• Title/Summary/Keyword: mMT

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Immunogold Labellings and Expression of Metallothionein in Regenerating Rat Liver (재생중인 흰쥐 간의 메탈로사이오닌에 대한 면역-금 표지 및 발현에 관한 연구)

  • Ahn, Young-Mo;Oh, Seung-Han;Kim, Ho-Jin;Lee, Mi-Young;Lee, Jong-Hwa;Shin, Kil-Sang;Kim, Wan-Jong
    • Applied Microscopy
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    • v.35 no.1
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    • pp.15-22
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    • 2005
  • Metallothionein (MT) is a low molecular weight, cysteine-rich, metal-binding, and non-enzymatic protein. The present study was carried out to investigate the expression of MT gene as well as the localization of MT in regenerating rat liver. In partial hepatectomized rats, MT mRNA was detected as early as 1 hr and reached a maximal level by 8 hr after the operation. Thereafter, this level decreased gradually until 24 hr, and it became similar to that of sham control. Meanwhile, time course of MT immunoreactivity using immunogold-labelling revealed that the number of gold particles in hepatocytes increased significantly by 12 hr, but decreased at 48 hr after partial hepatectomy. Ultrastructurally, immunogold particles indicating the presence of MT were distributed in both the cytoplasm and the nucleus of the rat hepatocytes, particularly the particles were distributed at rough endoplasmic reticulum and nucleolus and did not seem to adhere to mitochondria or lysosomes in proliferating hepatocytes. Briefly, high level of MT mRNA expression and the intense immunoreactivity and/or the specific localization of MT was observed during liver regeneration. These results suggest that MT possibly involves hepatocyte proliferation via the storage or the supply of various metal ions in the regenerating rat liver.

CYP1A1 Gene Polymorphisms: Modulator of Genetic Damage in Coal-Tar Workers

  • Giri, Shiv Kumar;Yadav, Anita;Kumar, Anil;Dev, Kapil;Gulati, Sachin;Gupta, Ranjan;Aggarwal, Neeraj;Gautam, Sanjeev Kumar
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.7
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    • pp.3409-3416
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    • 2012
  • Aim: It is well known that polycyclic aromatic hydrocarbons (PAHs) such as benzo (a) pyrene have carcinogenic properties and may cause many types of cancers in human populations. Genetic susceptibility might be due to variation in genes encoding for carcinogen metabolizing enzymes, such as cytochrome P-450 (CYP450). Our study aimed to investigate the effect of genetic polymorphisms of CYP1A1 (m1 and m2) on genetic damage in 115 coal-tar workers exposed to PAHs at their work place. Methods: Genetic polymorphisms of CYP1A1 were determined by the PCR-RFLP method. Comet and buccal micronucleus assays were used to evaluate genetic damage among 115 coal tar workers and 105 control subjects. Results: Both CYP1A1 m1 and CYP1A1 m2 heterozygous and homozygous (wt/mt+mt/mt) variants individually as well as synergistically showed significant association (P<0.05) with genetic damage as measured by tail moment (TM) and buccal micronuclei (BMN) frequencies in control and exposed subjects. Conclusion: In our study we found significant association of CYP1A1 m1 and m2 heterozygous (wt/mt)+homozygous (mt/mt) variants with genetic damage suggesting that these polymorphisms may modulate the effects of PAH exposure in occupational settings.

Selection of 5-Methyltryptophan and S-(2-Aminoethyl)-L-Cysteine Resistant Microspore-Derived Rice Cell Lines Irradiated with Gamma Rays

  • Kim, Dong-Sub;Lee, In-Sok;Jang, Cheol-Seong;Hyun, Do-Yoon;Lee, Sang-Jae;Seo, Yong-Weon;Lee, Young-Il
    • Journal of Plant Biotechnology
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    • v.5 no.1
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    • pp.33-41
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    • 2003
  • Microspore-derived cell lines resistant to 5-methyltryptophan (5MT, a tryptophan analog) or S-(2-aminoethyl)-L-cysteine (AEC, a Iysine analog) were selected in rice by in vitro mutagenesis. For selection of 5MT or AEC resistant cell lines, suspension-cultured cells were irradiated with gamma rays. Thirteen 5MT resistant cell lines were selected and they were able to grow stably at 2 times higher 5MT concentration. A feedback insensitive form of anthranilate synthesis, the pathway specific control enzyme for tryptophan synthesis, was detected from the 5MT resistant lines. Contents of the free amino acids in five resistant lines (MR12-1 to MR12-5) showed a 7.4 to 46.6 times greater level than that in the control culture. Tryptophan, phenylalanine, and tyrosine levels in the shikimate pathway were 28.1 and 22.5 times higher in MR12-3 and MR12 4, respectively, than that measured in the control cells. Four AEC resistant cell lines were isolated from cultures grown on medium containing 1 mM AEC, They were able to grow stably with 2 mM AEC, while sensitive calli were inhibited by 0.5 mM AEC. Aspartate kinase activities of the resistant lines were insensitive to the natural inhibitor, Iysine, and accumulated 2.2 to 12.9-fold higher levels of free Iysine than that of the control cells. Especially, the levels of aspartate, asparagine, and methionine in the aspartate pathway showed higher accumulation in the AEC resistant lines than that in the control cells.

