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http://dx.doi.org/10.7847/jfp.2013.26.3.231

The immune-adjuvant effect and safety of recombinant CC chemokine 1 (rRbCC1) in rock bream, Oplegnathus fasciatus  

Kwon, Mun-Gyeong (Pathology Division, National Fisheries Research and Development Institute)
Kim, Ju-Won (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University)
Hwang, Seong-Don (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University)
Kim, Eun-Gyeong (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University)
Park, Dae-Won (Gyeongsangnam-do Fisheries Resources Research Institute)
Park, Chan-Il (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University)
Publication Information
Journal of fish pathology / v.26, no.3, 2013 , pp. 231-240 More about this Journal
Abstract
Adjuvants are immune enhancers that are often used in vaccination to augment the immune response of a vaccine, thereby enhancing the protective immunity against the targeted disease. In the present study, we used the recombinant protein, such as rRbCC1, this protein was produced from rock bream CC chemokine 1. To verify the adjuvant effects of this recombinant protein, the immune responses of rock bream to Streptococcus iniae (S. iniae) FKC vaccination, which alone or in combination with recombinant protein was analyzed and then also performed experimental challenge with live S. iniae. The result of serum agglutination titres was showed relatively low levels however, the efficacy of FKC vaccine still conferred protection against S. iniae. Moreover, the adverse effects result showed that no statistically significant difference was revealed between high concentration injected and non-injected fish groups, generally. The relative percent survival (RPS) of FKC + recombinant vaccination group was significantly higher than that of vaccinated group with FKC alone. After experimental challenge to the rock bream by injection with live bacteria (S. iniae), the FKC + rRbCC1 vaccination group was showed 87.0% RPS, however, the RPS of FKC alone vaccination was 68.2%. The results indicated that the recombinant protein as an adjuvant had a clear synergism to injection vaccine of rock bream.
Keywords
Rock bream; Adjuvant; Recombinant protein; Vaccine; Streptococcus iniae;
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1 Aakre, R., Wergeland, H.I., Aasjord, P.M., Endresen, C.: Enhanced antibody response in Atlantic salmon (Salmo salar L.) to Aeromonas salmonicida cell wall antigens using a bacterin containing $\beta$- 1,3-M-glucan as adjuvant. Fish Shellfish Immunol., 4:47-61, 1994.   DOI   ScienceOn
2 Agnew, W., Barnes, A.C.: Streptococcus iniae: an aquatic pathogen of global veterinary significance and a challenging candidate for reliable vaccination. Vet. Microbiol., 122:1-15, 2007.   DOI   ScienceOn
3 Altman, A., Dixon, F.J.: Immunomodifiers in vaccines. Adv. Vet. Sci. Comp. Med., 23:301-41, 1989.
4 Baeck, G.W., Kim, J.H., Gomez, D.K., Park, S.C.: Isolation and characterization of Streptococcus sp. from diseased flounder (Paralichthys olivaceus) in Jeju island. J. Vet. Sci., 7:53-8, 2006.   과학기술학회마을   DOI   ScienceOn
5 Bercovier, H., Ghittino, C., Eldar, A.: Immunization with bacterial antigens: infections with streptococci and related organisms. Dev. Biol. Stand., 90:153-60, 1997.
6 Berg, A., Rodseth, O.M., Hansen, T.: Fish size at vaccination influence the development of side-effects in Atlantic salmon (Salmo salar L.). Aquaculture, 265:9-15, 2007.   DOI   ScienceOn
7 Cataldi, E., Marco, P.D., Mandich, A., Cataudella, S.: Serum parameters of Adriatic sturgeon Acipenser naccarii (Pisces: Acipenseriformes): effects of temperature and stress. Comp. Biochem. Physiol. Part A, 121:351-4, 1998.   DOI   ScienceOn
8 Cho, M.Y., Lee, D.C., Lee, J.S., Do, J.W., Kim, M.S., Choi, M.Y., Kim, Y.C., Kang, B.K., Yoon, Y.D., Kim, J.W.: Stability and efficacy of formalin-killed Streptococcus iniae vaccine for olive flounder, Paralichthys olivaceus. J. Fish pathol., 19:165-72, 2006.   과학기술학회마을
9 Chu, W.H.: Adjuvant effect of propolis on immunisation by inactivated Aeromonas hydrophila in carp (Carassius auratus gibelio). Fish Shellfish Immunol., 21:113-7, 2006.   DOI   ScienceOn
10 Cox, J.C., Coulter, A.R.: Adjuvants-a classification and review of their modes of action. Vaccine, 15:248-56, 1997.   DOI   ScienceOn
11 Freund, J., Casals, J., Hosmer, E.P.: Sensitization and antibody formation after injection of tubercle bacilli and paraffin oil. Proc. Soc. Exp. Biol. Med., 37:509, 1937.   DOI
12 Hastein, T., Gudding, R., Evensen, O.: Bacterial vaccines for fish--an update of the current situation worldwide. Dev. Biol (Basel)., 121:55-74, 2005.
