• BMB Reports
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• 제39권5호
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• pp.546-555
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• 2006

An acidic polysaccharide fraction that had high anti-complementary activity was isolated from the stems of Grey Mangrove in 0.15% yield. The final fractions was designated HAM-3-IIb-II. The polysaccharide fraction appeared to be homogenous by high performance size exclusion chromatography with an estimated molecular weight of 105 kDa. The isolated polysaccharide is more effective than polysaccharide K (PSK) in its anti-complementary activity at 58 ${\mu}g/ml$ of PSK and 23 ${\mu}g/ml$ of HAM-3-IIb-II that inhibit 50% of complement activity in the complement fixation assay. Structural studies indicated that HAM-3-IIb-II was rich in galacturonic acid along with arabinose, galactose and rhamnose, characterizing a pectin-type polysaccharide, which was also confirmed by FT-IR spectrum. The presence of rich neutral sugar side chains of arabinogalactans may have contributed to the expression of high activity. Traditionally, this mangrove plant is used for medicinal purposes and it appears to have some scientific applications.

• Journal of Microbiology and Biotechnology
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• 제32권2호
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• pp.248-255
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• 2022

Phlebia sp. MG-60 is the salt-tolerant, white-rot fungus which was isolated from a mangrove forest. This fungus expresses three kinds of manganese peroxidase (MGMnP) isozymes, MGMnP1, MGMnP2 and MGMnP3 in low nitrogen medium (LNM) or LNM containing NaCl. To date, there have been no reports on the biochemical salt-tolerance of these MnP isozymes due to the difficulty of purification. In present study, we established forced expression transformants of these three types of MnP isozymes. In addition, the fact that this fungus hardly produces native MnP in a high-nitrogen medium (HNM) was used to perform isozyme-selective expression and simple purification in HNM. The resulting MGMnPs showed high tolerance for NaCl compared with the MnP of Phanerochaete chrysosporium. It was worth noting that high concentration of NaCl (over 200 mM to 1200 mM) can enhance the activity of MGMnP1. Additionally, MGMnP1 showed relatively high thermo tolerance compared with other isozymes. MGMnPs may have evolved to adapt to chloride-rich environments, mangrove forest.

• Journal of Forest and Environmental Science
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• 제26권3호
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• pp.171-179
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• 2010

Effect of chromium (Cr) stress on antioxidant enzyme activities and malondialdehyde (MDA) content were investigated in leaves and roots of mangrove (italic (L.) Druce) seedlings. Cr toxicity effects were also assessed on young seedlings. The seedlings were grown in green house condition for three months in nutrient solution with 0, 0.5, 1, 1.5, 2, 2.5, and 3 mg $L^{-1}$ $CrCl_3$. This study showed that Cr led to the change of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and activities at different concentrations. The activity of antioxidant enzymes in leaves of K. candel seedlings indicates that enzymes engaged in antioxidant defense in certain level especially in low concentration of Cr treatments. The activities of SOD and POD were activated by Cr in the root level, while CAT activity was inhibited. CAT activity decreased in response to high concentrations of Cr. In the present study indicated that SOD in root was active in scavenging the superoxide produced by Cr. Both in roots and leaves, an increase in malondialdehyde (MDA) content was observed with increase in metal concentration and exposure periods. Our finding indicated that the high concentration of excessive Cr supply may interfere with several metabolic processes of seedlings, causing toxicity to plants as exhibited by chlorosis, necrosis, photosynthetic impairing and finally, plant death.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2022년도 학술발표회
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• pp.85-85
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• 2022

The world's most extensive and active deltas, Louisiana's wetlands, are deteriorating rapidly due to multiple stressors such as the discharge of the Mississippi River, sea-level rise, and coastal retreat, the substantial but spatially and temporally variable impacts. However, the ecological and anthropogenic histories, the mode of environmental changes on a multi-millennial timescale have not been thoroughly documented. This study, a palynology-based multiproxy analysis, investigates hydrological, geological, geochemical, and anthropogenic impacts on southern Louisiana wetlands and a variety of external forcing agents influencing ecological succession. Sediment cores extracted from a small pond on a mangrove-dominate island near Port Fourchon, Louisiana, USA yielded a 4,000-year record. The site has been transformed from freshwater to saline water environments, to a mangrove dominant island over the late Holocene. The multivariate principal component analysis identified the relative strength of external drivers responsible for each ecological shift. The Mississippi River delta cycle (lobe switching) was the dominant driver of ecosystem changes during the late Holocene, while relative sea-level rise, tropical cyclones, climate, and anthropogenic effects have been the main drivers late in the site's history.

