• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.1
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• pp.103-114
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• 1994

Pearl millet is a $C_4$ plant and summer crop originated from west Africa, and the sixth most important cereal in the world and the most widely cultivated millet in the semi-arid tropics as a major staple food crop. Its grain of higher quality protein is used to make unleavened bread chapatis and prepared as gruel, dumplings, couscous and beer. It is also used as animal feed and forage in both temperate and tropical regions because it has a capability to grow well not only in the fertile soil, but also in the poor and dry soil. Most of the current breeding procedures used in pearl millet are aimed at maximum exploitation of hybrid vigor for both grain and forage yields in Korea. Pearl millet is ideally suited for exploitation of heterosis using cytoplasmic male sterile lines as seed parent, and fertile inbred lines and open-pollinated cultivars as pollen parent. Pearl millet hybrids developed in Korea produced 3 to 7 tons of grain and 100 to 150 tons of green fodder per hectare.

• Molecules and Cells
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• v.20 no.3
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• pp.416-422
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• 2005

We previously used Southern blot analysis to detect restriction-length polymorphisms between male fertile and cytoplasmic male sterile (CMS) cytoplasms at the coxII and atp6 loci of the mtDNA of Capsicum annuum L. Two copies of atp6 were found in each male fertile and CMS pepper lines. Interestingly, one of the copies of atp6 in CMS pepper was a 3'-truncated pseudogene. The open reading frame of the coxII gene was the same in the fertile (N-) and CMS (S-) lines. However, the nucleotide sequence in the S-cytoplasm diverged from that in the N-cytoplasm 41 bp downstream of the stop codon. To develop CMS-specific sequence-characterized amplified region (SCAR) markers, inverse PCR was performed to characterize the nucleotide sequences of the 5' and 3' flanking regions of mitochondrial atp6 and coxII from the cytoplasms of male fertile (N-) and CMS (S-) pepper plants. Based on these data, two CMS-specific SCAR markers, 607 and 708 bp long, were developed to distinguish N-cytoplasm from S-cytoplasm by PCR. The CMS-specific PCR bands were verified for 20 cultivars containing either N- or S-cytoplasm. PCR amplification of CMS-specific mitochondrial nucleotide sequences will allow quick and reliable identification of the cytoplasmic types of individual plants at the seedling stage, and assessment of the purity of $F_1$ seed lots. The strategy used in this report for identifying CMS-specific markers could be adopted for many other crops where CMS is used for F1 seed production.

• Journal of agriculture & life science
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• v.52 no.6
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• pp.27-36
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• 2018

Recently, the demand for new cultivar of oriental mustard(Brassica juncea) is increased as the consumption of oriental mustard has increased dramatically in the market due to Kimchi is attracting the world's attention. However, absence of seed supply system can cause many problems including inbreeding depression due to self seed production, deterioration of the seed purity and heterogeneity of commercial seed. To establish the $F_1$ variety breeding system of oriental mustard, pure line and inbred lines were screened from inbreeding genetic resources. Male-sterile line was also selected from breeding combinations for the quality improvement of mustard. The combining ability from(Indojasai ${\times}$ Goheungdamyang) combinations and isolation line of(MS910 ${\times}$ Japan red mustard 8 ${\times}$ Ganghwa mustard 9) was highest, thus these lines were selected as parental lines. PCR(Polymerase Chain Reaction) and gene sequence analysis revealed that the genes related to CMS(orf263, orf220, and orf288) were distributed in mitochondria. The isolated lines from this study also showed good performance in yield test and farmhouse prove test.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.5
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• pp.418-424
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• 1993

In order to test combining ability of the cytoplasmic-genetic male sterile(CGMS) and restorer lines with backgrounds of Korean japonica rice varieties, diallel crosses were made among the early maturing CGMS lines Sobaegbyeo A, Odaebyeo A, Gwanagbyeo A and Daeseongbyeo A, and the restorer lines Sobaegbyeo R, Odaebyeo R, Gwanagbyeo Rand Daeseongbyeo R, and among the medium maturing CGMS lines Hwajinbyeo A, Paldal A, Suwon 224 A and Iri 386 A and the restorer lines Hwajinbyeo R, Paldal R, Suwon 224 Rand Iri 386 R, and among the late maturing CGMS lines Nagdongbyeo A, Palkweng A, Hwacheongbyeo A and Milyang 97 A and the restorer lines Nagdongbyeo R, Palkweng R, H wacheongbyeo Rand Milyang 97 R. The fourty eight combinations of the hybrids, their parents and the maintainers were grown in field condition in 1992. General combining ability, relative specific combining ability and relative combining ability for yield of the CGMS and restorer lines were tested. Combining ability of the CGMS lines Odaebyeo A, Iri 386 A, Nagdongbyeo A and Palkweng A, and of the restorer lines Odaebyeo R, Hwajinbyeo R, Hwacheongbyeo Rand Palkweng R was relatively high. Heterobeltiosis for grain yield over the better parent of the early maturing hybrids ranged from -17 % to 15%, and that of the medium maturing ones from -4% to 22%, and that of the late maturing ones from -46% to 30% respectively, Standard heterosis over the standard variety of the early maturing hybrids ranged from -13 % to 12 %, and that of the medium maturing ones from 0 % to 26%, and that of the late maturing ones from -38% to 26% respectively, Positive and negative heterosis for grain yield of the hybrid rices were observed depending on cross combinations, In the positive heterosis hybrids, number of panicles per hill and number of spikelets per panicle contributed mostly to grain yield, while in the negative heterosis hybrids, spikelet fertility and 1000 grain weight were closely correlated to grain yield. Amylose content of the hybrid rices, maintainer and restorer lines was mostly lower than 20 %, and alkali digestion value was higher than 6.0. Grain appearance of the hybrids tested was similar to the korean japonica rice varieties.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.3
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• pp.227-231
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• 1984

