농업생명과학연구 (Journal of agriculture & life science)

  • 제52권6호
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  • Pages.27-36
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  • 2018
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  • 1598-5504(pISSN)
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  • 2383-8272(eISSN)
경상대학교 농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)
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웅성불임 인자를 이용한 갓(Brassica juncea L. Czern)의 F1 육종

Breeding of F1 Hybrid for Oriental Mustard(Brassica juncea L. Czern) Using the Cytoplasmic Male Sterile Line

  • 박용주 (경북대학교 생태환경대학 생태환경시스템학부) ;
  • 민병환 (경북대학교 생태환경대학 생태환경시스템학부)
  • Park, Yong Ju (Division of Ecological & Environmental System, Kyungpook National University) ;
  • Min, Byung Whan (Division of Ecological & Environmental System, Kyungpook National University)
  • 투고 : 2018.09.14
  • 심사 : 2018.10.16
  • 발행 : 2018.12.31
⟨ 이전 논문 다음 논문 ⟩

초록

김치가 세계 5대 건강식품으로 선정되어 세계인의 주목을 받게 되면서 갓의 수요도 점점 증가하고 있어 다양한 갓품종의 육성이 절실히 요구되는 실정이다. 그러나 아직 품종보급 체계가 미흡하여 자가 채종에 의한 자식약세현상, 순도저하, 상품의 불균일 등의 문제점이 발생하고 있다. 본 실험에서는 갓의 F1 품종 육성체제를 갖추기 위하여 유전자원으로 수집한 갓의 순계분리와 자식계통의 육성, 웅성불임인자의 도입을 통하여 고품질의 갓을 육성하기 위하여 각 조합을 공시하여 조합능력을 검정하고(인도자사이${\times}$고흥담양)조합과 (분리 MS 유기계-MS910${\times}$일본 적자색갓 8${\times}$강화갓 9) 분리계 조합이 우수하다고 판단되어 양친으로 선발하였다. PCR(Polymerase Chain Reaction)과 염기서열 분석을 통해 CMS type을 확인해 본 결과, MS계통 MSLS와 MSLC는 갓에서 보고된 orf263, orf220 및 orf288 CMS 유전자를 미토콘드리아 DNA에 포함하고 있는 것으로 나타났다. 최종적으로 생산력검정 및 농가 실증시험을 실시하였다.

Recently, the demand for new cultivar of oriental mustard(Brassica juncea) is increased as the consumption of oriental mustard has increased dramatically in the market due to Kimchi is attracting the world's attention. However, absence of seed supply system can cause many problems including inbreeding depression due to self seed production, deterioration of the seed purity and heterogeneity of commercial seed. To establish the $F_1$ variety breeding system of oriental mustard, pure line and inbred lines were screened from inbreeding genetic resources. Male-sterile line was also selected from breeding combinations for the quality improvement of mustard. The combining ability from(Indojasai ${\times}$ Goheungdamyang) combinations and isolation line of(MS910 ${\times}$ Japan red mustard 8 ${\times}$ Ganghwa mustard 9) was highest, thus these lines were selected as parental lines. PCR(Polymerase Chain Reaction) and gene sequence analysis revealed that the genes related to CMS(orf263, orf220, and orf288) were distributed in mitochondria. The isolated lines from this study also showed good performance in yield test and farmhouse prove test.

키워드

참고문헌

  1. Adey SS, Sharma SR, Bhatia R, Parkash C and Barwal RN. 2011. Superior CMS(Ogura) lines with better combining ability improve yield and maturity in cauliflower(Brassica oleracea var. botrytis). Euphytica. 182: 187. https://doi.org/10.1007/s10681-011-0425-y
  2. Farrell KT. 1985. Spices, Condiments and Seasonings, 150-155. Van Nostant Company, New York.
  3. Heng S, Wei C, Jing B, Wan Z, Wen J, Yi B, Ma C, Tu J, Fu T and Shen J. 2014. Comparative analysis of mitochondrial genomesbetween the hau cytoplasmic male sterility(CMS) line and its isonuclear maintainer line in Brassicajuncea to reveal the origin of the CMS-associated gene orf 288. BMC Genomics. 15: 322-334. https://doi.org/10.1186/1471-2164-15-322
  4. Hirata Y, Motegi T, Takeda Y and Morikawa K. 2000. Inducion of cytoplasmic male sterility in the seed progeny derived from artificially-synthesized interspecific chimera in Brassica. Euphytica. 117: 143-149.
  5. Jing B, Heng S, Tong D, Wan Z, Fu T, Tu J, Ma C, Yi B, Wen J and Shen J. 2012. A male sterility associated cytotoxic protein ORF288 in Brassica juncea causes aborted pollen development. J. of Experimental Botany. 63: 1285-1295. https://doi.org/10.1093/jxb/err355
  6. Kumar P, Vasupalli N, Srinivasan R and Bhat SR. 2012. An evolutionarily conserved mitocondrial orf 108 is associated with CMS in different alloplasmic lines of Brassica juncea and induces male sterility in transgenic Arabidopsis thaliana. J. of Experimental Botany. 63: 2921-2932. https://doi.org/10.1093/jxb/err459
  7. Lee IH, Park JI, Jung GH and Nou IS. 2010. Variation of characteristics and photosynthetic rates among the species of leaf mustard(Brassica juncea). J. of Life Science. 20: 1127-1133a. https://doi.org/10.5352/JLS.2010.20.7.1127
  8. Lee IH, Park JI, Jeong OS, Jung HJ, Jung GH and Nou IS. 2010. Genetic Relationship Based on RAPD Analysis of Yeosu Dolsan Leaf Mustard (Brassica juncea). J. of Life Science. 20: 66-70b. https://doi.org/10.5352/JLS.2010.20.1.066
  9. Nahm SH, Lee HJ, Joo GY, Harn CH, Yang SG and Min BW. 2005. Development of a molecular marker specific to a novel CMS line in radish (Raphanus sativus L.). Theor. Appl. Genet. 111: 1191-1200. https://doi.org/10.1007/s00122-005-0052-x
  10. Ohkawa Y. 1986. Comparison of B. napus to B. campestris and B. oleracea based on the cytoplasmic characters cytoplasmic male sterility and chloroplast DNA. JARQ. 19: 244-257.
  11. Okazaki K and Hinata K. 1987. Repressing the expression of self-incompatibility by short term high temperature treatment. Theor. Appl. Genet. 73: 496-500. https://doi.org/10.1007/BF00289185
  12. Yang J, Liu X, Xu B, Zhao N, Yang X and Zhang M. 2013. Identification of miRNAs and their targets using high-throughput sequencing and degradome analysis in cytoplasmic male-sterile and its maintainer fertile lines of Brassica juncea. BMC Genomics. 14: 9-24. https://doi.org/10.1186/1471-2164-14-9
  13. Yang X, Liu X, Lv W, Li L, Shi Q, Yang J and Zhang M. 2012. Reduced expressionof BjRCE1 Gene modulated by nuclear-cytoplasmic incompatibility alters auxin response in cytoplasmic male-sterile Brassica juncea. PLoS one. 7: 1-9.
  14. Zhao N, Xu X, Wamboldt Y, Mackenzie SA, Yang X, Hu Z, Yang J and Zhang M. 2014. MutS HOMOLOG1 silencing mediates ORF220 substoichiometric shifting and causes male sterility in Brassica juncea. J. of Experimental Botany. 67: 435-444.