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Growth Inhibition of Cucumber by Absorbing Excess Al at Low Soil pH (강한 산성토양에서 Al의 과잉 흡수에 의한 오이 생육장해 양상)

  • Kim, Yoo-Hak;Kim, Myung Sook;Kang, Seong Soo;Lee, Hyeong Yong
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.6
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    • pp.925-927
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    • 2012
  • On-site diagnosis applied to soil having a symptom of yellowing and whitening in cucumber leaf and stem. Soil pH determined 4.2 by methods of on-site analysis and 4.5 by soil test analysis. High aluminum in soil solution extracted with water saturation was detected. Leaf and stem tissue were abundant in Al content but not in Ca. Also, N content of leaf and stem was low compared to normal N ranges. This symptom of cucumber assumed to be from the Al and nitrous acid gas toxicity by low soil pH and Eh. Conclusionally, symptom in leaf and stem of cucumber was alleviated and cucumber normally recovered during cultivation period by applying calcium hydroxide solution to correct soil pH up to 6.5. These results showed that low soil pH resulted in aluminum toxicity and N deficiency to plant growth in on-site farming.

Infrared Thermographic Imaging in Patients with Alopecia (탈모 환자의 적외선 체열 진단상 안면부 체열 특성)

  • Yi Tae-Hoo;Moon Jung-Bae;An Kyung-Eh;Lee Hye-Jung
    • The Journal of Korean Medicine
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    • v.27 no.2 s.66
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    • pp.244-252
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    • 2006
  • Objectives : To examine any potential relationships between the types of alopecia and the facial heat distribution in patients with alopecia. Methods : 183 patients with alopecia participated in this study to provide facial heat distribution measured by the Infrared Thermography Scanner (ITS, Nec San-ei Instruments Ltd, Japan). The thermography scan was used in a light- and heat-protected room after 20 minutes' of rest. 1.5m of distance was maintained between the patients and the scanner. Results : Specificity in the type of facial heat distribution was found as follow. 1. Types of facial heat distribution can be classified as T-type and diffused patterns. 2. There was a significant difference in the pattern of facial heat distribution among different types of alopecia (p=0.002): facial heat distribution appeared T-type in androgenic alopecia, alopecia areata, and telogen effluvium (71.3%, 85.7%, 70.4%), whereas diffused pattern was dominant in seborrheic alopecia (55.6%). 3. There was a significant difference in the pattern of facial heat distribution between men and women (p<0.001) : While the T-type and diffused type appeared equally in men (50.6% : 49.4%), T-type was dominant in women (88.0% vs. 12.0%). Conclusions : We conclude that the pattern of facial heat distribution differs depending on the types of alopecia and gender. These differences may provide useful information for diagnosis and clinical therapy for this population.

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Effect of Cerium Ammonium Nitrate and Alumina Abrasive Particles on Polishing Behavior in Ruthenium Chemical Mechanical Planarization (Ruthenium CMP에서 Cerium Ammonium Nitrate와 알루미나 연마 입자가 연마 거동에 미치는 영향)

  • Lee, Sang-Ho;Lee, Sung-Ho;Kang, Young-Jae;Kim, In-Kwon;Park, Jin-Goo
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.18 no.9
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    • pp.803-809
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    • 2005
  • Cerium ammonium nitrate (CAN) and nitric acid was used an etchant and an additive for Ru etching and polishing. pH and Eh values of the CAN and nitric acid added chemical solution satisfied the Ru etching condition. The etch rate increased linearly as the concentration of CAN increased. Nitric acid added solution had the high etch rate. But micro roughness of etched surfaces was not changed before and after etching, The removal rate of Ru film was the highest in $1wt\%$ abrasive added slurry, and not increased despite the concentration of alumina abrasive increased to $5wt\%$. Even Ru film was polished by only CAN solution due to the friction. The highest removal rate of 120nm/min was obtained in 1 M nitric acid and $1wt\%$ alumina abrasive particles added slurry. The lowest micro roughness value was observed in this slurry after polishing. From the XPS analysis of etched Ru surface, oxide layer was founded on the etched Ru surface. Therefore, Ru was polished by chemical etching of CAN solution and oxide layer abrasion by abrasive particles. From the result of removal rate without abrasive particle, the etching of CAN solution is more dominant to the Ru CMP.

