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http://dx.doi.org/10.5713/ajas.18.0079

Analysis of the oxidized low density lipoprotein receptor 1 gene as a potential marker for carcass quality traits in Qinchuan cattle  

Gui, Lin-sheng (State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University)
Raza, Sayed Haidar Abbas (College of Animal Science and Technology, Northwest A&F University)
Jia, Jianlei (State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.32, no.1, 2019 , pp. 58-62 More about this Journal
Abstract
Objective: The oxidized low density lipoprotein receptor 1 (OLR1) gene plays an important role in the degradation of oxidized low-density lipoprotein and adipocyte proliferation in mammals. For this reason, we aimed at investigating the association of OLR1 gene polymorphisms with carcass quality traits in Chinese Qinchuan cattle. Methods: The single nucleotide polymorphism (SNP) was identified in the 3' untranslated region of bovine OLR1 gene by DNA sequencing. In addition, the haplotype frequency and linkage disequilibrium estimates of three SNPs were evaluated in 520 individuals. Results: Results indicated that the studied three SNPs were within the range of moderate genetic diversity (0.25< polymorphism information content<0.5). Haplotype analysis of three SNPs showed that ten different haplotypes were identified, but only five haplotypes were listed as those with a frequency of <0.05 were excluded. The Hap3 ($-G_1T_2C_3-$) had the highest haplotype frequency (42.10%). Linkage disequilibrium analysis showed that the three SNPs had a low linkage ($r^2<0.001$). The T10588C and C10647T were significantly associated with backfat thickness and intramuscular fat content in Qinchuan cattle. Conclusion: Based on our results, we believe that the OLR1 gene could be a strong candidate gene for influencing carcass quality traits in Qinchuan cattle.
Keywords
Carcass Quality Traits; Qinchuan Cattle; Association Analysis; Oxidized Low-density Lipoprotein Receptor 1 Gene;
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1 Sun Y, Lan X, Lei C, Zhang C, Chen H. Haplotype combination of the bovine CFL2 gene sequence variants and association with growth traits in Qinchuan cattle. Gene 2015;563:136-41.   DOI
2 Gui L, Wang H, Wei S, Zhang Y, Zan L. Molecular characterization, expression profiles, and analysis of Qinchuan cattle SIRT1 gene association with meat quality and body measurement traits (Bos taurus). Mol Biol Rep 2014;41:5237-46.   DOI
3 Khatib H, Leonard SD, Schutzkus V, Luo W, Chang YM. Association of the OLR1 gene with milk composition in Holstein dairy cattle. J Dairy Sci 2006;89:1753-60.   DOI
4 Mei C, Wang H, Liao Q, et al. Genetic architecture and selection of Chinese cattle revealed by whole genome resequencing. Mol Biol Evol 2017 Dec 19 [Epub]. 10.1093/molbev/msx322.   DOI
5 Liao CH, Shaw HM, Chao PM. Impairment of glucose metabolism in mice induced by dietary oxidized frying oil is different from that induced by conjugated linoleic acid. Nutrition 2008;24:744-52.   DOI
6 Sawamura T, Kume N, Aoyama T, et al. An endothelial receptor for oxidized low-density lipoprotein. Nature 1997;386:73-7.   DOI
7 Ringseis R, Dathe C, Muschick A, Brandsch C, Eder K. Oxidized fat reduces milk triacylglycerol concentrations by inhibiting gene expression of lipoprotein lipase and fatty acid transporters in the mammary gland of rats. J Nutr 2007;137:2056-61.   DOI
8 Chui PC, Guan HP, Lehrke M, Lazar MA. PPARgamma regulates adipocyte cholesterol metabolism via oxidized LDL receptor 1. J Clin Invest 2005;115:2244-56.   DOI
9 Sun C, Liu C, Zhang Z, et al. Cloning of OLR1 gene in pig adipose tissue and preliminary study on its lipid-accumulating effect. Asian-Australas J Anim Sci 2009;22:1420-8.   DOI
10 Murase T, Kume N, Kataoka H, et al. Identification of soluble forms of lectin-like oxidized LDL receptor-1. Arterioscler Thromb Vasc Biol 2000;20:715-20.   DOI
11 Khatib H, Rosa GJ, Weigel K, et al. Additional support for an association between OLR1 and milk fat traits in cattle. Anim Genet 2007;38:308-10.   DOI
12 Komisarek J, Dorynek Z. Effect of ABCG2, PPARGC1A, OLR1, and SCD1 gene polymorphism on estimated breeding values for functional and production traits in Polish Holstein-Friesian bulls. J Appl Genet 2009;50:125-32.   DOI
13 Shi YY, He L. SHEsis, a powerful software platform for analyses of linkage disequilibrium, haplotype construction, and genetic association at polymorphism loci. Cell Res 2005;15:97-8.   DOI
14 Tanner MS, Sharrard MJ, Rigby AS. Gene polymorphisms and the use of the bonferroni correction factor: when and when not to apply? Arch Dis Child 1997;76:385.   DOI
15 Chen M, Qiu H, Lin X, et al. Lectin-like oxidized low-density lipoprotein receptor (LOX-1) in sickle cell disease vasculopathy. Blood Cells Mol Dis 2016;60:44-8.   DOI
16 Michael V, Khandker I, Chen L, and Li C. SHORT COMMUNICATION: Association analyses of a single nucleotide polymorphism in the promoter of OLR1 with growth, feed efficiency, fat deposition, and carcass merit traits in hybrid, Angus and Charolais beef cattle. Can J Anim Sci 2013;93:193-7.   DOI
17 Fonseca PD, de Souza FR, de Camargo GM, et al. Association of ADIPOQ, OLR1 and PPARGC1A gene polymorphisms with growth and carcass traits in Nelore cattle. Meta Gene 2015;4:1-7.   DOI
18 Nakano M, Mohri T, Fukami T, et al. Single-nucleotide polymorphisms in cytochrome P450 2E1 (CYP2E1) 3'-untranslated region affect the regulation of CYP2E1 by miR-570. Drug Metab Dispos 2015;43:1450-7.   DOI
19 Yie SM, Li LH, Xiao R, Librach CL. A single base-pair mutation in the 3'-untranslated region of HLA-G mRNA is associated with preeclampsia. Mol Hum Reprod 2008;14:649-53.   DOI
20 Schwerin M, Maak S, Hagendorf A, von Lengerken G, Seyfert HM. A 3'-UTR variant of the inducible porcine hsp70.2 gene affects mRNA stability. Biochim Biophys Acta 2002;1578:90-4.   DOI
21 Hou J, An X, Song Y, et al. Two mutations in the caprine MTHFR 3'UTR regulated by microRNAs are associated with milk production traits. PLoS One 2015;10:e0133015.   DOI
22 Lee I, Ajay SS, Yook JI, et al. New class of microRNA targets containing simultaneous 5'-UTR and 3'-UTR interaction sites. Genome Res 2009;19:1175-83.   DOI
23 Bartz M, Kociucka B, Mankowska M, Switonski M, Szydlowski M. Transcript level of the porcine ME1 gene is affected by SNP in its 3'UTR, which is also associated with subcutaneous fat thickness. J Anim Breed Genet 2014;131:271-8.   DOI
24 Juszczuk-Kubiak E, Bujko K, Grzes M, et al. Study of bovine Mef2B gene: the temporal-spatial expression patterns, polymorphism and association analysis with meat production traits. J Anim Sci 2016;94:4536-48.   DOI