Chinese CGMS (Cytoplasmic-Genetic Male Sterility) is being transfered to Korean cultivars which will be used as CGMS maintainers. These maintainer lines along with the Chinese CGMS lines and their maintainer lines were crossed with another group of Korean cultivars which will be used as the fertility restorers, and their yield heterosis were examined. The yield heterosis was calculated as high as 113.0-221.1 % in terms of heterosis (F$_1$/Midparent) with the grand mean of 150.5%, and, 86.1-179.8% in terms of heterobeltiosis (F$_1$/better parent) with the grand mean of 125.3%. Among the female parent groups, Chinese mainteiners were the highest following by Chinese MS lines and the Korean maintainers were the lowest. Heterobeltiosis of yield components were 92.7%, 120.0%, 103.0% and 112.0% for number of panicles/unit area, number of grains/panicle, fertility per cent and 1000 grain weight, respectively. Path-coefficients of yield components to the yield were calculated. The highest one was number of grains per panicle ranging 0.8073-0.8649 followed by the 1000 grain weight ranging 0.2000-0.5032. The grain yield ratios to the grand mean were calculated in terms of combining ability. It was highest with the Chinese maintainers followed by Chinese MS lines and The Korean maintainers were lowest. This facts bring cautions to the breeding efforts to transfer the Chinese CGMS in to Korean maintainers.

• Journal of the Korean Society of Tobacco Science
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• v.22 no.1
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• pp.39-44
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• 2000

This study was carried out to obtain the basic information about the sucker producing characteristics of certain burley tobacco varieties(Nicotiana tabacum L. var. burley) at Suwon and Chonju Experiment Station, Korea Ginseng & Tobacco Research Institute in 1999. The number and weight of suckers for 12 varieties or breeding lines were investigated three times, 45 days after transplanting for ground sucker, 10 days after topping, and 10 days after maleic hydrazide(MH) applying for upstalk sucker. KB 103 and KB 9416-1 produced less ground suckers and upstalk suckers than other entries did. KB 9210-8 produced less ground suckers and (Male sterile TC 612 x KB 108)F$_1$ also produced less upstalk suckers than other entries did. There was no differences among entries in sucker production at 10 days after MH treatment. Between the weight of total sucker and days to flower revealed the negative correlation, but not significant. The low sucker producing entries described above could be used as a parental line in the breeding program for the low sucker producing variety.

• Journal of the Korean Society of Tobacco Science
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• v.15 no.2
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• pp.111-114
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• 1993

This study was carried out to obtain the basic information for breeding of aromatic burley tobacco, and to select the breeding lines with higher DVT (diterpenes $\alpha$-and $\beta$-4, 8, 13-duvatriene-1, 3-diols) and cis-abienol (Z-labda-12, 14-diene-8$\alpha$-ol) concentrations. The parental genotypes used to develop the Fl and Fl generations were Burley 21, Ky 17, male-sterile(MS) NC 107, TC 612, TC 613, Va 509 and Tl 1068. To estimated the modes of inheritances of DVT and cis-abienol known as the flavor and aroma compounds, the method of thin - layer chromatography (TLC) was used. It was considered that TLC procedure being relatively simple and reproducible in the selection of the breeding lines with higher DVT and cis-abienol. DVT was present in all of parents except MS NC 107, but cia-abienol presented only in Tl 1068. It was estimated from the TLC analysis of parents and Fl's that DVT and cis-abienol were each inherited by dominant gene or genes. But it was less clear to determine the presence or not of sucrose ester(SE) on the TLC plate. The eleven plants which had burley leaf color and also estimated to have DVT and cis-abienol were selected in the F2 population of three combinations.