13 Horne, M.T., Roberts, R.J.,Tatner, M., Ward, P.: The effects of the use of potassium alum adjuvant in vaccines against vibriosis in rainbow trout, Salmo gairdneri Richardson. J. Fish Dis., 7:91-9, 1984.   DOI
14 Jiao, X.D., Cheng, S., Hu, Y.H., Sun, L.: Comparative study of the effects of aluminum adjuvants and Freund's incomplete adjuvant on the immune response to an Edwardsiella tarda major antigen. Vaccine, 28:1832-7, 2010.   DOI   ScienceOn
15 Lofthouse, S.A., Andrew, A.E., Nash, A.D., Bowles, V.M.: Humoral and cellular responses induced by intradermally administered cytokine and conventional adjuvants. Vaccine, 13:1131-7, 1995.   DOI   ScienceOn
16 Jung, S.H., Byun, S.G., Jee, B.Y., Choi, H.S.: Application of veterinary chemistry analyzer used to hematological analysis of marine fish cultured in floating netcage. J. Fish pathol., 19:253-65, 2006.   과학기술학회마을
17 Kim, J.W., Kim, E.G., Kim, D.H., Shim, S.H., Park, C.I.: Molecular identification and expression analysis of the CC chemokine gene in rock bream (Oplegnathus fasciatus) and the biological activity of the recombinant protein. Fish Shellfish Immunol., 34:892-901, 2013.   DOI   ScienceOn
18 Lindblad, E.B.: Aluminium compounds for use in vaccines. Immunol. Cell. Biol., 82:497-505, 2004.   DOI   ScienceOn
19 McElrath, M.J.: Selection of potent immunological adjuvants for vaccine construction. Semin. Cancer Biol., 6:375-85, 1995.   DOI   ScienceOn
20 Mulvey, B., Landolt, M.L., Busch, R.A.: Effects of potassium sulphate (alum) used in Aeromonas salmonicida bacterin on Atlantic salmon, Salmo salar L. J. Fish Dis., 18:495-506, 1995.   DOI
21 Mutoloki, S., Alexandersen, S., Evensen, O.: Sequential study of antigen persistence and concomitant inflammatory reactions relative to side-effects and growth of Atlantic salmon (Salmo salar L.) following intraperitoneal injection with oil-adjuvanted vaccines. Fish Shellfish Immunol., 16:633-44, 2004.   DOI   ScienceOn
22 Mutoloki, S., Alexandersen, S., Gravningen, K., Evensen, O.: Time-course study of injection site inflammatory reactions following intraperitoneal injection of Atlantic cod (Gadus morhua L.) with oil-adjuvanted vaccines. Fish Shellfish Immunol., 24:386-93, 2008.   DOI   ScienceOn
23 Rorstad, G., Aasjord, P.M., Robertsen, B.: Adjuvant furunculocis in Atlantic salmon (Salmo salar L.). Fish Shellfish Immunol., 3:179-90, 1993.   DOI   ScienceOn
24 Park, S.I.: Disease control in Korean aquaculture. Fish Pathol., 44:19-23, 2009.   DOI
25 Poppe, T.T., Breck, O.: Pathology of Atlantic salmon Salmo salar intraperitoneally immunized with oil-adjuvanted vaccine. A case report. Dis. Aquat. Organ., 29:219-26, 1997.   DOI
26 Robertsen, B., Rerstad, G., Engstad, R.: Enhancement of non-specific disease resistance in Atlantic salmon, Salmo salar L., by a glucan from Saccharomyces cerevisiae cell walls. J. Fish Dis., 13:391-400, 1990.   DOI
27 Scheerlinck, J.Y.: Genetic adjuvants for DNA vaccines. Vaccine, 19:2647-56, 2001.   DOI   ScienceOn
28 Shi, X.J., Wang, B., Wang, M.: Immune enhancing effects of recombinant bovine IL-18 on foot-and-mouth disease vaccination in mice model. Vaccine, 25:1257-64, 2007.   DOI   ScienceOn
29 Shutou, K., Kanai, K., Yoshikoshi, K.: Role of capsule in immunogenicity of Streptococcus iniae to Japanese flounder Paralichthys olivaceus. Fish Pathol., 42:101-6, 2007.   DOI
30 Singh, M., O'Hangan, D.: Advances in vaccine adjuvant. Nat. Biotechnol., 17:1075-81, 1999.   DOI   ScienceOn
31 Sommerset, I., Krossoy, B., Biering, E., Frost, P.: Vaccines for fish in aquaculture. Expert Rev. Vaccines, 4:89-101, 2005.   DOI   ScienceOn
32 Sun, Y., Liu, C.S., Sun, L.: A multivalent killed whole-cell vaccine induces effective protection against Edwardsiella tarda and Vibrio anguillarum. Fish Shellfish immunol., 31:595-9, 2011.   DOI   ScienceOn
33 Williams, D.L., Yaeger, R.G., Pretus, H.A., Browder, I.W., McNamee, R.B., Jones, E.L.: Immunization against Trypanosoma cruzi: adjuvant effect of glucan. Int. J. Immunopharmacol., 11;403-10, 1989.   DOI   ScienceOn