The Effect of Phosphor Thickness and Discharge Space on the Luminance and Addressing Time in ac PDP

  • Lee, Gi-Bum;Heo, Jeong-Eun;Kim, Gyu-Seup;Kim, Young-Kee;Lee, Sung-Hyun;Cho, Jung-Soo;Park, Chung-Hoo
    • Journal of KIEE
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    • v.11 no.1
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    • pp.27-32
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    • 2001
  • In this study, the luminance, luminous efficiency and address time of an PDP as a parameter of the phosphor thickness and the size of discharge space are investigated. The maximum luminance and luminous efficiency are obtained at the phosphor thickness of 30 ${\mu}{\textrm}{m}$ for the rib height of 110 ${\mu}{\textrm}{m}$. For the rib height of 120~160 ${\mu}{\textrm}{m}$, the maximum values are obtained at the thickness of 50 ${\mu}{\textrm}{m}$ regardless of the kind of R, G and B phosphor. These results do not affected by the variation of discharge space. The average decrease rate of the total charge to the phosphor thickness is about 3.35pc/cell/10 ${\mu}{\textrm}{m}$ and the average increase rate of the addressing time is about 0.027 $mutextrm{s}$/10 ${\mu}{\textrm}{m}$.

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MT-2007, Protein Kinase C Inhibitor from Aetinomycetes Isolate No. 2007-18 (방선균 분리주 No 2007-18이 생산하는 Protein Kinase C 저해물질, MT-2007)

  • 안종석;박문수;박찬선;윤병대;민태익;안순철;오원근;이현선;윤병대
    • Microbiology and Biotechnology Letters
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    • v.21 no.1
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    • pp.54-58
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    • 1993
  • During the screening of inhibitors against protein kinase CCPKC) and the bleb formation of K562 cell induced by phorbol ester from microbial secondary metabolites, MT-2007 was purified by solvent extraction, and chromatographic techniques from Actinomycetes isolate No. 2007-18. It showed completely suppression of bleb formation of K562 cell surface induced by phorbol 12.13dibutylate at the concentration of 503.9 11M and ICso on PKC was 31.4 11M. Its structure was postulated as lasalocid A sodium salt by physico-chemical properties and UV, IR. MS, IH-NMR.

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An Analysis of Forest Community and Dynamics According to Elevation in Mt. Sokri and Odae (속리산과 오대산의 해발고에 따른 산림군락 구조분석)

  • 유재은;이정호;권기원
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.5 no.4
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    • pp.238-246
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    • 2003
  • This study evaluated forest vegetation structure according to elevation in Mt. Sokri and Odae. The dominance of Pinus densiflora, Quercus variabilis, Quercus serrata, Prunus serrulata var. spontanea was apparent at elevations below 500 m. Quercus mongolica, Fraxinus sieboldiana, Acer pseudo-sieboldianum and Symplocos chinensis for. pilosa were conspicuous above 800 m. Significant P. densiflora, Rhus trichocarpa, Lindera obtusiloba and P. serrulata var. spontanea populations appeared below 700 m elevation in Mt. Odae. Q. mongolica, F. sieboldiana, A. pseudo-sieboldianum, Tilia amuresis, Abies holophylla, Carpinus cordata, Betula costata, Kalopanax pintus and Pinus koraiensis were conspicuous at elevations 1000 m and above. P. densiflora, Q. monoglica, Q. serata, R. trichocarpa and L. obtusiloba populations were significant at elevations below 700 m. Q. mongolica, F. sieboldiana, A. pseudo-sieboldianum, T. amurensis, A. holophylla, C. cordata appeared at elevations above 1000 m.

