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Functional characterization of domestic and foreign green tea cultivars at different harvest periods (채취시기가 다른 국내외 녹차잎의 기능성분 함량, 뇌세포 생존 및 대사 효소 활성 조절 효과 조사)

  • Lee, Bang-Hee;Jeon, Sae Hyun;Jeong, Hana;Choi, Jung;Kim, Young-Min;Yang, Kwang-Yeol;Nam, Seung-Hee
    • Korean Journal of Food Science and Technology
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    • v.52 no.5
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    • pp.427-434
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    • 2020
  • This study was performed to compare nutritional compounds and physiological functions of five domestic and imported green tea cultivars at three time points. The five cultivars were compared for theanine, γ-aminobutyric acid, and catechin content by LC-MS/MS and HPLC. Furthermore, the five tea cultivars were functionally characterized with respect to antioxidant activity, brain cell protective effect, and inhibitions of α-glucosidase and HMG-CoA reductase activities. Among green tea cultivars, Chamnok had the highest content of catechins (198 mg/g DW), theanine (11.89 mg/g DW), and tannin (23.6 mg/g DW). Considering functional properties, Chamnok treatment resulted in the maximum viability of brain cells and reduced the cortisol content of SH-SY5Y cells. The inhibition of α-glucosidase and HMG-CoA reductase was the strongest following Chamnok treatment (72.9% and 69.8%, respectively). These results indicate that Chamnok could be optimal for consumption or favorable processing owing to its high nutritional compounds, such as theanine and catechin, and remarkable brain cell protective effects.

Prevalence and risk factors of subclinical bovine mastitis in some dairy farms of Sylhet district of Bangladesh

  • Kahir, Md. Abdul;Islam, Md. Mazharul;Rahman, A.K.M. Anisur;Nahar, A.;Rahman, Md. Siddiqur;Son, Hee-Jong
    • Korean Journal of Veterinary Service
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    • v.31 no.4
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    • pp.497-504
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    • 2008
  • A cross-sectional study was undertaken to report prevalence and to identify risk factors of subclinical mastitis of dairy cattle in Sylhet district of Bangladesh. Among 325 dairy farms of the district 12 farms(3.7%) were selected conveniently for this study. All the dairy cows of the 12 farms were selected for sample collection. Fresh milk samples from each of the selected dairy cows were collected aseptically in separate sterilized test tube as RF, RH, LF and LH quarter of the udder. Rapid modified White Side Test(WST) was used to detect subclinical mastitis(SCM). Results of WST and data derived from filled in questionnaire were entered in Microsoft Excel 2003 and transferred to $STATA^{(R)}$, version 8.0/Intercooled(Stata Corporation, Texas, USA, 2003). The overall prevalence of SCM and its distribution in different categories of variables in cow and their exact binomial 95% confidence intervals were calculated in $STATA^{(R)}$. Simple bivariable associations among independent variables were investigated by $x^2$ test in $STATA^{(R)}$. Multiple logistic regression analysis with backward elimination method was used to identify risk factors of SCM. To identify significant variation in quarter SCM, linear regression analysis was performed after arcsine transformation of the data. The overall prevalence of SCM found in this study is 54%. Dairy cows with teat lesions had significantly increased SCM(OR=12342, P value=0.000, 95% CI=762, 199798) than others without teat lesions. The Holstein Friesian X Jersey X Sahiwal breed has significantly decreased(OR=0.18, p=0.03, 95% CI 0.04, 0.85) SCM than other breeds. The prevalence of SCM found in this study is in agreement with others. The injury in the teat increases the probability of getting infected with microbes and thereby mastitis. If the prevalence of teat lesion can be decreased the probability of subclinical mastitis will also be decreased. The negatively associated Holstein Friesian X Jersey X Sahiwall breed may help in planning mastitis control program if this finding can be validated by a more powerful case-control or cohort study design.

