• 생명과학회지
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• 제17권10호
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• pp.1330-1335
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• 2007

마늘을 발효시켜 만든 흑마늘의 지질 산화 억제 및 사람 Low Density Lipoprotein (LDL)에 대하여 항산화 활성을 실험하였다. 표준품으로서 마늘의 주성분인 alliin과 흑마늘의 에탄올과 물 추출물의 전자공여능을 각각 비교하여 측정한 결과 흑마늘 에탄을 추출물이 효능이 가장 높았다. 사람 LDL을 $Cu^{2+}$유도 LDL로 산화시킬 때 그 항산화능은 각 시료를 $10\;{\mu}g/ml$ 및 $20\;{\mu}g/ml$ 씩 첨가하며 TSARS를 측정한 결과 에탄을 추출물이 항산화 활성이 가장 높았으며 용량 의존형으로 나타났다. 흑마늘의 에탄올과 물추출물을 $10\;{\mu}g/ml$ 및 $20\;{\mu}g/ml$ 첨가한 후 J774 유도 산화에 대한 항산화 효과를 측정한 결과 각 추출물은 항산화 효과가 있었고 용량 의존형의 항산화 활성을 나타내었다. 흑마늘의 각 추출물을 이용한 실험에서 전기영동 이동상은 대조구에 비하여 $10\;{\mu}g/ml$ 및 $20\;{\mu}g/ml$에서 산화를 억제하였고 공액 2중 결합에 의한 실험에서도 항산화 효과가 있었으며 $20\;{\mu}g/ml$의 농도에서 거의 억제되었다.

• 대한의생명과학회지
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• 제17권2호
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• pp.123-128
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• 2011

We analyzed the concentration of serum total cholesterol (TC), high density lipoprotein cholesterol (HDLC), low density lipoprotein cholesterol (LDLC), triglyceride (TG) and the serum lipoprotein electrophoresis fractions in hypertensive patients (hypertension group, n=182). The average concentration of lipids and lipoprotein fractions in the hypertension group was compared to that of the normal group. The average concentrations of serum TC, LDLC and TG in the hypertension group were significantly higher than those of the normal group (3.3%, 11% and 70%, respectively) (P<0.05). But HDLC was nonsignificantly lower (2%) (P<0.05). In the hypertension group, the percentages of patients who had an abnormally high level of total cholesterol, HDLC, LDLC and TG were 12.1%, 8.2%, 24.2% and 44.5%, respectively. The average of ${\beta}$-lipoprotein (27.5%) and pre ${\beta}$-lipoprotein (12.6%) were significantly higher, whereas ${\alpha}$-lipoprotein (15.2%) was significantly lower in the hypertension group than in the normal group (P<0.05). The percentages of patients who had an abnormally high level of very low density lipoprotein (pre ${\beta}$-LP), LDLC (${\beta}$-LP) and chylomicron were 31.3%, 17.0% and 12.6%, respectively. This study suggests that an increasing of total cholesterol, LDLC and TG, pre ${\beta}$-LP, ${\beta}$-LP and chylomicron are associated with a risk factor for hypertension.

• Childhood Kidney Diseases
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• 제26권1호
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• pp.25-30
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• 2022

Dyslipidemia in nephrotic syndrome (NS) is often characterized by marked increases in the levels of total cholesterol, triglycerides, low-density lipoprotein cholesterol, and other lipoproteins, such as very low-density lipoprotein, intermediate-density lipoprotein, and lipoprotein(a). It has been suggested that impaired catabolism of lipoproteins and cholesterol is mainly due to decreased lipoprotein lipase and hepatic lipase activity, and increased biosynthesis of lipoproteins in the liver. The management strategies for dyslipidemia in patients with NS consist of lifestyle modification, lipid-lowering agents represented by statins, second-line agents such as fibrates and bile acid sequestrants, and lipid apheresis. Compared with dyslipidemia in adult NS patients, whose risks of atherosclerotic disease and progressive renal injury are considered high, clinical data on dyslipidemia in pediatric NS patients are limited. Therefore, it is necessary to pay more attention to the evaluation and management of dyslipidemia in pediatric patients with NS in clinical practice.