Infrared thermographic imaging in patients with alopecia. (탈모 환자의 적외선 체열 진단상 안면부 체열 특성)

  • Yi, Tae-Hoo;Moon, Jung-Bae;An, Kyung-Eh;Lee, Hye-Jung
    • Journal of Oriental Medical Thermology
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    • v.5 no.1
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    • pp.78-86
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    • 2006
  • Objectives: To examine any potential relationships between the types of alopecia and the facial heat distribution in patients with alopecia Methods: 183 patients with alopecia participated in this study to provide facial heat distribution measured by the Infrared Thermography Scanner (ITS, Nec San-ei Instruments Ltd, Japan). The thermography scan was used in a light-and heat-protected room after 20 minutes’of resting period. 1.5m of distance maintained between the patients and ITS. Results: Specificity in the type of facial heat distribution was found as follow. 1. Types of facial heat distribution can be classified as T-type and diffused patterns. 2. There was a significant difference in the pattern of facial heat distribution among different types of alopecia (p=0.002): facial heat distribution appeared T-type in Androgenic Alopecia, Alopecia Areata, and Telogen Effluvium (71.3%, 85.7%, 70.4%), whereas diffused pattern was dominant in Seborrheic Alopecia(55.6%). 3. There was a significant difference in the pattern of facialheat distribution between men and women (p<0.001): While the T-type and diffused type were equally appeared in men (50.6%: 49.4%), T-type was dominant in women (88.0% vs. 12.0%). Conclusions: We conclude that the pattern of facial heat distribution differs depends on the types of alopecia and gender. These differences may provide useful information for the diagnosis and clinical therapy for this population.

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Effect of Soil Texture and Tillage Method on Rice Yield and Methane Emission during Rice Cultivation in Paddy Soil

  • Cho, Hyeon-Suk;Seo, Myung-Chul;Kim, Jun-Hwan;Sang, Wan-gyu;Shin, Pyeong;Lee, Geon Hwi
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.5
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    • pp.564-571
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    • 2016
  • As the amount of rice straw collected increases, green manure crops are used to provide the needed organic matter. However, as green manure crops generate methane in the process of decomposition, we tested with different tillage depths in order to reduce the emission. The atmosphere temperature of the chamber was $25{\sim}40^{\circ}C$ during the examination of methane and soil temperature was $2{\sim}10^{\circ}C$ lower than air temperature. The redox potential (Eh) of the soil drastically fell right before irrigated transplanting and showed -300~-400 mV during the cultivating period of rice (7~106 days after transplant). When hairy vetch was incorporated to soil and the field was not irrigated, the generation of methane did not occur from 12 to 4 days before transplanting rice and started after irrigation. Regarding the pattern of methane generation during the cultivation of rice, methane was generated 7 days after transplanting, reached the pinnacle at by 63~74 days after transplanting, rapidly decreased after 86~94 days past transplanting and stopped after 106 days past transplanting. When tested by different soil types, methane emission gradually increased in loam and clay loam during early transplant, whereas it sharply increased in sandy loam. The total amount of methane emitted was highest in sandy loam, followed by loam and clay loam. In all three soil types, methane emission significantly reduced when tillage depth was 20 cm compared to 10 cm. The rice growths and yield were not affected by tillage depth. Therefore, reduction of methane emission could be achieved when application hairy vetch to the soil with tillage depth of 20 cm in paddy soil.

