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Immunogold Labellings and Expression of Metallothionein in Regenerating Rat Liver  

Ahn, Young-Mo (Dept. of Life Science, College of Natural Science, Soonchunhyang University)
Oh, Seung-Han (Dept. of Life Science, College of Natural Science, Soonchunhyang University)
Kim, Ho-Jin (Dept. of Life Science, College of Natural Science, Soonchunhyang University)
Lee, Mi-Young (Dept. of Life Science, College of Natural Science, Soonchunhyang University)
Lee, Jong-Hwa (Dept. of Environmental Health Science, College of Natural Sciences, Soonchunhyang University)
Shin, Kil-Sang (Dept. of Life Science, College of Natural Science, Soonchunhyang University)
Kim, Wan-Jong (Dept. of Life Science, Soonchunhyang University)
Publication Information
Applied Microscopy / v.35, no.1, 2005 , pp. 15-22 More about this Journal
Abstract
Metallothionein (MT) is a low molecular weight, cysteine-rich, metal-binding, and non-enzymatic protein. The present study was carried out to investigate the expression of MT gene as well as the localization of MT in regenerating rat liver. In partial hepatectomized rats, MT mRNA was detected as early as 1 hr and reached a maximal level by 8 hr after the operation. Thereafter, this level decreased gradually until 24 hr, and it became similar to that of sham control. Meanwhile, time course of MT immunoreactivity using immunogold-labelling revealed that the number of gold particles in hepatocytes increased significantly by 12 hr, but decreased at 48 hr after partial hepatectomy. Ultrastructurally, immunogold particles indicating the presence of MT were distributed in both the cytoplasm and the nucleus of the rat hepatocytes, particularly the particles were distributed at rough endoplasmic reticulum and nucleolus and did not seem to adhere to mitochondria or lysosomes in proliferating hepatocytes. Briefly, high level of MT mRNA expression and the intense immunoreactivity and/or the specific localization of MT was observed during liver regeneration. These results suggest that MT possibly involves hepatocyte proliferation via the storage or the supply of various metal ions in the regenerating rat liver.
Keywords
Immunocytochemistry; Metallothionein; Partial hepatectomy; Rat;
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