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Factors Regulating the Nitrogen Fixation Activity and Growth of Anabaena variabilis ATCC 29413 (Anabaena variabilis ATCC 29413 의 생장과 질소고정활성의 조절요인)

  • 송승달;한동훈
    • Korean Journal of Microbiology
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    • v.30 no.5
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    • pp.391-396
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    • 1992
  • Anabaena variabilis A TCC 29413. a photoautotrophic and nitrogen fixing cyanobacteria. was investigated on the environmental factors regulating the growth and nitrogen lixation activity. A good growth of cyanobacteria] cells was observed due to nitrogen t1xation by the heterocyst differentiation in nitrogen free Allen and Arnon (]/8) medium. The nitrogenase activity was appeared to be in proportion to the cell growth lor 6 days then drastically decreased in the later growth period when the nitraTe was accumulated to high level in the culture to cause the inhibition. The optima] conditions lilr the cell growth and nitrogenase activity of A. varillbili.l were anaerobic. IO.OO0 lux. $30^{\circ}C$ and pH 8 with the nitrogen Cree minimal medium. The activity was significantly inhihited by the low concentrations of ammonium and nitrate. but was stimulated b) the ]ow Ieve] of phosphate and carbonate sources. The treatments of several toxic heavy metals showed strong inhibition of the cell growth and nitrogenase activity by O.3~10 ppm in the order of $Hg^{2+}$ > $Cd^{2+}$ > $Co^{2+}$ > $Zn^{2+}$ > $Ph^{2+}$, and the concentrations for 50% inhibition of the maximum activity were 0.41. 0.47. 0.5 L 0.66 and 8.1 ppm. respectively. The addition of carbohydrates (0.5~ 1.0%) in the dark condition stimulated the growth and activity in the order of sucrose > fructose > glucose.

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Evaluation of Six Species Ciliates as a Live Food and Culture Environment for Euplotes sp. (먹이생물로써의 섬모충 6종의 평가와 Euplotes sp.의 배양 환경)

  • Yoo Jin Hyung;Hur Sung Bum
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.4
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    • pp.342-347
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    • 2002
  • Ciliates have the possibility of a new live food in marine finfish culture because of their wide range of body size, thin tell wall, show motility, and fast reproduction rate. In this research, six species of ciliates were isolated from south coast and salt pond in Korea. The fitness of these species as a live food was evaluated in terms of size, motility, suspensibility and cell density. As the result, Euplotes sp. (K-1) was found suitable to be a new live food which might substitute rotifers, Brachionus plintilis and B. rotundiformis in fish larvae culture. The modified $F{\emptyset}yn's$ Erdschreiberd media, MErds-2 with the addition of glycine, glucose and yeast extract increased six times higher growth rate of Euplotes sp. (K-1) than the basic F$\emptyset$yn's Erdschreiberd media. The optimum water temperature, pH and light intensity for this ciliates were $22.5^{\circ}C$, 8 and 2,000 lux, respectively, and its culture environmental range was relatively wide, On the other hand, this ciliate fed baker's yeast, Saccharomyces cererisiae grew up to 1,240 inds./mL with the inocula of 100 inds./mL within 7 days. The results of the study showed that Euplotes sp. (K-1) has a potential to be utilized as a new live food in fish larvae culture.

The Status and Prospect of Poplar Research in Korea (포플러 연구현황과 전망)

  • 구영본;여진기
    • Journal of Korea Foresty Energy
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    • v.22 no.2
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    • pp.1-17
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    • 2003
  • Populus species have been as a model species in tree breeding and we have enormous varieties resulting from the poplar breeding because of their fast growth performance and short rotation age. New varieties developed in Korea are common italian poplar(P euramericana, I-214, I-476), P euramericana“Eco 28”(Italian poplar No.1) and p. deltoides“Lux”(Italian poplar No.2), which were introduced from foreign countries. As hybrid polars, Hyun-Sasi(p. alba ${\times}$ P. glandulosa No.1, No.2, No.3, No4.), P. nigra x P. maximowiczii and P. koreana x P. nigra val. italica, were developed, and P. davidiana was selected as the result of selection breeding The total plantation areas covered with the new varieties are 935,162ha that include 745,773ha of P. euramericana, 184,636ha of P. alba x P. glandulosa, and other new varieties are 4,735ha. The new poplars are contributed to increase farmer's income as well as bare land tree-planting in Korea. The technologies associated with the poplar species were developed, such as the determination of optimum site for new the poplar species, the crossing method between incompatible poplar species, and the vegetative mass propagation. In the future, poplar species will be considered for phytoremediation species at contaminated areas such as landfill sites or with lives stock's waste water as well as wood production, a shade tree like road-side tree and public park tree.

