• Title/Summary/Keyword: low-sodium

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Improved RNA extraction for fruit tree viruses in RT-PCR assay

  • Lee, Sin-Ho;Kim, Hyun-Ran;Kim, Jae-Hyun;Kim, Jeong-Soo
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.139.1-139
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    • 2003
  • Tissues from woody plant contain higher amount of phenolic compounds and polysaccharides, which give inhibitory effects on reverse transcriptase and/or Taq ploymerase. The common multiple-step protocols using several additives to inhibit polyphenoic compounds during nucleic acid extraction are time consuming and laborious. Sodium sulfite (Na$_2$SO$_3$) was used as inhibitor of polyphenolic oxidases in extraction buffer and compare it's effect between commercial RNA extraction kit and small-scale double-stranded RNA (dsRNA) extraction by RT-PCR. During nucleic acid extraction procedure, addition of 0.5%-1.5% (w/v) sodium sulfite to Iysis buffer or STE buffer resulted in lighter color change than extracts without sodium sulfite and improve the RT-PCR detection. When commercial RNA extraction kit used, optimal concentration of sodium sulfite were variable according to the host plant. However, using dsRNA as RT-PCR template, 1.5% sodium sulfite in STE buffer improves the detection of both viruses and unspecific amplifications were reduced significantly, Furthermore, when viruses existed at low titers in host plant, small-scale dsRNA extractions were very reliable.

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Localization Development of On-Site High Sodium Hypochlorite Generation (고농도 차아염소산나트륨 발생장치 국산화 개발)

  • Kim, Jung Sik;Shin, Hyun Su;Lee, Eun Kyoung;Jung, Bong Ik
    • Transactions of the KSME C: Technology and Education
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    • v.1 no.1
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    • pp.83-90
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    • 2013
  • The purpose of this study is to replace existing liquid chlorine with localization of on-site high (12%) sodium hypochlorite generation system. On-site high (12%) sodium hypochlorite generation system is higher the current efficiency of 38.7%, 54.6% reduction of salt consumption, and 97.3% lower rate of chlorate than on-site low (0.8%) sodium hypochlorite generation system.

Concentrations of Total Protein, Bilirubin, Sodium and Potassium in Blood Sera and Thymol Turbidity of Race Horses (경주마(競走馬) 혈청(血淸)의 단백질(蛋白質), Bilirubin, Sodium 및 Potassium 농도(濃度)와 Thymol Turbidity)

  • Shin, Kwang Soon;Rha, Dong Jin;Moon, Hi Joo;Cho, Jong Hoo
    • Korean Journal of Veterinary Research
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    • v.14 no.1
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    • pp.23-27
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    • 1974
  • Concentration of total protein, bilirubin, sodium and potassium in blood sera, and thymol turbidity of race horses, 78 males and 57 females aging from 2 to 5 years, were estimated. The results obtained were as follows: 1. Serum of race horses was low in protein concentrations as mean values of $5.48{\pm}0.77$ g/100ml. 2. Serum bilirubin was in normal range as mean values of total bilirubin, direct bilirubin and indirect bilirubin were $1.05{\pm}0.57$, $0.12{\pm}0.05$ and $0.93{\pm}0.58$ mg/100ml, respectively. 3. Mean value of serum thymol turbidity was $1.41{\pm}0.72$ S-H unit. 4. Mean values of serum sodium and potassium of race horses from Australia were $143{\pm}8.7$ and $3.85{\pm}0.53$ mEq/liter, respectively, and mean values of serum sodium and patassium of race horses from Japan were $142{\pm}9.4$ and $3.58{\pm}0.60$ mEq/liter, respectively.

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Processing of Kaolin-Based Microfiltration Membranes

  • Eom, Jung-Hye;Kim, Young-Wook;Song, In-Hyuck
    • Journal of the Korean Ceramic Society
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    • v.50 no.5
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    • pp.341-347
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    • 2013
  • Kaolin-based membranes with a pore size of 0.30-0.40 ${\mu}m$ were successfully prepared by a simple pressing route using low-cost starting materials, kaolin and sodium borate. The prepared green bodies were sintered at different temperatures ranging between 900 and $1200^{\circ}C$. The sintered membranes were characterized by X-ray diffraction, mercury porosimetry, scanning electron microscopy, and capillary flowmetry. It was observed that the porosity decreased with an increase in both the sintering temperature and the sodium borate content, whereas the flexural strength increased with an increase in both the sintering temperature and the sodium borate content. The air flow rate decreased with an increase in the sodium borate content. The typical porosity, flexural strength, and specific flow rate of the kaolin-based membrane sintered with 5 wt% sodium borate at $1100^{\circ}C$ were 37%, 19 MPa, and $1{\times}10^{-3}L/min/cm^2$, respectively, at a p of 30 psi.