Child Nutrition Survey in Rural Health Project Areas (농촌보건사업지역(農村保健事業地域)의 아동영양(兒童營養) 실태조사(實態調査))

  • Park,, Myung-Yun;Jang, Young-Ja;Seo, Jung-Sook;Mo, Su-Mi
    • Journal of Nutrition and Health
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    • v.13 no.1
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    • pp.15-26
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    • 1980
  • The main purposes of the nutrition and clinical surveys were to provide baseline information on the nutritional status of pre-school children in rural health demonstration project areas of the Korea Health Development Institute (KHDI) for nutrition guidance services for the MCH target group. The survey covered a total of 222 pre-school children and 135 mothers in Okgu Gun, Cholla Pukto Province from August 10 to August 17, 1979. The survey results are summarized as follows: 1) Family Environment Seventy percent of the households had more than three children, and the mean family sire was 6. Sixty-nine percent of the mothers and 47% of the fathers of the surveyed households were educated at or below the primary school level. The majority, 70% of the mothers, were aged between 20 years and 35 years. 2) Anthropometric Measurements and Hemoglobin Value 4.5% of the children were lower than 80% weight for age of the Korean standard, and 5.4% were lower than 85% arm circumference for age of the Jelliffe's standard resectively, and those were suffering from protein-energy malnutrition. Angular stomatitis were observed on 66.2% of the subjects. Mean hemoglobin value was 11.1g/100m1, and 44.2% of the subjects were categorized as anaemia. 3) Food and Nutrient Intake of animal foods was very low, ranging from 2.9 to 17%. Consumption of eggs was less than 2% of total food intake, and intake of legumes was also very scanty, between 0.8 to 3.7%. These data present evidence of very poor protein intake, quality as well as quantity. Energy intake of children was 60.0 to 64.4% of the recommended allowance, and mean protein intake only met 47.4% of the recommendation. Low intake of vitamins except thiamin were also found. 4) Mother's Nutrition Knowledge Eighty-five percent of the mothers were entirely ignorant regarding the 'five basic food group' which is most important fact on food and nutrition guidance. Mean knowledge score from 14 basic questions about food and nutrition was as low as 5.1. There was a significant positive correlation between mother's educational level and nutrition knowledge score. 5) Family Planning Variable There were significant correlation among maternal, family planning variables, and some of the nutritional and physical measurements. The study revealed that the mother's educational level and nutrition knowledge score are more crucial factors than the family planning variables on effecting food intakes on children. Recommendation : According to the results of the surveys, there were high incident rates of nutritional anaemia and angular stomatitis among pre-school children, and most of rural women had very limited knowledge about food and nutrition. As a main part of the health education activities, the community health workers should provide nutrition education to the village mothers to improve the nutrional status of young children in rural areas. Nutrional promotion at the primary health care level should be mainly based on appropriate nutrition education.

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Sterilizing Effect of Plant Pathogenic Fungi using Ozone Microbubble (오존마이크로버블을 이용한 식물병원균 살균효과)

  • Kim, Chang Shoo;Yu, Sang Yeol;Lee, Gong In;Kim, Seung Han;Lee, Jong Won;Song, Jae Kwan
    • Journal of Bio-Environment Control
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    • v.23 no.3
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    • pp.250-255
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    • 2014
  • Sterilization is an important factor in reprocessing of drained nutrient solution (RDN). To evaluate the suitability of ozone microbubble for RDN in protected horticulture and plant factory, strong fungicidal activity of ozone and microbubble were applied. This experiment was taken advantage of ozonated water (OW) and ozone gas (OG). The Fusarium oxysporum (FO), Phytophthora capsici (PC) and Colletotrichum gloeosporioides (CG) were treated with OW 0.5, 1.0 and 2.0 ppm and OG $3.0g{\cdot}h^{-1}$ for 0, 30, 60, 120 and 180 sec. Results of this experiment can be summed up as follows : In the OW, FO was sterilized by 0.5 ppm in 60 sec and PC was sterilized by 2.0 ppm in 30 sec. In the OG, FO and PC both of them were sterilized in 180 sec. However, CG was not sterilized using OW and OG. Overall, ozone microbubble showed possibility of sterilization in RDN. However, CG is required to more ozone concentration and processing time.