ON INTEGRAL GRAPHS WHICH BELONG TO THE CLASS $\overline{aK_{a}\;{\bigcup}\;{\beta}K_{b}}$

  • Lepovic, Mirko
    • Journal of applied mathematics & informatics
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    • v.14 no.1_2
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    • pp.39-49
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    • 2004
  • Let G be a simple graph and let $\={G}$ denotes its complement. We say that G is integral if its spectrum consists entirely of integers. If $\overline{aK_{a}\;{\bigcup}\;{\beta}K_{b}}$ is integral we show that it belongs to the class of integral graphs $[\frac{kt}{\tau}\;{x_0}\;+\;\frac{mt}{\tau}\;z}\;K_{(t+{\ell}n)+{\ell}m}\;\bigcup\;[\frac{kt}{\tau}\;{y_0}\;+\;\frac{(t\;+\;{\ell}n)k\;+\;{\ell}m}{\tau}\;z]n\;K_{em)$, where (i) t, k, $\ell$, m, $n\;\in\;\mathbb{N}$ such that (m, n) = 1, (n,t) = 1 and ($\ell,\;t$) = 1 ; (ii) $\tau\;=\;((t\;+\;{\ell}n)k\;+\;{\ell}m,\;mt)$ such that $\tau\;$\mid$kt$; (iii) ($x_0,\;y_0$) is a particular solution of the linear Diophantine equation $((t\;+\;{\ell}n)k\;+\;{\ell}m)x\;-\;(mt)y\;=\;\tau\;and\;(iv)\;z\;{\geq}\;{z_0}$ where $z_{0}$ is the least integer such that $(\frac{kt}{\tau}\;{x_0}\;+\;\frac{mt}{\tau}\;{z_0})\;\geq\;1\;and\;(\frac{kt}{\tau}\;{y_0}\;+\;\frac{(t+{\ell}n)k+{\ell}m}{\tau}\;{z_0})\;\geq\;1$.

The Energy Flow and Mineral Cycles in a Zoysia japonica and a Miscanthus sinensis Ecosystem on Mt. Kwanak 11. The Cycles of Al (관악산의 잔디와 억새 생태계에 있어서 에너지 흐름과 무기물의 순환 11.알루미늄의 순환)

  • 심규철;장남기
    • Asian Journal of Turfgrass Science
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    • v.11 no.4
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    • pp.303-309
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    • 1997
  • The investigation was cycle of aluminum of surface soil elements in dynamic grassland ecosystems at a steady state in a Zoysia japonica and a Miscanthus sinensis ecosystem in Mt. Kwanak, Korea. Average amounts of total storage for aluminum in Z japonica and M. sinensis grasslands were 8,426mg /$m^2$ and 7,849mg /$m^2$ respectively. Decay constants estimated on the base of experimental and mathematical model, were 0.04 in Z japonica grassland, and 0.08 in M. sinensis grassland. Half time to decay aluminum of litter soils were 17.33 years in Z japonica grass-land, and 8.66 years in Al. sinensis grassland. 95% decay times in Z japonica, and in M. sinensis grassland were 75.0 years and 35.0 years respectively. Needed times to lose almost all of elements in Z japonica and M. sinensis grassland were 125.0 years, and 62.50 years respectively. The metals were losed more rapidly in M. sinensis than in Z japonica grassland. The cycle of aluminum was investigated to be related with soil acidity. Key words: Cycle of aluminum, Zoysia japonica. Miscanthus sinensis, Mt. Kwanak, Decay constants, Soil acidity.

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Vertical Distribution of Forest Types on the North - western Slope of Mt. Paektu (백두산 서북사면 삼람의 수직분포)

  • Chang, Nam-Kae;Sun-Kyung Lee;Hye-Ryun Kwon
    • The Korean Journal of Ecology
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    • v.14 no.4
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    • pp.435-448
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    • 1991
  • Mt. paektu(altitude 2,749.6m) is ecologically important because successional processes following volcanic eurption are quite clear. We investigated plant communities with altitude know vertical distribution of plant vegetation on the north-western slope of Mt. paektu was divided into five parts; the pine-hardwood forest zone(600∼1,000m alt.), the lower coniferous forest zone (1,000∼1,500 m alt.), the upper coniferous forest and birch forest zone(1,500∼2,000 m alt.), the alpine shrub zone(2,000∼2,500 m alt.) and the alpine meadow zone(2,500∼2,749 m alt.). In particular, the alpine zone was divided into alpine shrub and alpine meadow zones and distributed up to 2,500m alt. these results reflet the adaptations of these shrubs at this altitude. But these shrubby communities disappeared on the alpinemeadow zone. In the alpine meadow zone above than 2,500m alt., some meadows, papaver radicatum var pscudoradicatum. bistorta ochotensis, chrysathemum zawadskii var. latilobum etc., were occured.

