• Applied Microscopy
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• v.30 no.3
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• pp.303-310
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• 2000

Ultrastructures on the integumentary epidermis of the marbled sole, Limanda yokahamae, were examined by means of the light and transmission electron microscope. Epidermal layer consists of supporting cells, unicellular glands and accessory cells. The supporting cells were classified into superficial cell, intermediated cell and basal cell. The cytoplasm of supporting cells is divided into cortex and medullar part. In the cortex and medullar part, microfilaments and cell organelles are well developed, respectively. Gland cells are present in the superficial and middle epidermis. The cytoplasm of mucous cell reacted to blue in AB-PAS (pH 2.5). Club cell has a roundish central vacuole and well-developed microfilaments in the cytoplasm. Granular cells are occurs in the middle and basal epidermis , and the cytoplasm is occupied with membrane-bounded granules of electron dense. Chloride cells are present in the superficial epidermis , and the cytoplasm is occupied with tubular mitochondria. Three types of pigment cells can be distinguished by electron density of cytoplasmic inclusions.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.26 no.4
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• pp.309-316
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• 1990

Age and growth of the Marbled Sole Limanda Yokohamae in Tokyo Bay were studied by otolith reading of 1,070 fishes from May 1986 to April 1988. Examination of the outer margins of the otoliths showed that the hyaline zone as annulus was formed once a year and that its formation was completed at the end of the spawning season (Feb. to Apr.). Growth of the fish was expressed by the von Bertallinffy's equaion as Lt=255.7{1-exp[-0.505(t-0.149)]} for males and Lt=376.9{1-exp[-0.303(t-0.202)]} for females, where Lt is standard length in mm and t is age in years. It was found that the growth of males and females differs, with the females showing a higher growth than the males at each estimated age. Growth in body weight was also examined. Most of the fish examined were found to be not more than three years old, although the oldest fish were four years old for males and seven years old for females.

• Journal of fish pathology
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• v.24 no.3
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• pp.213-223
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• 2011

Among wild marbled sole, Pleuronectes yokohamae caught in west coastal area, Chungnam, tumor-bearing fish were found. Parasites and pathogenic bacteria were not detected and CPE was not observed. Using the light microscope, the epidermal layer of wild P. yokohamae was significantly thickened compared to the normal skin. The lesion was formed papillary folds. Hypertropical epithelial cells revealed karyolysis, marked nucleolus and cloud swelled cytoplasm. In the epidermal layer of the lesion, X-cell that is characterized by oval and small pale nucleus and prominent nucleolus was observed. Dermal layer had newly formed vessels. The size of mucous cell in the papilloma lesion was significantly increased compared to the normal. In this study, no pathogens were found, so future works for finding cause of the papilloma in the P. yokohamae are needed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.6
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• pp.558-562
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• 1986

The purpose of this study is to find the light intensity which induces maximum gathering rate and to observe the variation of the gathering rate both in daytime and at night by using marbled sole, Limanda yokohamae (Gunther). An experimental tank ($360L{\times}50W{\times}55H\;cm$) was set up in a dark room. An illumination system was attached to the end of one side of the tank to control horizontal light intensity. Six artificial light sources were prepared by combination of two light bulbs (5W, 150W) and seven filters. During the experiment water depth was maintained 50 cm level in the tank. The tank was marked into six longitudinal sections each being 60 cm long to observe the distribution of fish. The fish were acclimatized in dark condition for 50 minutes prior to the main experiment. Upon turning on the light, the number of fish in each section was counted 40 times every 30 seconds, and the gathering rate was obtained from the average number of fish in each section. The light intensity inducing maximum gathering rate was 1.9 lux ($1.2{\sim}2.9\;lux$) in the daytime and 0.7 lux ($0.5{\sim}1.1\;lux$) at night. The variation of the gathering rate of fish in illumination time was small and comparatively fluctuated with stability.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.33 no.2
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• pp.85-89
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• 1997

한국서해안 격열비열도 근해산 문치가자미의 연령과 성장을 조사하기 위하여 1992년 9월부터 1993년 8월까지 매월 정기채집한 256개체의 이석을 연령형질로서 사용하여 조사 분석한 결과를 보면, 이석의 투명대와 불투명대는 년 1회 형성되었으며 투명대 형성시기는 7월이고 불투명대 형성 시기는 2월에서 4월로 이는 산란종기(2월)와 일치함을 보였다. 연령별 체장 (LT)과 연령(t)을 von Bertalanffy 성장식에 적용하여 수컷은 Lt=313.7[1-exp{-0.4342(t+0.9017)}, 암컷은, Lt=458.1[1-exp{-0.2368(t+0.1934)}과 같은 관계식을 구하였다. 또한 암컷과 수컷의 성장속도는 2세까지는 별다른 차이를 보이지 않았으나, 3세어 이상에서는 암컷이 수컷보다 월등히 빠름을 나타내었다. 본 연구에서의 최고령어는 암수 각각 5세와 6세였다.

• Korean Journal of Ichthyology
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• v.13 no.3
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• pp.161-165
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• 2001

Artificial fertilization (dry method) of mature right-eye flounder, Limanda yokohamae (female : 25.1~30.4 cm in total length (TL), male : 24.5~28.5 cm in TL), obtained from the fish market in Dolsan-do, Yosu was performed in the Fisheries Exhibition. Hatched larvae and juveniles were reared to describe their morphological development. Newly hached larvae attained 3.13~3.42 mm in TL. Their eyes were yellowish brown and their mouth and anus were not yet opened. Three to six days after hatching the larvae attained 3.35~4.61 mm in TL. Their mouth and anus were open and the yolk sac was almost absorbed. Feeding activity increased as the mouth became larger. At 25 days, the larvae attained 5.47~5.91 mm in TL. The caudal notocord was flexed $45^{\circ}$ upward. At 35 days, the larvae attained 6.83~7.60 mm in TL. Rays of the dorsal and anal fins were formed, and the left eye was moved slightly to the right side of the head. At 55 days, the juveniles attained 9.38~11.73 mm in TL. The left eye was moved completely onto the right side. All of the fins had complete set of the fin rays, and the juveniles spent most of the time on the bottom resting on their blind side (D. 68~70 : A. 50~52 : P. 11: V. 6 : C. 18~19).