Assessment of compressibility behavior of organic soil improved by chemical grouting: An experimental and microstructural study

  • Ghareh, Soheil;Kazemian, Sina;Shahin, Mohamed
    • Geomechanics and Engineering
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    • v.21 no.4
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    • pp.337-348
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    • 2020
  • Tropical organic soils having more than 65% of organic matters are named "peat". This soil type is extremely soft, unconsolidated, and possesses low shear strength and stiffness. Different conventional and industrial binders (e.g., lime or Portland cement) are used widely for stabilisation of organic soils. However, due to many factors affecting the behaviour of these soils (e.g., high moisture content, fewer mineral particles, and acidic media), the efficiency of the conventional binders is low and/or cost-intensive. This research investigates the impact of different constituents of cement-sodium silicate grout system on the compressibility behaviour of organic soil, including settlement and void ratio. A microstructure analysis is also carried out on treated organic soil using Scanning Electron Micrographs (SEM), Energy Dispersive X-ray spectrometer (EDX), and X-ray Diffraction (XRD). The results indicate that the settlement and void ratio of treated organic soils decrease gradually with the increase of cement and kaolinite contents, as well as sodium silicate until an optimum value of 2.5% of the wet soil weight. The microstructure analysis also demonstrates that with the increase of cement, kaolinite and sodium silicate, the void ratio and porosity of treated soil particles decrease, leading to an increase in the soil density by the hydration, pozzolanic, and polymerisation processes. This research contributes an extra useful knowledge to the stabilisation of organic soils and upgrading such problematic soils closer to the non-problematic soils for geotechnical applications such as deep mixing.

Effect of reducing sodium chloride based on the sensory properties of meat products and the improvement strategies employed: a review

  • Kim, Tae-Kyung;Yong, Hae In;Jung, Samooel;Kim, Hyun-Wook;Choi, Yun-Sang
    • Journal of Animal Science and Technology
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    • v.63 no.4
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    • pp.725-739
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    • 2021
  • Many consumers are concerned about the high levels of salt intake owing to the accompanied risk of chronic diseases. Due to this dietary concern, the food industry has recommended the reduction of salt content in many products. However, the addition of salt to meat products improves their quality and sensory properties, including saltiness, color, juiciness, and texture. Because quality deteriorations could induce decreased sensory scores owing to salt reductions, the challenges involved in improving the quality of reduced-salt meat products have been addressed. During the development of low-salt meat products, it is important to reduce sodium content and address the problems that arise with this reduction. Modified salt, organic acids, amino acids, nucleotides, hydrocolloids, high-pressure, ultrasound, electric pulsed field, and irradiation have been suggested as strategies to replace or reduce sodium content, and sensory scores could be improved by these strategies. Therefore, when developing a low-salt meat product, several perspectives must be considered and the latest technologies that could resolve this problem should be adopted.

Association between dietary sodium intake and disease burden and mortality in Koreans between 1998 and 2016: The Korea National Health and Nutrition Examination Survey

  • Park, Clara Yongjoo;Jo, Garam;Lee, Juhee;Singh, Gitanjali M.;Lee, Jong-Tae;Shin, Min-Jeong
    • Nutrition Research and Practice
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    • v.14 no.5
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    • pp.501-518
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    • 2020
  • BACKGROUND/OBJECTIVES: Sodium intake is positively associated with blood pressure, which may increase the risk for cardiovascular disease (CVD). Therefore, we assessed the disease burden of CVD attributable to sodium intakes above 2,000 mg/day and prospectively investigated the association between dietary/urinary sodium levels and the risk of all-cause and CVD-mortality using the Korea National Health and Nutrition Examination Survey (KNHNES). SUBJECTS/METHODS: A total of 68,578 and 33,113 participants were included for comparative risk assessment (CRA) analysis and mortality analysis, respectively, and mean follow-up time for mortality was 5.4 years. CRA analysis was used to quantify attributable incidences of stroke, ischemic heart disease (IHD), and deaths attributable to sodium intake between 1998 and 2016. Cox proportional hazard regression model was used to determine the association between sodium intake and all-cause and CVD-mortality. RESULTS: Mean dietary sodium intake decreased over time, reaching 3,647 mg/day in 2016. Similarly, the population attributable fractions of stroke and IHD, and the number of CVD-associated deaths attributable to high sodium intake/excretion also decreased. In terms of association with mortality, when participants were grouped into quartiles (Q) by energy-adjusted sodium intake, those in Q2 had a lower risk of all-cause mortality than those in Q1 with lower intakes. The risk of CVD-associated mortality was higher only in females with high sodium intake in Q4 than those in Q1. CONCLUSIONS: This nationwide data indicates that, in line with previous studies of multiple cohorts, both low and high sodium intakes may be associated with an increased risk of mortality; therefore, the optimal sodium intake for Koreans needs to be revised.