Cultivation and characterization of commercial strain "Hambak" derived by di-mono crossing in Grifola frondosa (Di-mono 교잡에 의한 잎새버섯 품종 "함박"의 특성 및 재배)

  • Kong, Won-Sik;Yoo, Young-Bok;Jhune, Chang-Sung;You, Chang-Hyun;Cho, Yong-Hyun;Park, Young-Hak;Kim, Kwang-Ho
    • Journal of Mushroom
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    • v.5 no.1
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    • pp.1-6
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    • 2007
  • A new commercial strain "Hambak" of Grifola frondosa was developed by di-mono crossing between dikaryon of ASI 9031 and monokaryotic strain derived from ASI 9021. It can be cultivated in plastic bottle and bag filled with oak and poplar sawdust substrate which supplemented with 15% rice bran or 10% corn bran. Improvement of cultivation methods was required for shortage of mycelium incubation period and management of humidity in growing room. The optimum temperature of mycelial growth was $25^{\circ}C$ and that of fruiting body development was $15{\sim}18^{\circ}C$. The color of pileus surface was brown and fine pores, whitish to cream-colored, were underside. Primordia formation of Hambak was two days faster than that of Yipsae 1. The yield was $97g/850m{\ell}$ bottle, a bunch of fruiting body was bigger and the quality was better than Yipsae 1.

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Development of New Vitrification Method for Preimplantation Mouse Embryo

  • Ha, A-Na;Fakruzzaman, Md.;Lee, Kyeong-Lim;Wang, Erdan;Lee, Jae-Ik;Min, Chan-Sik;Kong, Il-Keun
    • Journal of Embryo Transfer
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    • v.28 no.2
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    • pp.141-147
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    • 2013
  • The purpose of this study was attempted to new methods in mammalian embryos vitrification. This method was affected to increase of the embryo vitrification efficiency and it would be applied to the field of embryo transfer to recipient by modified loading method of embryo into 0.25 ml plastic straw. The frozen mouse embryos were carried out warmed from two different cell stages (8-cell and blastocyst, respectively) by attachment of an embryo in the vitrification straw (aV) method. All groups were cultured in M-16 medium to determine the development and survivability for 24 h, respectively. Results shown that, the survivability of two different groups were significantly different (94.8% vs. 70.9%). Total cell number was not significantly different the non-frozen blastocyst ($99.7{\pm}12.4$) compared to the post-thaw blastocyst ($94.8{\pm}15.1$). From the 8-cell embryo, total cell number of frozen blastocysts were significantly lower than others groups ($74.7{\pm}14.6$, p<0.05). In the case of cell death analysis, the blastocysts from non-frozen and frozen-thawed 8-cell group were not different ($0.0{\pm}0.0$ vs. $1.9{\pm}3.1$, p>0.05). However, the apoptotic nuclei of blastocyst were significantly observed the frozen-thawed group ($5.4{\pm}4.4$) compared to non-frozen group (p<0.05). Therefore, this new method of embryos using in-straw dilution and direct transfer into other species would be more simple procedure of embryo transfer rather than step-wise dilution method and cryopreservation vessels, so we can be applied in animal as well as human embryo cryopreservation in further.

A Study on the Establishment of a Fee System for Hospital based Home Nursing Care (일 종합병원에서의 가정간호수가 체계 방안을 위한 일 연구)

