• Proceedings of the Korean Society of Crop Science Conference
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• 1987.06a
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• pp.20-21
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• 1987

• Journal of Microbiology and Biotechnology
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• v.30 no.5
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• pp.739-748
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• 2020

In this study, a method of heat adaptation was implemented in an attempt to increase the upper thermal threshold of two Streptococcus thermophilus found in South Korea and identified the alterations in membrane fatty acid composition to adaptive response to heat. In order to develop heat tolerant lactic acid bacteria, heat treatment was continuously applied to bacteria by increasing temperature from 60℃ until the point that no surviving cell was detected. Our results indicated significant increase in heat tolerance of heat-adapted strains compared to the wild type (WT) strains. In particular, the survival ratio of basically low heat-tolerant strain increased even more. In addition, the strains with improved heat tolerance acquired cross protection, which improved their survival ratio in acid, bile salts and osmotic conditions. A relation between heat tolerance and membrane fatty acid composition was identified. As a result of heat adaptation, the ratio of unsaturated to saturated fatty acids (UFA/SFA) and C18:1 relative concentration were decreased. C6:0 in only heat-adapted strains and C22:0 in only the naturally high heat tolerant strain were detected. These results support the hypothesis, that the consequent increase of SFA ratio is a cellular response to environmental stresses such as high temperatures, and it is able to protect the cells from acid, bile salts and osmotic conditions via cross protection. This study demonstrated that the increase in heat tolerance can be utilized as a mean to improve bacterial tolerance against various environmental stresses.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.241-251
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• 2014

This study was carried out to develop a simple, rapid and reliable assessment model to predict cold tolerance in Pittosporum tobira, a broad-leaved evergreen commonly used in the southern region of South Korea, which can minimize the possible experimental errors appeared in a electrolyte leakage test for cold tolerance assessment. The modeling procedure comprised of regrowth test and a electrolyte leakage test on the plants exposed to low temperature treatments. The lethal temperatures estimated from the methodological combinations of a electrolyte leakage test including tissue sampling, temperature treatment for potential electrical conductivity, and statistical analysis were compared to the results of the regrowth test. The highest temperature showing the survival rate lower than 50% obtained from the regrowth test was $-10^{\circ}C$ and the lethal was $-10^{\circ}C{\sim}-5^{\circ}C$. Based on the results of the regrowth test, several methodological combinations of electrolyte leakage tests were evaluated and the electrolyte leakage lethal temperatures estimated using leaf sample tissue and freeze-killing method were closest to the regrowth lethal temperature. Evaluating statistical analysis models, linear interpolation had a higher tendency to overestimate the cold tolerance than non-linear regression. Consequently, the optimal model for cold tolerance assessment of P. tobira is composed of evaluating electrolyte leakage from leaf sample tissue applying freeze-killing method for potential electrical conductivity and predicting lethal temperature through non-linear regression analysis.

• Weed & Turfgrass Science
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• v.2 no.3
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• pp.292-297
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• 2013

Winter turfgrass injury is one of the critical problems of many golf courses in Korea. Turfgrass loss from freezing injury due to low temperature leads to many types of damages including weed invasion, increased herbicide cost, increased soil erosion, and expensive re-establishment. Although Kentucky bluegrass (Poa pratensis L.) which is the most widely used among cool-season grasses in Korea is well known as cold tolerance species, freezing injuries to Kentucky bluegrass during winter are often found. Protecting the turfgrass crown is necessary to recover from low temperature stress in winter because shoots and roots can be recovered from the crown. Turf covers may protect the crowns from direct low temperatures and desiccation. Six different turf covers were installed to cover Kentucky bluegrass during a period of low temperatures. Turf covers had positive effects for spring green-up of Kentucky bluegrass based on the study. Applying any type of turf covers on Kentucky bluegrass resulted in an increase average and minimum temperature compared to the uncovered plot. Among turf covers, clear PVC film without holes produced the longest root length and the highest turfgrass quality.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2002.05a
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• pp.192-196
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• 2002