Microencapsulation of Fish Oil by Low-molecular Weight Sodium Alginate (저분자 알긴산을 이용한 fish oil의 microencapsulation)

  • Cho, Min;Kim, Byung-Yong;Baik, Moo-Yeol;Rhim, Jong-Hwan
    • Korean Journal of Food Science and Technology
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    • v.37 no.5
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    • pp.730-735
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    • 2005
  • Possibility of using low-molecular weight alginate as a wall material for encapsulation of fish oil was investigated. Encapsulation yield increased with increasing calcium chloride concentration up to 5% and was maintained thereafter, whereas slightly increased with increasing sodium alginate concentration up to 1.25% and decreased dramatically thereafter; emulsifier concentration had no effect on encapsulation yield. Loading efficiency increased with increasing content of core material. Encapsulation yields of low- and high-molecular weight alginates were similar, indicating low-molecular weight alginate can be used as wall material for encapsulation of fish oil.

Effect of sodium stearoyl-2-lactylate (PROSOL®) supplementation on growth performance and nutrient digestibility in growing pigs

  • Vetriselvi Sampath;In Ho Kim
    • Korean Journal of Agricultural Science
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    • v.49 no.1
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    • pp.45-50
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    • 2022
  • The aim of this study is to evaluate the effects of supplementation of low-energy diets with PROSOL® (sodium stearoyl-2-lactylate) as an emulsifier on the growth performance and nutrient digestibility of growing pigs. A total of 120 crossbred ([Landrace × Yorkshire] × Duroc) growing pigs with an average initial body weight of 23.80 ± 4.87 kg were used in a 56-day feeding trial and were sorted into three dietary treatments. Each treatment utilized eight replications with five pigs per pen. The dietary treatments were as follows: PC (high, net energy diet [NE] = 2,488 kcal·kg-1 [weeks 1 to 4] and NE = 2,477 kcal·kg-1 [weeks 5 to 8]); NC (low, NE = 2,472 kcal·kg-1 [weeks 1 to 4] and NE = 2,468 kcal·kg-1 [weeks 5 to 8]); NC1 (NC + 0.05% PROSOL® emulsifier). Growth performance outcomes were measured at the initial point and at weeks 4 and 8 while nutrient digestibility parameters were measured at weeks 4 and 8. From weeks 1 - 8, body weight and the average daily gain of the NC1 group pigs showed significant increases (p < 0.05) compared to those of the PC and NC treatment groups. At the end of the trail, pigs fed a diet containing low net energy with 0.05% of the emulsifier showed reduced feed conversion ratio (FCR) levels. However, the average daily feed intake and nutrient digestibility of dry matter and nitrogen remained unaffected throughout the experiment. In summary, the addition of a low-net-energy diet with 0.05% sodium stearoyl-2-lactylate as an emulsifier enhanced the body weight and daily gain with no adverse effects on nutrient digestibility in growing pigs.

Antioxidant activity and metabolic regulation of sodium salicylate on goat sperm at low temperature

  • Wenzheng Shen;Yu Fu;Haiyu Bai;Zhiyu Zhang;Zhikun Cao;Zibo Liu;Chao Yang;Shixin Sun;Lei Wang;Chunhuan Ren;Yinghui Ling;Zijun Zhang;Hongguo Cao
    • Animal Bioscience
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    • v.37 no.4
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    • pp.640-654
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    • 2024
  • Objective: The purpose of this study was to explore the effect of sodium salicylate (SS) on semen preservation and metabolic regulation in goats. Methods: Under the condition of low temperature, SS was added to goat semen diluent to detect goat sperm motility, plasma membrane, acrosome, antioxidant capacity, mitochondrial membrane potential (MMP) and metabonomics. Results: The results show that at the 8th day of low-temperature storage, the sperm motility of the 20 μM SS group was 66.64%, and the integrity rates of the plasma membrane and acrosome were both above 60%, significantly higher than those of the other groups. The activities of catalase and superoxide dismutase in the sperm of the 20 μM SS group were significantly higher than those of the control group, the contents of reactive oxygen species and malondialdehyde were significantly lower than those in the control group, the MMP was significantly higher than that in the control group, and the contents of Ca2+ and total cholesterol were significantly higher than those in the control group. Through metabonomics analysis, there were significant metabolic differences between the control group and the 20 μM SS group. Twenty of the most significant metabolic markers were screened, mainly involving five metabolic pathways, of which nicotinic acid and nicotinamide metabolic pathways were the most significant. Conclusion: The results indicate that SS can effectively improve the low-temperature preservation quality of goat sperm.