  • Kim, Cho-Ja;Jo, Won-Jeong;Choe, Hae-Seon
    • The Korean Nurse
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    • v.32 no.1
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    • pp.61-76
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    • 1993
  • This study was done in order La provide basic data to a Fee System for hospital based Home Health Care services in Korea in the future. It was done by investigating activities provided to possible Home Health Care clients who could be discharged early from genera] hospitals and then estimating the nursing care fee according to each nursing activity based upon the time used for activity. The subjects of the study were selected by convenience sampling and consisted of 35 clients who might be clients for Home Nursing Care and were presently admitted to a medical- surgical ward of Y University Medical Center located in Seoul, Korea. The data collection period was from September 1, 1991 to September 30, 1991. The research in strum nets utilized for the study were a client selection criterial for Home Health Care developed by Choo(l991) and a check-list of nursing activity developed by researcher. The results of the study were as follows : 1. There were 44 different nursing activities provided in the seven days but the time was calculated for only 25 of the nursing activities. 2. Fees for the 25 different nursing activities were calculated by multipling the median of the average wage of a staff nurse having five years experience in an A grade general hospital to the Lime of the nursing activity. The results were compared with the insurance fee which the government recognized as an appropriate fee for that activity. The nursing activities with a lower calculated fee than the insurance fee were suction, catheterization, exercise education and dressing change. The nursing activities with a higher calculated fee than the government recognized fee were 1M injection and vital sign check. 3. There was a range of 1-15 nursing activities provided daily to the client. For the average number of nursing activities per day of 6.26 events the nursing care fee was calaulated at W 6136 per day. 4. Based upon the results of the study, a recommentdation for a Home Health Care fee per visit based on the nursing activities provided could be formulated for a Home Health Care fee system. It could be formulated as following: 1) Home health Care fee per visit $=[(direct{\;} nursing{\;}fee(direct{\;}nursing{\;}care{\;}time{\;}per{\;}activity{\;}{\times}{\;}average{\;}nursing{\;}wage)+indirect fee]{\times}average$ nursing activity per visit]+management fee+ materials fee+a travel fee In this way a nursing fee could be calculated based upon the result of the study of the nursing fees per visit. 2) Nursing activity fees per visit. = $([direct nursing{\;}care{\;}fee+indirect{\;}nursing{\;}fee]{\times}average$ number of nursing activities provided per visit] (W 6, 136) + travel fee(\ 5, 542) +management fee material $fee({\alpha})\{\;}16, 436+{\alpha}$ The nursing fee per visit as calculated in this research of $\{\;}15, 0000+{\alpha}$ could be adjusted according to the patient's condition or the use of high technology nursing care or according to the amount of time spent for travel. The nursing care fee per visit presented in this study can be validated through a Home Health Care demonstration project.

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Evaluation of trace mineral source and preharvest deletion of trace minerals from finishing diets on tissue mineral status in pigs

  • Ma, Y.L.;Lindemann, M.D.;Webb, S.F.;Rentfrow, G.
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.2
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    • pp.252-262
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    • 2018
  • Objective: An experiment was conducted to evaluate dietary supplemental trace mineral source and deletion on mineral content in tissues. Methods: Weanling crossbred pigs (n = 144; 72 barrows and 72 gilts; body weight [BW] = $7.4{\pm}1.05kg$) were used. A basal diet was prepared, and trace mineral premix containing either inorganic (ITM) or organic (OTM) trace minerals (Cu, Fe, Mn, and Zn) was added to the basal diet. Pigs were blocked by sex and BW and randomly allotted to 24 pens for a total of 6 pigs per pen, and fed a diet containing either ITM or OTM supplemented at the 1998 NRC requirement estimates for each of 5 BW phases (Phase I to V) from 7 to 120 kg. The trace mineral supplementation was deleted for 6, 4, 2, and 0 wk of Phase V; regarding nutrient adequacy during this phase, the indigenous dietary Fe and Mn was sufficient, Cu was marginal and Zn was deficient. Results: At the end of Phase IV, Mn content (mg/kg on the dry matter basis) was greater (p<0.05) in heart (0.77 vs 0.68), kidney (6.32 vs 5.87), liver (9.46 vs 8.30), and longissimus dorsi (LD; 0.30 vs 0.23) of pigs fed OTM. The pigs fed OTM were greater (p<0.05) in LD Cu (2.12 vs 1.89) and Fe (21.75 vs 19.40) and metacarpal bone Zn (141.86 vs 130.05). At the end of Phase V, increased length of deletion period (from 0 to 6 wk) resulted in a decrease (linear, p<0.01) in liver Zn (196.5 to 121.8), metacarpal bone Zn (146.6 to 86.2) and an increase (linear, p<0.01) in heart Mn (0.70 to 1.08), liver Mn (7.74 to 12.96), and kidney Mn (5.58 to 7.56). The only mineral source by deletion period interaction (p<0.05) was observed in LD Zn. Conclusion: The results demonstrated differential effects of mineral deletion on tissue mineral content depending on both mineral assessed and source of the mineral.

