry Institute, Gyeongsang National University)
Publication Information
Environmental Analysis Health and Toxicology / v.20, no.4, 2005 , pp. 333-341 More about this Journal
Abstract
In order to know whether polychaetes could be used as an appropriate organism for the detection of genotoxicity, DNA strand breaks were evaluated in blood cells of a nereidae worm (Perinereis aibuhitensis) exposed to various aquatic chemical pollutants (e.g. Cd, Pb, Pyrene, Benaor[a]pyrene). Hydrogen peroxide increased DNA strand breaks up to the highest concentration (10 $\mu$M). Higher concentration than 0.1 $\mu$M showed a significantly more DNA damage than control. Cadmium and lead also showed higher DNA damage than control, over 1.0 and 1 $\mu$g/L, respectively. In case of pyrene, DNA damage was detected even at 0.001 $\mu$g/L. However, DNA damage decreased due to apoptosis at the highest concentration of pyrene and Pb. This study suggested that the polythaetous blood cells could be used effectively for screening genotoxic contaminants in the environment.
Keywords
comet assay; polychaetous blood cell; bioassay; metal; PAH;
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