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Overcoming sterility by Caffeine and Temperature in Oriental-Asiatic Interspecific Lily Hybrid (백합 Oriental-Asiatic 종간잡종의 임성 회복을 위한 카페인과 변온처리)

  • Park, Song Kyoung;Park, In Sook;Kim, Chang Kil;Jee, Sun Ok;Lim, Ki Byung
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.4
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    • pp.279-284
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    • 2009
  • In order to recover fertility from sterile interspecific OA-1 $F_1$ hybrid (Oriental hybrid 'Mero Star' ${\times}$ Asiatic hybrid 'Connecticut King'), various concentrations (0.1, 0.3 and 0.5%) of caffeine were injected directly into flower buds and then confirmed the viability of OA-1 $F_1$ hybrid at the flowering time. After the caffeine treatment, fertilized $F_1$ hybrids were crossed as female with Asiatic hybrid 'Lanzarote' as male. Five plantlets were obtained from seven embryos of 16 pollinated flowers at 0.3% treatment of caffeine while 0.5% treatment of caffeine obtained one plant let and 0.1 % treatment of caffeine plantlet did not produce at all. Thus 0.3% of caffeine treatment was considered as optimum concentration to produce subsequent progenies and the OA-l $F_1$ hybrid treated with caffeine produced 51% of putative 2n gametes. Pollen germination of OA-2 ('Romero Star' ${\times}$ 'Lady Rosa') and OA-3 ('Expression' ${\times}$ 'Lady Rosa') was not differ between temperature treatment alone and in combination with caffeine and temperature treatment. In the reciprocal crosses of OA-1 and Asiatic hybrid 'Lanzarote' or Oriental hybrid 'Sorbonne', A ('Lanzarote') ${\times}$ OA-1 or OA-1 ${\times}$ A crosses showed better results than O ('Sorbonne') ${\times}$ OA-1 or OA-1 ${\times}$ a crosses in plant obtaining. All progenies obtained from A ${\times}$ OA-1 or OA-1 ${\times}$ A crosses were confirmed as triploids by GISH analysis.

A study on the level of codependency among social work related professionals and its effect on their burnout: Focusing on moderating effects of Sex-role attitude (사회복지종사자의 공동의존성과 소진에 관한 연구 : 성역할태도의 조절효과를 중심으로)

  • Jang, Gyeongho;Yoon, Kyeonga;Shim, Woochan
    • Korean Journal of Social Welfare Studies
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    • v.47 no.1
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    • pp.29-56
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    • 2016
  • The purpose of this study is first, to explore the level of codependency among social work related professionals since codependency of professionals in the field has not been explored much in Korea. Second purpose is to examine whether codependency affects the level of burnout. Last purpose is to examine moderating effects of the Sex-role attitude on the relationship between codependency and burnout. Data were collected from the 11th of December in 2014 until the 28th of February in the following year. Self-administered questionnaires were distributed to care professionals practicing in social work institutions in Daejeon City and Choongcheong province, via email and mail. In addition, surveys were administered at several continuing education training venues. Instruments used for this study include CODAT(Codependency Assessment Tool) developed by Hughes-Hammer and colleagues in 1998, MBI(Maslach Burnout Inventory) developed by Maslach and Jackson in 1981, and SRA(Sex Role Attitude) developed by Osmond and Maryin in 1975. In total, 368 surveys were used for final analysis. The results include that gender did not have statistically significant relationship with the overall codependency, however, male showed statistically higher score than female in one of subscales of codependency called hiding oneself. Workers in the residential settings showed higher level of codependency than those in non-residential settings, especially in the subscale of Other focus or Self-neglect. Codependency showed a strong relationship with Burnout. Among the five-factor structure of the CODAT, Medical problem, Low Self-worth and Family of Origin showed strong relationship with the Burnout score. And the moderating effects of Sex-role Attitude showed a statistically significant relationship between codependency and burnout. Conservative sex-role attitude amplified the level of burnout on the relationship between codependency and burnout. Based on the result, educational and policy implications were made in order to prevent burnout among social work related professionals.

