• 한국작물학회지
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• 제53권2호
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• pp.187-195
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• 2008

Polyphenol oxidase (PPO) is implicated in discoloration of white salted noodles and other wheat based foods. PPO activity was evaluated to determine the effect on discoloration of noodle dough sheets prepared from 25 Korean wheat flours during storage and to screen experimental lines with low PPO activity in 52 Korean wheats. PPO activity was assayed with whole-seed and performed with L-dihydroxyphenylalanine (L-DOPA) as substrates. Absorbance by L-DOPA assay of 25 Korean wheats was from 0.285 to 1.368 at 475 nm. PPO activity was significantly related with grain characteristics, including 1000-kernel weight and grain colors. In flour characteristics, PPO activity positively correlated with ash and protein content (r = 0.658, P < 0.001 and r = 0.424, P < 0.05, respectively) and negatively correlated with $L^*$ value of flour (r = 0.412, P < 0.05). In the changes of color of noodle dough sheet, $L^*$ and $b^*$ values consistently decreased and $a^*$ value increased during storage. PPO activity negatively correlated with $L^*$ value of noodle dough sheet during storage (r = 0.566, P < 0.01 at 2 hr, r = 0.547, P < 0.01 at 24 hr, and r = 0.509, P < 0.01 at 48 hr). But, no significant relationship was found in between PPO activity, $a^*$ and $b^*$ values during storage. The 52 Korean wheat lines examined in this study were divided into 3 different groups, low (< 0.500), medium (0.501-0.999) and high level (> 1.000), on the basis of the level of PPO activity. Twenty two Korean wheat lines showed low level of PPO activity and Suwon 252, 277 and 280 showed lower PPO activity (< 0.200) than others.

• 한국수산과학회지
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• 제28권6호
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• pp.812-813
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• 1995

Lactococcal cells are nutritionally fastidious and thus, generally cultured either in milk or M17 medium (Terzaghi and Sandine, 1975). In this study, Lactococcus cremoris wild-type (KH) and its less­proteolytic mutant (KHA1) cells were grown on the M17 medium or with modified M17 medium by replicated parallel experiments. The modified M17 medium had the same composition as M17 medium, except that lactose was replaced by glucose. Analyses of culture-broth samples, in which the M17 and the modified M17 media were used, were conducted by high-performance liquid chromatography (HPLC). But, working with these media created noisy problems in analyses of samples. Therefore, a new semi-synthetic medium was developed on the basis of nutritional requirements (Morishita et al., 1981). The composition of the semi-synthetic medium determined on the basis of the nutritional requirements and the composition of milk, is presented in Table 1. The composition of M17 medium is also presented and compared in the table. L. cremoris KH and KHA1 cells were grown again on the new synthetic medium containing glucose or lactose. The broth samples were then drawn and analyzed by HPLC. Clearer separations of fermented products were achieved from the new medium than those with the M17 and the modified M17 media. In comparison with the M17 or the modified M17 media, growth on the new medium was good (Kim et al, 1993). Additional fermentations were also carried out at a controlled pH of 7.0, where enhanced growth of lactococcal cells was obtained. In the fermentations, samples were also analyzed for the concentrations of sugar and lactic acid. The results showed that the new synthetic medium was as good as or better than the M 17 and the modified M 17 media. This is because casein hydrolysate in the synthetic medium provided a ready supply of amino acids and peptides for L. cremoris KH and KHA1 cells. Lactic acid bacteria (LAB) including Lactococcal cells have been known to be an effective means of preserving foods, at the same time as giving particular tastes in fields of dairy products. LAB also have always occupied an important place in the technology of sea products, and marine LAB have known to be present in traditional fermented products (Ohhira et al, 1988). To apply the new synthetic medium to marine LAB, two different LAB were isolated from pickled anchovy and pollacks caviar and were grown on the new media in which various concentrations of NaCl $(3, 5, 7 and 10\%)$ added. They were also grown on the medium solution in natural seawater $(35\%o\;salinity)$ and on the solution of natural seawater itself, too. As seen in Fig. 1, Marine LAB were grown best on the synthetic medium solution in natural seawater and the higher concentrations of NaCl were added to the medium, the longer lag-phase of growth profile appeared. Marine LAB in natural seawater were not grown well. From these results, the synthetic medium seems good to cultivate cells which are essential to get salted fish aged. In this study, it showed that the new synthetic medium provided adequate nutrition for L. cremoris KH and KHA1 cells, which have been used as cheese starters (Stadhouders et al, 1988). Using this new medium, the acid production capability of starter cultures could be also measured quantitatively. Thus, this new medium was inferior to the M17 or the modified M17 medium in culturing the cheese starters and in measuring fermentation characteristics of the starter cells. Moreover, this new medium found to be good for selected and well-identified marine LAB which are used in rapid fermentations of low-salted fish.

