• 감성과학
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• 제11권4호
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• pp.565-574
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• 2008

감성을 비롯한 인간의 뇌 기능은 혈-뇌 장벽을 매개로 약물의 작용에 의해 직접적인 영향을 받을 수 있다. 이 연구는 mannitol의 투여경로와 양, 농도에 의한 혈-뇌 장벽(blood-brain barrier, BBB)의 변화를 알아보고자 수행되었다. 실험동물로서 흰쥐에 20%의 mannitol을 오른쪽 뇌경동맥(internal carotid artery, ICA)을 통하여 주입하였고, 다른 그룹에서는 femoral vein을 통하여 주입하였다. 또한 각기 다른 경로를 이용하여 Evans blue(EB) 염색 시료를 투여한 후 BBB의 변성 정도를 확인하였다. 실험결과 ICA를 통해서 mannitol이 주입된 동물은 동측(ipsilateral side)이 대측(contralateral side)에 비해 EB 염색시료에 의해 영향을 많이 받았으나, femoral vein을 통해서 주입한 경우에는 mannitol의 양과 농도를 증가시켜도 EB 염색시료에 의한 영향이 거의 나타나지 않았다. 이러한 결과는 비록 EB 염색시료의 주입이 ICA를 통해서 또는 정맥경로를 따라서 이루어지더라도, BBB의 변성은 ICA를 통해서 이루어진다고 볼 수 있으며, 이러한 결과는 BBB의 제한적인 조절작용을 통해 인간의 뇌 기능을 이해하는데 좋은 방법을 제공할 수 있다는 것을 시사한다.

• 약학회지
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• 제1권1호
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• pp.2-4
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• 1948

Syringa oblata var dilatata (Nakai) Rehder is a oleaceous, deciduous shrub indigenous in the calcareous zones of Korea. The author researched the constituents of the leaves of the plant, especially the bitter principles which may be used as amara. According to the literature a bitter principle Syringin and Mannitol were isolated from the leaves of S. vulgaris L. And later on B. Power indicated that syringin and mannitol were widely distributed in oleaceous plants. The fresh leaves of the plant wer extracted with hot water, the filtered clear liquid was mixed with solution of lead acetate. The lead precipitate was filtered off and the filtered liquid was freed from lead by H$_{4}$S. The filtrate thus obtained was evaporated, and from the residue colorless needles, M. P. 166.deg., were obtained. It was soluble in water, in hot alcohol, and insoluble in ether and had a sweet taste. The results of the elementar analysis, M. P. and other characteristics agreed with that of d-mannitol. Finally it was proved to be identical with d-mannitol through conversion of it into Hexaacetyl mannitol, M. P. 124.deg., Triformal mannitol, M. P. 227.deg., Tribenzal mannitol, M. P. 224.deg., which exhibited no depression, when mixed with authentic specimens. The experiments to isolate bitter principles of the plant are in progress.

• BMB Reports
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• 제48권9호
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• pp.507-512
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• 2015

In the present study, we addressed the question of whether treatment with mannitol, an osmotic diuretic, affects astrogliovascular responses to status epilepticus (SE). In saline-treated animals, astrocytes exhibited reactive astrogliosis in the CA1-3 regions 2-4 days after SE. In the mannitol-treated animals, a large astroglial empty zone was observed in the CA1 region 2 days after SE. This astroglial loss was unrelated to vasogenic edema formation. There was no difference in SE-induced neuronal loss between saline- and mannitol-treated animals. Furthermore, mannitol treatment did not affect astroglial loss and vasogenic edema formation in the dentate gyrus and the piriform cortex. These findings suggest that mannitol treatment induces selective astroglial loss in the CA1 region independent of vasogenic edema formation following SE. These findings support the hypothesis that the susceptibility of astrocytes to SE is most likely due to the distinctive heterogeneity of astrocytes independent of hemodynamics. [BMB Reports 2015; 48(9): 507-512]