• 생약학회지
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• 제27권3호
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• pp.267-273
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• 1996

The methanol extract of Taraxacum coreanum Nakai (Compositae) was examined on the in vitro oxidation of human low density lipoprotein (LDL). It is well known that LDL oxidation induced artherosclerosis, if we can protect LDL oxidation process, excess plasma lipoprotein accumulation into the arterial lesion prone areas can be blocked. The methanol extract was treated with oxidized LDL which was incubated with $16\;{\mu}M$ $Cu^{2+}$ for metal catalyzed oxidation and TBA value, mobility on agarose gel and formation of conjugated diene and change of vitamin E were determined for the evaluation. The extract showed antioxidative effect at concentration $200\;{\mu}g/ml$ on LDL oxidation.

• 한국식품영양학회지
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• 제12권3호
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• pp.320-327
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• 1999

The flavonoids are one of the most numberous and widespread groups of natural consituents. The low molecular weight of benzo-${\gamma}$-pyrone derivative are ubiquitous in plants and are vegetables nuts, seeds, leaves, flowers, and bark. The flavonoids constitute of a large class of compounds ubiquitous in plants containing a number of phenolic hydroxyl groups attached to ring structures conferring the antioxidant activity. Epidemiologic studies suggest that the dietary intake of antioxidants constitutes a risk factor for vasclar disease indicating that oxidation may be important in the pathogenesis of human athero-sclerosis. Elevated plasma low density lipoprotein (LDL) cholesterol concentration are associated with accelerated atherosclerosis, LDL is oxidized by smooth muscle cells resulting in several chemicals and physical changes of LDL. Oxidized LDL is responsible for cholesterol loading of macrophages foam cells formation and atherogenesis. There have been insulficient tests of the protective effects of flavonoids against LDL oxidation to make definitive statements about their structure activity relationships. How-ever hydroxylation of the flavone uncleas can appears to be advantageous because polyhydroxylated aglycone flavonoids are potent inhibitor of LDL modification. This identification may lead to new and more effective antioxidant strategies for abrogating the atherosclerotic process the leading cause of death and disability in industrialized societies.

• Asian-Australasian Journal of Animal Sciences
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• 제32권1호
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• pp.58-62
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• 2019

Objective: The oxidized low density lipoprotein receptor 1 (OLR1) gene plays an important role in the degradation of oxidized low-density lipoprotein and adipocyte proliferation in mammals. For this reason, we aimed at investigating the association of OLR1 gene polymorphisms with carcass quality traits in Chinese Qinchuan cattle. Methods: The single nucleotide polymorphism (SNP) was identified in the 3' untranslated region of bovine OLR1 gene by DNA sequencing. In addition, the haplotype frequency and linkage disequilibrium estimates of three SNPs were evaluated in 520 individuals. Results: Results indicated that the studied three SNPs were within the range of moderate genetic diversity (0.25< polymorphism information content<0.5). Haplotype analysis of three SNPs showed that ten different haplotypes were identified, but only five haplotypes were listed as those with a frequency of <0.05 were excluded. The Hap3 ($-G_1T_2C_3-$) had the highest haplotype frequency (42.10%). Linkage disequilibrium analysis showed that the three SNPs had a low linkage ($r^2<0.001$). The T10588C and C10647T were significantly associated with backfat thickness and intramuscular fat content in Qinchuan cattle. Conclusion: Based on our results, we believe that the OLR1 gene could be a strong candidate gene for influencing carcass quality traits in Qinchuan cattle.

• Food Science and Biotechnology
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• 제18권6호
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• pp.1541-1544
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• 2009

This study evaluated effects of enzymatic hydrolysates of squid on cholesterol lowering in rats. Thirty male rats were blocked into 3 groups [high cholesterol diet (control), 5% normal squid, and 5% enzymatic hydrolysates of squid] and were raised for 10 weeks. Triglyceride level in enzymatic hydrolysates of squid-fed rats was lower than that in the control. Serum low density lipoprotein-cholesterol level followed in the order of control>normal squid>enzymatic hydrolysates. Serum high density lipoprotein-cholesterol level in enzymatic hydrolysates of squid-fed rats was higher than that in control rats. Liver cholesterol level in enzymatic hydrolysates of squid-fed rats was lower than that in control rats.

