• Journal of Nutrition and Health
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• v.48 no.5
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• pp.390-397
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• 2015

Purpose: In this study, we investigated the effects of chronic alcohol and excessive iron intake on mitochondrial DNA (mtDNA) damage and the progression of alcoholic liver injury in rats. Methods: Twenty-four Sprague-Dawley male rats were divided into four groups (Control, EtOH, Fe, and EtOH + Fe), and fed either control or ethanol (36% of total calories) liquid diet with or without 0.6% carbonyl iron for eight weeks. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, liver malondialdehyde concentrations were measured by colorimetric assays. Liver histopathology was examined by Hematoxylin-eosin staining of the fixed liver tissues. The integrity of the hepatic mtDNA and nuclear DNA was measured by long-range PCR. The gene expression levels of cytochrome c oxidase subunit 1 (Cox1) and NADH dehydrogenase subunit 4 (Nd4) were examined by real-time PCR. Results: Serum ALT and AST activities were significantly higher in the EtOH+Fe group, as compared to the Control group. Similarly, among four groups, liver histology showed the most severe lipid accumulation, inflammation, and necrosis in the EtOH + Fe group. PCR amplification of near-full-length (15.9 kb) mtDNA showed more than 50% loss of full-length product in the liver of the EtOH + Fe group, whereas amounts of PCR products of a nuclear DNA were unaffected. In addition, the changes in the mtDNA integrity showed correlation with reductions in the mRNA levels of mitochondrial gene Cox1 and Nd4. Conclusion: Our data suggested that the liver injury associated with excessive iron and alcohol intake involved mtDNA damage and corresponding mitochondrial dysfunction.

• Journal of Life Science
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• v.26 no.8
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• pp.881-886
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• 2016

Cephalanthera erecta (Thunb. ex, Murray) is an herbaceous and a member of the genus Cephalanthera in the family Orchidaceae. The species is an herbaceous and has reputed Chinese medicinal value. It has been investigated the population density and spatial distribution of this species at Mt. Ahop in Korea during 2015. The spatial pattern of C. erecta was analyzed according to several patchiness indexes, population uniformity or aggregation under different sizes of plots by dispersion indices, and spatial autocorrelation. The mean crowding (M*) and patchiness index (PAI) showed positive values except one small plot (2 m × 2 m). Most natural individuals of C. erecta for plots were not uniformly distributed in the forest community. The small plots (2 m × 2 m, to 8 m × 16 m) of C. erecta were uniformly distributed in the forest community and large plots (16 m × 16 m and 16 m × 32 m) were aggregately distributed. Significant aggregations by Moran's I of C. erecta were partially observed within IV classes (12 m). Dissimilarity among pairs of individuals could found by more than 18.0 m. In conclusion, the geographic distribution of C. erecta is not even with varying degrees of size of plots and human activities give rise to density effects in the plots at Mt. Ahop in Korea.

• The Journal of the Korea Contents Association
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• v.9 no.4
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• pp.355-363
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• 2009

Increased oxidative stress by abnormal mitochondrial function can damage cell signal transduction and gene expression, and induce insulin resistance or diabetes. Autophagy, however, improve insulin resistance by clearance of malfunctioning mitochondria. Exercise also recovers the muscle dysfunction and degeneration by activating mitochondrial biogenesis. As it seems that exercise and autophagy might act as an orchestrated network to induce mitochondrial biogenesis, we investigated whether autophagy is involved in AMPK signal pathway stimulated by exercise or AICAR to increase mitochondrial biogenesis. And it showed that PGC-1 and mtTFA, but not autophagy marker LC3 mRNA expression were significantly increased by 6 hr of acute exercise. On the other hand, PGC-1 and mtTFA mRNA expression were upregulated by AICAR treatment to C2C12 myotube. However these genes were not inhibited by LC3 siRNA transfection. These results provide the evidence that autopahgy affects on mitochondrial biogenesis through different signal pathway from AMPK signal transduction.

