• ALGAE
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• 제28권3호
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• pp.241-266
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• 2013

The mangrove algal flora of Guam and the Federated States of Micronesia has been poorly explored. We add to our knowledge of this region by observations of collections from these regions. This paper presents new and additional records of: Rhodophyta-Acrochaetium globosum, Colaconema sp., Caulacanthus indicus, Bostrychia moritziana / B. radicans, B. radicosa, B. simpliciuscula, B. kelanensis and B. tenella, Murrayella periclados, and Caloglossa ogasawaraensis; Chlorophyta-Boodleopsis carolinensis; and Phaeophyceae-Dictyota adnata, Dictyotopsis propagulifera, and Canistrocarpus cervicornis. Most specimens were cultured to investigate their reproductive biology and many specimens were further identified using molecular data. Low molecular weight carbohydrates (dulcitol, sorbitol, and digeneaside) were identified in samples of B. radicosa and B. simpliciuscula. We also present data on manganese-rich deposits found on B. simpliciuscula and B. tenella in culture, possibly formed by epiphytic bacteria.

• Journal of the Korean Wood Science and Technology
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• 제27권4호
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• pp.1-14
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• 1999

As the biodegradation of wood constituents has been understood as a multi-basidiomycetes and enzymatic processes, this review will focus on the roles of low molecular compounds and radicals working in harmony with fungal enzymes. Wood rotting basidiomycete fungi penetrate wood, and lead to more easily metabolize carbohydrates of the wood complex. The white-rot fungi, having versatile enzymes, are able to attack directly the "lignin barrier". They also use a multi-enzyme system including so-called "feedback" type enzymes allowing for simultaneous degradation of lignin and carbohydrates. The multi-enzymes including laccase support the proposed route by explaining how the high molecular weight enzymes can function in the wood complex. These enzymes may function separately or cooperate each other. In addition, veratryl alcohol oxidase, cellobiose dehydrogenase, arylalcohol dehydrogenase, and particularly low molecular mediators and radicals have an important role in wood biodegradation. However, the possibility of other mechanism as well as other enzymes, as operating as feedback systems in the process of wood degradation, could not be excluded.

• ALGAE
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• 제21권4호
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• pp.407-416
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• 2006

Low molecular weight carbohydrates, phycobilin pigments and cell structure using light and transmission electron microscopy were used to describe a new genus of unicellular red algae, Erythrolobus coxiae (Porphyridiales, Porphyrideophyceae, Rhodophyta). The nucleus of Erythrolobus is located at the cell periphery and the pyrenoid, enclosed by a cytoplasmic starch sheath, is in the cell center. The pyrenoid matrix contains branched tubular thylakoids and four or more chloroplast lobes extend from the pyrenoid along the cell periphery. A peripheral encircling thylakoid is absent. The Golgi apparatus faces outward at the cell periphery and is always associated with a mitochondrion. Porphyridium and Flintiella, the other members of the Porphyrideophyceae, also lack a peripheral encircling thylakoid and have an ER-mitochondria-Golgi association. The low molecular weight carbohydrates digeneaside and floridoside are present, unlike both Porphyridium and Flintiella, which have only floridoside. The phycobilin pigments B-phycoerythrin, R-phycocyanin and allophycocyanin are present, similar to Porphyridium purpureum. The cells have a slow gliding motility without changing shape and do not require substrate contact. The ultrastructural features are unique to members of the Porphyrideophyceae and recent molecular analyses clearly establish the validity of this new red algal class and the genus Erythrolobus.

• Asian-Australasian Journal of Animal Sciences
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• 제23권10호
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• pp.1386-1398
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• 2010