• 동아시아식생활학회지
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• 제3권2호
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• pp.99-106
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• 1993

The aim of this study is to elevate the efficiency of salting with low salt concentration in order to improve the quality of salted Chinese cabbage. The efficiency of salting was tested with various sub-atmosphere(760mmHg to 0mmHg), temperature(20$^{\circ}C$ and 30$^{\circ}C$), and concentration of salt(3%, 5% and 10%). The salting efficiency was estimated by amounts of juice, calcium elution, sodium penetration and the state of tissue. The amunts of juice eleuted from Chinese cabbage was increased significantly with the decrease of atmosphere and the time to reach the highest amounts was reduced. This tendency was promoted, with increase of salinigy(from 3% to 10%)and the increase of temperature(more juice at 30$^{\circ}C$ than 20$^{\circ}C$). The amounts of eluted calcium from Chinese cabbage tissue during salting under sub-atmosphere was directly proportional to amounts of juice and was inversely proportional to penetrated sodium. The salting time was reduced with elevation of sub-atmosphere. It was desirable to take between 5.5 and 9 hours to salt under the condition of 3% of salting, 20$^{\circ}C$, and 560-0mmHg. It took between 4.4 and 5 hours under 5% of salt and between 4 and 4.5 hours under 10% of salt. However, it was undesirable because the drying phenomenon and the transparency of tissue appeared under 30$^{\circ}C$ and 160-0mmHg.

• 한국식품조리과학회지
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• 제7권4호
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• pp.111-118
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• 1991

The purpose of this study was to evaluate nutritional adequacy and microbiological quality of foods served in day-care centers, and to provide basic information and guidelines for the development of computerized standardized recipes and microbiological quality control program. Assessment was conducted for four day-care certers selected from each of the four groups categorized in the basic survey. Nutritional balance and amount of consumption of the food was calculated and microbiological analysis was conducted. The results of the study showed the followings: the nutritional assessment of food served in day-care centers indicated that energy and nutrients contents of foods were below the recommended level (RDA/3), except the case of a university day-care center; the percentage of leftovers for soups and vegetables was high, but that for a la carte was low; fecal coliform was not found in any of samples, however, the number of coliform in the salted Korean radish and salad was higher than guideline for microbial acceptability; the number of coliform checked from the surface of the cutting board was higher than acceptable level in most day-care centers.

• 한국미생물·생명공학회지
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• 제19권2호
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• pp.200-207
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• 1991

Jariieot is a local food prepared by fermentation of salted fish, Chromis notatus. Since its NaC' content is around 20% like other fermented seafoods, reduction NaCl concentration is desirable to minimize the risk of health hazard. Addition of KCl and enrichment of lactic acid fermentation were attempted to solve the problems resulting from low salt concentration. NaCl and KCl were added to a fish, Chromis notatus simultaneously at concentrations of 10 to 4% and 5 to 2%, respectively. Lactic acid bacteria and glucose at final concentration of 2% were also mixed with the above-salt treated fish to prepare jarijeot. The jarijeot was examined periodically for chemical changes during aging and compared with reference jarijeot containing only 20% of NaCl to find out an appropriate method for quality improvement. The content of ATP and its related compounds was not affected by the concentration of NaCl or the presence of lactic acid bacteria. Nearly no difference in contents of free amino nitrogen, trimethylamine oxide, trimethylamine and volatile basic nitrogen was observed between the jarijeot containing 20% of NaCl only and that containing 10% of NaCl, 5% of KCl, 2% of glucose and cells of Pediococcus halophilus. Moreover, sensory evaluation of both kinds of jarijeots revealed almost the same scores. The number of cells of P. halophilus was maintained at concentration of $10^5$cell/ml for 60days' fermentation in the above mentioned jarijeot containing 10% of NaCl. Its pH was dropped down to 4.2. Accordingly it is possible to prepare jarijeot enriched with lactic acid bacteria if KCl and glucose are added at concentration of 5% and 2%, respectively, in addition to NaCl at a final concentration of 10%.

