Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Development of New Vitrification Method for Preimplantation Mouse Embryo

  • Ha, A-Na (Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University) ;
  • Fakruzzaman, Md. (Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University) ;
  • Lee, Kyeong-Lim (Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University) ;
  • Wang, Erdan (Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University) ;
  • Lee, Jae-Ik (Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University) ;
  • Min, Chan-Sik (Gyeongsangnamdo Agriculture Research & Extension Services) ;
  • Kong, Il-Keun (Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University)
  • Received : 2013.04.29
  • Accepted : 2013.05.24
  • Published : 2013.06.30
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Abstract

The purpose of this study was attempted to new methods in mammalian embryos vitrification. This method was affected to increase of the embryo vitrification efficiency and it would be applied to the field of embryo transfer to recipient by modified loading method of embryo into 0.25 ml plastic straw. The frozen mouse embryos were carried out warmed from two different cell stages (8-cell and blastocyst, respectively) by attachment of an embryo in the vitrification straw (aV) method. All groups were cultured in M-16 medium to determine the development and survivability for 24 h, respectively. Results shown that, the survivability of two different groups were significantly different (94.8% vs. 70.9%). Total cell number was not significantly different the non-frozen blastocyst ($99.7{\pm}12.4$) compared to the post-thaw blastocyst ($94.8{\pm}15.1$). From the 8-cell embryo, total cell number of frozen blastocysts were significantly lower than others groups ($74.7{\pm}14.6$, p<0.05). In the case of cell death analysis, the blastocysts from non-frozen and frozen-thawed 8-cell group were not different ($0.0{\pm}0.0$ vs. $1.9{\pm}3.1$, p>0.05). However, the apoptotic nuclei of blastocyst were significantly observed the frozen-thawed group ($5.4{\pm}4.4$) compared to non-frozen group (p<0.05). Therefore, this new method of embryos using in-straw dilution and direct transfer into other species would be more simple procedure of embryo transfer rather than step-wise dilution method and cryopreservation vessels, so we can be applied in animal as well as human embryo cryopreservation in further.

Keywords

References

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