This paper describes on the fabrication and characteristics of a metal thin-film pressure sensor based on Cr strain-gauges for harsh environment applications. The Cr thin-film strain-gauges are sputter-deposited onto a micromachined Si diaphragms with buried cavity for overpressure protectors. The proposed device takes advantages of the good mechanical properties of single-crystalline Si as diaphragms fabricated by SDB and electrochemical etch-stop technology, and in order to extend the operating temperature range, it incorporates relatively the high resistance, stability and gauge factor of Cr thin-films. The fabricated pressure sensor presents a low temperature coefficient of resistance, high-sensitivity, low non-linearity and excellent temperature stability. The sensitivity is 1.097~1.21 $mV/V{\cdot}kgf/cm^2$ in the temperature range of $25{\sim}200^{\circ}C$ and the maximum non-linearity is 0.43 %FS.

• Journal of the Korean Institute of Landscape Architecture
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• v.39 no.6
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• pp.118-125
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• 2011

This study was conducted to check danger of the cold injury by reviewing the lowest temperature by regions and the cold tolerance of Lagerstroemia indica and Pyracantha angustifolia. The cold tolerance of the samples treated at low temperature was evaluated by measuring electrical conductivity and observing browning of the cambium. It was proved that the lethal temperature of L. indica is below $-17.1^{\circ}C$, and that of P. angustifolia is below $-18.9^{\circ}C$. The frequency of the lowest temperature went down below $-17.1^{\circ}C$, was 7 times in Daejeon, 55 times in Cheongju and 72 times in Suwon for the last 45 years. In Daejeon, it has happened only once since 1975. The temperature dropped below $-18.9^{\circ}C$ 45 times in Cheongju and 32 times in Suwon during the same period, but it has not happened at all since 1991. It appears that the rising coefficient of the lowest temperature increases, as the latitude gets higher. As a result, L. indica and P. angustifolia which were planted in the central region of Korea, are considered in danger from freezing injury because the temperature can get very low intermittently in winter. But, the risk of freezing has reduced gradually as the lowest temperature raises each year.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.3
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• pp.198-205
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• 1982

In 1980, rice was considerably damaged by abnormal low temperature. In this paper, to determine the effect of low temperature on the growth and nutrition of rice, the experiment were carried out: varietal response to low temperature in the region with different elevations. Regional differences of heading response to low temperature were observed among varieties. The difference of days between regions were bigger in tongil lines than Japonica lines. Especially, Milyang 42 and Hangangchalbeo might belong the cold suceptible group, since the varieties were severely delayed their heading in the high mountainous region as comparred to plain region. The delay of heading with low temperature was brought out grain sterility, and fertility and ripening ratio is influenced cold tolerance and elevations, and it's decreased yield. Varieties with higher grain sterility by low temperature have higher total nitrogen content, but tended to have lower potassium and phosphate contents in the flag leaf. High content of total nitrogen, low contents of potassium and silicate were observed in the sterilized grain chaff an the opposite result were noted in the ferilized grain chaff at the ripening stage. The results reveal that the balance of these mineral element may play an important role in ripening and possibly cold tolerance.

• Journal of Plant Biotechnology
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• v.49 no.2
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• pp.118-123
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• 2022

To obtain information on the molecular mechanism underlying the low temperature tolerance of sweet potato [Ipomoea batatas (L.) Lam], the proteome expressed in the sweet potato cultivar Xushu 15-1 with high cold storage tolerance and in the cultivar Xushu 15-4 with low cold storage tolerance was analyzed using 2-D and MALDI-TOF/TOF analyses. Compared with the control (without cold treatment), four protein spots were newly expressed in Xushu 15-1. The expression level of one protein spot was higher in Xushu 15-4 than in Xushu 15-1. Spot 2, which was newly expressed in Xushu 15-1, was identified as sporamin. Assessment of the change in protein expression levels over 8 weeks in the storage roots of the two cultivars treated at 4℃ revealed no significant difference in the expression levels in Xushu 15-1 over time. However, in Xushu 15-4, the expression level of one protein spot increased, while those of four spots decreased. Of the proteins with reduced expression levels, spots 7 and 8 were identified as actin and spots 9 and 10 were identified as fructokinase-like proteins. The present results are expected to enhance the understanding of the complex mechanism underlying the low temperature tolerance of sweet potatoes during storage and can be used to identify candidate genes for the development of new varieties of sweet potatoes with improved low temperature tolerance during cold storage in the future.

