• Ocean Science Journal
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• v.42 no.4
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• pp.247-254
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• 2007

Marine bacteria, actionmycetes and fungal strains were isolated from continental slope sediment of the Bay of Bengal and studied for fatty acid profile to investigate their involvement in the benthic food-web. Fifteen different saturated and unsaturated fatty acids from bacterial isolates, 14 from actinomycetes and fungal isolates were detected. The total unsaturated fatty acids in bacterial isolates ranged from 11.85 to 37.26%, while the saturated fatty acid ranged between 42.34 and 80.74%. In actinomycetes isolates, total unsaturated fatty acids varied from 27.86 to 38.85% and saturated fatty acids ranged from 35.29 to 51.25%. In fungal isolates unsaturated fatty acids ranged between 44.62 and 65.52% while saturated FA ranged from 20.80 to 46.30%. The higher percentages of unsaturated fatty acids from the microbial isolates are helpful in anticipating the active participation in the benthic food-web of Bay of Bengal.

• Korean Journal of Ichthyology
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• v.26 no.1
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• pp.74-80
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• 2014

Seasonal variation in species composition and abundance of fish assemblage in the tidal creek of Sangnae-ri Suncheon, Korea were studied using monthly samples collected by a push net from April 2011 to March 2012. During the study period, a total of 1,095 individuals belonging to 13 species in 6 families were collected. The dominant fish species were Tridentiger bifasciatus, Mugil cephalus and Favonigobius gymnauchen. These three fish species accounted for 86.6% of the total number of individuals collected. The number of fish species, number of individuals, biomass fluctuated with season showing a low value in winter and high value in spring.

• Journal of fish pathology
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• v.23 no.3
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• pp.323-334
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• 2010

Bactericidal/permeability-increasing protein (BPI) and lipopolysaccharide-binding protein (LBP) are important components of the mammalian innate defence system against Gram-negative infections. The BPI/LBP cDNA was identified from the black rockfish ConA/PMA or LPS stimulated leukocyte cDNA library. The full-length BR-BPI/LBP cDNA was 2118 bp long and contained an open reading frame (ORF) of 1422 bp that encoded 473 amino-acid residues. The 5' UTR had a length of 57 bp, and the 3' UTR 639 bp. The molecular weight and theoretical isoelectric point (pI) values were calculated 51.4 kDa and 9.72, respectively. Compared with other known BPI or BPI/LBP peptide sequences, the most conserved regions of the black rockfish BPI/LBP peptide were found to be the BPI1 N-terminal, BPI2 C-terminal domains and a LPS binding domain. Phylogenetic analysis based on the deduced amino acid sequence revealed a homologous relationship between the BPI/LBP sequence of black rockfish and that of other teleosts. The black rockfish BPI/LBP gene was predominantly expressed in the PBLs, head kidney, trunk kidney and spleen. The expression of the black rockfish BPI/LBP molecule was induced in the peripheral blood leukocytes (PBLs) from 1 to 24 h following LPS stimulation, with a peak at 12 h post-stimulation.

• Journal of fish pathology
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• v.26 no.3
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• pp.231-240
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• 2013

Adjuvants are immune enhancers that are often used in vaccination to augment the immune response of a vaccine, thereby enhancing the protective immunity against the targeted disease. In the present study, we used the recombinant protein, such as rRbCC1, this protein was produced from rock bream CC chemokine 1. To verify the adjuvant effects of this recombinant protein, the immune responses of rock bream to Streptococcus iniae (S. iniae) FKC vaccination, which alone or in combination with recombinant protein was analyzed and then also performed experimental challenge with live S. iniae. The result of serum agglutination titres was showed relatively low levels however, the efficacy of FKC vaccine still conferred protection against S. iniae. Moreover, the adverse effects result showed that no statistically significant difference was revealed between high concentration injected and non-injected fish groups, generally. The relative percent survival (RPS) of FKC + recombinant vaccination group was significantly higher than that of vaccinated group with FKC alone. After experimental challenge to the rock bream by injection with live bacteria (S. iniae), the FKC + rRbCC1 vaccination group was showed 87.0% RPS, however, the RPS of FKC alone vaccination was 68.2%. The results indicated that the recombinant protein as an adjuvant had a clear synergism to injection vaccine of rock bream.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.6
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• pp.1000-1006
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• 2021

The maturity and spawning of the common octopus Octopus vulgaris were studied using 317 samples collected monthly from January to December of 2020 from the coastal waters of Yeosu, Korea. The mantle length (ML) of O. vulgaris ranged from 5.1 to 19.7 cm and body weight (BW) ranged from 117.6 to 3,645.4 g. We analyzed monthly changes in sex ratio, gonadosomatic index (GSI), histological maturity stage, and body weight at 50%, 75% and 97.5% group maturity. The sex ratio was 1:1.02, which was not significantly different from the value that expected. Based on histological observation of the gonad development and gonadosomatic index, the spawning periods lasted throughout the year, with peak spawning periods estimated from March to April and July to August. The percentages of sexually mature females estimated using a logistic equation were over 50% at 554.46 g BW, 75% at 1,134.38 g BW and 97.5% at 2,474.22 g BW respectively.

• Fisheries and Aquatic Sciences
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• v.15 no.2
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• pp.177-181
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• 2012

To understand the effects of lower pH levels due to elevated $CO_2$ and salinity, we designed and constructed a pH-control system that included automatic $CO_2$ infusion and measured the hatching rate of a crustacean model species, Artemia franciscana. The pH-control system was cost-effective and capable of performing animal tests in which pH fluctuated around $8.0{\pm}0.1$, with the temperature around $27{\pm}0.5^{\circ}C$. Hatching rate was observed under four different pH levels (7.0, 7.3, 7.6, and untreated control) combined with three salinity ranges (15, 25, and 35 ppt). The results demonstrated that lower pH levels led to decreased hatching rates regardless of salinity, and the minimum hatching rate was detected at pH 7.0 compared to the control (pH $8.0{\pm}0.1$), supporting the idea that OA has adverse effects on hatching rates and increases the risk of juveniles being introduced in the ecosystem. In contrast, salinity changes exhibited no synergistic effects with pH and had independent effects.

• Korean Journal of Environmental Biology
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• v.36 no.3
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• pp.308-313
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• 2018

During a survey of marine algal flora, a red algal species was collected from Giseong, Uljin located on the eastern coast of Korea. This species has the generic features of Cryptonemia belonging to Halymeniaceae, and is characterized by the presence of erect foliose thalli arising from a discoid holdfast, somewhat fan-shaped blade with an evanescent midrib at the base, narrow main axes with blade-like wings of slightly undulate margin, a perennial stalk, and entwined filamentous medulla with refractive stellate cells. In a phylogenetic tree based on rbcL sequence, the Korean alga nests in the same clade with C. lomation from France and C. seminervis from Spain. Genetic divergence among the sequences within the clade was not recognized thus suggesting that both the species are conspecific. The name C. lomation considered to be valid nomenclaturally is accepted for the entity. Based on the morphological and molecular analyses, the Korean alga is identified as C. lomation, originally described from Italy. This confirms the occurrence of C. lomation in Korea. The species appears to be distributed in the temperate region influenced more or less by the North Korea Cold Current.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2004.10a
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• pp.68-68
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• 2004

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2006.06a
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• pp.7-8
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• 2006

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2004.12a
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• pp.62-62
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• 2004