Soybean contains a high concentration of carbohydrates that consist mainly of non-starch polysaccharides (NSP) and oligosaccharides. The NSP can be divided into insoluble NSP (mainly cellulose) and soluble NSP (composed mainly of pectic polymers, which are partially soluble in water). Monogastric animals do not have the enzymes to hydrolyze these carbohydrates, and thus their digestion occurs by means of bacterial fermentation. The fermentation of soybean carbohydrates produces short chain fatty acids that can be used as an energy source by animals. The utilization efficiency of the carbohydrates is related to the chemical structure, the level of inclusion in the diet, species and age of the animal. In poultry, soluble NSP can increase digesta viscosity, reduce the digestibility of nutrients and depress growth performance. In growing pigs, these effects, in particular the effect on gut viscosity, are often not so obvious. However, in weaning piglets, it is reported that soy oligosaccharides and soluble NSP can cause detrimental effects on intestinal health. In monogastrics, consideration must be given to the anti-nutritive effect of the NSP on nutrient digestion and absorption on one hand, as well as the potential benefits or detriments of intestinal fermentation products to the host. This mirrors the needs for i) increasing efficiency of utilization of fibrous materials in monogastrics, and ii) the maintenance and improvement of animal health in antibiotic-free production systems, on the other hand. For example, ethanol/water extraction removes the low molecular weight carbohydrate fractions, such as the oligosaccharides and part of the soluble pectins, leaving behind the insoluble fraction of the NSP, which is devoid of anti-nutritive activities. The resultant product is a high quality soy protein concentrate. This paper presents the composition and chemical structures of carbohydrates present in soybeans and discusses their nutritive and anti-nutritive effects on digestion and absorption of nutrients in pigs and poultry.

• 대한본초학회지
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• 제34권6호
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• pp.131-138
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• 2019

Objective : Sparassis crispa has been recognized for its therapeutic value since the late 20th century because of its high β-glucan content. Since then, researches have been conducted on the pharmacological effects but most of these are individual studies on the effects of β-glucan from S. crispa and the comprehensive reviews are lacking. The purpose of this study was to review the compounds composition and pharmacological effects of S. crispa. Methods : This review analyzes the papers about chemical and nutritional composition and pharmacological effects of S. crispa. The data in this review is based on selected papers after reviewing all studies containing the keyword "sparassis crispa" for PubMed, NDSL, and J-Stage published before February 2019. Results : S. crispa is composed of protein, lipids, and carbohydrates. Most of the compounds are carbohydrates and the highest content is β-glucan. More than 40% of the dried fruiting body of S. crispa is composed of β-glucan. In addition, it contains polyphenols, flavonoids, terpenoids and phthalide-based compounds. Broad spectrum of its pharmacological actions have been established which include immunomodulatory, anticancer, antiinflammatory, antioxidant, hypoglycemic, antiobesic and neuroprotective effects. Conclusion : The most studied fields have been shown to have immunomodulatory and anticancer effects by inhibiting the proliferation of cancer cells and angiogenesis and increasing hematopoitic responses. Unique structure and characteristic of high molecular weight β-glucan are considered to have high immunomodulatory effects of S. crispa. And low molecular fractions or phthalides of S. crispa also have antioxidant, immunomodulatory and anticancer effects.

• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.560-567
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• 2005

Three groups of exopolysaccharides (EPSs) (designated as Fr-I, Fr-II, and Fr-III) were isolated from the culture filtrates of Paecilomyces sinclairii by gel filtration chromatography on Sepharose CL-4B. Their molecular characteristics were examined by multi-angle laser light scattering (MALLS) connected online to a size exclusion chromatography (SEC) and refractive index (RI) detector system. The weight-average molar mass of Fr-I, Fr-II, and Fr-III of EPSs were determined to be $1.540{\times}10^6,\;6.302{\times}10^4$, and $9.389{\times}10^4\;g/mol$, respectively. All three EPSs showed a fairly low polydispersity indice, ranging from 1.008 to 1.059 (nearly mono disperse behavior), and showed different carbohydrates and amino acids compositions; all fractions of EPSs consisted of mainly cystine, valine, and arginine in the protein moiety, and mainly ribose, galactose, and glucose in the carbohydrate moiety. The determination of gyration radii of the EPSs in SEC/MALLS analysis revealed the molecular shape of the Fr-I to be a rod-like structure, whereas the Fr-II and Fr-III had a random-coil structure in an aqueous solution.

• 한국식품과학회지
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• 제28권4호
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• pp.720-729
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• 1996

추청벼, 조생통일벼, IR 36등 세 품종의 쌀에 셀룰라제(cellulase) 처리가 팽윤력, 용해도, 아밀로그래프, differential scanning calorimetry (DSC), 가용성 탄수화물 용출 및 밥의 경도 등에 미치는 효과에 대하여 시험하였다. 효소처리에 의하여 세 품종의 쌀에서 모두 팽윤력과 용해도가 증가하였으며 아밀로그램 점도는 모두 감소하였다. 시차 주사 열량계(DSC)를 이용한 밥의 노화 시험에서는 효소 처리의 뚜렷한 효과가 없었다. 효소처리한 쌀의 가용성 탄수화물의 겔 크로마토그래피에서 분자량 10,000미만의 세포벽 분해산물이 확인되었다. Rheometer로 측정한 밥의 경도는 효소처리한 모든 품종의 쌀에서 감소하였다.