• Korean Journal of Plant Tissue Culture
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• v.24 no.4
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• pp.239-256
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• 1997

Plants belonging to the category of 2n apomixis or agamospermy form embryos and seeds without the processes of normal meiosis and syngamy. Seeds produced in this way have identical genotype of their maternal parent. Three different types of agamospermy are recognized: diplospory, apospory, and adventitious (adventive) embryony. $F_1$ hybrid cultivars cannot be used as seed sources in the next ($F_2$) generation because this generation would be extremely variable as a result of genetic segregation. Hybrid vigor is also reduced in the $F_2$ generation. Therefore, parental stocks for hybrid seed production need to be maintained and cross must be continuously repeated. Agamospermic 2n apomixis would make it possible to fix the genotype of a superior variety so that clonal seeds faithfully representing that genotype could be continuously and cheaply produced independent of pollination. That is, $F_1$ hybrid seeds could be produced for many generations without loss of vigor or genotype alteration. Production of apomictic $F_1$ hybrid seed would be simplified because line isolation would not be necessary to produce seed or to maintain parental lines, and the use of male-sterile lines could be avoided. Overall, apomixis would enable a significant reduction in hybrid seed production costs. Additionally, the production of clonal seed is not only important for seed propagated crops, but also for the propagation of heterozygous fruit trees and timbers. Clonal seed would help avoid costly and time-consuming vegetative propagating methods that are currently used to ensure the large-scale production of these plants. Apomixis is scattered throughout the plant kingdom, but few important agricultural crops possess this trait Therefore, most research to date has centered on introgressing the trait of apomixis into agricultural crops such as wheat, maize, and some forage grasses from wild distant relatives by traditional cross breeding. The classical breeding approach, however is slow and often impeded by many breeding barriers. These problems could be surmounted by taking mutagenesis or molecular approach. Arabidopsis thaliana is a tiny sexually reproducing plant and is convenient in constructing and screening in molecular researches. Male-sterile mutants of Arabidopsis are particularly suitable genetic background for mutagenesis and screening for apomictic mutants. Molecular approaches towards isolating the genes controlling the apomictic process are feasible. Direct isolation of genes conferring apomixis development would greatly facilitate the transfer of this trait to wide variety of crops. Such studies are now in progress.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.79-79
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• 2017

Grain Sorghum varieties grown in Korea have low productivity and are constrained by the fact that farmers' choice of improved varieties is limited. Hybrid cultivars have been demonstrated to be more productive and food security than pure line varieties. However, There's no available hybrid cultivars and never planted before in Korea. This study was, therefore, conducted to determine combining ability of Korean landrace varieties and cultivar, the level of heterosis of experimental hybrids depending on environments. Two cytoplasmic male-sterile lines were crossed with six male-fertile lines in accordance with North Carolina II mating scheme to generate 12 experimental hybrids. The hybrids were evaluated in replicated row-column alpha designs at two sites in Texas and South Korea under on-season production conditions. For each trait, general combining ability (GCA) and specific combining ability (SCA) effects were estimated using the line-tester method of analysis. Results indicated significant differences among genotypes for both grain yield potential and secondary traits. Hybrids were predominant for grain yield, and displayed up to 127% heterosis of A03017 ${\times}$ Sodamchal in Korea. Positive GCA for yield was observed for Donganme, Hwanggeumchal and Jungmo4002 in Korea. Among them Jungmo4002 and Donganme had highly positive for yield (227.90 and 84.90 kg ha-1), while it showed negative GCA for yield in College station (-40.90, -189.60 kg ha-1). ATx630 ${\times}$ Sodamchal had the greatest SCA for yield (468.10 kg/ha) in College station but at South Korea its SCA effect was -302.40 kg/ha. A similar trend was observed, followed by A03017 ${\times}$ Donganme (SCA effect = 215 kg ha-1 at College station vs. -44.20 kg ha-1 at South Korea). At South Korea the greatest positive SCA effect for grain was observed in ATx630 ${\times}$ Jungmo4002 as 322.20 kg/ha. Both GCA and SCA effects significantly interacted with site effects demonstrating the need for region adaptation of potential cultivars and test for developing Korea suitable cultivar.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.4
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• pp.431-435
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• 1985

Nine hybrid rices crossed between Korean cytoplasmic-genetic male sterile rices having the WA cytoplasm and the Korean restorer lines or varieties, HR1619A/Nampungbyeo, HR1619A/Gayabyeo, HR1619A/Line234, TongilA/Nampungbyeo, TongilA/Cheongcheongbyeo, Suwon 296A/Line 209, Suwon 296A/Line 237, Suwon 296A/Line 252 and Line 201A/Line 234, and their parents were grown at Yeungnam University in 1984. The rough rice yield of the hybrids Line 201A/Line 234, TongilA/Nampungbyeo and HR1619A/Nampungbyeo were 939, 927 and 900 Kg/10a respectively. The heterosis(F$_1$/Midparent) of the above three hybrids was 40%, 20% and 19%, the heterobeltiosis(F$_1$/Better parent) was 36%, 17% and 10%, and the standard heterosis (F$_1$/Standard variety, Cheongcheongbyeo) was 19%, 17% and 14% respectively. The hybrids HR1619A/ Gayabyeo and Suwon 296A/Line 237 showed negative heterosis in grain yield. Significant heterobeltiosis for grain number per panicle was found while less or no heterobeltiosis was observed in panicle number per hill, 1000-grain weight and grain fertility. The bacterial leaf blight disease reaction of the hybrids tested was almost the same as that of one parent at least. The amylose content of the hybrids was medium to low the same as their parents. The protein content and alkali digestion value of the hybrids were almost the same as their parents.