"I'm healthy, I don't have pain"- health screening participation and its association with chronic pain in a low socioeconomic status Singaporean population

  • Wee, Liang En;Sin, David;Cher, Wen Qi;Li, Zong Chen;Tsang, Tammy;Shibli, Sabina;Koh, Gerald
    • The Korean Journal of Pain
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    • v.30 no.1
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    • pp.34-43
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    • 2017
  • Background: We sought to determine the association between chronic pain and participating in routine health screening in a low socioeconomic-status (SES) rental-flat community in Singapore. In Singapore, ${\geq}85%$ own homes; public rental flats are reserved for those with low-income. Methods: Chronic pain was defined as pain ${\geq}3$ months. From 2009-2014, residents aged 40-60 years in five public rental-flat enclaves were surveyed for chronic pain; participation in health screening was also measured. We compared them to residents staying in adjacent owner-occupied public housing. We also conducted a qualitative study to better understand the relationship between chronic pain and health screening participation amongst residents in these low-SES enclaves. Results: In the rental-flat population, chronic pain was associated with higher participation in screening for diabetes (aOR = 2.11, CI = 1.36-3.27, P < 0.001), dyslipidemia (aOR = 2.06, CI = 1.25-3.39, P = 0.005), colorectal cancer (aOR = 2.28, CI = 1.18-4.40, P = 0.014), cervical cancer (aOR = 2.65, CI = 1.34-5.23, P = 0.005) and breast cancer (aOR = 3.52, CI = 1.94-6.41, P < 0.001); this association was not present in the owner-occupied population. Three main themes emerged from our qualitative analysis of the link between chronic pain and screening participation: pain as an association of "major illness"; screening as a search for answers to pain; and labelling pain as an end in itself. Conclusions: Chronic pain was associated with higher cardiovascular and cancer screening participation in the low-SES population. In low-SES populations with limited access to pain management services, chronic pain issues may surface during routine health screening.

브래드포드법칙과 그 응용에 관한 고찰

  • 이경호
    • Journal of Korean Library and Information Science Society
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    • v.8
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    • pp.127-154
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    • 1981
  • Today's complicated society with a rapid change needs more objective and accurate data for the better managerical decisions and the prospect for the future other than the intuition or subjective experience by men themselves. These data can't be extracted without the analysis of actual data by a n.0, pplying any of mathematical techniques. One of these mathematical techniques, called bibliometrics has been newly developed in the field of library and information science to extract the objective data for the better services through the library operations. The Beadford's Law, one important law in bibliometrics has provided rather scientific and objective basis on the more valid building of library collection within the constraints of budget. The purpose of the study is to investigate the theory of the Bradford's Law, to clarify the possible areas of its a n.0, pplication, and to discern some problems in doing so. The results of the study can be summarized as follows; (1) There is certain difference between the graphical formulation and verbal formulation of Bradford's Law. But this law is very useful for the field of library and information science, owing to the flexibility of the a n.0, pplication of the law in the field. (2) The minimal nucleus can consist of a single periodical only if j, the number of relevant papers in the most productive journals is greater than Z/2. On the other hand, if j is less than or equal to Z/2, then the minimal nucleus will consist of 2 or more periodicals. (3) It is possible to design the most compact selection of scientific periodicals covering any specified percentage P among the total periodicals by using the formulation, log n=Plog N+(1-P)logs, or Nlog N/s=P center dot N log N/s. (4) If there is need to provide all the articles needed by users the given budget, the library can purchase the proper number of journals, by using the formulation, f center dot AN=An+PN (log N/s-logn/s). (5) In the building of the library collection based on the decreasing ratio of use, the library can subscribe to the proper number of journals according to the satisfactory degree of the need, by using the formulation, f=Nu+uNlogN/S-uNlogn/s / nNlogN/s = 1+logN/m /logN/s (6) If the order of valuable journals is decided according to the frequency of being cited, the order can't always represent the value. (7) The evaluation criteria for the journals with high value, but less cited should be made a further study.

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