• Transactions of the Korean Society of Machine Tool Engineers
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• v.13 no.2
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• pp.69-74
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• 2004

In general the surge pressure generated in hydraulic systems causes noise, vibration and odd effect to the system. To reduce the surge pressure, high frequency PWM control of 20KHz was attempted. To estimate the braking noise caused by surge, a vehicle equipped with on-board ABS hydraulic modulator has been experimented with respect to the various breaking condition. Thorough this experiments, it was found that breaking noise has been reduced using high frequency PWM control method compare with low frequency method. To evaluate high frequency control m practice, including verification of general functionality, EMI tests was experimented. Its was found that it is necessary to have the solution to electromagnetic interference(EMI) generated by switching elements.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.19 no.3
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• pp.543-552
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• 1994

Dynamic Time Warp(DTW) needs for iterative calculations and the design of PE cell suitable for the operations is very important. Accordingly, this paper aims at real time recognition design enables large dictionary hardware realization using DTW algorithm. The DTW PE cell separated into three large blocks. "MIN" is the one block for counting accumulated minimum distance. "ADD" block calculates these minimum distances, and "ABS" seeks for the absolute values to the total sum of local distances. Circuit design and verification about the three block have been accomplished, and performed layout '||'&'||' DRC(design rule check) using 1.2 m CMOS N-Well rule base.CMOS N-Well rule base.

• Parasites, Hosts and Diseases
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• v.55 no.6
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• pp.623-630
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• 2017

Plasmodium lactate dehydrogenase (pLDH) is a strong target antigen for the determination of infection with Plasmodium species specifically. However, a more effective antibody is needed because of the low sensitivity of the current antibody in many immunological diagnostic assays. In this study, recombinant Plasmodium vivax LDH (PvLDH) was experimentally constructed and expressed as a native antigen to develop an effective P. vivax-specific monoclonal antibody (mAb). Two mAbs (2CF5 and 1G10) were tested using ELISA and immunofluorescence assays (IFA), as both demonstrated reactivity against pLDH antigen. Of the 2 antibodies, 2CF5 was not able to detect P. falciparum, suggesting that it might possess P. vivax-specificity. The detection limit for a pair of 2 mAbs-linked sandwich ELISA was 31.3 ng/ml of the recombinant antigen. The P. vivax-specific performance of mAbs-linked ELISA was confirmed by in vitro-cultured P. falciparum and P. vivax-infected patient blood samples. In conclusion, the 2 new antibodies possessed the potential to detect P. vivax and will be useful in immunoassay.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.4
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• pp.600-604
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• 2001

Immunoassay was used to study the detection method of irradiated shrimp. Sandwich ELISA was formatted with monoclonal antibody (Ab) (M-IgG) and polyclonal Abs (P-IgG) and polyclonal Abs (P-IgG) individually produced against brown shrimp tropomyosin (TPM) as an antigen. When M-IgG was used as a coating Ab to capture TPM, and P-IgG were used as reaction Ab against captured TPM could be detected in the range of 12.5 to 50 $\mu\textrm{g}$/mL. Detected concentrations of TPM from irradiated shrimp decreased dose-dependently, and the concentration of Ag by combination of irradiation with heating or freezing treatments also decreased. This results suggests the possibility for Sandwich ELISA, one of immunological analyses, to be applied for detecting irradiated shrimp.

• Journal of Life Science
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• v.12 no.4
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• pp.460-463
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• 2002

Twelve clones of monoclonal antibodies (mAbs) against thyroglobulin were produced and characterized. Among them, three mAbs (TN-1, TN-2 and TN-3) showed high binding affinity to thyroglobulin. from ELISA inhibition assay, TN-2 showed considerable reactivity with soluble thyroglobulin. TN-2 also reacted with phosphatidylserine which has a negative charge in aqueous condition. These results suggest that TN-2 has characteristics of autoantibody concerned with thyroiditis.

• Parasites, Hosts and Diseases
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• v.52 no.2
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• pp.201-204
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• 2014

This study was undertaken to characterize the properties of a 100 kDa somatic antigen from Metagonimus yokogawai. Monoclonal antibodies (mAbs) were produced against this 100 kDa antigen, and their immunoreactivity was assessed by western blot analysis with patients' sera. The mAbs against the 100 kDa antigen commonly reacted with various kinds of trematode antigens, including intestinal (Gymnophalloides seoi), lung (Paragonimus westermani), and liver flukes (Clonorchis sinensis and Fasciola hepatica). However, this mAb showed no cross-reactions with other helminth parasites, including nematodes and cestodes. To determine the topographic distribution of the 100 kDa antigen in worm sections, indirect immunoperoxidase staining was performed. A strong positive reaction was observed in the tegumental and subtegumental layers of adult M. yokogawai and C. sinensis. The results showed that the 100 kDa somatic protein of M. yokogawai is a common antigen which recognizes a target epitope present over the tegumental layer of different trematode species.

