• Journal of Microbiology and Biotechnology
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• v.17 no.7
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• pp.1221-1225
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• 2007

A ${\beta}$-ionone-resistant mutant strain isolated from the red yeast Xanthophyllomyces dendrorhous KCTC 7704 was used for batch and continuous fermentation kinetic studies with glucose media in a 2.5-1 jar fermentor at $22^{\circ}C$ and pH 4.5. The kinetic pattern of growth and carotenoid concentration in the batch fermentations exhibited a so-called mixed-growth-associated product formation, possibly due to the fact that the content of intracellular carotenoids depends on the degree of physical maturation toward adulthood. To determine the maximum specific growth rate constant (${\mu}_m$) and Monod constant ($K_s$) for the mutant, glucose-limited continuous culture studies were performed at different dilution rates within a range of $0.02-0.10\;h^{-1}$. A reciprocal plot of the steady-state data (viz., reciprocal of glucose concentration versus residence time) obtained from continuous culture experiments was used to estimate a ${\mu}_m$ of $0.15\;h^{-1}$ and $k_s$ of 1.19 g/l. The carotenoid content related to the residence time appeared to assume a typical form of saturation kinetics. The maximum carotenoid content ($X_m$) for the mutant was estimated to be $1.04\;{\mu}g/mg$ dry cell weight, and the Lee constant ($k_m$), which was tentatively defined in this work, was found to be 3.0 h.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.12 no.4
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• pp.190-195
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• 2002

NaX zeolite crystals of a uniform particle size of 50 $\mu$m were grown by continuous crystallization method from seed crystals (10~20 $\mu$m) added into a 0.5~2.0 g mother liquor having a composition $3.5Na_2O : Al_2O_3: 2.1SiO_2: 1000H_2O$. In order to investigate the crystal growing by continuous method, the mother solution was supplied after 7 days, 5 days, 3 days and 1 day, respectively. The seeding resulted in an increase in the fraction of large crystals compared with unseeded batches and successfully led to an uniform NaX zeolite crystal. It was postulated that the seeding in the synthesis mixture leaded out increase of surface area for physical contact reaction and directed growth of seed crystal without the nucleation in the synthesis gel.

• Bulletin of the Korean Chemical Society
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• v.24 no.12
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• pp.1775-1780
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• 2003

A continuous flow system has been developed to determine trace Cu(II), Mn(II), Ni(II) and Zn(II) in a large volume of water samples by a solvent sublation technique. The mixed solution of 1,10-phenanthroline(phen) and thiocyanate ion was used as ligands for the formation of their ternary complexes. The continuous system was constructed in this laboratory with a peristaltic pump, a mini shaker, three mixing bottles and a flotation cell by connecting each part with a polyethylene tube. The flotation conditions such as the flow rate of sample solution and the injection rates of ligand, buffer and surfactant solutions have been investigated to obtain the best sublation efficiencies. Each solution flowed into the flotation cell through each polyethylene tube by the peristaltic pumps. The ternary complexes were floated and extracted into MIBK in a flotation cell of 2 L by bubbling a nitrogen gas. The absorbances of extracted analytes in MIBK were directly measured by graphite furnace-AAS. The concentrations of 1,10-phenanthroline and thiocyanate ion were $2.6\;{\times}\;10^{-3}$ M and $2.3\;{\times}\;10^{-2}$ M in the mixed solution, respectively. The pH of sample solution was adjusted to 5.0 with a buffer solution and 1%(m/v) sodium lauryl sulphate solution was added as a surfactant to support the effective flotation of the complexes. The $N_2$ gas was bubbled at 30 mL/min for 90 minutes for 20 L of sample. Reproducible results of less than 10% RSD and recoveries of 80-120% could be obtained in real samples.

• KSBB Journal
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• v.10 no.3
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• pp.283-291
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• 1995

The characteristics of aminopeptidase M(ApM) immobilized covalently on Cellufine Formyl and the continuous production of authentic human growth hormone(hGH) from methionyl human growth hormono(met-hGH) using the column reactor packed with immobilized ApM were investigated. Immobilized ApM with the proportion of 2.3mg ApM per 1g Cellufine Formyl gel had the highest met-hGH conversion activity. The optimum pH(7.0) and temperature($55^{\circ}C$) showed no appreciable difference between free and immobilized enzymes and the optimum temperature in continuous operation of the column reactor was also found to be $55^{\circ}C$. Under the conditions at which met-hGH was converted completely to hGH, the yield and productivity were about 77% and 0.8mg hGH/ml$.$h, respectively. In two column reactors of different sizes, met-hGH was converted to hGH with the same conversion rates and hGH yields at the same space velocities. The half-life of the reactor systems at $45^{\circ}C$ and $55^{\circ}C$ were projected from the continuous operations for 90 days to be 225 days and 81 days, respectively.