• 한국식생활문화학회지
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• 제8권3호
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• pp.267-274
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• 1993

무의 염장과정 중 소금의 침투량, 담금액의 pH와 산도 및 조직감의 변화와 이와 관련된 화학적 변화, 효소활성 변화를 알아보고자 하였다. 그 결과는 다음과 같다. 1. 3%와 10% 소금용액에서 생무와 열처리한 무를 담구어 4일간 저장하였을 때, 무 조직 내의 소금농도는 2일까지는 빠르게 증가하였으며 침투속도는 10% 소금용액에서 더욱 컸으며 열처리구에서 더 높았다. 2. 무의 염장과정 중 담금액의 pH는 낮아지고 산도는 증가하여 염장 10일째에 pH는 3.45, 산도는 0.52%를 나타내었다. 3. 힘-거리곡선의 형태는 생무의 경우, 파열점(a)과 최대 압착변형점(c)이 일치하거나 a 이후에 더 큰 c가 나타나서 positive slope을 갖는 곡선이 주종을 이루나, 염장한 무는 a와 c가 일치하면서 특징적인 하나의 peak만을 나타내거나 혹은 a 이후에 최대변형력이 작아지면서 negative slope의 곡선이 주로 나타났다. 또한, 염장이 진행됨에 따라 무의 peak 수는 점차 줄어들었다. 압착시험, 침투관통시험 및 절단시험을 통하여 무의 조직감 변화를 측정한 결과, 무의 압착변형력은 염장과정 중 전반적으로 감소하였다. 크기가 다른 탐침을 이용하여 침투관통시험을 행한 결과, 전반적으로 침투관통력은 감소하였으며 탐침의 직경이 클수록 침투관통력은 증가하는 것으로 나타났다. 또한 절단력은 염장과정 중 증가하였다. 이로부터 염장과정 중 무의 견고성은 감소하였으며 무의 질긴정도(toughness)는 증가하는 것으로 생각된다. 4. AIS로부터 펙틴질을 분획하여 그 함량을 측정한 결과, 염장과정 중 HWSP 함량과 HXSP 함량은 증가하였으며 HClSP 함량은 감소하였다. 5. 염장과정 중 효소 활성의 변화는 발효 4일째까지 증가하다가 감소하였으며 무 염장액에서보다 무 고형분에서의 PG 활성이 크게 나타났다. PE 활성은 염장과정 중 무 고형분과 무 염장액에서 계속 감소하였다. 한편, Cx-cellulase은 염장 초기에는 거의 활성을 나타내지 않았으나 염장 2일째부터 활성이 나타나기 시작하였다.

• 한국식품저장유통학회지
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• 제14권1호
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• pp.24-29
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• 2007

월동배추의 저장방법에 따른 특성과 김치를 위해 절였을 경우 특성을 조사하여 배추의 효과적인 저장방법을 구명하고자 하였다. 저장방법(PE 필름백, 플라스틱 컨테이너, 망저장, 움저장)에 따른 배추의 특성을 3개월 동안 조사한 결과, PE 필름백에 저장한 배추가 겉잎이 부패하는 단점이 있으나 다른 처리에 비해 감모율, 정선 손실율, 외관 등에서 비교적 좋은 결과를 보여주었다. 그러나 저장방법을 달리하여 배추를 절였을 경우에 처리별로 절임특성이 큰 영향을 보이지 않았다. 본 실험 결과로 월동배추의 저장 방법이 절임 특성에는 큰 영향이 없지만 저장방법에 따라서 중량 감모 및 정선손실 정도에 영향을 미치므로 배추는 저장중의 이러한 손실을 줄이고 신선도를 유지할 수 있는 방법에 대한 연구가 보다 필요할 것으로 생각된다.

• 대한지역사회영양학회지
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• 제21권2호
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• pp.200-217
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• 2016

Objectives: The purpose of this study was to assess energy and nutritional intake and investigate the preference for food and cooking methods of the residents in elderly care facilities. Methods: Data were collected from 72 residents (10 males and 62 females) aged ${\geq}70$ years in elderly care facilities using questionnaires, food photographs for estimating dietary intake and records for daily physical activity. Results: Average age of the study participants was 85.0 years and 41, 36 and 8 had dementia, hypertension and diabetes mellitus, respectively. 15%, 65% and 19% of subjects were physically mobile, enervated, and immobile, respectively. Daily energy intake was 1360.2 kcal in men and 1378.0 kcal in women, which were 68.0% and 86.1% of the estimated energy requirement (EER) of dietary reference intake for Koreans (KDRI) for ${\geq}75$ year old individuals, respectively. Estimated energy expenditure (EEE) of subjects calculated using formula from KDRI was 1361.9 kcal and EER calculated using estimated daily physical activity (EDPA) was 1232.9 kcal. Energy intake and EEE from KDRI were higher than EER from EDPA. Dietary intake of dietary fiber, calcium, potassium, zinc, vitamin $B_2$, niacin, vitamin C were lower, and protein, phosphorous, iron, sodium, vitamin A, vitamin $B_1$, vitamin $B_6$, vitamin E were higher than the corresponding ones of KDRI. Subjects liked meats, fishes and shellfish, and fruits, while subjects disliked milk, seaweeds and salted fish and salted vegetables. Cooked rice, soybean paste soup, beef, cooked sliced radish strip, and yogurt were favorite foods, with steam being a favorite cooking method. Subjects considered nutrition as the most important factor for improving food service quality. Conclusions: Results of this study could be utilized for improving food-service for the residents in elderly care facilities, and provide a basis for setting reference intake of energy and nutrients of the elderly having very low activity levels.