• 한국산학기술학회논문지
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• 제14권7호
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• pp.3589-3595
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• 2013

Cu, Ni 및 Cd에 노출된 망그로브 식물의 페놀성 화합물, 항산화 활성 및 지방산 함량의 변화를 분석하였다. Rhizophora mangle의 태생종자를 Cu(0.01 및 1 ppm), Ni(0.1 및 10 ppm) 및 Cd(0.1 및 10 ppm) 스트레스 하에서 12주간 배양하였다. 대조구와 비교하여 볼 때, 12주 동안 망그로브 뿌리의 형태학적 변화는 관찰되지 않았다. 12주간 배양된 태생종자에서 페놀성 화합물의 함량은 통계학적으로 유의하게 변화하지 않았으나, 항산화 활성은 크게 증가하였다. 지방산, C14:1, C15:1 및 C18:2n-6, 함량은 시험된 모든 태생종자에서 변화하였다. 이러한 결과는 DPPH 라디칼 소거능 assay와 지방산 함량분석이 중금속 하에서의 망그로브 식물의 반응을 진단할 수 있는 유용한 바이오마커가 될 수 있음을 보여준다.

• 환경영향평가
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• 제26권5호
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• pp.376-384
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• 2017

방글라데시는 인도히말라야와 인도차이나 경계에 위치한 아열대 몬순기후지역이며 생물다양성 핫스팟으로 알려져 있다. 식생은 활엽수림, 침엽수 및 혼효림으로 구성되어 있으며 습지와 망그로브로 이루어져 있다. 자연경관은 많은 강과 해안선 및 언덕과 농업 산림으로 이루어진 수려한 경관이다. 전 세계적인 선라반이라 불리는 망그로브가 있으며, 이 지역은 방글라데시와 인도 중간에 위치하고 있으며, 1999년 유네스코 세계자연유산지역이다. 망그로브의 62%가 방글라데시에 위치하고 있으며, 많은 동식물이 존재한다. 그러나 많은 사람으로 인해 자연환경이 변화고 있으며, 벌채림이 증가하고, 자연서식자가 감소된다. 이런 이유로 많은 종이 멸종에 처하게 되고 사라진 종도 많다. 이 지역 사람들은 직간접적으로 자연자원에 이익을 얻게 되며, 현재 생태관광이 주 수입원이다. 자연자원의 보호를 위해 정부는 각종 법령을 제정하고 있으며 많은 NGO가 생물다양성과 생태계 보전을 위해 노력하고 있다. 그럼에도 불구하고 현재 접경지역을 통한 오염물질과 자연재해는 방글라데시의 큰 위협요인이다.

• 환경생물
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• 제41권4호
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• pp.439-446
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• 2023

맹그로브는 육지와 바다 사이 조간대에 서식하는 식물집단으로, 전지구 탄소 순환에서 중요한 역할을 한다. 그러나 국내에 자생하는 맹그로브 관련 수종의 탄소 순환에 관한 연구는 진행된 바가 없다. 본 연구는 준맹그로브인 황근(Hibiscus hamabo) 자생지 3곳(김녕, 성산, 위미)에서 토양호흡과 잎의 기체 교환을 주기적으로 측정하고 이에 영향을 미치는 요인을 파악하고자 하였다. 토양호흡은 2022년 6월부터 10월까지, 잎의 기체 교환은 6월부터 9월까지 각 월1회씩 측정하였다. 8월의 토양호흡(5.7±0.8 μmol CO₂ m^-2 s^-1)은 다른 달에 비해 유의하게 높았으며(p<0.001), 기온이 증가함에 따라 토양호흡이 증가하였다(p<0.01). 7월에 성산에서의 순광합성률은 9.0±0.9 μmol m^-2 s^-1로 다른 시기보다 높게 나타났으며(p<0.001), 전체 기간 동안 모든 대상지에서 순광합성률은 기공전도도와 증산속도가 증가할수록 증가하였다(p<0.001). 토양호흡은 순광합성률과 양의 선형관계(r²=0.12; p<0.01)를 나타내었다. 이러한 결과는 토양호흡이 계절을 동반한 기온뿐만 아니라 광합성에 의한 CO₂ 흡수에 영향을 받기 때문인 것으로 보인다. 본 연구는 향후 준맹그로브 수종의 탄소 동태 분석에 기초 자료로 활용될 수 있을 것이다.