• Journal of the Korean Society for Precision Engineering
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• v.13 no.4
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• pp.136-142
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• 1996

Automatic monitoring of cutting process is one of the most important technology in machining. AE sensing technology has been applied to monitoring process and proved to be effective in detecting tool abnor- malities such as tool wear and fracture. In this experimental study. AE signals were detected from the tool holder for continuous and interrupted cutting, which obtained from changing workpice material configuration, under control of constant cutting speed from CNC lathe. From statistical and frequency analysis, the AE signals were analyzed to obtaining the characteristics of continuous and interrupted cutting conditions and tool failure. The Kurtosis values decreased but RMS voltages increased as the cutting speed increased, in both continuous and interrupted cutting. RMS voltage is suddenly increased but Kurtosis value is suddenly decreased when tool failure condition. Power spectrum density of AE signals when tool failure reaches extreme value around 0.065 cycles/ .mu. m.

• Structural Engineering and Mechanics
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• v.55 no.6
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• pp.1099-1120
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• 2015

External prestressing has been applied to both new construction and retrofitting of existing reinforced and prestressed concrete structures. Continuous beams are preferred to simply supported beams because of economy, fewer movement joints and possible benefits from moment redistribution. However, this paper argues that continuous prestressed concrete beams with external unbonded tendons demonstrate different full-range behaviour compared to reinforced concrete (RC) beams. Applying the same design approach for RC to external prestressing may lead to design with a lower safety margin. To better understand the behaviour of continuous prestressed concrete beams with unbonded tendons, an experimental investigation is performed in which nine such specimens are tested to failure. The full-range behaviour is investigated with reference to moment-curvature relationship and moment redistribution. The amounts of moment redistribution measured in the experiments are compared with those allowed by BS 8110, EC2 and ACI 318. Design equations are also proposed to estimate the curvature ductility index of unbonded prestressed concrete beams.

• Proceedings of the Korea Information Processing Society Conference
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• 2008.05a
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• pp.718-720
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• 2008

In this paper, the study focus on continuous query in EPC Information Services(EPCIS) middleware which is a component of RFID system. We can consider EPCIS as a data stream system with a repository. In our work continuous query is implemented in two query execution model. One is standing query model another is traditional query execution model in which continuous query run over database periodically. Furthermore a balance strategy is presented. It is used to determine which continuous query implementation model is suitable for the query. Finally we conclude our work and issue some research topic for future work.

• Journal of the Korean GEO-environmental Society
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• v.7 no.4
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• pp.63-71
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• 2006

This study was conducted to evaluate air injection mode on stabilization of landfill gas and to predict the time for landfill mining. It took 8 times longer for pulse aeration to get to aerobic condition, compared to continuous aeration. It was evaluated that continuous aeration mode is more preferable than pulse mode for rapid air exchange in landfill mining. High correlation ($r^2$ = 0.95) was found between continuous aeration time and time to maintain aerobic condition when $0.2m^3/min$ of air was continuously injected and stopped. The aerobic condition ($CH_4$ < 5%) was maintained for 1.5 times longer than aeration time.

• Transactions of Materials Processing
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• v.16 no.1 s.91
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• pp.42-47
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• 2007

LCD-BLU is one of kernel parts of LCD and it consists of several optical sheets: LGP, light source and mold frame. The LGP of LCD-BLU is usually manufactured by etching process and forming numerous dots with $50\sim300{\mu}m$ diameter on the surface. But the surface of the etched dots of LGP is very rough due to the characteristics of the etching process during the mold fabrication, so that its light loss is high along with the dispersion of light into the surface. Accordingly, there is a limit in raising the luminance of LCD-BLU. In order to overcome the limit of current etched dot patterned LGP, optical pattern with continuous microlens was designed using optical simulation CAE. Also, a mold with continuous micro-lens was fabricated by UV-LiGA reflow process and applied to 7 inch size of navigator LCD-BLU in the present study.

• Journal of Life Science
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• v.14 no.1
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• pp.167-173
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• 2004

The optimal conditions for the continuous production of $\gamma$-aminobutyric acid by immobilization of Lactobacillus brevis BH-21 using column type reactor were investigated. The optimal conditions of operation were 2.2 mm diameter bead of 3.0% sodium alginate at 10 mL/h of substrate feeding rate. Continuous production by immobililzed cells showed the highest productivity with replacement of fresh medium in every 48h for fourth fermentatoin cycle following the rendition of $\gamma$-aminobutyric acid productivity. A productivity of $\gamma$-aminobutyric acid could be obtained for 25 days by continuous column type reactor under optimal conditions.