A Study on the Geochemical Clogging for the Assessment of the Hydrological Safety of the Underground Oil Storage Carvern (지하유류비축기지 수리안정성 평가를 위한 광물학적 클로깅 가능성 연구)

  • Kim, Geon-Young;Bae, Dae-Seok;Choi, Byeong-Young;Oh, Se-Joong;Koh, Yong-Hwon
    • Journal of the Mineralogical Society of Korea
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    • v.21 no.2
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    • pp.139-159
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    • 2008
  • Geochemical analysis of the various kinds of water including observation borehole groundwater was carried out for the assessment of the hydrological safety of the underground oil storage cavern and the potentiality of mineralogical and microbiological clogging was estimated. Most of water samples belonged to $Ca-HCO_3$ and $Ca-HCO_3-SO_4$ types. There was no distinct chemical difference in the various kinds of water. All kinds of water are undersaturated with the calcite which is the major clogging mineral. Most water samples have low Fe and Mn concentrations. However, they are saturated or oversaturated with the iron-oxide/hydroxide minerals and have high dissolved oxygen contents which suggests the possibility of clogging by the iron-oxide/hydroxide minerals as a long-term aspect. Several water samples from the ground observation borehole also show the high saturation indices far the clay minerals, which can fill up the fractures, indicating the possibility of clogging by the clay minerals. Statistical analysis shows the degree of mineral precipitation or dissolution is mainly controlled by pH, Eh and DO of water samples. According to the microbial analysis, the aerobic microbes and slime forming bacteria are dominant in most water samples and anaerobic microbes including sulfate reducing bacteria are very low or not detected. Although the slime forming bacteria which are known as a main microbial cause of the clogging is lower than $10^5\;CFUs/mL$ in all water samples, because the slime forming bacteria are dominant microbe in several observation boreholes, the clogging can be caused by it as a long-term aspect. In addition, the possibility of clogging can be increased if the microbial effect is combined with the mineralogical effect such as iron oxide/hydroxide minerals for the possibility of clogging. Therefore, the systematic and long-term program for the assessment of clogging is required for the safe operation of underground oil storage cavern.

Analysis of Bacterials Community Structure in Leadchate-Contaminated Groundwater using Denaturing Gradient Gel Electrophoresis (Denaturing Gradient Gel Electrophoresis를 이용한 매립지 침출수로 오염된 지하수의 세균 군집 분석)

  • Kim Jai-Soo;Kim Ji-Young;Koo So-Yeon;Ko Kyung-Seok;Lee Sang-Don;Cho Kyung-Suk;Koh Dong-Chan
    • Microbiology and Biotechnology Letters
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    • v.34 no.2
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    • pp.166-173
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    • 2006
  • This research has been performed to clarify the relationship between hydrogeochemistry and bacterial community structure in groundwater contaminated with landfill leachate. We collected and analyzed samples from 5 sites such as leachate (KSG1-12), treated leachate (KSG1-16), two contaminated groundwaters (KSG1-07 and KSG1-08) and non-contaminated groundwater (KSG1-13). pH was 8.83, 8.04, 6.87, 6.87 and 6.53 in order; redox potential (Eh) 108, 202, 47, 200 and 154 mV; electric conductivity (EC) 3710, 894, 1223, 559 and 169.9 $\mu$S/cm; suspended solids (SS) 86.45, 13.74, 4.18, 0.24 and 11.91 mg/L. In KSG01-12, the ion concentrations were higher especially in $Cl^-$ and $HCO_3^-$ than other sites. The concentrations of Fe, Mn and $SO_4^{2-}$ were higher In KSG1-07 than in KSG1-08, and vise versa in $NO_3^{2-}$. In the comparison of DGGE fingerprint patterns, the similarity was highest between KSG1-13 and KSG1-16 (57.2%), probably due to common properties like low or none contaminant concentrations. Otherwise KSG1-08 showed lowest similarities with KSG1-13 (25.8%) and KSG1-12 (27.6%), maybe because of the degree of contamination. The most dominant bacterial species in each site were involved in $\alpha$-Proteobacteria (55.6%) in KSG1-12, $\gamma$-Proteobacteria (50.0%) in KSG1-16, $\beta$-Proteobacteria (66.7%) in KSG1-07, $\gamma$-Proteobacteria (54.5%) in KSG1-08 and $\beta$-Proteobacteria (36.4%) in KSG1-13. These results indicate that the microbial community structure might be changed according to the flow of leachate in grounderwater, implying changes in concentrations of pollutants, available electron accepters and/or other environmental conditions.

