• Food Science and Biotechnology
• /
• 제17권2호
• /
• pp.413-416
• /
• 2008

Effects of extracts obtained from the flowers of Elsholtzia splendens on the serum lipid profile and hepatotoxicity in mice were investigated. Female ICR mice were given E. splendens ethanolic extract (ESEs) orally at a dose of 10 or 50 mg/kg BW for 50 days. Significant dose-dependent decreases in triglyceride and low-density lipoprotein (LDL)-cholesterol of serum were observed. In addition, ESEs prolonged the lag-time of LDL oxidation in vitro. In the serum of ICE mice given ESEs orally at 10 and 50 mg/kg BW, the serum levels of aspartate aminotransferase (AST) and lactic dehydrogenase (LDH) increased significantly, while total protein, albumin, creatinine, alanine aminotransferase (ALT), and total bilirubin did not change. Therefore, ESEs may be beneficial to human health, although it has some hepatotoxicity.

• 한국식품조리과학회지
• /
• 제16권6호
• /
• pp.623-628
• /
• 2000

The antioxidative effect of mugwort extracts was measured by DPPH and LDL with four different solutions (70% acetone, ethanol, hot water, cold water). Mugwort extracts contained 3.2% of polyphenol, 380 RE/l00 g of vitamin A, 16.2 mg/100 g of vitamin C, and 5.1 ${\alpha}$-TE/100 g of vitamin E. DPPH revealed the effect in the order of 70% acetone, hot water, ethanol, and cold water. In particular, 70% acetone showed outstandingly stronger activity than the control group. Also, when 10 ${\mu}\ell$ was added, the effect was well noticed. But the antioxidative activity was hardly seen at 15 ${\mu}\ell$. LDL exhibited the same order of strength in proportion to mugwort＇s concentration. Against the control group, the activity of 70% acetone was 7 times, hot water and ethanol 6 times, and cold water 2 times. This result is attributable to the antioxidative increase of polyphenol and antioxidative vitamins.

• 한국생물물리학회:학술대회논문집
• /
• 한국생물물리학회 1996년도 정기총회 및 학술발표회
• /
• pp.44-44
• /
• 1996

Immunoserisor for the determination of LDL(Low-Density Lipoprotein) was developed by using quartz crystal microbalance(QCM) system. LDL is known to be a good indicator for the diagnosis of atherosclerosis and hypercholesterolemia. The immunosensor consists of four parts; detection cell, oscillating circuit, oscilloscope, and frequency counter, and this was interfaced with personal computer for signal processing. (omitted)

• Journal of Microbiology and Biotechnology
• /
• 제32권11호
• /
• pp.1406-1415
• /
• 2022

The formation of macrophage foam cells stimulated by oxidized low-density lipoprotein (ox-LDL) is deemed an important cause of atherosclerosis. Transcription factor Yin Yang 1 (YY1), which is a universally expressed multifunctional protein, is closely related to cell metabolism disorders such as lipid metabolism, sugar metabolism, and bile acid metabolism. However, whether YY1 is involved in macrophage inflammation and lipid accumulation still remains unknown. After mouse macrophage cell line RAW264.7 cells were induced by ox-LDL, YY1 and proprotein convertase subtilisin/kexin type 9 (PCSK9) expressions were found to be increased while low-density lipoprotein receptor (LDLR) expression was lowly expressed. Subsequently, through reverse transcription-quantitative polymerase chain reaction (RT-qPCR), Western blot analysis, Oil Red O staining and cholesterol quantification, it turned out that silencing of YY1 attenuated the inflammatory response and lipid accumulation in RAW264.7 cells caused by ox-LDL. Moreover, results from the JASPAR database, chromatin immunoprecipitation (ChIP) assay, luciferase reporter assay and Western blot analysis suggested that YY1 activated PCSK9 by binding to PCSK9 promoter and modulated the expression of LDLR in the downstream of PCSK9. In addition, the results of functional experiments demonstrated that the inhibitory effects of YY1 interference on ox-LDL-mediated macrophage inflammation and lipid accumulation were reversed by PCSK9 overexpression. To sum up, YY1 depletion inhibited its activation of PCSK9, thereby reducing cellular inflammatory response, cholesterol homeostasis imbalance, and lipid accumulation caused by ox-LDL.