• Journal of Acupuncture Research
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• 제40권4호
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• pp.351-355
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• 2023

Background: Wet cupping (WC) is an efficient and cost-effective technique for removing metabolic waste from the bloodstream via the skin. The study aimed to examine the effect of WC on cholesterol levels including total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) in a Sudanese population. Methods: In this prospective cross-sectional study, 30 participants undergoing regular WC therapy were enrolled. Blood samples collected twice: pre-WC therapy (case group) and 10-14 days afterward (controls). Results: Of the participants, 56.67% were male and 43.33% were female, aged 24-69. Pre-WC TC and LDL-C levels were significantly higher than the post-WC control group (p = 0.001). Conversely, HDL-C levels decreased significantly in the pre-WC cases compared to controls (p = 0.001). No significant sex-based difference in mean cholesterol levels (p > 0.05). Conclusions: After WC, males and females experienced significant reductions in TC and LDL-C, and significant increase in HDL-C.

• 한국컴퓨터정보학회논문지
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• 제21권12호
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• pp.139-145
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• 2016

In this paper, we propose to evaluate the effect of Resistin-like molecule alpha (Retnla) on the expression of transporters involved in modulating concentrations of peripheral cholesterol and plasma high-density lipoprotein (HDL) cholesterol. High levels of blood cholesterol are a well-recognized risk factor for atherosclerosis and are eliminated via the process of reverse cholesterol transport (RCT). We recently showed that Retnla ameliorates hypercholesterolemia and atherosclerosis by increasing biliary cholesterol secretion, the final step of the process, in low-density lipoprotein receptor-deficient mice. However, the role of Retnla in HDL-mediated cholesterol efflux, initial step of RCT pathway, is not yet clear. To identify cholesterol transport genes regulated by Retnla, we performed an extensive microarray-based gene expression screen using livers from Retnla-overexpressing (Tg) mice and control animals. The most significant change in Retnla-Tg mice was an upregulation of ATP-binding cassette sub-family G member 4 (Abcg4) transport and was validated using quantitative RT-PCR. The validated gene was also induced by treatment of purified Retnla protein in RAW 264.7 cells incubated with acetylated low-density lipoprotein and Hepa1c1c7 cells. Taken together, these results indicates that Retnla might also accelerate initial step of RCT pathway, suggesting therapeutic value of Retnla in the treatment of hypercholesterolemia and atherosclerosis.

• 생명과학회지
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• 제20권11호
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• pp.1595-1602
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• 2010

Low density lipoprotein (LDL)의 산화는 동맥경화의 유발과 진행에 결정적 역할을 하는 것으로 알려져 있다. 본 연구에서는 마늘의 주요 대사산물인 allylmercaptan의 산화 low density lipoprotein에 의해 손상된 내피세포의 보호효과에 대하여 실험하였다. Allylmercaptan의 항산화 활성은 thiobarbituric acid substance (TBARS)로 측정하였다. Allylmercaptan은 0.1, 1 및 10 mM의 농도에서 $Cu^{2+}$에 의해 유도된 LDL의 산화를 용량의존적으로 억제하였다. 폐동맥 내피세포를 $37^{\circ}C$, 5% $CO_2$ 상태에서 24시간 동안 미리 배양시킨 후 세측한 다음 다시 24시간 동안 0.1 mg/ml oxidized LDL (ox-LDL)을 첨가하여 배양하였다. 이 때 ox-LDL이 Lactate dehydrogenase (LDH)의 방출과 glutathione (GSH)를 감소시키는 원인으로 세포막 손상의 지표로 LDH와 GSH 함량을 조사하였다. 본 실험 결과 allylmercaptan을 일정 농도 별로 endothelial cell에 첨가하여 배양했을 때 LDH의 방출과 GSH의 감소를 현저하게 억제하였다. Peroxide를 형광분석법으로 24 well plate에서 직접 측정한 결과 allylmercaptan이 폐동맥 내피세포 내에서 ox-LDL 유도 peroxide의 방출을 억제하였다. 그리고 allylmercaptan은 과산화수소의 소거능도 있었다. 본 실험결과 allylmercaptan은 ox-LDL 유도 폐동맥 내피세포를 보호할 수 있었으므로 allylmercaptan은 동맥경화의 예방에 유용할 것으로 생각된다.