• Journal of Microbiology and Biotechnology
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• 제21권9호
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• pp.914-920
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• 2011

Vibrio cholerae utilizes mannitol through an operon of the phosphoenolpyruvate-dependent phosphotransferase (PTS) type. A gene, mtlD, encoding mannitol-1-phosphate dehydrogenase was identified within the 3.9 kb mannitol operon of V. cholerae. The mtlD gene was cloned from V. cholerae O395, and the recombinant enzyme was functionally expressed in E. coli as a $6{\times}$His-tagged protein and purified to homogeneity. The recombinant protein is a monomer with a molecular mass of 42.35 kDa. The purified recombinant MtlD reduced fructose 6-phosphate (F6P) using NADH as a cofactor with a $K_m$ of $1.54{\pm}0.1$ mM and $V_{max}$ of $320.8{\pm}7.81\;{\mu}mol$/min/mg protein. The pH and temperature optima for F6P reduction were determined to be 7.5 and $37^{\circ}C$, respectively. Using quantitative real-time PCR analysis, mtlD was found to be constitutively expressed in V. cholerae, but the expression was up-regulated when grown in the presence of mannitol. The MtlD expression levels were not significantly different between V. cholerae O1 and non-O1 strains.

• Journal of Yeungnam Medical Science
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• 제11권1호
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• pp.167-180
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• 1994

좌우 경동맥을 결찰하여 혈뇌장벽에 변화를 일으킨 백토에서 뇌압하강제(mannitol, steroid) 및 뇌압하강 방법(과호흡)의 효과를 관찰하였다. Mannitol군에서는 steroid군, 과호흡군에 비해 뇌압감소가 통계적으로 유의하게 있었으며, 시간적으로 mannitol 투여 후 25~30분까지 뇌압이 45%까지 감소하여 지속되었다. Steroid군에서는 mannitol군보다는 뇌압 절대치의 감소폭은 적었지만, 과호흡군 보다 2배 이상의 뇌압감소가 있었고, mannitol군보다 더욱 오랫동안 뇌압감소의 효과가 지속되었다. 과호흡군에서는 mannitol군, steroid군에서보다는 뇌압감소 효과가 적었으나, 과호흡 시행 후 10분까지 13.5~16.7%의 뇌압감소가 있었고, 그 이후 시간 경과에 따른 더 이상의 뇌압감소 효과는 없었다. Mannitol, steroid, 과호흡군을 모두 시행한 복합치료군에서는 mannitol 및 steroid, 과호흡을 각각 시행한 군에서보다 뇌압 감소가 더욱 현저하게 이루어져 30분까지 48~52%의 뇌압감소가 있었다. 이상의 결과로 미루어보아 뇌허혈성 뇌졸중 환자에서 mannitol 및 steroid사용과 병행하여 과호흡을 실시함으로써 뇌압감소를 위한 적절한 치료의 지표가 될 것이라 판단다.

• Archives of Plastic Surgery
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• 제40권6호
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• pp.721-727
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• 2013

Background One of the main concerns in orbital blowout fracture repair is a narrow operation field, due mainly to the innate complex three dimensions of the orbit; however, a deep location and extensive area of the fracture and soft tissue edema can also cause concern. Swelling of the orbital contents progresses as the operation continues. Mannitol has been used empirically in glaucoma, cerebral hemorrhage, and orbital compartment syndrome for decompression. The authors adopted mannitol for the control of intraorbital edema and pressure in orbital blowout fracture repair. Methods This prospective study included 108 consecutive patients who were treated for a pure blowout fracture from January 2007 to October 2012. For group I, mannitol was administered during the operation. Under general anesthesia, all patients underwent surgery by open reduction and insertion of an absorbable mesh implant. The authors compared postoperative complications, the reoperation rate, operation time, and surgical field improvement between the two groups. Results In patients who received intraoperative administration of mannitol, the reoperation rate and operation time were decreased; however, the difference was not statistically significant. The total postoperative complication rates did not differ. Panel assessment for the intraoperative surgical field video recordings showed significantly improved vision in group I. Conclusions For six years, mannitol proved itself an effective, reliable, and safe adjunctive drug in the repair of orbital blowout fractures. With its rapid onset and short duration of action, mannitol could be one of the best methods for obtaining a wider surgical field in blowout fracture defects.

