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http://dx.doi.org/10.5352/JLS.2002.12.4.460

Production and characterization of anti-thyrog1obulin monoclonal antibodies  

남경수 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터)
손윤희 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터)
백태선 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터)
김철호 (동국대학교 한의과대학 생화학교실)
임종국 (동국대학교 한의과대학 경혈학교실)
황철원 (한동대학교 환경미생물학교실)
Publication Information
Journal of Life Science / v.12, no.4, 2002 , pp. 460-463 More about this Journal
Abstract
Twelve clones of monoclonal antibodies (mAbs) against thyroglobulin were produced and characterized. Among them, three mAbs (TN-1, TN-2 and TN-3) showed high binding affinity to thyroglobulin. from ELISA inhibition assay, TN-2 showed considerable reactivity with soluble thyroglobulin. TN-2 also reacted with phosphatidylserine which has a negative charge in aqueous condition. These results suggest that TN-2 has characteristics of autoantibody concerned with thyroiditis.
Keywords
Thyroglobulin; autoantigen; autoimmune disease; anti-thyroglobulin monoclonal antibody;
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