• Preventive Nutrition and Food Science
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• 제22권3호
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• pp.157-165
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• 2017

Fruits of Sonneratia apetala (Buch.-Ham.), (English: mangrove apple, Bengali: keora) both seeds and pericarps, are largely consumed as food besides their enormous medicinal application. The fruit seeds have high content of nutrients and bioactive components. The seeds powder of S. apetala was successively fractionated using n-hexane, diethyl ether, chloroform, ethyl acetate, and methanol. The fractions were used to evaluate antibacterial, anti-diarrhoeal, analgesic, and cytotoxic activities. Methanol fraction of seeds (MeS) stronly inhibited Escherichia coli strains, Salmonella Paratyphi A, Salmonella Typhi, Shigella dysenteriae, and Staphylococcus aureus except Vibrio cholerae at $500{\mu}g/disc$. All the fractions strongly inhibited castor oil induced diarrhoeal episodes and onset time in mice at 500 mg extract/kg body weight (P<0.001). At the same concentration, MeS had the strongest inhibitory activity on diarrhoeal episodes, whereas the n-hexane fraction (HS) significantly (P<0.05) prolonged diarrhoeal onset time as compared to positive control. Similarly, HS (P<0.005) inhibited acetic acid induced writhing in mice at 500 mg extract/kg, more than any other fraction. HS and diethyl ether fractions of seed strongly increased reaction time of mice in hot plate test at 500 mg extract/kg. All the fractions showed strong cytotoxic effects in brine shrimp lethality tests. Gas chromatography-mass spectrometry analysis of HS led to the identification of 23 compounds. Linoleic acid (29.9%), palmitic acid (23.2%), ascorbyl palmitate (21.2%), and stearic acid (10.5%) were the major compounds in HS. These results suggest that seeds of S. apetala could be of great use as nutraceuticals.

• 한국해양바이오학회지
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• 제2권1호
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• pp.1-10
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• 2007

Coral reefs are among the most dynamic and various ecosystems on tropical ecosystem. They provide a large number of important ecosystem services. Despite their importance, they appear to be one of the most susceptible marine ecosystems. Dramatic decreasing of coral reefs has been reported from every part of the world. Indonesia contains 18% of coral reefs of world's total. Unfortunately the status of coral reefs in Indonesia is already in critical and poor condition. Coral reefs communities in Indonesia are subjected to a variety of environmental disturbance. Threats to Indonesia's coral reefs resources can be divided into two main types: anthropogenic and natural disturbances. The major anthropogenic disturbances on coral reefs in Indonesia are destructive fishing, pollution, coastal development, mining and harvesting live fish and coral, tourism. The natural disturbances such as cyclones, volcanic eruptions, earthquakes, tsunami and predator also contribute to coral reefs destruction in Indonesia. In my paper I tried to compare between natural and anthropogenic disturbances on coral reefs in Indonesia and raised these questions: (i) how the natural disturbances differ from anthropogenic area (ii) which type of disturbances has caused the greatest impact on coral reef ecosystem. My finding is that both of anthropogenic and natural disturbances give major impact on coral reefs in Indonesia. The important issue here is coral reef resilience could be disturbed by synergistic effects between various anthropogenic and natural disturbances. This phenomenon has significant conservation and management implication. The appropriate management should be conducted to protect coral reefs ecosystem in Indonesia. Mangrove management will succeed only when local people are involved and get sustainable benefits from mangrove ecosystem. Community based management and Integrated Coastal Zone Management (ICZM) are type of management that can be applied on coral reef ecosystems in Indonesia.

• 한국산림과학회지
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• 제35권1호
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• pp.19-23
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• 1977

열대산(熱帶産) 광엽수재(廣葉樹材)에는 추출물(抽出物)이 많고, 특(特)히 온수추출물(溫水抽出物)이 많다. Lignin 함유량(含有量)은 Shorea속(屬)의 2수종(樹種)(Bangkirai, Red lauan)이 온대산(溫帶産) 침엽수재(針葉樹材)정도로 높고, Rhizophara속(屬)의 Mangrove재(材)는 온대산(溫帶産) 광엽수재(廣葉樹材)정도이며, Dipterocarpus속(屬)의 Keruing재(材)는 이것들의 중간(中間)정도이다. 당조성(糖組成)은 Xylose함유량(含有量)이 Shorca 속(屬)의 그 수종(樹種)이 온대산(溫帶産) 침엽수재(針葉樹材)정도로 낮고, Mangrove재(材)는 온대산(溫帶産) 광엽수재(廣葉樹材)정도이며, Kcruing재(材)는 이것들의 중간(中間)정도다. 황산(黃酸)과 무수초산(無水酢酸)에 의한 acctyl화(化)한 alditol acetate의 전수율(全收率)은 보정(補正)하여도 100%가 되지 않는다. Acetyl화(化)한 직후(直後)이면 분석(分析)에 지장이 없지만, 4~5일(日) 방치(放置)하면 분해(分解)가 일어난다. 그리고 Pyridine과 무수초산(無水酢酸)으로 acetyl환(化)한 것도 같은 경과(結果)였다.