• Journal of Acupuncture Research
• /
• 제25권4호
• /
• pp.41-49
• /
• 2008

Objectives : This study was aimed at examining significant curative effects of Cupping therapy on Cerebral infarction patients with hyperlipidemia. Methods : The subjects in this study were fifty-two patients visited or admitted to Dong-seo Oriental Hospital who broke out with Cerebral infarction from March 2007 through February 2008, with high total cholesterol(240mg/$d\ell$ above) or high triglyceride(200mg/$d\ell$ above) or low density lipoprotein(160mg/$d\ell$ above) Before prescription, they were tested for total cholesterol, triglyceride, low density lipoprotein with fasting and were then rescribed the cupping therapy for 4 weeks(take an average ten times), after which they were retested for total cholesterol, triglyceride, low density lipoprotein. Results : 1. In the high total cholesterol group(triglyceride and low density lipoprotein are normal), statistical significance of total cholesterol changes was recognized before and after the treatment. 2. In the high triglyceride group(total cholesterol and low density lipoprotein are normal), statistical significance of triglyceride changes was recognized before and after the treatment. 3. In the high low density lipoprotein group(total cholesterol and triglyceride are normal), statistical significance of triglyceride changes was recognized before and after the treatment. 4. In the high total cholesterol and high triglyceride group(low density lipoprotein is normal), statistical significance of total cholesterol changes was recognized before and after the treatment. But, triglyceride changes was not that. 5. In the high total cholesterol and high low density lipoprotein group(triglyceride is normal), statistical significance of total cholesterol and low density lipoprotein changes were recognized before and after the treatment. 6. In the high triglyceride and high low density lipoprotein group(total cholesterol is normal), statistical significance of triglyceride changes was recognized before and after the treatment. But, low density lipoprotein changes was not that. 7. In the high total cholesterol and high triglyceride and high low density lipoprotein group, statistical significance of total cholesterol changes and low density lipoprotein changes were recognized before and after the treatment. But, triglyceride changes was not that. Conclusions : Cupping therapy decreased total cholesterol in whole hyperlipidemia group including high total cholesterol.

• Archives of Pharmacal Research
• /
• 제24권5호
• /
• pp.431-436
• /
• 2001

The water extract of Cichorium intybus (WECI) showed a remarkable antioxidative effect on LDL, and inhibitory effects on the production of thiobarbituric acid reactive substance and the Degradation of fatty acids in LDL. Vitamin 1 and unsaturated fatty acids in LDL were protected by adding WECI from the effects of metal catalyzed LDL oxidation. From the results obtained, we conclude that LDL oxidation is inhibited in vitro by the addition of WECI, and that LDL is protected by WECI from oxidative attack, as shown by agarose gel electrohporesis.