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.49 no.4
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• pp.352-359
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• 2013

To estimate the mesh selectivity of gillnet for ocellate spot skate (Okamejei kenojei), the field tests were carried out 12 times with six different mesh sizes (121.2mm, 137.7mm, 151.5mm, 168.3mm, 178.2mm, 189.4mm) in the coastal waters of Taean, Chungcheongnamdo of Korea, 2010~2011. In the field tests, the total number of species was 31, and that of catch was 1,410 and the total weight was 618,006g. The number and weight of ocellate spot skate which is main target in this study were 1,004 and 434,592g, respectively. The catch in number of ocellate spot skate occupied about 71.2% in total catch. The others of catch species were marbled sole (8.4%), sea raven (4.4%), japanese swimming crab (4.2%) and flatfish (4.1%) and so on. The range of body disk width (DW) of ocellate spot skates which were caught in this study was 15.2~35cm and the mode was 27~29cm. The estimation equation of mesh selectivity using the extended Kitahara's method was expressed as s $(R)=s(DW/m)={\exp}\{(-0.56R^3-1.80R^2+12.96R-9.99)-4.26\}$. The optimum value of DW/m for 1.0 of retention probability in this estimation equation was estimated 1.899 and DW/m was estimated to be 1.194, 1.314, 1.395, 1.461 and 1.520 when the retention probability were 0.1, 0.2, 0.3, 0.4 and 0.5, respectively. When applied to the retention probability of 0.5, the optimum mesh size was estimated to be 177.0mm on first maturity disk width 26.9cm of ocellate spot skate.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.3
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• pp.261-265
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• 1985

Limanda yokohamae was studied on its growth, age at maturity, fecundity and survivorship, based on the specimens off the southeastern coast of Korea from December 1983 to November 1984. The total lengths at the formation of annulus on the otolith were back-calculated. The age at the first reproduction is 2yrs in both sexes. Fecundity was estimated to be $121{\times}10^3{\sim}429{\times}10^3$. Annual survival rate is 0.435 in female and 0.335 in male.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.49 no.2
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• pp.106-115
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• 2013

A series of fishing experiments were carried out in the western coastal waters of Korea from May, 2006 to September, 2007, using gill nets of different mesh sizes (m=101, 121.8, 152.5, 176.8, and 191 mm) to determine the mesh selectivity of gill net for swimming crab, Potunus trituberculatus. The catch species was composed of swimming crab (87%), Thomas's rapa whelk Rapana venosa (5%), arthritic neptune Neptunea cumingi (2%), Japanese swimming crab Charybdis japonica (2%), marbled sole Pleuronectes yokohamae (1%), pen shell Atrina pectinata, ocellate spot skate Okamejei kenojei, and so on. The carapace length (CL) of each swimming crab caught in the experimental nets was measured. Sponge crab (egg-bearing female crab) was appeared from the end of June to August in the coastal waters. The master selection curve was estimated by applying the extended Kitahara's method. The selection curve showed that the gill nets of larger mesh size allowed more crabs of small carapace size to escape. The optimum value (CL/m) was 0.645 and the CL/m was estimated to be 0.301, 0.354, 0.391, 0.422, and 0.450 when the retention probabilities were 0.1, 0.2, 0.3, 0.4 and 0.5, respectively.

• Journal of Aquaculture
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• v.9 no.4
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• pp.445-452
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• 1996

Endogeneous activities of ${\beta}-galactosidase$-like enzyme in various tissues from several finfishes and shellfishes were examined by histochemical analysis based on X-gal staining and by fluorimetric measurement using 4-methylumbelliferyl-${\beta}$-D-galactoside (4-MUG). Species used in this study were 3 freshwater fishes, mud loach (Misgurnus mizolepis), common carp (Cyprinus carpio) and tilapia (Oreochromis niloticus) ; 3 marine fishes, olive flounder (Paralichthys olivaceus), stone flounder (Kareius bicoloratus) and marbled sole (Limanda yokohamae) ; and 4 shellfishes, abalone (Haliotis discus hannai), Pacific oyster (Crassoskra gigas), pearl oyster (Pinctada fucata martensii) and ark shell (Anadara broughtonii). The activities of ${\beta}-galactosidase$-like enzyme in all finfishes examined were significantly different among species, with the wide variations between tissues in a species. In general, the tissues such as kidney, intestine and liver were ones which showed the significantly higher values in 4-MUG fluorimetry and deeper staining patterns in X-gal analysis compared to other tissues. On the other hand, serum and muscle revealed the significantly lower activities than others did, regardless of species. Shellfishes were also found to have endogenous activities of ${\beta}-galactosidase$-like enzyme which were significantly varied depending on both species and organs in a species. Hepatopancreas from all shellfishes examined showed the deepest pattern in X-gal staining and also the highest value in 4-MUG analysis, while activities of ${\beta}-galactosidase$-like enzyme in adductor muscles and mantle muscles from all shellfish species in this study except Pacific oyster were negligible : Pacific oyster had the significant activity of this enzyme in muscle tissues. Putative endogenous lacZ fragment was amplified from both finfishes and shellfishes by polymerase chain reaction (PCR). The molecular size of PCR products was about 510 bp, and there was no difference in size among species examined.