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Influences of the devastated forest lands on flood damages (Observed at Chonbo and the neighbouring Mt. Jook-yop area) (황폐임야(荒廢林野)가 수해참상(水害慘狀)에 미치는 영향(影響) (천보산(天寶山)과 인접(隣接) 죽엽산(竹葉山)을 중심(中心)으로))

  • Chung, In Koo
    • Journal of Korean Society of Forest Science
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    • v.5 no.1
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    • pp.4-9
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    • 1966
  • 1. On 13 September 1964 a storm raged for 3 hours and 20 minutes with pounding heavy rainfalls, and precipitation of 287.5 mm was recorded on that day. The numerous landslides were occured in the eroded forest land neighbouring Mt. Chunbo, while no landslides recorde at all on Mt. Jookyup within the premise of Kwangnung Experiment Station, the Forest Experiment Station. 2. Small-scalled Landslides were occured in 43 different places of watershed area (21.97 ha.) in which the survey had already been done, in and around Mt. Chunbo (378 m a.s.l.). The accumulated soil amount totaled $2,146,56m^3$ due to the above mentioned landslides, while soil accumulated from riverside erosion has reached to $24,168.79m^3$, consisting of soils, stones, and pebbles. However, no landslides were reported in the Mt. Jook yup area because of dense forest covers. The ratio of the eroded soil amount accumulated from the riversides to that of watershed area was 1 to 25. On the other hand, the loss and damage in the research area of Mt. Chonbo are as follows: 28 houses completly destroyed or missing 7 houses partially destroyed 51 men were dead 5 missing, and 57 wounded. It was a terrible human disaster However, no human casualties were recorded at all, 1 house-completly destroyed and missing, 2 houses-partially destroyed. Total:3 houses were destroyed or damaged, in The area of Mt. Jookyup 3. In the calculation of the quanty of accumulated soil, the or mula of "V=1/3h ($a+{\sqrt{ab}}+b$)" was used and it showed that 24, 168.79m of soil, sands, stones and pebbles carried away. 4. Average slope of the stream stood 15 at the time of accident and well found that there was a correlation between the 87% of cross-area sufferd valley erosion and the length of eroded valley, after a study on regression and correlation of the length and cross-area. In other works, the soil erosion was and severe as we approached to the down-stream, counting at a place of average ($15^{\circ}1^{\prime}$) and below. We might draw a correlation such as "Y=ax-b" in terms of the length and cross-area of the eroded valley. 5. Sites of char-coal pits were found in the upper part of the desert-like Mt. Chunbo and a professional opinion shows that the mountain was once covered by the oak three species. Furthermore, we found that the soil of both mountains have been kept the same soil system according to a research of the soil cross-area. In other words, we can draw out the fact that, originally, the forest type and soil type of both Mt. Chunbo (378m) and Mt. Jookyup (610m) have been and are the same. However, Mt. Chunbo has been much more devastated than Mt. Jookyup, and carried away its soil nutrition to the extent that the ratios of N. $P_2O_5K_2O$ and Humus C.E.C between these two mountains are 1:10;1:5 respectively. 6. Mt. Chunbo has been mostly eroded for the past 30 years, and it consists of gravels of 2mm or larger size in the upper part of the mountain, while in the lower foot part, the sandy loam was formulated due to the fact that the gluey soil has been carried and accumulated. On the hand, Mt. Jookyup has consitantly kept the all the same forest type and sandy loam of brown colour both in the upper and lower parts. 7. As for the capability of absorbing and saturating maximum humidity by the surface soil, the ratios of wet soil to dry soil are 42.8% in the hill side and lower part of the eroded Mt. Chunbo and 28.5% in the upper part. On the contrary, Mt. Jookyup on which the forest type has not been changed, shows that the ratio in 77.4% in the hill-side and 68.2% in the upper part, approximately twice as much humidity as Mt. Chunbo. This proves the fact that the forest lands with dense forest covers are much more capable of maintaining water by wood, vegitation, and an organic material. The strength of dreventing from carring away surface soil is great due to the vigorous network of the root systems. 8. As mentioned above, the devastated forest land cause not only much greater devastation, but also human loss and property damage. We must bear in mind that the eroded forest land has taken the valuable soil, which is the very existance of origin of both human being and all creatures. As for the prescription for preventing erosion of forest land, the trees for furtilization has to be planted in the hill,side with at least reasonable amount of aertilizer, in order to restore the strength of earth soil, while in the lower part, thorough erosion control and reforestation, and establishments along the riversides have to be made, so as to restore the forest type.

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