The Effect of Ex-prisoner's Perception of Stigma on Trust in Interpersonal Relationship (낙인에 대한 출소자의 인식이 대인관계에서 신뢰에 미치는 영향)

  • Gong, Jung Sik
    • Korean Security Journal
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    • no.57
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    • pp.57-84
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    • 2018
  • This study assumes that the perception of the ex-prisoner's against stigma will affect trust in interpersonal relationship. Prior studies have suggested that there is a stigma at the base of the awareness where people avoid and discriminate against ex-prisoner's. This in turn affects ex-prisoner's' recividicism, but there is no study about ex-prisoner's perception of stigma in Korea. Although stigma can be explained in numerous ways, this study is based on the opinion of Phillips(2016), which divides the subscale of stigma as perceived public stigma, perceived personnel stigma, and self stigma. He insisted that ex-prisoner's believe perceived public stigma higher than self-stigma. these results are consistent with this study. However, it is important to note that although ex-prisoner's are more aware of public stigma than self-stigma, they are actually more influenced by self stigma in pereonal relationships. This means that ex-prisoner's are more susceptible to internal psychological awareness than external social recognition. Therefore, the development and intervention of the program to overcome the internal self-stigma of the released prisoner is required. In this study, first, stigma, especially self-stigma turned out to have influence on the interpersonal trust. Therefore, as self-stigma level increased, the interpersonal trust decreased in most cases. Second, it was shown that stigma has the greatest influence on children out of family members, co-workers out of social relationship, self trust out of general relationship in. This confirms that stigma is a factor that greatly influences relationship between important people for ex-prisoner's. Third, since self-sigma negatively reestablish self-identity, make individuals recognize themselves as deviators and show bad lifestyle, which lead them to become habitual offender, ex-prisoner's need to make efforts to overcome self-stigma, and development and intervention of program that can make ex-prisoner's have positive self identity is requested. Fourth, although participants in the study were only male, it seems that there is difference in recognition of stigma by gender, and influence of stigma not only on interpersonal relationships, but also on social reintegration and recidivism imply that these might be good future research topics.

Research on Classifying the 'Sijochang', or Korean Ode Narrative Song (시조창 분류고)

  • Shin Woong-Soon
    • Sijohaknonchong
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    • v.24
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    • pp.223-258
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    • 2006
  • This Research is about the classification of 'Sijochang', or the Korean ode narrative song, in terms of music. Contrary to the literature classification by the number of letters, sijochang varies with the melody. Literally, the classification is generally made as Dansijo(or short ode) Jungsijo(or medium ode) and Jangsijo(or lengthy ode) but the sijochang is normally divided into 'Pyongsijo' (or plain ode), 'Jirumsijo and Saseolsijo'. As while the same Sijochang is called under the different names, the different type of sijochang is also called as the same name, it needs the discussion about its name. Some Korean classical musicians have attempted to define it but they are trying to do it without the specific reasoning about its concept. As a result, the systematic research is required. This study designs to streamline the currently confusing and complex names and set up the sijo's classification system. After reviewing the ancient music note, current sijo score and the traditional theory, I largely classified it into 3 types: Pyongsijo, Jirumsijo and Saseolsijo. And then, 1 analyzed on to which type the sijochang which is presently called belongs, based on several principles. The 67 names of the sijo which I have investigated about are classified with them sharpy reduced into 16. Among the current sijo names. there are some which are of same type yet of different phonetics and there are others which are of different phonetics yet of same type. To avoid such complex and troublesome names, I have orchestrated them as follows, taking the literary and music concept into account. 1) Pyongsijo type : Pyongsijo, Joongherisijo, Wujosijo and Payeonkok 2) Jirumsijo type: Jirumsiro, Namchangjirumsijo(it refers to Jirumsijo sung by male ), Yeochangjirumsijo (it refers to Jirumsijo sung by female), Banjirumsijo(it refers to half the Jiumsiro), Onjirumsijo (it refers to the whole Jirumsijo), Wujojr\irumsijo, Saseoljirumsijo and Whimorisijo) 3) Saseolsijo type : Saseolsijo, Bansaseolsijo(it refers to half the Saseolsijo, Gaksijo or Pyongsiro There are still lots of things to musically streamline, in the fields of disposition of Sijo letters, its form, musical scale and influences on other genre. etc. and as such. the accumulation of theory on them is urgently required. Those musical elements need an in-depth review and study by the experts and the Korean traditional musicians. Later research is expected to play a role of exploring it.