• Structural Engineering and Mechanics
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• v.88 no.6
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• pp.521-533
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• 2023

In the present study, the finite volume method is applied for the thermal performance prediction of the natural ventilation system using vertical solar chimney whereas, design parameters are optimized through the response surface methodology (RSM). The computational simulations are performed for various parameters of the solar chimney such as absorber temperature (40≤T_abs≤70℃), inlet temperature (20≤T₀≤30℃), inlet height of (0.1≤h≤0.2 m) and chimney width (0.1≤d≤0.2 m). Analysis of variance (ANOVA) was carried out to identify the design parameters that influence the average Nusselt number (Nu) and mass flow rate (ṁ). Then, quadratic polynomial regression models were developed to predict of all the response parameters. Consequently, numerical and graphical optimizations were performed to achieve multi-objective optimization for the desired criteria. According to the desirability function approach, it can be seen that the optimum objective functions are Nu=25.67 and ṁ=24.68 kg/h·m, corresponding to design parameters h=0.18 m, d=0.2 m, T_abs=46.81℃ and T₀=20℃. The optimal ventilation flow rate is enhanced by about 96.65% compared to the minimum ventilation rate, while solar energy consumption is reduced by 49.54% compared to the maximum ventilation rate.

• Horticultural Science & Technology
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• v.32 no.3
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• pp.318-329
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• 2014

In order to gain insight into the physiological responses of plants to high temperature stress, the effects of temperature on Chinese cabbage (Brassica campestris subsp. napus var. pekinensis cv. Detong) were investigated through analyses of photosynthesis and chlorophyll fluorescence under 3 different temperatures in the temperature gradient tunnel. Growth (leaf length and number of leaves) during the rosette stage was greater at ambient $+4^{\circ}C$ and ambient $+7^{\circ}C$ temperatures than at ambient temperature. Photosynthetic $CO_2$ fixation rates of Chinese cabbage grown under the different temperatures did not differ significantly. However, dark respiration rate was significantly higher in the cabbage that developed under ambient temperature relative to elevated temperature. Furthermore, elevated growth temperature increased transpiration rate and stomatal conductance resulting in an overall decrease of water use efficiency. The chlorophyll a fluorescence transient was also considerably affected by high temperature stress; the fluorescence yield $F_J$, $F_I$, and $F_P$ decreased considerably at ambient $+4^{\circ}C$ and ambient $+7^{\circ}C$ temperatures, with induction of $F_K$ and decrease of $F_V/F_O$. The values of RC/CS, ABS/CS, TRo/CS, and ETo/CS decreased considerably, while DIo/CS increased with increased growth temperature. The symptoms of soft-rot disease were observed in the inner part of the cabbage heads after 7, 9, and/or 10 weeks of cultivation at ambient $+4^{\circ}C$ and ambient $+7^{\circ}C$ temperatures, but not in the cabbage heads growing at ambient temperature. These results show that Chinese cabbage could be negatively affected by high temperature under a future climate change scenario. Therefore, to maintain the high productivity and quality of Chinese cabbage, it may be necessary to develop new high temperature tolerant cultivars or to markedly improve cropping systems. In addition, it would be possible to use the non-invasive fluorescence parameters $F_O$, $F_V/F_M$, and $F_V/F_O$, as well as $F_K$, $M_O$, $S_M$, RC/CS, ETo/CS, $PI_{abs}$, and $SFI_{abs}$ (which were selected in this study), to quantitatively determine the physiological status of plants in response to high temperature stresses.

• Parasites, Hosts and Diseases
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• v.37 no.4
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• pp.249-256
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• 1999

A total of 198 sera from stray cats was assayed against Toxoplasma gondii antigen by western blot. Out of 198 sera assayed, 26 sera (13.1％) showed typical blot patterns against T gondii. When spotted by ELISA absorbance and indirect latex agglutination lest (ILAT) titer, all 26 cases were distributed over the cut-off value of ELISA whereas 24 cases (92.3％) were in the positive range of 1:32 or higher and 2 cases in negative range by ILAT. Among western blot negative 172 sera, 162 cases were negative in both ILAT and ELISA while 10 cases were reactive falsely such that three cases were ILAT positive with 1:32 titer and 9 cases were ELISA positive (2 cases overlapped). These 10 cases reacted peculiarly without typical binding pattern in Western blot. Sandwich-ELISA was performed with monoclonal antibodies (mAbs) of Tg563 (30 kDa, SAG 1), Tg505 (22 kDa, SAG2), Tg605 (43 kDa, SAG3), Tg556 (28 kDa, GRA2), Tg737 (32 kDa, GRA6). Tg695 (66 kDa, ROP2), Tg786 (42 kDa, ROP6), and Tg621 (32 kDa, anonymous but cytosolic) clone, respectively. All western blot-positive cases were in the positive range and negative cases in the negative range clearly. Among the 10 false reactive cases, 3 cases were in the positive range with one or more mAbs. All mAbs used in this study were confirmed to be specific to T. gondii infection as a standardized sandwich-ELISA to differentiate it from other pathogens.

• Pediatric Infection and Vaccine
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• v.30 no.1
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• pp.1-11
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• 2023

Respiratory syncytial virus (RSV) is a pathogen with a high burden of disease and social cost among infants worldwide, but the development of a vaccine has been delayed. The recent understanding of the pathogenesis of RSV, progress in reverse genetics, and successful implementation of other maternal immunizations have prompted the recent rapid development of monoclonal antibodies (mAbs) and vaccines for RSV prevention. Phase 3 clinical trials for two next-generation mAbs (nirsevimab and clesrovimab) and two maternal RSV pre-F vaccines are currently underway or have been recently completed. Soon, we might be able to protect young infants through long-acting mAbs and/or maternal immunization. Additionally, the development of live-attenuated vaccine candidates that are capable of avoiding enhanced RSV disease is ongoing. We need to gain familiarity with these newly developed strategies and collect epidemiological data on domestic RSV to adequately prepare for a new era of RSV prevention.