• Title/Summary/Keyword: lycoris

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Methanolic Extract Isolated from Root of Lycoris aurea Inhibits Cancer Cell Growth and Endothelial Cell Tube Formation In Vitro

  • Kang, Moo-Rim;Lee, Chang-Woo;Yun, Ji-Eun;Oh, Soo-Jin;Park, Song-Kyu;Lee, Ki-Ho;Kim, Hwan-Mook;Han, Sang-Bae;Kim, Hyoung-Chin;Kang, Jong-Soon
    • Toxicological Research
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    • v.28 no.1
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    • pp.33-38
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    • 2012
  • In this study, we investigated the effect of methanolic extract isolated from the root of Lycoris aurea (LA) on the growth of cancer cells and the tube formation activity of endothelial cells. Various cancer cells were treated with LA at doses of 0.3, 1, 3, 10 or 30 ${\mu}g/ml$ and LA significantly suppressed the growth of several cancer cell lines, including ACHN, HCT-15, K-562, MCF-7, PC-3 and SK-OV-3, in a dose-dependent manner. We also found that LA induced cell cycle arrest at G2/M phase in ACHN renal cell adenocarcinoma cells. Further study demonstrated that LA concentration-dependently inhibited the tube formation, which is a widely used in vitro model of reorganization stage of angiogenesis, in human umbilical vein endothelial cells. Collectively, these results show that LA inhibits the growth of cancer cells and tube formation of endothelial cells and the growth-inhibitory effect of LA might be mediated, at least in part, by blocking cell cycle progression.

Isolation of Herbicidal Substances from Bulbs of Lycoris flavescens M.Y.Kim & S.T.Lee (붉노랑상사화 인경으로부터 살초활성 물질의 분리)

  • Lee, Dong-Gu;Kim, Kun-Woo
    • Korean Journal of Weed Science
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    • v.31 no.4
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    • pp.330-339
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    • 2011
  • This study was conducted to determine the herbicidal activity of herbicidal substances and identify them in bulbs of Lycoris flavescens. Methanol extract was purified by a series of chromatographic techniques including silica gel column chromatography, preparative TLC, and HPLC. The final HPLC gave two active fractions and two herbicidal substances were obtained. By GC/MS analysis, one was identified as galanthine (galanthan-1-ol) and the other was identified as montanine ($O^2$-methyl pancracine), an isoquinoline alkaloid. Montanine showed nearly 100% of growth inhibition on the shoot and root of barnyardgrass (Echinochloa crusgalli) seedlings at $20{\mu}g\;mL^{-1}$ as compared with the control. Meanwhile, methanol extract of L. flavescens bulbs showed only about 3.1% and 8.3% of growth inhibition on the shoot and root of rice cultivar, Hwayeongbyeo seedlings at $1,000{\mu}g\;mL^{-1}$ as compared with the control, respectively.

Effective and Economical Propagation Method of Lycoris squamigera Native to Korea (한국산 자생 상사화(Lycoris squamigera MAX.)의 효과적인 번식방법)

  • Park, Yun Jum;Heo, Buk Gu;Jeong, So Young;Jeong, Jai Ho;An, Min Sil
    • Horticultural Science & Technology
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    • v.16 no.2
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    • pp.242-243
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    • 1998
  • This research was conducted to find the most effective and economical artificial propagation method of Lycoris squamigera among chipping, half-chipping, twin-scaling, coring, scooping and notching, in order to investigate the number of bulblets obtained from one bulb and the number of bulbs treated by a man in a day for artificial propagation. We got 42.2 bulblets from one bulb by twin-scaling, 23.2 bulblets by half-chipping, 18.2 bulblets by chipping, 14.3 bulblets by notching, 1 bulblet by coring and also 1 bulblet by scooping. Although we got the most bulblets by twin-scaling, it took the most time to treat the bulbs for artificial propagation (200 bulbs per day) and the bulblets formed were very small. But 400 bulbs were treated in a day by chipping and the bulblets formed were comparatively large. In addition, machines could be used for chipping, so chipping was considered to be the most effective and economical artificial propagation method.

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Plant Regeneration from Leaf and Root Cultures of Lycoris chejuensis via Bulblet Formation (제주상사화 (Lycoris chejuensis K. Tae et S. Ko) 잎 및 뿌리 절편으로부터 소자구 형성을 통한 식물체 재생안)