• Atmosphere
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• v.20 no.2
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• pp.63-71
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• 2010

In this study, we derived the wind speed at 50 m and 80 m above sea level from 567 stations over a period of 1 year and correlated to measured wind speed at 5 radiosonde sites. From these correlations, we derived and analyzed the spatial distribution of wind map over Korea based on hourly observational data recorded over a period of 5 years from 2004 to 2008. As a results, wind speed is generally high over seashores, mountains, and islands. Mean wind speed over Korea at 50 and 80 m above sea level for 5 years during 2004 to 2008 seasonally are highest at Spring, and then followed by Winter, Fall, and Summer. In 76 (14%) stations, mean wind speed at 80 m above sea level for 5 years during 2004 to 2008 are greater than $5ms^{-1}$. The prevailing winds over Korea at 80 m above sea level for 5 years during 2004 to 2008 are North (44%), Northwest (16%), and West (15%). In 99 stations, the % of wind faster than $5ms^{-1}$ was higher than 40%, and in 62 stations, the % of wind faster than $5ms^{-1}$ was higher than 50%. In 178 station, the % of prevailing winds was higher than 30%, and there are 7 stations which also have wind speeds over $5ms^{-1}$, ranking from highest to lowest, Dongsong, Daegwallyeong, Baekun Mt., Hyangrobong Mt., Sorak Mt., Gosan, and Misiryeong Mt..

• Journal of Korean Neurosurgical Society
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• v.65 no.1
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• pp.22-29
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• 2022

Objective : Despite many advancements in endovascular treatment, the benefits of mechanical thrombectomy (MT) in patients with large infarctions remain uncertain due to hemorrhagic complications. Therefore, we aimed to investigate the efficacy and safety of recanalization via MT within 6 hours after stroke in patients with large cerebral infarction volumes (>70 mL). Methods : We retrospectively reviewed the medical data of 30 patients with large lesions on initial diffusion-weighted imaging (>70 mL) who underwent MT at our institution within 6 hours after stroke onset. Baseline data, recanalization rate, and 3-month clinical outcomes were analyzed. Successful recanalization was defined as a modified treatment in cerebral ischemia score of 2b or 3. Results : The recanalization rate was 63.3%, and symptomatic intracerebral hemorrhage occurred in six patients (20%). The proportion of patients with modified Rankin Scale (mRS) scores of 0-3 was significantly higher in the recanalization group than in the non-recanalization group (47.4% vs. 9.1%, p=0.049). The mortality rate was higher in the non-recanalization group, this difference was not significant (15.8% vs. 36.4%, p=0.372). In the analysis of 3-month clinical outcomes, only successful recanalization was significantly associated with mRS scores of 0-3 (90% vs. 50%, p=0.049). The odds ratio of recanalization for favorable outcomes (mRS 0-3) was 9.00 (95% confidence interval, 0.95-84.90; p=0.055). Conclusion : Despite the risk of symptomatic intracerebral hemorrhage, successful recanalization via MT 6 hours after stroke may improve clinical outcomes in patients with large vessel occlusion.

• Journal of Forest and Environmental Science
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• v.40 no.2
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• pp.82-89
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• 2024

This study aimed to investigate habitat distribution and beetle diversity, using beetles as biological indicator species to assess environmental changes in the Mt. Mangunsan area near the South Sea of Korea. Plots were installed at varying elevations on the southern and northern slopes of Mt. Mangunsan, and the beetle species composition was determined. A total of 1,368 beetles comprising 32 species belonging to ten families were collected between May and September 2023 from the study sites in the Mt. Mangunsan area near the South Sea of Korea. The two most prevalent species consisted of Synuchus nitidus, with 152 collected beetles, and Calosoma chinense, with 128 collected beetles. In May, June, July, August, and September, 76 beetles comprising 10 species, 180 beetles comprising 18 species, 138 beetles comprising 15 species, 525 beetles comprising 27 species, and 449 beetles comprising 25 species were collected, respectively, with the highest abundance observed in August. In terms of elevation, 239 beetles comprising 19 species were collected at 300 m; 352 beetles comprising 27 species at 400 m; 314 beetles comprising 24 species at 500 m; 280 beetles comprising 22 species at 600 m; 183 beetles comprising 16 species at 700 m. Based on the slope, 442 beetles comprising 18 species belonging to eight families were collected on the southern slope, and 926 beetles comprising 24 species belonging to 10 families were collected on the northern slope. Analysis of the beetles on the southern slope produced the following estimations: diversity of 1.086, evenness of 0.866, and dominance of 0.109. Meanwhile, those in the northern slope yielded a diversity of 1.204, evenness of 0.873, and dominance of 0.081.