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The Studies on the Development of Low Irritable Preservative System with Phenoxyethanol in Cosmetics (Phenoxyethanol을 이용한 저자극 방부시스템 개발에 관한 연구)

  • Ahn, Gi-Woong;Lee, Chn-Mong;Kim, Hyeong-Bae;Jeong, Ji-Hen;Jo, Byoung-Kee
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.31 no.1 s.49
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    • pp.43-49
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    • 2005
  • Recently, according as people who have sensitive skin increase, we've been giving more importance to the safety of cosmetics. Especially, preservative is known to be one of the main stimuli which cause side-effects of cosmetics. However, there have been few reports describing cell cytotoxicity, skin penetration, oil-aqueous phase partition, anti-microbial activity of preservatives and their correlation with skin irritation. The study is aimed to develop low irritable preservative system with phenoxyethanol, one of the most commonly used preservatives in cosmetics, considering various factors mentioned above. According to our results of cell cytotoxicity against human normal fibroblasts by means of MTT assay, phenoxyethanol showed the lowest cytotoxicity when compared to other preservatives tested (cytotoxicity: pro-pylparaben > butylparaben > ethylparaben > methylparaben > triclosan > phenoxyethanol), but human patch test for assessing shin primary irritation revealed that phenoxyethanol has higher skin irritation than methylparaben and triclosan. We performed in vitro skin penetration test using horizontal Franz diffusion cells with skin membrane prepared from hairless mouse (5 ${\~}$ 8 weeks, male) to evaluate the rate of skin penetration of preservatives. From the results, we found that the higher irritable property of phenoxyethanol in human skin correlates with its predominant permeability (skin penetration: phenoxyethanol > methylparaben > ethylparaben > propylparaben > butylfaraben > triclosan). Therefore, we made an effort to reduce skin permeability of phenoxyethanol and found that not only the rate of skin penetration of phenoxyethanol but also its skin irritation is dramatically reduced in formulas containing oils with low polarity. In the experiments to investigate the effect of oil polarity on the oil-aqueous phase partition of phenoxyethanol, more than $70\%$ of phenoxyethanol was partitioned in aqueous phase in formulas containing oils with low polarity, while about $70 {\~} 90\%$ of phenoxyethanol was partitioned in oil phase in formulas containing oils with high polarity. Also, in aqueous phase phenoxyethanol showed greater anti-microbial activity. Conclusively, it appears that we can develop less toxic preservative system with reduced use dosage of phenox-yethanol and its skin penetration by changing oil composition in formulas.

Regional Disability Free Life Expectancy and Related Factors in Korea (우리나라 지역별 건강수명과 관련요인)

  • Han, So-Hyun;Lee, Sung-Kook
    • Korea journal of population studies
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    • v.35 no.2
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    • pp.209-232
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    • 2012
  • The purposes of this study is to calculate the gender-based Life Expectancy and Disability Free Life Expectancy of 65-year-olds in accordance with the regions of 16 in Korea based on the years 2005 and 2010 by using the Sullivan method. We used the Census in 2005, 2010, the Korea National Statistical Office(10% sample). We also reviewed examining the changes in LE and DFLE and understanding the relevance between the DFLE of 65-year-olds of the year 2010 and related factors. As a result of this study, it was shown that the LE of the group of all 65-year-old in Korea increased from 18.15 years in 2005 to 19.75 years in 2010 and DFLE increased from 11.41 years in 2005 to 11.64 years in 2010. Regionally, the highest LE for total of 65-year-olds was found to be the entire Jeju area and DFLE was the highest in the city of Seoul. And the highest LE and the highest DFLE for 65-year-old male were found to be in the city of Seoul. Regarding the regional LE of 65-year-old female, both in 2005 and 2010 Jeju area was seen to be the highest. The results of a correlation analysis showed that the DFLE of 65-year-olds in 2010 was found to be increasing with high LE and high population density and in regions with low average temperatures, low number of beds and low age standardized death rates of malignant neoplasms and circulatory disease.