  • Oh, Myung-Jin;Park, Jong-Mi;Tae, Kyoung-Hwan;Liu, Jang-Ryol;Kim, Suk-Weon
    • Journal of Plant Biotechnology
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    • v.34 no.3
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    • pp.223-227
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    • 2007
  • Plant regeneration system from leaf and root segments of Lycoris chejuensis via bulblet formation was established. Surface-sterilized leaf and root segments were cultured on the B5 medium containing 2,4-D. After 12 weeks of culture onto B5 medium containing 2,4-D, white globular structures and white calluses were formed on the cut surface of the explants. The highest frequency of globular structures and calluses formation from leaf explants was 32.1% when leaf explants were cultured onto B5 medium supplemented with 1 mg/L of 2,4-D. However, the higher concentration of 2,4-D (over than 3 mg/L) resulted in decrease of the frequency. In comparison to leaf explants, root segments showed the highest frequency at a rate of 36.1% when root explants were cultured onto B5 medium supplemented with 3 mg/L of 2,4-D. These structures and calluses were sub-cultured and proliferated onto the same culture medium. Upon transfer to B5 basal medium, white globular structures were developed into bulblets and normal plantlets. After 4 weeks of incubation in the light, plantlets were successfully rooted over the frequency of approximately 90%. Rooted plantlets were successfully transferred to potting soil and acclimatized in the growth chamber. The plant regeneration system of Lycoris chejuensis established in this study, might be applied to mass proliferation, conservation of genetic resources and genetic transformation for molecular breeding.

A Herbological Study on the Plants of Amaryllidaceae in Korea (한국산 수선화과 식물에 관한 본초학적 연구)

  • Jeong, Jong-Gil
    • The Korea Journal of Herbology
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    • v.31 no.1
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    • pp.41-47
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    • 2016
  • Objectives : For the purpose of developing Korean herbology of the plants to Amaryllidaceae in Korea, the literatures of the successive generations have been thoroughly investigated to prepare this article.Methods : Books and research papers about Herbology which published at home and abroad were examined.Results : A list was made about Amarylliaceae plant which were cultivated or grew naturally in Korea, after investigated the data on domestic and foreign Amarylliaceae plants. Out of those lists, serviceable plants were selected and with those available plants, their distributions were analysed and parts which can be used as medicines were divided into 3 categories such as oriental medicine name, scientific name and non-official name. Amarylliaceae's properties flavor, channels they use, effects, and toxicity were also noted. Amarylliaceae (grew in Korea) were divided into 4 classes with 16 species. Out of those, 4 classes with 7 species were found serviceable which indicates 44 % of all. Out of all 16 specified Amarylliaceae plants, Lycoris plants were found 9 species, which were shown the most. And 4 classes of Lycoris plants were also selected the most in serviceable Amarylliaceae. Out of all serviceable parts in Amarylliaceae, bulbus parts took first place as 6 species.Conclusions : There were totaled to 4 genera and 16 species in Amarylliaceae in Korea and among them medicinal plants are 4 genera, 7 species, some 44 % in total.

The Specific Plant Species and Naturalized Plants in the Area of Naejangsan National Park, Korea (내장산국립공원 일대의 특정식물과 귀화식물)

  • Lee, Hee-Cheon;CheKar, Eun-Key;Lim, Dong-Ok
    • Korean Journal of Environment and Ecology
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    • v.25 no.3
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    • pp.267-283
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    • 2011
  • Endangered species designated by The Ministry of Environment in region of Naejangsan National Park were found Lycoris chinensis var. sinuolata K.H.Tae & S.T.Ko, Iris koreana Nakai, Cymbidium macrorrhizum Lindl and Vexillabium yakushimensis (Yamam.) F.Maek.(4 taxa). The floristic special plants were recorded to a total of 159 taxa; that is, class I species (91 taxa) were containing as Chloranthus fortunei (A.Gray) Solms, Vicia anguste-pinnata Nakai and Euscaphis japonica (Thunb.) Kanitz, etc, class II species (17 taxa) were Pseudostellaria coreana (Nakai) Ohwi, Dryopteris expansa (C.Presl) Fraser-Jenkins et Jermy and Gymnocarpium dryopteris (L.) Newman, etc, class III species (32 taxa) were containing; Cirsium setidens (Dunn) Nakai, Parasenecio pseudotaimingasa (Nakai) B.U.Oh and Saussurea eriophylla Nakai, etc, class IV species (9 taxa) were Deutzia paniculata Nakai, Carex ligulata Nees and Thuja orientalis L., etc, and class V species (10 taxon) were Orobanche filicicola Nakai, Lycoris chinensis var. sinuolata K.H.Tae & S.T.Ko and Lycoris sanguinea var. koreana (Nakai) T.Koyama, etc. Endemic species of korea were identified 40 taxa such as Broussonetia kazinoki var. humilis Uyeki, Pseudostellaria coreana (Nakai) Ohwi and Silene seoulensis Nakai, etc. Plant species designated as Natural Monument were 2 species that Daphniphyllum macropodum forest(No. 91) and Torreya nucifera forest(No. 153) receive protection. The naturalized plants were identified 30 taxa; Fallopia dumetorum (L.) Holub, Persicaria orientalis (L.) Spach and Rumex crispus L., etc. Among them the ecosystem disturbance wild plants was not discovered.