• Journal of Korean Neurosurgical Society
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• 제60권3호
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• pp.355-361
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• 2017

Objective : The aim of this study to investigate the normal values of erythropoietin (EPO) and neuroprotective effects of quercetin and mannitol on EPO and hematocrit levels after acute severe traumatic brain injury (TBI) in rat model. Methods : A weight-drop impact acceleration model of TBI was used on 40 male Wistar rats. The animals were divided into sham (group I), TBI (group II), TBI+quercetin (50 mg/kg intravenously) (group III), and TBI+mannitol (1 mg/kg intravenously) (group IV) groups. The malondialdehyde, glutathione peroxidase, catalase, EPO, and hematocrit levels were measured 1 and 4 hour after injury. Two-way repeated measures analysis of variance and Tukey's test were used for statistical analysis. Results : The malondialdehyde levels decreased significantly after administration of quercetin and mannitol compared with those in group II. Catalase and glutathione peroxidase levels increased significantly in groups III and IV. Serum EPO levels decreased significantly after mannitol but not after quercetin administration. Serum hematocrit levels did not change significantly after quercetin and mannitol administration 1 hour after trauma. However, mannitol administration decreased serum hematocrit levels significantly after 4 hour. Conclusion : This study suggests that quercetin may be a good alternative treatment for TBI, as it did not decrease the EPO levels.

• 한국식품과학회지
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• 제20권4호
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• pp.459-462
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• 1988

국내에서 널리 인공재배되고 있는 식용버섯인 느타리버섯(Pleurotus ostreatus), 표고버섯(Lentinus edodes) 및 양송이(Agaricus bisporus)의 유리당 및 당알코올을 GCL 법으로 분석하여 버섯의 종류. 부유 및 조기별 차이점을 비교 정토한 결과는 다음과 같다. 3종의 버섯에서 trehalose, glucose, fructose, mannitol, arabitol 및 glycerol등이 확인되었다. 유리당 및 당알코올의 충 함량은 표고버섯 $12.7{\sim}18.9%$, 양송이 $9.2{\sim}16.1%$, 느타리버섯 $8.2{\sim}10.6%$ 순 이었고 표고버섯에서는 mannitol과 arabitol, 양송이에서는 mannitol, 느타리버섯에서는 tehalose와 mannitol이 특징적으로 많이 함유되어 있었다. 부위별로 비교했을때 느타리버섯과 양송이는 자루보다 갓부위에 많았으나 표고버섯은 자루부위에 많았고 크기별로는 크기가 클수록 대체로 유리당 및 당알코올 함량이 감소하는 경향이었다.

• Archives of Pharmacal Research
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• 제12권4호
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• pp.233-235
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• 1989

Sodium salts of phosphated capric and myristic acyl derivatives of mannitol were prepared and evaluated for surface activity, foam characteristics and emulsifying properties. Triacyl mannitols of cappric and myristic acid have better emulsifying property than the corresponding di and monocompounds.

• 대한한방내과학회지
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• 제21권4호
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• pp.667-670
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• 2000

The patient who has DM, Hypertension, Arrithemia, Arteral fibrillation, Heart failure attacted cerebral infartion and he was took treatment by chungpesagan-tang and mannitol at the same time for 4 days and only chungpesagan-tang for 3 days. General condition improved but at seventh day cold sweating and hypothermia appeared. So we take chest x-ray. The result is fleural effusion. Chungpesagan-tang is the method of Purgative action so heart function is injured in circulation. In the results we consider that the medication of chungpesagan-tang and mannitol at the same time is bad influence on heart and induce ftueral effusion.