• Korean Journal of Plant Tissue Culture
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• v.27 no.4
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• pp.339-343
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• 2000

When plants are exposed to subfreezing temperatures ice crystals are forming within extracelluar space in leaves. The growth of ice crystal is closely related to the degree of freezing injury. It was shown that an antifreeze protein binds to an ice nucleator through hydrogen bonds to prevent growth of ice crystal and also reduces freezing damage. The antifreeze proteins in plants are similar to PR proteins but only the PR proteins induced upon cold acclimation were shown to have dual functions in antifreezing as well as antifungal activities. Three of the genes encoded for CLP, GLP, and TLP were isolated from barley and Kentucky bluegrass based on amino acid sequence revealed after purification and low temperature-inducibility as shown in analysis of the protein. The deduced amino acid of the genes cloned showed a signal for secretion into extracellular space where the antifreezing activity sup-posed to work. The western analysis using the antisera raised against the antifreeze proteins showed a positive correlation between the amount of the protein and the level of freeze tolerance among different cultivars of barely. Besides it was revealed that TLP is responsible for a freeze tolerance induced by a treatment of trinexapac ethyl in Kentucky bluegrass. Analysis of an overwintering wild rice, Oryza rufipogon also showed that an acquisition of freeze tolerance relied on accumulation of the protein similar to CLP. The more direct evidence for the role of CLP in freeze tolerance was made with the analysis of the transgenic tobacco showing extracellular accumulation of CLP and enhanced freeze tolerance measured by amount of ion leakage and rate of photosynthetic electron transport upon freezing. These antifreeze proteins genes will be good candidates for transformation into crops such as lettuce and strawberry to develop into the new crops capable of freeze-storage and such as rose and grape to enhance a freeze tolerance for a safe survival during winter.

• Journal of Plant Biology
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• v.17 no.4
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• pp.31-37
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• 1974

Seedlings of Nongbaek representing strong cold tolerant variety of rice plant, Jinheung of medium variety and Tongil of weak variety were used respectively for the present study. These seedlings were water cultured in phytotron which maintained at the daytime and night temperature of 30-$25^{\circ}C$, 20-2$0^{\circ}C$, 20-15$^{\circ}C$ and 15-1$0^{\circ}C$. The growth rate of plant height, tillering rate, increase in dry weight and absorption pattern of important mineral nutrients at their early growth stage under each temperature conditions were observed. Generally, it appeared that Nongbaek was more active in the growth of plant height than Jinheung, and Jinheung was more active than Tongil under low temperature condition. The tillering rate of these three varieties was equally rapid while it was decreased in the order of weak cold tolerant variety, such as Tongil, Jinheung and Nongbaek as the temperature declined gradually. The dry weigh tincreasing curve showed almost the same pattern in the varieties at the treatment of each temperature. Under low temperature conditions, Nongbaek showed higher absorption rate of N per dry weight and higher absorption amount of per plant body, Jinheung followed and Tongil was the lowest. The absorption amount of P2O5 increased in the top part compared with the roots as temperature rose and decreased as temperature declined. There seemed to be no difference of absorption among the varieties which have different cold tolerance each other. Under low temperature the absorption rate of K per dry weight was high, as a whole, especially Nongbaek was markedly higher than the other two. The absorption rate of Ca, Mg and Fe was also equally high in all varieties under low temperature and Nongbaek showed a more absorptive tendency in the absorption amount under low temperature.