Status and Implications of Hydrogeochemical Characterization of Deep Groundwater for Deep Geological Disposal of High-Level Radioactive Wastes in Developed Countries (고준위 방사성 폐기물 지질처분을 위한 해외 선진국의 심부 지하수 환경 연구동향 분석 및 시사점 도출)

  • Jaehoon Choi;Soonyoung Yu;SunJu Park;Junghoon Park;Seong-Taek Yun
    • Economic and Environmental Geology
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    • v.55 no.6
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    • pp.737-760
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    • 2022
  • For the geological disposal of high-level radioactive wastes (HLW), an understanding of deep subsurface environment is essential through geological, hydrogeological, geochemical, and geotechnical investigations. Although South Korea plans the geological disposal of HLW, only a few studies have been conducted for characterizing the geochemistry of deep subsurface environment. To guide the hydrogeochemical research for selecting suitable repository sites, this study overviewed the status and trends in hydrogeochemical characterization of deep groundwater for the deep geological disposal of HLW in developed countries. As a result of examining the selection process of geological disposal sites in 8 countries including USA, Canada, Finland, Sweden, France, Japan, Germany, and Switzerland, the following geochemical parameters were needed for the geochemical characterization of deep subsurface environment: major and minor elements and isotopes (e.g., 34S and 18O of SO42-, 13C and 14C of DIC, 2H and 18O of water) of both groundwater and pore water (in aquitard), fracture-filling minerals, organic materials, colloids, and oxidation-reduction indicators (e.g., Eh, Fe2+/Fe3+, H2S/SO42-, NH4+/NO3-). A suitable repository was selected based on the integrated interpretation of these geochemical data from deep subsurface. In South Korea, hydrochemical types and evolutionary patterns of deep groundwater were identified using artificial neural networks (e.g., Self-Organizing Map), and the impact of shallow groundwater mixing was evaluated based on multivariate statistics (e.g., M3 modeling). The relationship between fracture-filling minerals and groundwater chemistry also has been investigated through a reaction-path modeling. However, these previous studies in South Korea had been conducted without some important geochemical data including isotopes, oxidationreduction indicators and DOC, mainly due to the lack of available data. Therefore, a detailed geochemical investigation is required over the country to collect these hydrochemical data to select a geological disposal site based on scientific evidence.

Expression of Cell Cycle Related Genes in HL60 Cells Undergoing Apoptosis by X-irradiation (HL60 세포주에서 방사선 조사에 의한 Apoptosis와 세포 주기 관련 유전자의 발현 변화)

  • Kim, Jin-Hee;Park, In-Kyu
    • Radiation Oncology Journal
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    • v.16 no.4
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    • pp.377-388
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    • 1998
  • Purpose : To evaluate changes in expression of cell cycle related genes during apoptosis induced in HL60 cells by X-irradiation to understand molecular biologic aspects in mechanism of radiation therapy. Material and Methods : HL-60 cell line (promyelocytic leukemia cell line) was grown in culture media and irradiated with 8 Gr by linear accelerator (6 MV X-ray). At various times after irradiation, ranging from 3 to 48 hours were analyzed apoptotic DNA fragmentation assay for apoptosis and by western blot analysis and semi-quantitative RT-PCR for expression of cell cycle related genes (cyclin A, cyclin B, cyclin C, cyclin Dl, cyclin E, cdc2, CDK2, CDK4, $p16^{INK4a}$, $p21^{WAF1}$, $p27^{KIP1}$, E2F, PCNA and Rb). Results : X-irradiation (8 Gy) induced apoptosis in HL-60 cell line. Cycline A protein increased after reaching its peak 48 h after radiation delivery and cyclin E, E2F, CDK2 and RB protein increased then decreased after radiation. Radiation induced up-regulation of the expression of E2F is due to mostly increase of Phosphorylated retinoblastoma proteins (ppRb). Cyclin Dl, PCNA, CDC2, CDK4 and $p16^{INK4a}$ protein underwent no significant change at any times after irradiation. There was not detected $p21^{WAF1}$ and $p27^{KIP1}$ protein. Cyclin A, B, C mRNA decreased immediately after radiation and then increased at 12 h after radiation. Cyclin Dl mRNA increased immediately and then decreased at 48 h after radiation. After radiation, cyclin E mRNA decreased with the lapse of time. CDK2 mRNA decreased at 3h and increased at eh after radiation. CDK4 mRNA rapidly increased at 6 to 12 h after radiation. There was no change of expression of $p16^{INK4a}$ and not detected in expressin of $p21^{WAF1}$ and $p27^{KIP1}$ mRNA. Conclusion : We suggest that entry into S phase may contribute to apoptosis of HL60 cells induced by irradiation. Increase of ppRb and decrease of pRb protein are related with radiation induced auoptosis of HL60 cells and tosis of HL60 cells induced by irradiation. Increase of ppRb and decrease of PRb protein are related with radiation induced apoptosis of HL60 cells and this may be associated with induction of E2F and cyclinE/CDK2. These results support that $p21^{WAF1}$ and $p27^{KIP1}$ are not related with radiation induced-apoptosis.