• 한국식품영양과학회지
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• 제29권6호
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• pp.995-1002
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• 2000

본 연구는 in vitro에서 여러 식이섬유의 Na흡착효과를 측정하였고 다시마내의 alginate의 Na 흡착능을 측정하고 다시마 첨가김치의 소금(Na) 흡착기능으로 저염 기능성 김치를 개발하려고 하였다. 여러 식이섬유의 표품으로 Na흡착효과를 시험한 결과, alginate와 sodium alginate, 그리고 다시마에서 추출한 alginate가 가장 큰 효과가 있었다. Sodium alginate는 산에서도 용해된 상태로써 위장에서부터 Na를 흡착하여 (29.9%) 소장까지 내려가지만(33.8%) alginate와 다시마에서 추출한 alginate는 소장의 알칼리 상태에서 32.3%와 27.4%의 Na 흡착능을 보였다. 다시마내의 alginate함량은 건다시마의 경우 건조물당 22.2%, 염장다시마는 21.9%, 30분 침지 후3회 세척한 염장다시마는 19.8%였다 관능검사를 통하여 염장 다시마를 세척한 다시마를 0.5$\times$3 cm 형태로 절단하여 김치레시피의 30%첨 가한 김치를 제조하였다. 다시마김치의 이화학적 특성, 젖산균의 변화 그리고 관능검사를 통해 그 특성을 살펴 본 결과 대조김치에 비해 환원당 함량과 젖산균이 많았으며 발효속도는 다소 빨랐고 특히 다시마첨가 김치는 대조김치와 다시마젓갈첨가 김치보다 탄산미가 많고 군덕내가 적은 것이 특징이었다. 다시마첨가김치는 대조김치에 비해 수용성 식이섬유의 양이 건조물당 6.2%에서 9.2%로 증가되었다.

• 한국식생활문화학회지
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• 제25권3호
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• pp.241-250
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• 2010

This study was conducted to improve the kimchi intake for high school students of various kimchi sub-ingredients and foods using kimchi. The students believed that kimchi is good for health. The overall satisfaction, baechu quality and the taste and degree of fermentation of kimchi was high, but there was a low preference for offered kimchi kinds, kimchi subingredient, foods using kimchi. The types of kimchi preferred and often offered in school meals were baechu-kimchi and kkakdugi. The common kimchi sub-ingredients were radish and welsh onion in vegetables, squid and oyster in sea foods, saeu-jeot and myeolchi-jeot in salted fish, and pear and apple in fruits. The preference for kimchi sub-ingredients were high for sesame leaf and yeolmu in vegetables, saeu-sal and squid in sea foods, saeu-jeot and nakji-jeot in salted fish, and pear and apple in fruits. The foods using kimchi preferred and often offered with school meals were kimchi-jjigae, bokkeumkimchi, kimchi soup, kimchi-bokkeum-bap, and kimchi-jeon. The kimchi sub-ingredient for which students had the greatest preference was meats. Among the foods using kimchi with meats, the most preferred were kimchi-pyeonyuk bossam, doejigogi kimchi duruchigi, and kimchi galbi-jjim. Among the foods using kimchi with noodles, the most preferred were kimchi- bibimmyeon, kimchi -naengmyeon, and kimchi-cheese spaghetti. Among the foods using kimchi with vegetables, the most preferred were kimchi-pa-jeon, kimchi- deopbap and kimchi- goguma gui. Of the foods using kimchi with processed foods, the most preferred were kimchi-mandu, kimchi-bacon jumeok-bap and kimchi- cheese omelet. Among the foods using kimchi containing sea food, kimchi-haemul bokkeum-bap, kimchi-hoe-deopbap, and kimchi-saeu-jjim were most preferred. Overall, these results suggest that various kimchi sub-ingredients and foods using kimchi should be improved for kimchi intake of school meals.