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Effect of Leuconostoc kimchii GJ2 Isolated from Kimchi (Fermented Korean Cabbage) on Lipid Metabolism in High Cholesterol-Fed Rats (김치로부터 분리한 유산균 Leuconostoc kimchii GJ2가 고콜레스테롤식이를 급여한 흰쥐의 지질대사에 미치는 영향)

  • Lee, Jae-Joon;Lee, Yu-Mi;Kim, Ah-Ra;Chang, Hae-Choon;Lee, Myung-Yul
    • Food Science and Preservation
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    • v.15 no.5
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    • pp.760-768
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    • 2008
  • The present study investigated the effect of Leuconostoc kimchii GJ2 (Leu. kimchii GJ2), an exopolysaccharide-producing lactic acid bacterium isolated from kimchi, on serum and liver lipid metabolism in rats fed a high-cholesterol diet. Male Sprague-Dawley rats were divided into four groups: a normal diet group (ND), a high-cholesterol diet group (HCD), a high-cholesterol diet and 200 mg/kg Leu. kimchii GJ2-administered group (HCD-LKL), and a high-cholesterol diet and 400 mg/kg Leu. kimchii GJ2-administered group (HCD-LKH). No between-group differences were found in body weight gain, food intake, or food efficiency ratio. The serum GOT and ALP activities that were elevated by the high-cholesterol diet were significantly decreased after Leu. kimchii GJ2 administration. Serum HDL-cholesterol level was markedly increased in the Leu. kimchii GJ2-administered groups, whereas the serum total cholesterol and LDL-cholesterol levels were lower in the Leu. kimchii GJ2-administered animals. Liver levels of total cholesterol and triglyceride were also markedly lower in the Leu. kimchii GJ2-administered groups. In addition, increased activities of HR-LPL and TE-LPL in adipose tissue, caused by the high-cholesterol diet, fell to normal after administration of Leu. kimchii GJ2, in a dose-dependant manner. These results suggest that Leu. kimchii GJ2 isolated from kimchi exerts an antiatherosclerotic effect by reducing serum and liver cholesterol levels.

Effects of Vitamin C on Residual Aflatoxin $B_1$ in Rat Sera Treated with Radiation and Aflatoxin $B_1$ (Vitamin C가 방사선과 Aflatoxin $B_1$을 투여한 흰쥐의 혈청 중 Aflatoxin $B_1$ 잔류량에 미치는 영향)

  • Chung, Do-Young;Kim, Han-Soo;Kang, Jin-Soon
    • Food Science and Preservation
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    • v.18 no.3
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    • pp.374-382
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    • 2011
  • Aflatoxin ($AFB_1$) is a potent hepatotoxic and hepatocarcinogenic mycotoxin in humans. It is also well-known to be accumulated in animal tissues via various metabolic pathways. This study was conducted to determine the effects of vitamin C on the residual $AFB_1$ in rat sera that were treated with radiation and $AFB_1$. Six week-old male Sprague-Dawley rats were randomly divided into five groups: a control group, $AFB_1$-treated group, the group treated with $AFB_1$ and vitamin C, the group treated with X-ray and AFB1, and the group treated with X-ray and $AFB_1$ with vitamin C. On the first day of the experiment, only one dose of X-rays was exposed to the entire liver at 1,500 cGy. Next, vitamin C was injected at 10 mg/kg body weight via intraperitoneal injection, followed 1 hr later by the administration of 0.4 mg/kg of $AFB_1$ via intraperitoneal injection. These treatments were then administered every three days over a period of 15 days. On the 16th day of treatments, the animals were sacrificed. The contents of $AFB_1$ in rat sera were determined via indirect competitive ELISA and HPLC method. In the quantitative analysis of $AFB_1$ in rat sera via ELISA, $5.17{\pm}0.34$ ng/mL of $AFB_1$ was detected in the $AFB_1$-treated groups, but the amount more significantly decreased to $3.23{\pm}0.76$ ng/mL in the groups treated with $AFB_1$ and vitamin C (p<0.01) than in the $AFB_1$-treated groups. The $AFB_1$ contents of the rat sera of the groups treated with X-ray and $AFB_1$ did not significantly decreased with the administration of vitamin C. The $AFB_1$ content of the rat sera that was analyzed via HPLC showed a tendency similar to that of the content that was analyzed via ELISA. With regard to these data, vitamin C was very effective in reducing $AFB_1$ residue in rat sera.