• 한국미생물·생명공학회지
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• 제23권3호
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• pp.269-274
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• 1995

For the screening of a new functional exopolysaccharide, sugar composition and rheological properties of exopolysaccharide produced from Xanthomonas sp. EPS-1 were investigated. The average molecular weight of exopolysaccharide was determined to be approximately 2.l $\times$ 10$^{6}$ dalton. The new exopolysaccharide EPS-1 was composed of mannose, glucose, galactose and gluco- samine. IR analysis showed that the exopolysaccharide EPS-1 was assumed to be polymer with carbohydrates. NMR analysis showed that exopolysaccharide EPS-1 was presumed to be 4 units of sugar and trace of CH$_{3}$ group. Exopolysaccharide EPS-1 solution showed a characteristic of non-Newtonian fluid properties. At the concentration of 1.0%, the consistency index and the flow behavior index were shown at 10.8352 poise-sec and 0.4419, respectively. All dispersions were pseudoplastic fluids described accurately by Power-law model. Exopolysaccharide EPS-1 was highly viscous at low concentration, with good stability over a wide range of pH 5 to 13. The excellent compatibility of exopolysaccharide EPS-1 was represented with salts such as sodium chloride.

• Animal cells and systems
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• 제2권4호
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• pp.455-462
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• 1998

A survey was conducted on the inorganic and organic solute patterns of plants in connection with nitrate metabolism according to different light regimes (1.9, 16.0, 91.5 $Wm^{-2}$). Besides measuring in vivo NRA, we also quantitatively analyzed ater-soluble inorganic ions, organic acids, low molecular weight carbohydrates, amino aciss and total N (% DW). Among 4 Carex species, C. pilosa is known as shade-adapted species and the others as half (C. gracilis) to full (C. rostrata & C. distans) light-adapted species. Compared to species adapted to high light intensity, shade-adapted C. pilosa showed reduced productivity under the highest light intensity. In general, nitrate and amino acid levels decreased at higher light intensity, while sugar and organic acid concentrations increased. In C. pilosa osmolality tended to rise with increasing light intensity, while in the other species it tended to fall. Under low light intensity, the drop in soluble carbohydrate contents is osmotically compensated for by an enhanced nitrate concentration. It is concluded that competition between nitrate and $CO_2$reduction for reductants and ATP from photosynthesis may have important ecological consequences for the adaptation of plants to low or high light conditions. Additionally, the patterns of ionic changes due to increased light intensities were essentially the same in all selected species, indicating similar characteristics of heir mineral ion and organic acid metabolism as well as in field-grown Carex species.

• Korean Chemical Engineering Research
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• 제50권2호
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• pp.238-243
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• 2012

국내에서는 다시마 뿌리가 아직 활용되지 않고 있다. 본 연구에서는 다시마 뿌리에서 탄수화물과 미네랄 등을 추출하는 공정을 개발하고 이들의 특성을 측정하였다. 해조류의 다당류 추출은 수율을 높이기 위해서 염산을 많이 사용하는데 본 연구에서는 기능성 식품에 이용하기 위해 구연산과 같은 유기산을 사용해 추출하였다. 유기산은 추출 효율이 강산에 비해 낮으므로 최적의 추출 조건을 찾는 연구를 하였다. 추출온도, 추출시간, 유기산의 농도, 시료 입자 크기 등을 변화시키며 다당 수율을 측정하는 실험을 하였다. 시료 입자가 작을수록 추출 온도가 높을수록 수율은 증가하였다. $100^{\circ}C$에서 구연산 농도 0.2 wt%로 4.0시간 추출했을 때 수율은 19%였다. 다시마뿌리 추출액의 K/Na 비가 약 3으로 칼륨이 많이 함유되어 있었다. 다시마 뿌리에서 추출한 후코이단은 미역에서 추출한 후코이단과 비교해 당의 조성은 비슷하였으나, 분자량은 더 작았다.