• Journal of applied mathematics & informatics
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• v.41 no.3
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• pp.525-540
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• 2023

In this paper, we introduce the concept of fuzzy nano M open and fuzzy nano M closed mappings in fuzzy nano topological spaces. Also, we study about fuzzy nano M Homeomorphism, almost fuzzy nano M totally mappings, almost fuzzy nano M totally continuous mappings and super fuzzy nano M clopen continuous functions and their properties in fuzzy nano topological spaces. By using these mappings, we can able to extended the relation between normal spaces and regular spaces in fuzzy nano topological spaces.

• Geophysics and Geophysical Exploration
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• v.9 no.4
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• pp.261-270
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• 2006

Two-dimensional (2-D) inversion of magnetotelluric (MT) data for two survey lines having south-north direction from Jeju Island has been carried out. Broad band MT sounding curves with good quality could be gathered by performing audio-frequency magnetotelluric (AMT) survey during the MT survey and by operating the remote reference in Kyushu Island, Japan. Comparison of the 2-D inversion model using MT band only and that using both AMT and MT bands for the field data as well as for the data from numerical 2-D modeling said that high frequency information from AMT survey can be useful for interpreting not only the shallow part but also the deep structures, especially when the formation is resistive. The 2-D inversion models of field data show a thick layer having around 10 ohm-m in the depth of a few hundred meters throughout the survey area, which can be considered as the unconsolidated sedimentary layer. And they also show a conductive anomaly at the central part of each survey lines. It can be either the effect of the surrounding sea water, or the structures due to ancient volcanic events. But unfortunately by now, we do not have any further information about the anomaly.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.6
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• pp.857-866
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• 2011

This study examined the metabolism of free radicals in hepatic mitochondria of goats induced by lipopolysaccharide (LPS), and investigated the effects of melatonin (MT). Forty-eight healthy goats ($10{\pm}1.2\;kg$) were randomly selected and divided into four groups: saline control, LPS, MT+LPS and MT. The goats within each group were3 sacrificed either 3 or 6 h after treatment and the livers removed to isolate mitochondria. The respiration control ratio (RCR), the ADP:O ratio, the oxidative phosphorylation ratio (OPR), the concentration of $H_2O_2$ and the activities of Complex I-IV were determined. The mitochondrial membrane potential ($\Delta\psi_m$) was analyzed by flow cytometry. The results showed that RCR, O/P and OPR of the LPS group decreased (p<0.05), as well as activities of respiratory complexes, whereas the generation of $H_2O_2$ in Complex III increased (p<0.05) after 3 h, while Complex II and III increased after 6 h. Also, it was found that the mitochondrial membrane potential of the LPS group declined (p<0.05). However, pre-treatment with MT attenuated the injury induced by LPS, which not only presented higher (p<0.05) RCR, O/P, OPR, and respiratory complex activities, but also maintained the $\Delta\psi_m$. Interestingly, it is revealed that, in the MT+LPS group, the generation of $H_2O_2$ increased firstly in 3 h, and then significantly (p<0.05).decreased after 6 h. In the MT group, the function of mitochondria, the transmenbrane potential and the generation of $H_2O_2$ were obviously improved compared to the control group. Conclusion: melatonin prevents damage caused by LPS on hepatic mitochondria of goats.

• Korean Journal of Plant Taxonomy
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• v.42 no.1
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• pp.80-90
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• 2012

While preparing a floristic study of Korean hepatics, we discovered the following 10 unrecorded species from Mt. Jiri-san (maximum height, 1,915 m) and Mt. Seorak-san (maximum height, 1,708 m): Neotrichocolea bissetii (Mitt.) S. Hatt., Calypogeia angusta Steph., Cephaloziella massalongii K. Mller, Harpanthus scutatus (F. Weber & D. Mohr) Spruce, Plectocolea torticalyx (Steph.) S. Hatt., Anastrophyllum michauxii (F. Weber) H. Buch, Lophozia excisa (Dicks.) Dumort., Lophozia lantratoviae Bakalin, Mylia verrucosa Lindb., Scapania sphaerifera H. Buch. They are distributed near streams and/or on the top of a ridge on Mt. Jiri-san and Mt. Seorak-san.