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Effects of Dietary Vitamin E and Selenium Supplementation on Meat Color Stability of Hanwoo(Korean Native Cattle) Bull Beef during Retail Display (Vitamin E와 Selenium의 급여가 비거세우육의 진열저장중 육색 안정성에 미치는 영향)

  • 김용선;양성운;김주용;박연수;황환섭
    • Food Science of Animal Resources
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    • v.22 no.2
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    • pp.108-114
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    • 2002
  • The effects of dietary vitamin E and selenium(Se) supplementation on meat color stability in M. Longissimus of Hanwoo(Korean native cattle) bull beef during retail display(5$^{\circ}C$, 1,200 lux) were investigated. Experimental groups were divided into control(Vit E 27 IU/head/day, Se 0.09 mg/head/day), Vit E (2,500 IU/head/day), Se(20 mg/head/day), Vit E+Se(Vit E 2,500 IU/head/day, Se 20 mg/head/day) groups. CIE a*(redness), chroma(C*) values, oxymyoglobin(%) and R630-R580 were significantly (p<0.05) decreased among the 4 treatment groups during retail display, in particular, those values decreased more rapidly in the control group. The metmyoglobin (%) of 0 day(before storage) was not significantly (p<0.05) different among the 4 treatment groups. However, the rate of metmyoglobin accumulation during storage increased more rapidly in the control group. Therefore, discoloration in the control group was more accelerated compared to the other groups. TBARS(thiobarbituric acid reactive substances) which represent lipid rancidity was significantly(p<0.05) lower in Se and Vit E+Se groups than in the control and Vit E groups. Reducing ability of 0 day(before storage) was significantly lower in the control group than in the other groups, and it decreased more rapidly in the control group after 3 days of storage. Consequently, Se-supplemented groups(Se and Vit E+Se groups) were more resistant to lipid oxidation than were the control and Vit E groups. The stability of meat color and myoglobin forms(%) were significantly (p<0.05) higher in Vit E, Se and Vit E+Se groups than in the control group, but there were no difference among Vit E, Se and Vit E+Se groups.

Lipid Oxidation and Color Stability of Korean Native Black Pork and Modern Genotype Pork under Light-Exposure during Refrigerated Display (진열중 조도 처리에 따른 재래종 돈육과 개량종 돈육의 지방산화 및 육색 안정성 비교)

  • Kang, Sun-Moon;Lee, Sung-Ki
    • Food Science and Preservation
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    • v.14 no.4
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    • pp.400-407
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    • 2007
  • This study compared lipid oxidation and color stability of M. longissimus from five Korean native black barrows (66 kg average weight; KNP) and modern genotype barrows ($Landrace{\times}Yorkshire{\times}Duroc$, 112 kg average weight, MGP) during light exposure in a refrigerated shop display. The meat samples were aged at $1^{\circ}C$ for 5 days and either kept in the dark, or under light of flux 3,000 lux, at $4^{\circ}C$ for 7 days. The KNPs contained larger amounts of fat than did MGPs (p<0.05), but KNPs had lower $C_{14:0},\;Cl_{8:3},\;and\;C_{20:5}$ contents than did MGPs (p<0.05). The pH of KNP was lower than that of MGP, and increased more rapidly upon exposure to light. The TBARS assay showed that oxidized lipid levels increased more rapidly in KNP than in MGP, and this accumulation was accelerated by exposure to light. The KNP was always a darker red in color than was MGP, and the color deteriorated more rapidly in the light than in the dark. The sensory attributes of raw pork were reduced by exposure to light, but KNP showed higher sensory attributes than did MGP. Compared to the MGP, the KNP showed greater color stability under light, retained desired sensory attributes under light longer, but showed a lower lipid oxidation stability under light. Overall, light exposure accelerated lipid oxidation in and discoloration of pork.