• 한국식품영양과학회지
• /
• 제42권6호
• /
• pp.861-868
• /
• 2013

유전적으로 제2형 당뇨병이 유발되는 db/db 생쥐에 OF를 5% 수준으로 4주간 급여한 결과 지방 대사를 개선시켜, 혈장 총콜레스테롤, LDL-콜레스테롤, VLDL-콜레스테롤과 중성지방을 유의적으로(p<0.05) 감소시켰고, HDL-콜레스테롤은 유의적인 차이를 보이지 않았다. 혈장 총콜레스테롤의 감소는 간에서 HMG-CoA-R의 감소에 의한 콜레스테롤의 생성이 줄어든 것이고, 또한 콜레스테롤을 담즙산으로 합성하는 CYP7A1을 증가시켜 콜레스테롤을 담즙산의 형태로 배설시켰기 때문이다. 이 결과 분변 중 담즙산의 함량이 증가하였다. LDL-콜레스테롤의 감소는 LDL-콜레스테롤을 세포로 이동시켜 분해를 촉진하는 LDL-R의 증가로 확인할 수 있었다. db/db mice에서는 비만뿐 아니라 심각한 지방간이 유발되는데 OF의 급여로 AST와 ALT를 낮추어 간세포의 파괴를 완화시켰으며, 간 조직의 H&E 염색 소견에서도 지방간의 완화를 확인할 수 있었다. 또한 대조군으로 C57 생쥐에게 5% 수준으로 OF를 급여한 결과 OF를 급여하지 않은 대조군과 OF를 급여한 실험군의 혈장 중성지방, 총 콜레스테롤, HDL-콜레스테롤, LDL-콜레스테롤, VLDL-콜레스테롤, AST와 ALT 함량의 차이가 없었으며, 간조직 관찰에서도 차이를 나타내지 않았다. 이상의 결과를 종합할 때, OF는 당뇨병이 유발되지 않은 정상혈당의 생쥐에서는 지질대사에 아무런 영향을 주지 않았으며, 제2형 당뇨병인 db/db mice의 당뇨병 합병증인 지질대사 이상을 개선하는데 효과가 있는 것으로 사료되며, OF의 어떤 성분이 이와 같은 효과를 나타냈는지 보다 구체적인 성분 분석과 세부적인 연구를 통한 확인이 필요하다고 사료된다.

• 성인간호학회지
• /
• 제20권6호
• /
• pp.917-931
• /
• 2008

Purpose: This study was aimed to determine the optimum low-density lipoprotein : high-density lipoprotein-cholesterol (LDL : HDL-C) ratio for predicting coronary heart disease(CHD) in Korean people. Methods: It was analyzed this data of 5,431 adults who had undergone health examinations in a hospital in Gyeonggi-do between January 2006 and December 2007. The covariation of the coronary risk factors such as age, HbA1C, systolic blood pressure(SBP), and waist-to-stature ratio(WSR) were analyzed by using logistic regression analysis. Results: The LDL : HDL-C ratio in the male and female groups was mostly distributed between 1.5 and 4.0. The LDL : HDL-C ratio was the most significant cholesterol-related parameter influencing CHD (male: B = .306, p = .054, female : B = .940, p = .010), followed by LDL-C and total cholesterol. It was observed a sharp increase in the odds ratios for LDL : HDL-C ratios of 2.25 - 2.50(male) and 2.00 - 2.25(female). A significant difference was observed in both male(2.25 : $x^2$ = 2.494, p = .072) and female(2.00 : $x^2$ = 413.742, p = .000) groups. Conclusion: The risk level of CHD was set to 2.25 for males and 2.00 for females. Therefore, the optimum LDL : HDL-C ratio for Koreans should be far lower than that for the people in western countries.

• 한국임상수의학회지
• /
• 제29권4호
• /
• pp.277-282
• /
• 2012

본 연구는 건강한 개에서 혈장 homocysteine농도가 비만이나 비만 관련인자(예, 지방, 인슐린, 혈당, 글루카곤, fructosamine)들과 어떤 상관관계가 있는지를 확인하기 위해 실시되었다. 이를 위해, 절식시 혈당, 지방지수(예, total triglycerides [TG], total cholesterol [TC], high-density lipoprotein cholesterol [HDL-C]과 low-density lipoprotein cholesterol [LDL-C]), fructosamine, insulin 및 glucagon 농도를 각각 측정하였다. 실험에 사용된 개들은 body condition score (BCS)에 따라 분류하였다. 평균 혈장 homocysteine 농도는 비만한 개 집단이 정상이나 마른 개 집단에 비해 상당히 높았다. 또한 혈장 homocysteine농도는 HDL-C농도 뿐아니라 TG, TC, 혈장 glucagon 및 fructosamine 농도와도 밀접한 상관관계를 가지고 있었다. 반대로 LDL-C 농도, 절식시 혈당농도 및 insulin농도는 아무런 상관관계가 확인되지 않았다. 본 연구 결과, homocysteine의 농도 상승이 비만견에서 생물학적으로 중요한 역할을 하는 것으로 추정된다.