Body-Weight-Loss and Cholesterol-Lowering Effects of Cheonggukjang (a Fermented Soybean Paste) Given to Rats Fed a High-Fat/High-Cholesterol Diet (청국장이 고지방-고콜레스테롤식이를 급여한 흰쥐에서 체중감소 및 콜레스테롤 저하효과)

  • Kim, Ah-Ra;Lee, Jae-Joon;Lee, Hwan;Chang, Hae-Choon;Lee, Myung-Yul
    • Food Science and Preservation
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    • v.17 no.5
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    • pp.688-697
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    • 2010
  • We investigated the cholesterol-lowering and body-weight loss effects of Cheonggukjang fermented using Bacillus subtilis DJI in rats fed a high-fat/high-cholesterol diet for 4 weeks. Weight-matched male Sprague-Dawley rats were assigned to one of four groups: a normal diet group (N), a high-fat/high-cholesterol diet group (HFC), a high-fat/high-cholesterol diet with DJI Cheonggukjang group (HFC-SCK), and a high-fat/high-cholesterol with commercial Cheonggukjang group (HFC-CCK). All of body weight and liver and adipose tissue weights increased in animals fed a high-fat/high-cholesterol diet, but decreased significantly in rats fed Cheonggukjang powder, compared with the HFC group. Food intake was lower in the HFC group than in the N group, and that of the HFC-CCK group was the lowest among the four groups. Serum total cholesterol levels were significantly lower in the Cheonggukjang-powder fed groups than the other groups. Serum phospholipid and HDL-cholesterol concentrations were significantly decreased in HFC animals and were markedly increased upon feeding of a Cheonggukjang-containing-diet. Levels of serum LDL-cholesterol, the atherogenic index, and cardiac risk factor assessment indications tended to be decreased in Cheonggukjang powder-fed groups, compared with the HFC group. The total cholesterol level in liver tissue was increased by feeding of a high-fat/high-cholesterol diet, and was significantly reduced when Cheonggukjang powder was present in the diet. The levels of total lipids and triglycerides in adipose tissues were lower in the HFC-SCK group than in the HFC group, whereas no significant differences were evidence when the HFC and the HFC-CCK groups were compared. Fecal weight, moisture level, and total lipid content increased in animals fed Cheonggukjang powder. The activities of HR-LPL and TE-LPL in adipose tissues were increased in the HFC group compared with the Cheonggukjang powder-fed groups. These results indicate that dietary Cheonggukjang may improve lipid metabolism and prevent obesity and hyperlipidemia.

Development of Anti-diabetes Drink Using with Silkworm(Bombyx mori L.) Extract (누에(Silkworm) 추출물을 이용한 기능성 항당뇨음료의 개발)

  • 최진호;김대익;박시향;백승진;김남주;류강선
    • Journal of Sericultural and Entomological Science
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    • v.45 no.2
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    • pp.96-102
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    • 2003
  • This study was designed to develope the functional anti-diabetes drink, Dia-D using silkworm (Bombyx mori L) extract Sprague-Dawley (SD) male rats (160${\pm}$10g) were fed basic diet (control group), and experimental diets (SWE-l0, 30, 60 groups and Daonil-40, 80 groups) added silkworm extract 10, 30 and 60mg/day or diabetes drug prepared and marketed by Handok Pham. Co., Daonil 40 and 80 mg/day for 12 days. Blood glucose contents were significantly decreased 25-30% in SWE-30 and SWE-60 groups, and about 35% in Daonil 40 and Daonil 80 groups compared with control group. Triglyceride (TG) and lipid peroxide (LPO) contents were significantly inhibited 10-16% and 8-13%, respectively, in SWE-30 and 60 groups, whereas these contents were 13-30% and 15%, respectively, in Daonil-40 and 80 groups compared with control group. Hydroxyl radical (OH) contents were significantly inhibited 19-20% in SWE-30 and 60 groups, and 7-12% in Daonil-40 and 80 groups compared with control group. Superoxide dismutase (SOD) activities were significantly increased 11-14% in SWE-30 and 60 groups, and 12-29% in Daonil-40 and 80 groups compared with control group. In results of clinical test using normal subjects, blood glucose content tested in NIAST subjects as anti-diabetes drink, Dia-D willi 100mg/vial was significantly decreased 17.5%, and these content tested in PKNU subjects as anti-diabetes drink, Dia-D with 150mg/vial was significantly decreased 20.5% compared with control group. These results suggest that administration of Dia-D as an anti-diabetes drink prepared with silkworm extract may playa very effective role in a decreasing of blood glucose in hyperglycemia patients.