A study on the management of harmful working environments for Increase of Labor productivity. (노동생산성 향상을 위한 유해작업환경관리에 관한 연구)

  • 조태웅;유익현;박성애
    • Journal of Environmental Health Sciences
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    • v.3 no.1
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    • pp.27-44
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    • 1976
  • This study was carried out to evaluate the harmful factors in working environments and to investigate the labor productivity after improvement of environments, surveying 93 industrial establishments of 10 industries located in Youngdeungpo industrial area in Seoul. The results obtained were as follows: 1) The highest noise level of 125dB(A) was indicated at the rolling process of transport equipment manufacturing industry. 2) The best illumination level was shown in precise machinery industry and the worst was indicated in rubber products, metallic products and transport equipment manufacturing industries. 3) Thermal conditions were above threshold limit value (TLV) at more than two processes of all industries except printing industry. 4) The highest dust concentration was determined in textile and wearing manufacturing industry. 5) Organic solvents were detected at 52 processes in 93 industrial establishments and 33 processes of them showed higher than TLV. The results about harmful chemicals were as follows: a) sulfur dioxide ($SO_2$)was determined higher than TLV on welding process of metallic product manufacturing industry and heat treatment process of transport equipment manufacturing industry. b) Carbon monoxide (CO) concentration was 700ppm at heat treatment process of transport equipment manufacturing industry, indicating 14 times of TLV. c) vinylchloride concentration in the air of PVC raw material mixing process and PVC preparation process of chemical product manufacturing industry was determined higher than TLV. d) Hydrochloride (HCl) concentration in the air of wire expanding process of transport equipment manufacturing industry was determined higher than TLV. 7) Higher values of lead concentration than TLV were determined at lead welding metallic product manufacturing industry and type planting process of process of printing industry, $1.8mg/m^3$ and $0.3mg/m^3$ respectively. 9) 22, 968 of 52, 855 workers (i.e. 43.5%) in 93 industries were exposed to various harmful agents. 10) It was found that the improvement of illumination in electric apparatus manufacturing industry (from 20~40 lux to 420 lux) resulted in an increase in productivity of 6.5% per capita and a decrease in faulty products of 19%. 11) Improvement of environments using local exhaust ventilation system resulted in a decrease of harmful substances lower than TLV and an increase in productivity of 11.4%. 12) Improvement of shovelling tools based on ergonomics resulted in a reduction in energy expenditure of 25.3% and an increase in productivity of 32.2% per capita.

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Growth and Yield Responses of Soybean under Night Illumination at Different Growth Stages (콩의 생육단계별 야간조명에 따른 생육 및 수량 반응)

  • 김충국;서종호;조현숙;김시주;허일봉
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.6
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    • pp.478-482
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    • 2001
  • This experiment conducted to know physiological characteristics and stress effect on different growth stage of soybean by night illumination. Soybean variety, Shinpaldalkong 2, Keumjungkong and Muhankong were treated by night illumination with 20~30 Lux (0.05~0.08W m$^{-2}$ , 0.24~0.36 $\mu$㏖ S$^{-1}$ m$^{-2}$ ) for 15 days at six different growth stage, seedling, pre-floral initiation, post-floral initiation, pod filling and seed ripening stage. Night illumination delayed flowering to 2~8 days compared to control. Delay of flowering by night illumination severely effected at the pre-floral initiation stage. Stem length was increased all the night illumination treatments except the pod filling stage. Number of nodes in Shinpaldalkong 2 and Keumjungkong 1 were increased until before post-floral initiation stage but in Muhankong were increased until after post-floral initiation stage by night illumination treatments. Number of pods were decreased all the night illumination treatments except seedling stage compared with control. Yield decreased all the treatments and severe the loss rate degree showed the order of prefloral initiation, post-floral initiation, seedling and flowering stage.

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Effect of Temperature and Nutrition Affecting Oospore Formation of Phytophthora capsici Causing Red Pepper Fruit Rot (고추 역병균(疫病菌) (Phytophthora capsici)의 난포자(卵胞子) 형성(形成)에 미치는 온도(溫度) 및 영양(營養)의 효과(效果))

  • Chung, Bong-Koo;Kang, Me-Jong
    • The Korean Journal of Mycology
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    • v.18 no.4
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    • pp.203-208
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    • 1990
  • Sexual reproductive structure of Phytophthora capsici in vitro was round shape with thick wall and $24.4\;{\mu}m$ of diameter ranging $20-32.5\;{\mu}m$. Oogonium was $26.7\;{\mu}m$ $(21-37.5\;{\mu}m$) and $6.5-{\times}5.4m$ $(6-8{\times}5-6{\mu}m)$ for antheridium as doughnut shaped. Since mycelial contact of the paired cultures initiated right after inoculation, mycelial expansion phase was followed. Oospore morphogensis could be divided into the four phases for reproducing adult oospores. The optimum temperature for oospore reproduction was $20-24^{\circ}C$, whereas a retard trend for oospore formation was at the temperature above $25^{\circ}C$. Korean squash agar medium showed a higher oospore formation than the existing V-8 agar medium. Red pepper fruit agar medium was next. No oospore was reproduced on the red pepper leaf medium. Diurnal light with $1800{\pm}300$ and $800{\pm}300$ Lux showed rather retardation for oospore formation than dark conditions.