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Study on Temporal Decay Characteristics of Naturally Occurring Radionuclides in Groudwater in Two Mica Granite Area (복운모화강암지역 지하수 중 자연방사성 물질의 경시적 붕괴특성 연구)

  • Kim, Moon Su;Kim, Tae Seung;Kim, Hyun Koo;Kim, Dong Su;Jeong, Do Hwan;Ju, Byoung Kyu;Hong, Jung Ki;Kim, Hye Jin;Park, Sun Hwa;Jeong, Chan Ho;Cho, Byong Wook;Han, Jin Seok
    • Journal of Soil and Groundwater Environment
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    • v.18 no.4
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    • pp.19-31
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    • 2013
  • To figure out the decay characteristics of naturally occurring radionuclides, eight sampled groundwaters from a monitoring borehole having high levels of uranium and radon concentrations in a two mica granitic area have analyzed by liquid scintillation counters (LSC) for over 1 year. In December 2011, three groundwater samples (DJ1, DJ2, DJ3) were obtained from each aquifer system located at -20 m, -40 m, -60 m of the monitoring borehole below the ground surface, respectively. Five samples (DJ4, DJ5, DJ6, DJ7, DJ8) were additionally gained from each aquifer positioned -20 m, -40 m, -60 m, -100 m, -105 m of the borehole in February 2012, respectively. Temporal variation characteristics of uranium and radon concentrations have showed over maximum 2.1 times and 1.4 times fluctuations of the values in the same sampling intervals over time, respectively. The intervals of -40 m and -105 m in the borehole have the highest values of uranium and radon concentrations, respectively. This may imply that the concentrations of naturally occurring radionuclides such as uranium and radon in groundwater have been changed over time and indicate that the qualities of groundwaters from the aquifers developed at each interval in the borehole are different each other. This discrepancy, moreover, could be caused by behaviour differences between uranium which is in ionic status having a half life of 4.6 billion years and is transported along with the flowing groundwater, and radon which is in gaseous status having a 3.82 day's half life in the aquifer systems. Physicochemical characteristics of groundwaters from the aquifer systems could be identified by the results of the on-situ measuring items such as pH and Eh, and the major ionic contents. The CPM values of eight groundwater samples analysed by LSC over one year have shown not to follow the theoretical decay curve of the radon. The CPM values of the samples have ranged from 2 to 7.5 after it had passed two months when the theoretical CPM values of the radon started zero since the initial analysis. Alpha and beta particle spectrums have shown the peaks of radium-226, however they have not revealed any peaks of radon and it's daughter products such as polonium-218 and 214, bismuth-214 for the late stage of the analysis. This implies that the groundwater from the borehole may contain radium-226 having a half life of 1,600 years which decays continuously.