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Effect of Cultivation Method, Harvest Season and Preservative Solution on the Quality and Vase Life of Cut Rose 'Rote Rose' (롯데로제 장미의 재배방법, 수확시기 및 보존제 종류가 절화 품질과 수명에 미치는 영향)

  • Cho, Mee Sook;Hwang, Seung Jae;Jeong, Byoung Ryong
    • Horticultural Science & Technology
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    • v.19 no.1
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    • pp.71-77
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    • 2001
  • Experiments were conducted to evaluate quality and vase life of cut rose 'Rote Rose' cultivated in soil or hydroponically in rockwool. Rose flower stems harvested in commercial greenhouses in Kimhae on May 27 and June 14, 1998 were transported for about two hours to a laboratory and recut in water to an uniform stem length of 45cm. The rose flowers harvested on the same day were displayed at a density of $10cm{\times}10cm$ and were subjected to the same environmental conditions in a growth chamber. The stems were put in four different preservative solutions, 0.5% Chrysal RVB, BS (2% sucrose+200ppm 8HQS+0.3% Chrysal RVB), Sonk1 (BS+0.1mM ethionine), and double distilled $H_2O$. Flower stems harvested on May 27 were displayed at $18{\pm}1^{\circ}C$, RH 60-70%, and light intensity of 220lux provided by fluorescent lamps for $16h{\cdot}d^{-1}$. Flower stems harvested on June 14 were displayed at $25{\pm}1^{\circ}C$, RH 70-80%, and light intensity of 220lux provided by fluorescent lamps for $16h{\cdot}d^{-1}$. Fresh weight and flower diameter were affected by cultivation method, and were greater in hydroponically-grown roses than in soil-grown roses. Among the preservative solutions, BS and Sonk1 were superior to Chrysal RVB in terms of prolonging vase life. Vase life extension in Chrysal RVB, BS and Sonk1 over the control was about one day in both display temperatures. At $18^{\circ}C$ vase life was maintained for three to four additional days as compared to that at $25^{\circ}C$.

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Characteristics of Photosynthesis among New Cultivars of Ginseng (Panax ginseng C.A. Meyer) (인삼 신품종의 광합성 특성)

  • Lee, Sung-Sik
    • Journal of Ginseng Research
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    • v.26 no.2
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    • pp.85-88
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    • 2002
  • This study was carried out to obtain information of the photosynthetic rate at various temperature and light intensity, stomata, chlorophyll, specific leaf weight, characteristics of aerial part and root in ginseng new cultivars developed by pure line selection. The light saturation point of leaves in new cultivars and Jakyungjong were 15,000 lux, and the optimum air temperature on the photosynthesis of new cultivars and Jakyungjong were 20$\^{C}$. The photosynthetic rates were increased in order of Jakyungjong, Gopoong, Chunpoong and Yunpoong. The dark respiration rate of leaves in ginseng cultivars were increased according to the increasing of temperature, and the dark respiration rate of leaves of Yunpoong was the highest among cultivars. The specific leaf weight (SLW) were increased in order of Jakyungjong, Yunpoong, Gopoong, Chunpoong, but total chlorophyll contents were not different among cultivars. Stomata frequency of Yunpoong was the highest being 69.2ea among cultivars, while the length of stomata was reverse. Yunpoong was superior in aerial part among ginseng cultivars : the number of stem was 1.8ea, the number of palmately leaves was 7.7ea, the number of leaflets was 41.0ea, leaf area was 12.3 dm$^2$ The root weight were increased in order of Jakyungjong, Gopoong, Chunpoong and Yunpoong. Chunpoong and Gopoong hove good root shape the length of tap root in Chunpoong and Gopoong were the longest being 6.5 cm and 6.8 cm respectively, but that in Yunpoong was the shortest being 4.4 cm.